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At4g29060 SYNGENTA 129824

Genomic Sequence (from TAIR)
CAAACTCTCCTTCTTCACTCTGAGAAAGTGCTTTGTTGGAGAAGAATTGCAGCACCAGAAGAAGCACTAGTACAAAAAATTTCATcgttttgcaattctt
ttttcctctcatgaatgatcttaccataagtttttctttttttggtcaaaaccatatgattataatatgaaaaagatctaaataggctttatctgtatga
cttggagggtcaaaaactaaagtcttttagactcttagtaataaaatgttgaataagtcttgttcgacgcctcttattgatttattaagaaattgaccag
catgaaaaggaggcattgctactatgtaatgtctgggggggagtttcacatcgatataggctattgatgggccttagtatgaagcccgtaagcccatatt
gaagctcgagtcttgatggcgcaggagagagagaaaaagacaatcacctgtagtaatctttgaagactcttatctcctctatccaccaagagagggaaaa
cgaaataaaaatagcacaggggagagacaaaatgagataaggatttgaagcctctcaccactctcctcgttttcgcttcgtgcttatttgtcaaacgcga
acatttcaccgaaacttagcgacttttctcgctttactctttctgggagcaatttctcagagcataggcgagcttaaaggtagctccttttcttagcttt
gctcaaatgggtttcttagttactcttcttatgtttccgtgataagatggttcgaaatttactagctttcttaatggaaatttttgagtttttctagtta
gactacaatgaaatttcaattcgaaattgagaaaaagagtggtccttttcttcatatgctttcagattagtctgtgttgttgtctgaggaaagcttgtaa
ttttgacagtgtgtaatggttttctatgtgtaggtgacagttcttagctATGGCTACGATTACACCTTCTTCTATTAGCAATGCCTGGCTCATTCCTGGA
GCTTCTTTTACTGTAAAGAAGAGTGATTGTTCCATCAAATGTAGCTTTTCGCGTAAAGCTGGGAAACAGATTCTATCATCAACGCAGAGACTTGTGTTAC
CTTTGTCGACTTCACTTAGATTGTTTCCTACACATGGAAGGCAGTTTGTGTTGCATCCTCATCGTAGAGCTACTGGGACTGATGTTGTGGCGGCTGTAGA
GGAACAAGATTCTACTCCTGTTGTTGCGGAGGATAAAGAAACCGTGGCTAGTGAGAAGTCAGATGCTCCTGCTCCAACATCTCAGTCAAGGGGAACAGCA
AGACCTGGAAGGAAGAGTGAGATGCCTGCGGTTAAGAACGAGGAGCTGGTTCCTGGAGCTACTTTCACTGGGAAAGTACGAGCTATTCAGCCTTTTGGAG
CTTTTGTTGATTTTGGTGCTTTTACTGATGGATTAGTCCATGTTTCTCAATTGAGTGACAACTTTGTTAAGGATGTTTCGAGTGTTGTCACTATTGGACA
AGAGGTGAAGGTAAGGCTAGTGGAAGCTGACATTGAGTCAAAACGTATTTCTTTAACTATGCGTGAGAATGATGATCCTCCTAAGCGTCAGTCTGGTGGT
AGTGATAAGCCGAGATCAGGAGGGAAAAGGGATGGTTCTAAAGGAGGAGGACAGAGGAAGGGAGAAGGGTTTAACTCAAAGTTTGCCAAAGGGCAGATGT
TGGATGGAGTGGTCAAGAATTTAACGAGGTCAGGAGCGTTTATAACTATAGGAGAAGGCGAAGAAGGGTTTTTACCGACGGCTGAGGAAGCAGATGATGG
TATTGGAAGCATGATGATGGGTGGCTCTTCGTTGCAAGCTGGACAAGAGGTGAAGGTTCGTGTGTTGAGGATAGCAAGGGGACGTGTTACTTTAACAATG
AAAGAGGAAGATGATGGTAAGTTTGATGAAACAACCACTCAGGGAGTTGTGCATACAGCCACAAATCCGTTTATGCTTGCTTTCCGTAAGAACGAAGAGA
TTGCTGCGTTTCTTGACAAGAGGGAGGAAGAGGCAGAGAAGCCACCTGTTGAGACACCAGTAGAGCCTGAAGCTGAAGCCTCTGTCACCTCAGCTGAAGT
CGAGGAATCGGTATGTGTCCCAGCTGAAGTAACAAGTGAAGAGGTTCCCTCGTCAGAAACTCCCAAGGTAGTAGAAGAAGAAGTGATAGCTACCAAAGCT
GAAGATGATTCGCCGGAAAAAGAAGAGCAAACGGAAACACTTGCTGCAGCAGCTGAAGCTGAAGAGGTGGTTCCTCCAATTCCAGAAACCAAAAgtaagt
tgactaaaaatcactagattgtaattttttttgtgtgctacttctcatattggtttattatgatcagtatcagtttatcagaatcatttaaaaagattat
acaatgtttgtatcttagaactctatccaaggtaacatatacagctaaagcgttggggatactccaaattatagtagagaccttaaccacataaatagtc
