SeedGenes Profile: FIE

Gene Information Gene Symbol FIE Chromosome Locus At3g20740   Alleles in Database 1 Identity Confidence Confirmed Alias Symbol   Gene Class 50% Defective Seeds Predicted Function WD Polycomb Protein Function Details Postulated to interact with MEA (FIS1) and FIS2 proteins to repress gene expression through chromatin structure modulation Gene Evidence Sequenced Mutant Allele R. Fischer (1 allele) Molecular Complementation R. Fischer (1 allele) Database Release March, 2002 Predicted Sequences Gene: 3644 nucleotides mRNA: 1563 nucleotides Protein:   369 amino acids cDNA Status FLcDNA Available: AF129516 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Nicotiana tabacum (75%) Model Genomes:   Homo sapiens (40%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 257987_at

Mutant Information Allele Symbol fie-1 Mutant Class 50% Defective Seeds Source of Mutant R. Fischer Mutant Line Number   Ecotype Landsberg erecta Genetic Background   ABRC Stock   Mutagen Treatment EMS Insertion Mutant No Flanking Sequence   Location of Mutation Intron 3 to Exon 4 Terminal Phenotype Cotyledon  Details Nomarski Phenotype   Images Allelism Evidence Reference Allele Database Release March, 2002 Seed Color White Embryo Color White Percent Mutant Seeds 51.2 % Chi - Square 0.6 Seeds Counted 1136 Percent Top Half 51.2 % Chi - Square 0.3 Average Seed Length 480 ± 40 µm Average Embryo Length 70 ± 60 µm