SeedGenes Profile: BIO 2

Gene Information Gene Symbol BIO 2 Chromosome Locus At2g43360   Alleles in Database 1 Identity Confidence Confirmed Alias Symbol EMB 49 (D. Meinke) Alternate Name Gene Class Embryo Defective Predicted Function Biotin Synthase Function Details Catalyzes the final step in biotin synthesis within the mitochondrion Gene Evidence Multigene Disruption D. Meinke (1 allele) Experimental Data D. Meinke (1 allele) Database Release March, 2002 Predicted Sequences Gene: 2488 nucleotides mRNA: 1413 nucleotides Protein:   378 amino acids cDNA Status FLcDNA Available: AY088152 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (93%) Model Genomes:   Neurospora crassa (57%) Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 260548_at

Mutant Information Allele Symbol bio 2 Mutant Class Embryo Defective Source of Mutant D. Meinke Mutant Line Number   Ecotype Columbia Genetic Background   ABRC Stock CS6329 Mutagen Treatment EMS Insertion Mutant No Flanking Sequence   Location of Mutation Entire Gene Deleted Terminal Phenotype Globular  Details Nomarski Phenotype   Images Allelism Evidence Reference Allele Database Release March, 2002 Seed Color White Embryo Color White Percent Mutant Seeds 27.6 % Chi - Square 4.0 Seeds Counted 1135 Percent Top Half 49.2 % Chi - Square <0.1 Average Seed Length 490 ± 30 µm Average Embryo Length 50 ± 40 µm