OSU Syngenta vbi

SeedGenes Profile: EMB 1220

Gene Information
Gene Symbol EMB 1220
Chromosome Locus At1g60170   tair
Alleles in Database 1
Identity Confidence Not Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function PRP31 Splicing Factor
Function Details Pre-mRNA processing (PRP) factor required for spliceosome assembly
Gene Evidence
Single Border Recovery
Meinke / Syngenta (1 allele)
Database Release March, 2002
Predicted Sequences
Gene:2628 nucleotides
mRNA: 1819 nucleotides
Protein:  485 amino acids
cDNA Status FLcDNA Available: AY080777
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Brassica napus (91%)
Model Genomes:  Homo sapiens (46%)
Subcellular Localization TargetP Not Targeted to Cell Compartment
SUBA
mRNA Expression Data NASCArray Spot History for 264221_s_at
    
Mutant Information
Allele Symbol emb 1220
Mutant Class Embryo Defective
Source of Mutant Meinke / Syngenta
Mutant Line Number 5283
Ecotype Columbia
Genetic Background  
ABRC Stock CS16014
Mutagen Treatment T-DNA
Insertion Mutant Yes   Details
Flanking Sequence  
Location of Mutation  
Terminal Phenotype Cotyledon  Details
Nomarski Phenotype   Images
Allelism Evidence Reference Allele
Database Release March, 2002
Seed Color Pale Yellow-Green
Embryo Color Pale Yellow-Green
Percent Mutant Seeds 26.6 %
Chi - Square 1.6
Seeds Counted 1130
Percent Top Half 50.8 %
Chi - Square 0.1
Average Seed Length 530 ± 40 µm
Average Embryo Length 230 ± 100 µm