SeedGenes Profile: EMB 2779

Gene Information Gene Symbol EMB 2779 Chromosome Locus At4g36480   Alleles in Database 1 Identity Confidence Not Confirmed Alias Symbol LCB 1 (TAIR) Alternate Name FBR 11 (J. Zuo) Sequence Identity Gene Class Embryo Defective Predicted Function Serine Palmitoyltransferase (LCB1 Subunit) Function Details Catalyzes the first step of sphingolipid biosynthesis Gene Evidence Single Border Recovery Meinke / Salk (1 allele) Database Release October, 2007 Predicted Sequences Gene: 3346 nucleotides mRNA: 2058 nucleotides Protein:   482 amino acids cDNA Status FLcDNA Available: AY120759 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Medicago truncatula (75%) Model Genomes:   Homo sapiens (44%) Subcellular Localization TargetP Targeted to Secretory Pathway SUBA mRNA Expression Data NASCArray Spot History for 246213_at

Mutant Information Allele Symbol emb 2779 Mutant Class Embryo Defective Source of Mutant Meinke / Salk Mutant Line Number SALK_077745 Ecotype Columbia Genetic Background   ABRC Stock   Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Exon 2 Terminal Phenotype Preglobular  Details Nomarski Phenotype   Allelism Evidence Reference Allele Database Release October, 2007 Seed Color White Embryo Color White Percent Mutant Seeds 22.0 % Chi - Square 5.1 Seeds Counted 1068 Percent Top Half 61.7 % Chi - Square 12.9 Average Seed Length 480 ± 60 µm Average Embryo Length 15 ± 0 µm