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At2g26830 SYNGENTA 12935

Genomic Sequence (from TAIR)

ttcgtcaaaaagattttattgatagaatgtgggatcgatttctaatgactgttatttttgtttctaggttctgataaaaatattttttagttaaaatcag
tattgctttctgactttctcagacatcacaatctcataatacatcgtgagagatagattcaaacatccctaattccaaacttgtacggtatgtgttaaat
taaaaacttaaaacgactgatccctaatatcaaaatgccgacataaaatataaatggaagcgttgaattagggaaaaaaaacagaatcaaaattaattga
gatctgattacaaaaaaaaaaaaagagaatctttatttgtatttgcagaagaagaagagaaaaagtttgaaagaaaATGGGAGCAGCGAAGAATATCTGG
GCATTAGCCAACGCCGAAGATGCAGCTAACGACGCCGAACAAATCCCTTATTCTTCGTTCGTCGTCGACACGTCTTTGCCTCTTCCTCTCATGATTCCTC
GTATCATgttcgtttccttatctaattttcttctcatttgatagctcgttccgtgaattttggagatttgattttgttttcctccttgaaattgggattg
aagaagaagatctctctgacctagaacttacaaattggcttttattgctattttctttacacttggattggttttgtggaggcagtagttgttgtaaagt
tgctttgatggtgacatagtgaaagcttcctttatcttcttgttaagactgcttcgttgtgttacttatgagattaaaataacattaaaaagatgataag
actgcttcgttgtgtgactcatcttcttgttattgcttcgttttcatggcggattagttagaaatgaatggaatgtggcatgagtcttgtttctctagga
agagatatgtaaaagctatcttgaacctttgttttggttgtaatttctctttttttttgtaacagAGAGTTATGTAAAGATCTGTTCAAGAATTGGGGAG
AGCTTGATGATTCACTCTTCTCTGTTGAGAGAGTATCTGGAGGCATTACAAATCTTTgtgagtttcccttacaaggttacagtaattacatgtcaatgct
catattttctaccttggttctataactgttatgtcatcttttccatgatggcagTGCTGAAGGTTTCTGTGAAAGAGGACACTAATAAAGAAGTGTCTGT
TACAGTGAGACTGTATGGGCCTAACACTGAGTATGTTATTAACCGGGAGAGAGAGATTCTGgtaatttcctttatttatatatatgataagtcccttccc
ccttgtgttctgatccgtgtttttggtgtctttgtttgcgttaccaagagtttctatgttatttaaattatttttcttctagtgatgtcaacttgaagca
tgagccttacgtcagaacatgggagagttttgctcgattttttcttgaaatgtcaaaggtttctctgccagtgttattcttttgcatcatttgatgtttt
atcatttgtgtttttcgttatttgctccccacgtcttcaatagGCTATCAAGTATCTCTCGGCTGCCGGATTTGGTGCCAAGTTGCTTGGTGGTTTTGGA
AATGGCATGGTGCAATCGTTCATCAATGCAAGAACCTTAGAGCCATCAGgtaggtattttaaattccagtagcgggaatgtctctagaaaacataaattg
cttcttgttgttaagggagtgaatttccttgaaatttcgtatggttaataaattcaaatgaaagtttgccgccacaagacaaaaaaaaaaacaaatggat
aagcttcaatccttgtctagaatacttctatgctatggaattaaactgaatgaagttgtttttaagatttggcaatgctcctcattaacagtggtggaac
atctcaaatgaacctcatttctcctttgttgtgggcagACATGAGAGAGCCAAAGATTGCTGCACAAATTGCCAGAGAACTTGGAAAGTTTCATAAAGTG
GACATACCAGGTTCCAAGGAACCTCAGCTGTGGGTTGATATCTTGAAGTTCTATGAAAAAGgttataagtttatttttgttttgattattgttctagttt
cttttccccttatttgatgaagtgt◄tggctagatca►taaaccacc►aatagtggttccagctcttatgcgtttttccatgtctgctatgtacctttac
ttgcagCATCTACTCTTACGTTTGAAGAACCTGATAAGCAGAAGCTCTTTGAGACTATTTCGTTTGAAGAACTCCACAAAGAAATTATTGAGCTAAGGgt
atttccttctgctctgtaaagaattttgtatctctgcactgtgtatatgttcttgatgtcttatgatactccatttctggtgtttgaaaatcctactagt
caagttctgcctaggtgcgaatatggtttaccacataattgtagttgtttttgtgctatgtgctaaaccagacaatacaatgaatactatattttttcat
cttggaaattgtagttgcttgtgtgctatgtgctaaactgtggccatccttgagtgatgtcgaagaattggtcctctggttcttctcctatgcagtaact
tgttccaaaatctgctaattcttctttcagtaatctaaagtatttgtgtttgtgattggttgtgcatgttagcaatctattctttgctttggtttgattg
tatttgctgattctgatgattgatcagGAATTCACAGGCTTACTTAACGCACCTGTGGTGTTTGCTCATAACGATTTACTATCAGGAAACTTCATGCTGA
ATGATGAAGAAGgtaagaaaatcccttcttctctgtacttctttctcggctgaaagaacagaaatataatgacagacatagacacgaaaaagtagggtca
ctttattacccgaaaatttgcactttagatacaagatcccagaaccaactttatgagttgggcaacaattgtcttcttctgtatcgtaagtagttatgat
atctagtttctgacttgattctcttcatccatattcagAGAAACTATACTTGATTGATTTCGAGTATGGATCATACAACTATAGAGGCTTTGACATCGGA
AATCACTTCAATGAATATGCAGGATACGACTGTGATTACAGCCTgtatgttcaacttccttgacttcttctgttgtctctaaaaatttcctcaacagatc
aaaacataattgctcattgtggtttctttgtagGTACCCAAGTAAAGAGGAACAATATCATTTCATCAAGCATTACTTACAGCCAGACAAACCAGACGAG
gtaagaacatatcagcgtttggaattaatacaatttgtttttaatggagatagtaatatgttgcaagacattacgtctgtgttcttttgaaacaattttg
gcttaagaaatatatataaattgctggtgtaaacagGTCAGTATCGCTGAAGTAGAATCAGTCTTTGTAGAGACAGATGCGTATAAATTAGCATCTCATT
TGTACTGGGCAATATGGGCGATCATCCAGgtgcgcttgttaaaaccatctagtatattgctttatgttagtaacttgtattaagctaatgtaactggtac
aaatttggtggaacagGCAAGGATGTCTCCCATTGAGTTTGAATATTTGGGTTACTTCTTTTTGCGGTACAATGAATACAAGAAGCAGAAGCCACTTACT
TTTTCACTTGTTACATCTCACCTCTCAGCATCGCTGTAGcctttggttggccaaaatggttaaatctttttttgttttttttgttgtgttgttatcaatt
cttgtaaaatttgtttgtgttgttagttaaaccttgtatgatttgtatgaatgaacttgatttgtggaagtagttgcactttatattaactatcacccac
aagcaaggtgctaaggcctaaggaggatgaataagccaatttggagtctattataatcctccttcacttgaatagttggaattgtaattgtttgattaag
gatttactaactggtaaaaaaaaagatttaccaactggtttagcaaaaatacatttagtagagttaggaatagactccaaattgtaattgtttgattaat
gagttgagagccaagaaactaaaacaCTATTTAACCTCCACTAACTGGTTTTCTAAGTAGTTAACTTGAGAGAGTAATTCCTTTGGCTGGATCAACAATT
TCT

