SeedGenes Profile: EMB 2107

Gene Information Gene Symbol EMB 2107 Chromosome Locus At5g09900   Alleles in Database 1 Identity Confidence Confirmed Alias Symbol RPN 5A (R. Vierstra) Sequence Identity MSA (P. Jenik) Sequence Identity Gene Class Embryo Defective Predicted Function 26S Proteasome Regulatory Subunit (RPN5) Function Details Component of 26S proteasome lid Gene Evidence Border Recovery Meinke / Syngenta (1 allele) Database Release September, 2002 Predicted Sequences Gene: 3318 nucleotides mRNA: 1674 nucleotides Protein:   442 amino acids cDNA Status FLcDNA Available: AY034908 Top BLAST Hits Arabidopsis: AT5G64760.1 (92%) Plant ESTs: Brassica napus (95%) Model Genomes:   Danio rerio (44%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 250456_at

Mutant Information Allele Symbol emb 2107 Mutant Class Embryo Defective Source of Mutant Meinke / Syngenta Mutant Line Number 58351 Ecotype Columbia Genetic Background   ABRC Stock CS16129 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation   Terminal Phenotype Cotyledon  Details Nomarski Phenotype   Images Allelism Evidence Reference Allele Database Release September, 2002 Seed Color Pale Yellow Embryo Color Pale Yellow Percent Mutant Seeds 24.9 % Chi - Square <0.1 Seeds Counted 967 Percent Top Half 53.5 % Chi - Square 1.2 Average Seed Length 520 ± 60 µm Average Embryo Length 300 ± 90 µm