SeedGenes Profile: EMB 1220

Gene Information Gene Symbol EMB 1220 Chromosome Locus At1g60170   Alleles in Database 1 Identity Confidence Not Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function PRP31 Splicing Factor Function Details Pre-mRNA processing (PRP) factor required for spliceosome assembly Gene Evidence Single Border Recovery Meinke / Syngenta (1 allele) Database Release March, 2002 Predicted Sequences Gene: 2628 nucleotides mRNA: 1819 nucleotides Protein:   485 amino acids cDNA Status FLcDNA Available: AY080777 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (91%) Model Genomes:   Homo sapiens (46%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 264221_s_at

Mutant Information Allele Symbol emb 1220 Mutant Class Embryo Defective Source of Mutant Meinke / Syngenta Mutant Line Number 5283 Ecotype Columbia Genetic Background   ABRC Stock CS16014 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation   Terminal Phenotype Cotyledon  Details Nomarski Phenotype   Images Allelism Evidence Reference Allele Database Release March, 2002 Seed Color Pale Yellow-Green Embryo Color Pale Yellow-Green Percent Mutant Seeds 26.6 % Chi - Square 1.6 Seeds Counted 1130 Percent Top Half 50.8 % Chi - Square 0.1 Average Seed Length 530 ± 40 µm Average Embryo Length 230 ± 100 µm