Border_1 (12935_LB2D)


AATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTACACTTCATCAAATAAGGGGAAAAGAAACTAGAACAATA
ATCAAAACAAAAATAAACTTATAACCTTTTTCATAGAACTTCAAGATATCAACCCACAGCTGAGGTTCCTTGGAACCTGGTATGTCCACTTTATGAAACT
TTCCAAGTTCTCTGGCAATTTGTGCAGCAATCTTTGGCTCTCTCATGTCTGCCCACAACAAAGGAGAAATGAGGTTCATTTGAGATGTTCCACCACTGTT
AATGAGGAGCATTGCCAAATCTTAAAAACAACTTCATTCAGTTTAATTCCATAGCACAGGACTTWT

Start of BLAST Alignment:	NT 1749 / Intron 5 of 10
First 15 bp Perfect Match:	NT 1749 / Intron 5 of 10

BLAST Details:
Query Sequence: 12935_LB2D 
Length: (366 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At2g26830 68415.t03007 choline/ethanolamine kinase family...  1485  9.5e-62   1
At5g45380 68418.t05189 sodium:solute symporter family pro...   178  0.86      1
At1g03950 68414.t00334 SNF7 family protein contains Pfam ...   173  0.97      1
At1g63855 68414.t06621 expressed protein                       172  0.98      1


>At2g26830 68415.t03007 choline/ethanolamine kinase family protein similar to
           SP|Q9HBU6 Ethanolamine kinase (EC 2.7.1.82) (EKI){Homo sapiens},
           GmCK3p [Glycine max] GI:1438883; contains Pfam profile PF01633:
           Choline/ethanolamine kinase
        Length = 3546

  Minus Strand HSPs:

 Score = 1485 (228.9 bits), Expect = 9.5e-62, P = 9.5e-62
 Identities = 300/305 (98%), Positives = 301/305 (98%), Strand = Minus / Plus

Query:   366 AWAAGTCCTGTGCTATGGAATTAAACTGAATGAAGTTGTTTTTAAGATTTGGCAATGCTC 307
             |+ | | || ||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1483 AATACTTCTATGCTATGGAATTAAACTGAATGAAGTTGTTTTTAAGATTTGGCAATGCTC 1542

Query:   306 CTCATTAACAGTGGTGGAACATCTCAAATGAACCTCATTTCTCCTTTGTTGTGGGCAGAC 247
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1543 CTCATTAACAGTGGTGGAACATCTCAAATGAACCTCATTTCTCCTTTGTTGTGGGCAGAC 1602

