OSU Syngenta vbi

SeedGenes Profile: EMB 1990

Gene Information
Gene Symbol EMB 1990
Chromosome Locus At3g07430   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Uncertain
Function Details Integral membrane, chloroplast division protein
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Single Border Recovery
Meinke / Salk (1 allele)
Database Release March, 2003
Predicted Sequences
Gene:1006 nucleotides
mRNA: 1006 nucleotides
Protein:  232 amino acids
cDNA Status FLcDNA Available: AY099847
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Brassica napus (59%)
Model Genomes:   None
Subcellular Localization TargetP Targeted to Chloroplasts
SUBA
mRNA Expression Data NASCArray Spot History for 259013_at
    
Mutant Information
Allele Symbol emb 1990-1emb 1990-2
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMeinke / Salk
Mutant Line Number 48596SALK_056049
Ecotype ColumbiaColumbia
Genetic Background   
ABRC Stock CS16162CS24080
Mutagen Treatment T-DNAT-DNA
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence Details  (Help) 
Location of Mutation 3Õ UTRExon 1
Terminal Phenotype Cotyledon  DetailsCotyledon  Details
Nomarski Phenotype   Images  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release March, 2003March, 2004
Seed Color Pale YellowPale Yellow
Embryo Color Pale YellowPale Yellow
Percent Mutant Seeds 24.6 %25.6 %
Chi - Square 0.10.3
Seeds Counted 10271101
Percent Top Half 53.0 %60.6 %
Chi - Square 0.912.8
Average Seed Length 520 ± 40 µm500 ± 60 µm
Average Embryo Length 160 ± 90 µm220 ± 100 µm