SeedGenes Profile: VCL 1

Gene Information Gene Symbol VCL 1 Chromosome Locus At2g38020   Alleles in Database 1 Identity Confidence Confirmed Alias Symbol MAN (S. Gillmor) Alternate Name Gene Class Embryo Defective Predicted Function Vacuolar Protein Sorting (Vps16) Function Details Required for vacuole biogenesis and protein trafficking to the vacuole Gene Evidence Molecular Complementation C. Somerville (1 allele) Border Recovery C. Somerville (1 allele) Database Release March, 2002 Predicted Sequences Gene: 4803 nucleotides mRNA: 2875 nucleotides Protein:   858 amino acids cDNA Status FLcDNA Available: AY048248 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Rice (Oryza sativa) (55%) Model Genomes:   Danio rerio (35%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 266096_at

Mutant Information Allele Symbol vcl 1 Mutant Class Embryo Defective Source of Mutant Somerville / Feldmann Mutant Line Number   Ecotype WS/COL Genetic Background   ABRC Stock CS3941 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Exon 4 Terminal Phenotype Preglobular  Details Nomarski Phenotype Preglobular  Images Allelism Evidence Reference Allele Database Release March, 2002 Seed Color White Embryo Color White Percent Mutant Seeds 20.1 % Chi - Square 13.6 Seeds Counted 1082 Percent Top Half 49.5 % Chi - Square <0.1 Average Seed Length 390 ± 50 µm Average Embryo Length 15 ± 0 µm