SeedGenes Profile: TWN 2

Gene Information Gene Symbol TWN 2 Chromosome Locus At1g14610   Alleles in Database 1 Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Valyl tRNA Synthetase Function Details Translation in mitochondria or cytoplasm Gene Evidence Molecular Complementation C. Somerville (1 allele) Double Border Recovery C. Somerville (1 allele) Database Release March, 2002 Predicted Sequences Gene: 6290 nucleotides mRNA: 3632 nucleotides Protein:   1108 amino acids cDNA Status FLcDNA Available: U89986 Top BLAST Hits Arabidopsis: AT5G16715.1 (40%) Plant ESTs: Rice (Oryza sativa) (67%) Model Genomes:   Drosophila melanogaster (50%) Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 260780_at

Mutant Information Allele Symbol twn 2 Mutant Class Embryo Defective Source of Mutant Somerville / Feldmann Mutant Line Number CS2644 Ecotype Wassilewskija Genetic Background   ABRC Stock CS8840 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation 5' UTR Terminal Phenotype Cotyledon  Details Nomarski Phenotype   Images Allelism Evidence Reference Allele Database Release March, 2002 Seed Color Pale Yellow Embryo Color Pale Yellow Percent Mutant Seeds 22.5 % Chi - Square 3.2 Seeds Counted 947 Percent Top Half 60.6 % Chi - Square 9.6 Average Seed Length 460 ± 40 µm Average Embryo Length 230 ± 140 µm