Query:   246 ATGAGAGAGCCAAAGATTGCTGCACAAATTGCCAGAGAACTTGGAAAGTTTCATAAAGTG 187
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1603 ATGAGAGAGCCAAAGATTGCTGCACAAATTGCCAGAGAACTTGGAAAGTTTCATAAAGTG 1662

Query:   186 GACATACCAGGTTCCAAGGAACCTCAGCTGTGGGTTGATATCTTGAAGTTCTATGAAAAA 127
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1663 GACATACCAGGTTCCAAGGAACCTCAGCTGTGGGTTGATATCTTGAAGTTCTATGAAAAA 1722

Query:   126 GGTTATAAGTTTATTTTTGTTTTGATTATTGTTCTAGTTTCTTTTCCCCTTATTTGATGA 67
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1723 GGTTATAAGTTTATTTTTGTTTTGATTATTGTTCTAGTTTCTTTTCCCCTTATTTGATGA 1782

Query:    66 AGTGT 62
             |||||
Sbjct:  1783 AGTGT 1787


>At5g45380 68418.t05189 sodium:solute symporter family protein contains Pfam
           profile: PF00474 sodium:solute symporter family
        Length = 4703

  Minus Strand HSPs:

 Score = 178 (32.8 bits), Expect = 2.0, P = 0.86
 Identities = 106/173 (61%), Positives = 106/173 (61%), Strand = Minus / Plus

Border_2 (12935_LB1U)


TTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTACACCGCAATAGTGGTTCCAGCTCTTATGC
GTTTTTCCATGTCTGCTATGTACCTTTACTTGCAGCATCTACTCTTACGTTTGAAGAACCTGATAAGCAGAAGCTCTTTGAGACTATTTCGTTTGAAGAA
CTCCACAAAGAAATTATTGAGCTAAGGGTATTTCCTTCTGCTCTGTAAAGAATTTTGTATCTCTGCACTGTGTATATGTTCTTGATGTCCTATGCTACTC
CAAA

Start of BLAST Alignment:	NT 1760 / Intron 5 of 10
First 15 bp Perfect Match:	NT 1769 / Intron 5 of 10

BLAST Details:
Query Sequence: 12935_LB1U 
Length: (304 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At2g26830 68415.t03007 choline/ethanolamine kinase family...  1119  5.3e-45   1
At2g10190 68415.t00994 gypsy-like retrotransposon family ...   183  0.021     1
At2g33205 68415.t03762 TMS membrane family protein / tumo...   178  0.032     1
At3g16550 68416.t01876 DegP protease, putative contains s...   162  0.98      1


>At2g26830 68415.t03007 choline/ethanolamine kinase family protein similar to
           SP|Q9HBU6 Ethanolamine kinase (EC 2.7.1.82) (EKI){Homo sapiens},
           GmCK3p [Glycine max] GI:1438883; contains Pfam profile PF01633:
           Choline/ethanolamine kinase
        Length = 3546

  Plus Strand HSPs:

 Score = 1119 (173.9 bits), Expect = 5.3e-45, P = 5.3e-45
 Identities = 229/234 (97%), Positives = 229/234 (97%), Strand = Plus / Plus

Query:    70 TACACCGC-AATAGTGGTTCCAGCTCTTATGCGTTTTTCCATGTCTGCTATGTACCTTTA 128
             || ||| | |||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1799 TAAACCACCAATAGTGGTTCCAGCTCTTATGCGTTTTTCCATGTCTGCTATGTACCTTTA 1858

Query:   129 CTTGCAGCATCTACTCTTACGTTTGAAGAACCTGATAAGCAGAAGCTCTTTGAGACTATT 188
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1859 CTTGCAGCATCTACTCTTACGTTTGAAGAACCTGATAAGCAGAAGCTCTTTGAGACTATT 1918

Query:   189 TCGTTTGAAGAACTCCACAAAGAAATTATTGAGCTAAGGGTATTTCCTTCTGCTCTGTAA 248
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1919 TCGTTTGAAGAACTCCACAAAGAAATTATTGAGCTAAGGGTATTTCCTTCTGCTCTGTAA 1978

Query:   249 AGAATTTTGTATCTCTGCACTGTGTATATGTTCTTGATGTCCTATGCTACTCCA 302
             ||||||||||||||||||||||||||||||||||||||||| |||| |||||||
Sbjct:  1979 AGAATTTTGTATCTCTGCACTGTGTATATGTTCTTGATGTCTTATGATACTCCA 2032


>At2g10190 68415.t00994 gypsy-like retrotransposon family (Athila) has a
           2.9e-120 P-value blast match to gb|AAL06422.1|AF378081_1 reverse
           transcriptase (Athila4) [Arabidopsis thaliana] (Gypsy_Ty3-family)
        Length = 6574

  Plus Strand HSPs:

 Score = 183 (33.5 bits), Expect = 0.021, P = 0.021
 Identities = 143/246 (58%), Positives = 143/246 (58%), Strand = Plus / Plus