SeedGenes Profile: HISN 2

Gene Information Gene Symbol HISN 2 Chromosome Locus At1g31860   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Histidine Biosynthesis Function Details Catalyzes the second step in histidine biosynthesis Gene Evidence Single Border Recovery Meinke / Salk (3 alleles) Database Release October, 2007 Predicted Sequences Gene: 2500 nucleotides mRNA: 1065 nucleotides Protein:   281 amino acids cDNA Status FLcDNA Available: AY086514 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (86%) Model Genomes:   None Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 246265_at

Mutant Information Allele Symbol hisn 2-3 Mutant Class Embryo Defective Source of Mutant Meinke / Salk Mutant Line Number SALK_055503 Ecotype Columbia Genetic Background   ABRC Stock   Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation 5' UTR Terminal Phenotype Cotyledon  Details Nomarski Phenotype   Images Allelism Evidence Complementation Test; Sequence Identity Database Release October, 2007 Seed Color White to Pale Yellow Embryo Color White to Pale Yellow Percent Mutant Seeds 23.9 % Chi - Square 1.3 Seeds Counted 2117 Percent Top Half 55.9 % Chi - Square 7.1 Average Seed Length 340 ± 100 µm Average Embryo Length 110 ± 140 µm