SeedGenes Profile: EMB 3003

Gene Information Gene Symbol EMB 3003 Chromosome Locus At1g34430   Alleles in Database 1 Identity Confidence Not Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Dihydrolipoamide S-Acetyltransferase; Chloroplast Pyruvate Decarboxylase Complex; Putative E3 Binding Protein Function Details Acetyl-CoA formation Gene Evidence Single Border Recovery Meinke / Genoplante (1 allele) Database Release September, 2004 Predicted Sequences Gene: 2441 nucleotides mRNA: 1781 nucleotides Protein:   465 amino acids cDNA Status FLcDNA Available: AY128294 Top BLAST Hits Arabidopsis: AT3G25860.1 (65%) Plant ESTs: Brassica napus (81%) Model Genomes:   Synechocystis (59%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 261165_at

Mutant Information Allele Symbol emb 3003 Mutant Class Embryo Defective Source of Mutant Meinke / Genoplante Mutant Line Number EYP4 Ecotype Wassilewskija Genetic Background   ABRC Stock   Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Exon 1 Terminal Phenotype Preglobular  Details Nomarski Phenotype Globular Allelism Evidence Reference Allele Database Release September, 2004 Seed Color Pale Yellow Embryo Color Pale Yellow Percent Mutant Seeds 23.8 % Chi - Square 0.9 Seeds Counted 1065 Percent Top Half 51.8 % Chi - Square 0.3 Average Seed Length 430 ± 60 µm Average Embryo Length 20 ± 10 µm