SeedGenes Profile: EMB 2799

Gene Information Gene Symbol EMB 2799 Chromosome Locus At2g39080   Alleles in Database 1 Identity Confidence Not Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Unknown Function Details Potential role in chloroplast nutrient transport Gene Evidence Border Recovery Meinke / Salk (1 allele) Database Release December, 2010 Predicted Sequences Gene: 2744 nucleotides mRNA: 1305 nucleotides Protein:   351 amino acids cDNA Status FLcDNA Available: AY065091 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (82%) Model Genomes:   None Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 266192_at

Mutant Information Allele Symbol emb 2799 Mutant Class Embryo Defective Source of Mutant Meinke / Salk Mutant Line Number SALK_087872 Ecotype Columbia Genetic Background   ABRC Stock   Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Exon 1 Terminal Phenotype Cotyledon  Details Nomarski Phenotype   Allelism Evidence Reference Allele Database Release December, 2010 Seed Color White to Pale Yellow Embryo Color White to Pale Yellow Percent Mutant Seeds 23.5 % Chi - Square 0.3 Seeds Counted 255 Percent Top Half 53.3 % Chi - Square 0.3 Average Seed Length 430 ± 60 µm Average Embryo Length 90 ± 120 µm