acatgaactacgtgttctaaaaaaatccagaataatcaaattcatgaagttttgtttgatggagcaaaaggtgataagaaacatataagaccacaaagat
aaagacattttagttcattgtgtaatgattactagcattgacccttttcttctaccggtgaccagGTGAAGAGGAAATTGTTGAGAACTCTATTCCTCCA
AACTCAGCCACTGATGAAGTTTCATCTCCAGAAGCTTTAGCAAGTGAAGAGGTTGAGAAGGAGCAAGTGGTTGCCGAAACACCTGTTGATGAAGTCAAGA
CTCCTGCACCTGTAGTCACAGAAGCTTCTTCTGAGGAAAGTGGCAACACCGCTACTGCTGAAAGCATCAAAGgtccttcttttgtccatcctttacctgt
tttacttcctgactcatgacctaatggtttatgtgagattccatctcctaacacaaaaatgtaacagGCATTTCACCAGCTCTAGTGAAGCAGCTGAGAG
AAGAAACAGGGGCAGGAATGATGGACTGCAAGAATGCTCTTTCAGAGAGTGAGGGCGATATGGTCAAAGCTCAAGAGTACCTCCGCAAGAAGGGACTAGC
GAGCGCAGATAAGAAAGCAAGCAGAGCCACATCCGAGGGAAGAATTGGTGCTTATATCCACGACAGCAGAATCGGTGTCCTCTTGGAGGTTAATTGTGAG
ACTGATTTTGTCTCACGTGGCGACATTTTCAAGGAACTTGTTGATGATCTGGCGATGCAGgtaactccaagtttctagctgaaacattttcttcgttttg
ctataactgagaatataatttgacctgacatgtagGTGGCTGCGTGTCCTCAAGTAGAGTATCTTGTAACAGAAGATGTTTCAGAAGAAATTGTGAAGAA
AGAAAAAGAGATAGAGATGCAAAAGGAAGATCTTTTGTCGAAACCGGAACAAATAAGGGAGAAGATAGTGGACGGTCGGATAAAGAAAAGGCTAGATTCA
CTTGCATTGCTTGAGCAACCATACATTAAAGACGATAAGGTGATAGTTAAGGATCTTGTAAAGCAGAGGATTGCAACCATTGGAGAAAACATCAAAGTGA
AGAGATTTGTGAGGTACACTCTCGGAGAAGGCCTTGAGAAGAAAAGCCAGGACTTTGCTGCTGAGGTTGCTGCCCAAACTGCAGCTAAACCGAAAGCTAA
AGAAGAGCCAAAAGCTGAAGAGGCCAAGGAAGCTGTTGCAAGgtgagaaattttttatagtctttgctcttcttgacatgaattcttataatgttggcta
tagaaagattcaaactttttgcaatacggcactgcagCCCACCAACTACAGTGGTTTCAGCTGCTCTTGTAAAGCAACTGCGTGAAGAGACAGGAGCTGG
AATGATGGATTGCAAGAAGGCATTGGCGGCGACAGGAGGAGATCTTGAGAAAGCACAAGAATTCCTCAGAAAGAAGGGTCTCTCATCAGCTGATAAAAAA
TCAAGCCGGCTTGCATCAGAAGGGAGAATCGGTTCGTACATCCACGATTCTCGGATTGGAGTTCTGATAGAAGTGAACTGTGAGACTGACTTTGTCGGAA
GAAGCGAAAAATTCAAGGAATTGGTTGATGATCTTGCAATGCAAGCAGTGGCTAATCCACAGgtaagaaaatgtcctcagctgaaacaatggcataaatt
ggttgttggtctctgacctgaaatgtctacgtgcagGTGCAATATGTTTCAATAGAGGACATTCCAGAGGAGATAAAGCAGAAAGAAAAAGAGATTGAGA
TGCAAAGAGAGGATCTTTTGTCGAAACCAGAGAACATAAGGGAGAAGATTGTGGAAGGAAGAATCTCAAAGAGGCTTGGAGAATGGGCATTGCTTGAG
CAGCCTTACATCAAAGACGACAGTGTTCTGGTGAAGGATCTGGTGAAGCAAACAGTGGCTACTCTTGGGGAAAACATCAAAGTCAGAAGGTTTGTGAAGT
TTACTCTTGGAGAAGATAACTGAtgagacagacgaagtaaagttttcaaataagggagctctgttttgtgtcatggagtttaaagtaaaagtcttccttt
ttgttttgtagacaaaatcttcagtattcacattttgattagtttttaatcaaaacctgtaattttttgttcatgaaaatatgagatttgcattatataa
atgtgcatttagtgacaatgtgacattatacactaaattcggagaaagattggttataatgaaattttaaaccttattttaaatagaagtttcaaatg
aatttccctgaagaagtgaaactaaataaaataattttttgtttccttttggatattatatctaaatataaaaatcaaaccgaccgaattactacataca
ctacaataatgtttttttccttttataatttgtgggctaaaaaaggtcggtcggcccattttgagcccaataaaatttagggatagataaagacataaag
taacgaacgatattccgaggttgtcatttgctaaggaaaaaaaaaacgaaaacgtgtgtctgtctcttctcgtagcgtctctcaagctcaggttcgtgaa
catt

