SeedGenes Profile: EMB 2810

Gene Information Gene Symbol EMB 2810 Chromosome Locus At1g77470   Alleles in Database 1 Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function DNA Replication Factor (RFC5) Function Details DNA replication Gene Evidence Border Recovery Meinke / SAIL (1 allele) Database Release December, 2010 Predicted Sequences Gene: 2488 nucleotides mRNA: 1468 nucleotides Protein:   369 amino acids cDNA Status FLcDNA Available: AY065452 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (88%) Model Genomes:   Homo sapiens (56%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 259732_at

Mutant Information Allele Symbol emb 2810 Mutant Class Embryo Defective Source of Mutant Meinke / SAIL Mutant Line Number SAIL_401_E05 Ecotype Columbia Genetic Background   ABRC Stock   Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Intron 3 Terminal Phenotype Preglobular Nomarski Phenotype   Allelism Evidence Reference Allele Database Release December, 2010 Seed Color   Embryo Color   Percent Mutant Seeds 24.9 % Chi - Square 0.0 Seeds Counted 385 Percent Top Half   Chi - Square   Average Seed Length   Average Embryo Length