SeedGenes Profile: PDE 326

Gene Information Gene Symbol PDE 326 Chromosome Locus At3g03710   Alleles in Database 4   See List OR Show All Identity Confidence Confirmed Alias Symbol RIF 10 (M. Rodgriguez-C) Sequence Identity Gene Class Seed Pigment Predicted Function Plastid Polynucleotide Phosphorylase Function Details Proposed role in 23S rRNA 3' end processing Gene Evidence Border Recovery Meinke / Salk (2 alleles) Border Recovery Rodriguez-Concepcion / Weigel (1 allele) Border Recovery Rodriguez-Concepcion / Salk (1 allele) Database Release December, 2010 Predicted Sequences Gene: 5797 nucleotides mRNA: 3103 nucleotides Protein:   922 amino acids cDNA Status FLcDNA Available: AJ252123 Top BLAST Hits Arabidopsis: AT5G14580.1 (42%) Plant ESTs: Rice (Oryza sativa) (73%) Model Genomes:   Escherichia coli (40%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 259344_at

Mutant Information Allele Symbol pde 326-2 Mutant Class Seed Pigment Source of Mutant Meinke / Salk Mutant Line Number SALK_037353 Ecotype Columbia Genetic Background   ABRC Stock   Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Intron 5 Terminal Phenotype Pigment Defective  Details Nomarski Phenotype   Allelism Evidence Complementation Test; Sequence Identity Database Release December, 2010 Seed Color Pale Green Embryo Color Pale Green Percent Mutant Seeds 27.9 % Chi - Square 1.0 Seeds Counted 222 Percent Top Half 59.7 % Chi - Square 2.3 Average Seed Length 550 ± 0 µm Average Embryo Length 400 ± 0 µm