SeedGenes Profile: LEC 2

Gene Information Gene Symbol LEC 2 Chromosome Locus At1g28300   Alleles in Database 1 Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function B3 Domain Transcription Factor Function Details B3 domain similar to ABI3, VP1, and FUS3 Gene Evidence Sequenced Mutant Allele J. Harada (1 allele) Molecular Complementation J. Harada (1 allele) Database Release March, 2002 Predicted Sequences Gene: 3010 nucleotides mRNA: 1317 nucleotides Protein:   363 amino acids cDNA Status FLcDNA Available: AF400123 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (68%) Model Genomes:   None Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 245669_at

Mutant Information Allele Symbol lec 2-1 Mutant Class Embryo Defective Source of Mutant Meinke / Feldmann Mutant Line Number CS2728 Ecotype Wassilewskija Genetic Background   ABRC Stock CS2728 Mutagen Treatment T-DNA Insertion Mutant No Flanking Sequence   Location of Mutation Deletion 5' to Exon 2 Terminal Phenotype Cotyledon  Details Nomarski Phenotype   Allelism Evidence Reference Allele Database Release March, 2002 Seed Color Pale Yellow-Green Embryo Color Pale Yellow-Green Percent Mutant Seeds 23.0 % Chi - Square 1.5 Seeds Counted 1024 Percent Top Half 47.7 % Chi - Square 0.5 Average Seed Length 510 ± 50 µm Average Embryo Length 410 ± 70 µm