OSU Syngenta vbi

SeedGenes Profile: EMB 2772

Gene Information
Gene Symbol EMB 2772
Chromosome Locus At5g14800   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Proline Biosynthesis
Function Details Catalyzes final step in proline biosynthesis from glutamate and ornithine
Gene Evidence
Single Border Recovery
Meinke / Salk (2 alleles)
Database Release October, 2007
Predicted Sequences
Gene:1878 nucleotides
mRNA: 1280 nucleotides
Protein:  276 amino acids
cDNA Status FLcDNA Available: AF410303
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Brassica napus (92%)
Model Genomes:  Drosophila melanogaster (48%)
Subcellular Localization TargetP Not Targeted to Cell Compartment
mRNA Expression Data NASCArray Spot History for 246594_at
Mutant Information
Allele Symbol emb 2772-2
Mutant Class Embryo Defective
Source of Mutant Meinke / Salk
Mutant Line Number SALK_098189
Ecotype Columbia
Genetic Background  
ABRC Stock  
Mutagen Treatment T-DNA
Insertion Mutant Yes   Details
Flanking Sequence  
Location of Mutation Intron 5
Terminal Phenotype Preglobular  Details
Nomarski Phenotype Early Globular  Images
Allelism Evidence Complementation Test; Sequence Identity
Database Release October, 2007
Seed Color White
Embryo Color White
Percent Mutant Seeds 24.3 %
Chi - Square 0.3
Seeds Counted 1198
Percent Top Half 56.4 %
Chi - Square 4.7
Average Seed Length 260 ± 60 µm
Average Embryo Length 15 ± 0 µm