SeedGenes Profile: EMB 2769

Gene Information Gene Symbol EMB 2769 Chromosome Locus At3g13200   Alleles in Database 1 Identity Confidence Not Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function U5 Associated Protein (Cwc15) Function Details Proposed role in pre-mRNA splicing Gene Evidence Single Border Recovery Meinke / Salk (1 allele) Database Release October, 2007 Predicted Sequences Gene: 2021 nucleotides mRNA: 976 nucleotides Protein:   230 amino acids cDNA Status FLcDNA Available: AY086234 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (92%) Model Genomes:   None Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 257192_at

Mutant Information Allele Symbol emb 2769 Mutant Class Embryo Defective Source of Mutant Meinke / Salk Mutant Line Number SALK_010555 Ecotype Columbia Genetic Background   ABRC Stock   Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Intron 4 Terminal Phenotype Preglobular  Details Nomarski Phenotype Globular  Images Allelism Evidence Reference Allele Database Release October, 2007 Seed Color White Embryo Color White Percent Mutant Seeds 18.7 % Chi - Square 18.5 Seeds Counted 863 Percent Top Half 55.9 % Chi - Square 2.2 Average Seed Length 310 ± 110 µm Average Embryo Length 30 ± 50 µm