Border_1 (129824_LBD)

CTAGCATCTGAATTTCATAACCAATCTCGATACACCAAATCGAATTCAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTG
TCTAAGCGTCAATTTGTTTACACCACAATATATCCTGAATCTCAATCTCTTTTTCTTTCTGCTTTATCTCCTCTGGAATGTCCTCTATTGAAACATATTG
CACCTGCACGTAGACATTTCAGGTCAGAGACCAACAACCAATTTATGCCATTGTTTCAGCTGAGGACATTTTCTTACCTGTGGATTAGCCACTGCTTGCA
TTGCAAGATCATCAACCAATTCCTTGAATTTTTCGCTTCTTCCGACAAAGTCAGTCTCACAGTTCACTTCTATCAGAACTCCAATCCGAGAATCGTGGAT
GTACGAACCGATTCTCCCTTCTGATGCAAGCCGGCTTGATTTTTTATCAGCTGATGAGAGACCCTTCTTTCTGAGGAATTCTTGTGCTTTCTCAAGATCT
CCTCCTGTCGCCGCCAATGCCTTCTTGCAATCCATCATTCCAGCTCCTGTCTCTTCACGCAGTTGCTTTACAAGAGCAGCTGAAACCACTGTAGTTGGTG
GGCTGCAGTGCCGTATTGCAAAAAGTTTGAATC:TTTCTATAGCCAACATTATAAGAATTCATGTCAA:GAAGAGCAAAGACTATAAAAAA:TTTCTCAC
CTTGCAACAGCTTCCTTGGCCTCTTCA:GCTTTT:GGCTCTTCTTTAGCTTTCGGTTTAGCTGCAGTTTGGGCAGCAACCTCAGMAGCAAAGTCCTGGCT
TTTCTTCTCAAGGCCTTCTCCGAGAGTGG

Start of BLAST Alignment:NT 3380 / Exon 7 of 7
First 15 bp Perfect Match:NT 3352 / Exon 7 of 7
BLAST Details:
Query Sequence: 129824_LBD 
Length: (824 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At4g29060 68417.t03858 elongation factor Ts family protei...  3435  4.5e-151  1
At4g11120 68417.t01714 translation elongation factor Ts (...   237  0.027     1
At3g06500 68416.t00685 beta-fructofuranosidase, putative ...   187  0.999     1
At5g40760 68418.t04607 glucose-6-phosphate 1-dehydrogenas...   186  0.9994    1


>At4g29060 68417.t03858 elongation factor Ts family protein similar to
           SP|P35019 Elongation factor Ts (EF-Ts) {Galdieria sulphuraria};
           contains Pfam profiles PF00627: UBA/TS-N domain, PF00889: Elongation
           factor TS, PF00575: S1 RNA binding domain
        Length = 4312

  Minus Strand HSPs:

 Score = 3435 (521.4 bits), Expect = 4.5e-151, P = 4.5e-151
 Identities = 700/713 (98%), Positives = 701/713 (98%), Strand = Minus / Plus

Query:   823 CACTCTCGGAGAAGGCCTTGAGAAGAAAAGCCAGGACTTTGCTKCTGAGGTTGCTGCCCA 764
             |||||||||||||||||||||||||||||||||||||||||||+||||||||||||||||
Sbjct:  3156 CACTCTCGGAGAAGGCCTTGAGAAGAAAAGCCAGGACTTTGCTGCTGAGGTTGCTGCCCA 3215

Query:   763 AACTGCAGCTAAACCGAAAGCTAAAGAAGAGCCAAAAGCTGAAGAGGCCAAGGAAGCTGT 704
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  3216 AACTGCAGCTAAACCGAAAGCTAAAGAAGAGCCAAAAGCTGAAGAGGCCAAGGAAGCTGT 3275

