OSU Syngenta vbi

SeedGenes Profile: PDE 326

Gene Information
Gene Symbol PDE 326
Chromosome Locus At3g03710   tair
Alleles in Database 4   See List OR Show All
Identity Confidence Confirmed
Alias Symbol RIF 10 (M. Rodgriguez-C) Sequence Identity
Gene Class Seed Pigment
Predicted Function Plastid Polynucleotide Phosphorylase
Function Details Proposed role in 23S rRNA 3' end processing
Gene Evidence
Border Recovery
Meinke / Salk (2 alleles)
Border Recovery
Rodriguez-Concepcion / Weigel (1 allele)
Border Recovery
Rodriguez-Concepcion / Salk (1 allele)
Database Release December, 2010
Predicted Sequences
Gene:5797 nucleotides
mRNA: 3103 nucleotides
Protein:  922 amino acids
cDNA Status FLcDNA Available: AJ252123
Top BLAST Hits
Arabidopsis: AT5G14580.1 (42%)
Plant ESTs: Rice (Oryza sativa) (73%)
Model Genomes:  Escherichia coli (40%)
Subcellular Localization TargetP Targeted to Chloroplasts
mRNA Expression Data NASCArray Spot History for 259344_at
Mutant Information
Allele Symbol pde 326-2
Mutant Class Seed Pigment
Source of Mutant Meinke / Salk
Mutant Line Number SALK_037353
Ecotype Columbia
Genetic Background  
ABRC Stock  
Mutagen Treatment T-DNA
Insertion Mutant Yes   Details
Flanking Sequence  
Location of Mutation Intron 5
Terminal Phenotype Pigment Defective  Details
Nomarski Phenotype  
Allelism Evidence Complementation Test; Sequence Identity
Database Release December, 2010
Seed Color Pale Green
Embryo Color Pale Green
Percent Mutant Seeds 27.9 %
Chi - Square 1.0
Seeds Counted 222
Percent Top Half 59.7 %
Chi - Square 2.3
Average Seed Length 550 ± 0 µm
Average Embryo Length 400 ± 0 µm