OSU Syngenta vbi

SeedGenes Profile: EMB 3003

Gene Information
Gene Symbol EMB 3003
Chromosome Locus At1g34430   tair
Alleles in Database 1
Identity Confidence Not Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Dihydrolipoamide S-Acetyltransferase; Chloroplast Pyruvate Decarboxylase Complex; Putative E3 Binding Protein
Function Details Acetyl-CoA formation
Gene Evidence
Single Border Recovery
Meinke / Genoplante (1 allele)
Database Release September, 2004
Predicted Sequences
Gene:2441 nucleotides
mRNA: 1781 nucleotides
Protein:  465 amino acids
cDNA Status FLcDNA Available: AY128294
Top BLAST Hits
Arabidopsis: AT3G25860.1 (65%)
Plant ESTs: Brassica napus (81%)
Model Genomes:  Synechocystis (59%)
Subcellular Localization TargetP Targeted to Chloroplasts
mRNA Expression Data NASCArray Spot History for 261165_at
Mutant Information
Allele Symbol emb 3003
Mutant Class Embryo Defective
Source of Mutant Meinke / Genoplante
Mutant Line Number EYP4
Ecotype Wassilewskija
Genetic Background  
ABRC Stock  
Mutagen Treatment T-DNA
Insertion Mutant Yes   Details
Flanking Sequence  
Location of Mutation Exon 1
Terminal Phenotype Preglobular  Details
Nomarski Phenotype Globular
Allelism Evidence Reference Allele
Database Release September, 2004
Seed Color Pale Yellow
Embryo Color Pale Yellow
Percent Mutant Seeds 23.8 %
Chi - Square 0.9
Seeds Counted 1065
Percent Top Half 51.8 %
Chi - Square 0.3
Average Seed Length 430 ± 60 µm
Average Embryo Length 20 ± 10 µm