SeedGenes Profile: FK

Gene Information Gene Symbol FK Chromosome Locus At3g52940   Alleles in Database 1 Identity Confidence Confirmed Alias Symbol HYD 2 (K. Lindsey) Complemetation Test Gene Class Embryo Defective Predicted Function Sterol C-14 Reductase Function Details Sterol biosynthesis during embryo development; functions upstream of STE1/DWF7 in biosynthetic pathway Gene Evidence Sequenced Mutant Allele G. Jurgens (1 allele) Database Release March, 2002 Predicted Sequences Gene: 3099 nucleotides mRNA: 1465 nucleotides Protein:   369 amino acids cDNA Status FLcDNA Available: AY064964 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (92%) Model Genomes:   Homo sapiens (42%) Subcellular Localization TargetP Targeted to Secretory Pathway SUBA mRNA Expression Data NASCArray Spot History for 251995_at

Mutant Information Allele Symbol fk Mutant Class Embryo Defective Source of Mutant G. Jurgens Mutant Line Number   Ecotype Landsberg erecta Genetic Background   ABRC Stock CS8149 Mutagen Treatment EMS Insertion Mutant No Flanking Sequence   Location of Mutation   Terminal Phenotype Cotyledon  Details Nomarski Phenotype   Images Allelism Evidence Reference Allele Database Release March, 2002 Seed Color Pale Green to Green Embryo Color Pale Green to Green Percent Mutant Seeds 22.2 % Chi - Square 4.0 Seeds Counted 989 Percent Top Half 52.7 % Chi - Square 0.7 Average Seed Length 410 ± 30 µm Average Embryo Length 230 ± 70 µm