SeedGenes Profile: EMB 2761

Gene Information Gene Symbol EMB 2761 Chromosome Locus At2g04842   Alleles in Database 1 Identity Confidence Not Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Threonine tRNA Synthetase Function Details Translation in mitochondria and chloroplasts Gene Evidence Single Border Recovery Meinke / Salk (1 allele) Database Release October, 2007 Predicted Sequences Gene: 2806 nucleotides mRNA: 1953 nucleotides Protein:   650 amino acids cDNA Status FLcDNA Available: AK221562 Top BLAST Hits Arabidopsis: AT5G26830.1 (39%) Plant ESTs: Rice (Oryza sativa) (80%) Model Genomes:   Synechocystis (54%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA

Mutant Information Allele Symbol emb 2761 Mutant Class Embryo Defective Source of Mutant Meinke / Salk Mutant Line Number SALK_003349 Ecotype Columbia Genetic Background   ABRC Stock   Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Exon 6 Terminal Phenotype Globular  Details Nomarski Phenotype   Images Allelism Evidence Reference Allele Database Release October, 2007 Seed Color White Embryo Color White Percent Mutant Seeds 24.2 % Chi - Square 0.3 Seeds Counted 991 Percent Top Half 51.2 % Chi - Square 0.1 Average Seed Length 500 ± 70 µm Average Embryo Length 70 ± 40 µm