OSU Syngenta vbi

SeedGenes Profile: HISN 2

Gene Information
Gene Symbol HISN 2
Chromosome Locus At1g31860   tair
Alleles in Database 3   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Histidine Biosynthesis
Function Details Catalyzes the second step in histidine biosynthesis
Gene Evidence
Single Border Recovery
Meinke / Salk (3 alleles)
Database Release October, 2007
Predicted Sequences
Gene:2500 nucleotides
mRNA: 1065 nucleotides
Protein:  281 amino acids
cDNA Status FLcDNA Available: AY086514
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Brassica napus (86%)
Model Genomes:   None
Subcellular Localization TargetP Targeted to Mitochondria
SUBA
mRNA Expression Data NASCArray Spot History for 246265_at
    
Mutant Information
Allele Symbol hisn 2-2
Mutant Class Embryo Defective
Source of Mutant Meinke / Salk
Mutant Line Number SALK_143868
Ecotype Columbia
Genetic Background  
ABRC Stock  
Mutagen Treatment T-DNA
Insertion Mutant Yes   Details
Flanking Sequence  
Location of Mutation Exon 5
Terminal Phenotype Preglobular  Details
Nomarski Phenotype Preglobular  Images
Allelism Evidence Complementation Test; Sequence Identity
Database Release October, 2007
Seed Color White
Embryo Color White
Percent Mutant Seeds 16.0 %
Chi - Square 72.7
Seeds Counted 1672
Percent Top Half 69.3 %
Chi - Square 39.7
Average Seed Length 220 ± 80 µm
Average Embryo Length 15 ± 0 µm