Contribution of Syngenta Mutants to ABRC
| Seed Donors: | David W. Meinke (Oklahoma State University, Stillwater, OK) |
| David A. Patton (Syngenta, Research Triangle Park, NC) | |
| Donation Date: | March 4, 2007 |
Complete Spreadsheet of Mutants (Excel)
These seeds stocks (1412 recessive seed mutants of Arabidopsis) are being contributed to the Arabidopsis community following the conclusion of a collaborative project involving the Meinke and Patton laboratories that focused on a forward genetic screen of T-DNA insertion lines for recessive mutants with visible defects in seed development and/or seed pigmentation. The T-DNA population screened was NOT the same as the SAIL/GARLIC population generated at TMRI and included in the SALK and TAIR insertion databases. Please do not confuse these separate populations. Many of the mutants included here are NOT tagged with T-DNA. Other mutants are still unresolved with respect to tagging status. These mutants are therefore NOT included in the SeedGenes database. Because genetic complementation tests have not been performed with these mutants and the disrupted genes have not been identified, some of these mutants are likely to be allelic. The 1412 mutants included in this donation therefore do NOT define 1412 distinct genes. Seed stocks were obtained from heterozygous plants identified based on seed phenotype. Approximately 2/3 of the progeny seeds should be heterozygotes. The remainder should be wild-type for the recessive mutation. Because we focused our attention on tagged mutants, terminal phenotypes noted here should be viewed as preliminary and need to be confirmed in future generations.
The initial screening of insertion lines for defects in seed development is described in this publication:
McElver et al. (2001) Insertional mutagenesis of genes required for seed development in Arabidopsis thaliana. Genetics 159: 1751-1763. McElver et al. (2001)
Tzafrir et al. (2004) Identification of genes required for embryo development in Arabidopsis. Plant Physiol. 135: 1206-1220. Tzafrir et al. (2004)
Please cite one or both of these publications when making use of seeds included in this stock donation.
Overall Totals:
| Embryo Defective | 1268 |
| Pigment Defective | 140 |
| Titan (Enlarged Nuclei) * | 4 |
T-DNA Tagging Status:
| Not Tagged | 873 | N |
| Possibly Tagged | 27 | P |
| Unresolved | 462 | U |
| Not Determined | 50 | ND |
Terminal Seed Phenotype: (Requires confirmation; Based on preliminary screening)
| Zygotic | 24 |
| Zygotic/Preglobular | 34 |
| Preglobular | 248 |
| Preglobular/Globular | 83 |
| Globular | 367 |
| Transition | 149 |
| Transition/Cotyledon | 4 |
| Cotyledon | 246 |
| Variable | 94 |
| Albino | 79 |
| Pale Mature | 61 |
| Other | 23 |
T-DNA Vector Used for Transformation:
| CSA101LUC | 3 |
| CSA104 | 489 |
| CSA104LUC | 268 |
| CSA110 | 201 |
| DAP101 | 354 |
| RW1 | 63 |
| SKI15 | 34 |
Selection Agent for Identifying Resistant Plants:
| Basta | 1349 |
| Hygromycin | 63 |
Parental Ecotype:
| Columbia | 1320 |
| WS | 92 |
Parental Genotype:
| Wild-type | 1211 |
| Quartet | 201 |
Footnotes:
* Anyone interested in these 4 lines should contact the Meinke laboratory before seed distribution in order to discuss potential uncertainties about these stocks.
Line numbers followed by ".1" or ".2" were originally part of a double mutant that was separated into single mutants in subsequent generations.
Tagging status was determined by screening resistant transplants for the presence of defective seeds. Details are presented in McElver et al. (2001). Information on parental resistant : sensitive (R:S) ratios, which reflects the number of active inserts present in each parental line, is included with each seed stock. With known tagged mutants (not included in this collection), all resistant transplants were found to be heterozygous for the mutation, consistent with perfect linkage between the T-DNA insert and the seed phenotype. With untagged mutants, lines with a single functional insert yielded a mixture of wild-type and heterozygous plants when screened for the seed phenotype. Mutants classified as possibly tagged yielded a low frequency of resistant wild-type plants, a result that could still be consistent with tagging. Mutants classified as unresolved with respect to tagging status typically contained multiple inserts that interfered with the ability to resolve tagging status through screening of resistant transplants. Based on previous experience with similar insertion lines, we anticipate that about 30% of these "unresolved" mutants are likely to be tagged.