OSU Syngenta vbi

SeedGenes Profile: PDE 326

Gene Information
Gene Symbol PDE 326
Chromosome Locus At3g03710   tair
Alleles in Database 4   See List OR Show All
Identity Confidence Confirmed
Alias Symbol RIF 10 (M. Rodgriguez-C) Sequence Identity
Gene Class Seed Pigment
Predicted Function Plastid Polynucleotide Phosphorylase
Function Details Proposed role in 23S rRNA 3' end processing
Gene Evidence
Border Recovery
Meinke / Salk (2 alleles)
Border Recovery
Rodriguez-Concepcion / Weigel (1 allele)
Border Recovery
Rodriguez-Concepcion / Salk (1 allele)
Database Release December, 2010
Predicted Sequences
Gene:5797 nucleotides
mRNA: 3103 nucleotides
Protein:  922 amino acids
cDNA Status FLcDNA Available: AJ252123
Top BLAST Hits
Arabidopsis: AT5G14580.1 (42%)
Plant ESTs: Rice (Oryza sativa) (73%)
Model Genomes:  Escherichia coli (40%)
Subcellular Localization TargetP Targeted to Chloroplasts
SUBA
mRNA Expression Data NASCArray Spot History for 259344_at
    
Mutant Information
Allele Symbol pde 326-1
Mutant Class Seed Pigment
Source of Mutant Meinke / Salk
Mutant Line Number SALK_070705
Ecotype Columbia
Genetic Background  
ABRC Stock  
Mutagen Treatment T-DNA
Insertion Mutant Yes   Details
Flanking Sequence  
Location of Mutation Exon 12
Terminal Phenotype Pigment Defective  Details
Nomarski Phenotype  
Allelism Evidence Reference Allele
Database Release December, 2010
Seed Color Pale Green
Embryo Color Pale Green
Percent Mutant Seeds 27.1 %
Chi - Square 0.6
Seeds Counted 251
Percent Top Half 48.5 %
Chi - Square 0.1
Average Seed Length 550 ± 0 µm
Average Embryo Length 400 ± 0 µm