OSU Syngenta vbi

SeedGenes Profile: LEC 2

Gene Information
Gene Symbol LEC 2
Chromosome Locus At1g28300   tair
Alleles in Database 1
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function B3 Domain Transcription Factor
Function Details B3 domain similar to ABI3, VP1, and FUS3
Gene Evidence
Sequenced Mutant Allele
J. Harada (1 allele)
Molecular Complementation
J. Harada (1 allele)
Database Release March, 2002
Predicted Sequences
Gene:3010 nucleotides
mRNA: 1317 nucleotides
Protein:  363 amino acids
cDNA Status FLcDNA Available: AF400123
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Brassica napus (68%)
Model Genomes:   None
Subcellular Localization TargetP Not Targeted to Cell Compartment
SUBA
mRNA Expression Data NASCArray Spot History for 245669_at
    
Mutant Information
Allele Symbol lec 2-1
Mutant Class Embryo Defective
Source of Mutant Meinke / Feldmann
Mutant Line Number CS2728
Ecotype Wassilewskija
Genetic Background  
ABRC Stock CS2728
Mutagen Treatment T-DNA
Insertion Mutant No
Flanking Sequence  
Location of Mutation Deletion 5' to Exon 2
Terminal Phenotype Cotyledon  Details
Nomarski Phenotype  
Allelism Evidence Reference Allele
Database Release March, 2002
Seed Color Pale Yellow-Green
Embryo Color Pale Yellow-Green
Percent Mutant Seeds 23.0 %
Chi - Square 1.5
Seeds Counted 1024
Percent Top Half 47.7 %
Chi - Square 0.5
Average Seed Length 510 ± 50 µm
Average Embryo Length 410 ± 70 µm