OSU Syngenta vbi

SeedGenes Profile: TWN 2

Gene Information
Gene Symbol TWN 2
Chromosome Locus At1g14610   tair
Alleles in Database 1
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Valyl tRNA Synthetase
Function Details Translation in mitochondria or cytoplasm
Gene Evidence
Molecular Complementation
C. Somerville (1 allele)
Double Border Recovery
C. Somerville (1 allele)
Database Release March, 2002
Predicted Sequences
Gene:6290 nucleotides
mRNA: 3632 nucleotides
Protein:  1108 amino acids
cDNA Status FLcDNA Available: U89986
Top BLAST Hits
Arabidopsis: AT5G16715.1 (40%)
Plant ESTs: Rice (Oryza sativa) (67%)
Model Genomes:  Drosophila melanogaster (50%)
Subcellular Localization TargetP Targeted to Mitochondria
mRNA Expression Data NASCArray Spot History for 260780_at
Mutant Information
Allele Symbol twn 2
Mutant Class Embryo Defective
Source of Mutant Somerville / Feldmann
Mutant Line Number CS2644
Ecotype Wassilewskija
Genetic Background  
ABRC Stock CS8840
Mutagen Treatment T-DNA
Insertion Mutant Yes   Details
Flanking Sequence  
Location of Mutation 5' UTR
Terminal Phenotype Cotyledon  Details
Nomarski Phenotype   Images
Allelism Evidence Reference Allele
Database Release March, 2002
Seed Color Pale Yellow
Embryo Color Pale Yellow
Percent Mutant Seeds 22.5 %
Chi - Square 3.2
Seeds Counted 947
Percent Top Half 60.6 %
Chi - Square 9.6
Average Seed Length 460 ± 40 µm
Average Embryo Length 230 ± 140 µm