SeedGenes Profile: HISN 2

Gene Information Gene Symbol HISN 2 Chromosome Locus At1g31860   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Histidine Biosynthesis Function Details Catalyzes the second step in histidine biosynthesis Gene Evidence Single Border Recovery Meinke / Salk (3 alleles) Database Release October, 2007 Predicted Sequences Gene: 2500 nucleotides mRNA: 1065 nucleotides Protein:   281 amino acids cDNA Status FLcDNA Available: AY086514 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (86%) Model Genomes:   None Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 246265_at

Mutant Information Allele Symbol hisn 2-2 Mutant Class Embryo Defective Source of Mutant Meinke / Salk Mutant Line Number SALK_143868 Ecotype Columbia Genetic Background   ABRC Stock   Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Exon 5 Terminal Phenotype Preglobular  Details Nomarski Phenotype Preglobular  Images Allelism Evidence Complementation Test; Sequence Identity Database Release October, 2007 Seed Color White Embryo Color White Percent Mutant Seeds 16.0 % Chi - Square 72.7 Seeds Counted 1672 Percent Top Half 69.3 % Chi - Square 39.7 Average Seed Length 220 ± 80 µm Average Embryo Length 15 ± 0 µm