OSU Syngenta vbi

SeedGenes Profile: EMB 1025

Gene Information
Gene Symbol EMB 1025
Chromosome Locus At4g20090   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function PPR Protein
Function Details Processing of organellar mRNAs
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Single Border Recovery
Vernon / Salk (1 allele)
Database Release March, 2002
Predicted Sequences
Gene:1983 nucleotides
mRNA: 1983 nucleotides
Protein:  660 amino acids
cDNA Status EST Identified
Top BLAST Hits
Arabidopsis: AT5G64320.1 (31%)
Plant ESTs: Rice (Oryza sativa) (42%)
Model Genomes:   None
Subcellular Localization TargetP Targeted to Chloroplasts
SUBA
mRNA Expression Data NASCArray Spot History for 254498_at
    
Mutant Information
Allele Symbol emb 1025-1emb 1025-2
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaVernon / Salk
Mutant Line Number 1822SALK_142675
Ecotype ColumbiaColumbia
Genetic Background   
ABRC Stock CS16008 
Mutagen Treatment T-DNAT-DNA
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence Details  (Help) 
Location of Mutation Exon 1Exon 1
Terminal Phenotype Cotyledon  DetailsCotyledon
Nomarski Phenotype
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release March, 2002December, 2010
Seed Color Pale Green 
Embryo Color Pale Green 
Percent Mutant Seeds 25.5 % 
Chi - Square 0.2 
Seeds Counted 1030 
Percent Top Half 52.5 % 
Chi - Square 0.6 
Average Seed Length 480 ± 50 µm 
Average Embryo Length 280 ± 80 µm