Query:   703 TGCAAGGTGAGAAATTTTTTATAGTCTTTGCTCTTCTTGACATGAATTCTTATAATGTTG 644
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  3276 TGCAAGGTGAGAAATTTTTTATAGTCTTTGCTCTTCTTGACATGAATTCTTATAATGTTG 3335

Query:   643 GCTATAGAAAGATTCAAACTTTTTGCAATACGGCACTGCAGCCCACCAACTACAGTGGTT 584
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  3336 GCTATAGAAAGATTCAAACTTTTTGCAATACGGCACTGCAGCCCACCAACTACAGTGGTT 3395

Query:   583 TCAGCTGCTCTTGTAAAGCAACTGCGTGAAGAGACAGGAGCTGGAATGATGGATTGCAAG 524
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  3396 TCAGCTGCTCTTGTAAAGCAACTGCGTGAAGAGACAGGAGCTGGAATGATGGATTGCAAG 3455

Query:   523 AAGGCATTGGCGGCGACAGGAGGAGATCTTGAGAAAGCACAAGAATTCCTCAGAAAGAAG 464
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  3456 AAGGCATTGGCGGCGACAGGAGGAGATCTTGAGAAAGCACAAGAATTCCTCAGAAAGAAG 3515

Query:   463 GGTCTCTCATCAGCTGATAAAAAATCAAGCCGGCTTGCATCAGAAGGGAGAATCGGTTCG 404
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  3516 GGTCTCTCATCAGCTGATAAAAAATCAAGCCGGCTTGCATCAGAAGGGAGAATCGGTTCG 3575

Query:   403 TACATCCACGATTCTCGGATTGGAGTTCTGATAGAAGTGAACTGTGAGACTGACTTTGTC 344
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  3576 TACATCCACGATTCTCGGATTGGAGTTCTGATAGAAGTGAACTGTGAGACTGACTTTGTC 3635

Query:   343 GGAAGAAGCGAAAAATTCAAGGAATTGGTTGATGATCTTGCAATGCAAGCAGTGGCTAAT 284
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  3636 GGAAGAAGCGAAAAATTCAAGGAATTGGTTGATGATCTTGCAATGCAAGCAGTGGCTAAT 3695

Query:   283 CCACAGGTAAGAAAATGTCCTCAGCTGAAACAATGGCATAAATTGGTTGTTGGTCTCTGA 224
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  3696 CCACAGGTAAGAAAATGTCCTCAGCTGAAACAATGGCATAAATTGGTTGTTGGTCTCTGA 3755

Query:   223 CCTGAAATGTCTACGTGCAGGTGCAATATGTTTCAATAGAGGACATTCCAGAGGAGATAA 164
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  3756 CCTGAAATGTCTACGTGCAGGTGCAATATGTTTCAATAGAGGACATTCCAGAGGAGATAA 3815

Query:   163 AGCAGAAAGAAAAAGAGATTGAGATTCAG-GATAT-AT-TGTGGTGTAAACAA 114
             ||||||||||||||||||||||||| ||  || |  || | | ||  |||| |
Sbjct:  3816 AGCAGAAAGAAAAAGAGATTGAGATGCAAAGAGAGGATCTTTTGTCGAAACCA 3868


>At4g11120 68417.t01714 translation elongation factor Ts (EF-Ts), putative
           similar to ethylene-responsive elongation factor EF-Ts precursor
           [Lycopersicon esculentum] GI:5669636; contains Pfam profile PF00889:
           Elongation factor TS
        Length = 2363

  Minus Strand HSPs:

 Score = 237 (41.6 bits), Expect = 0.028, P = 0.027
 Identities = 121/201 (60%), Positives = 121/201 (60%), Strand = Minus / Plus


Border_2 (129824_RBU)

TTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAAWTTYACAC
AGGAAACAGCTATGACCATGATTACGCCAAGCTCGAAATTAACCCTCACTAAAGGGAACAAAAGCTGGAGCTCGCTTGAGCTTGGATCAGATTGTCGTTT
CCCGCCTTCAGTTTATGAAGCGTCATTTAAATAGAAGTTTCAAATGAATTTCCCTGAAGAAGTGAAACTAAATAAAATAATTTTTTGTTTCCTTTTGGAT
ATTATATCTAAATATAAAAATCAAACCGACCGAATTACTACATACACTACAATAATGTTTTTTTCCTTTTATAATTTGTGGGCTAAAAAAGGTCGGTCGG
CCCATTTTGAGCCCAATAAAATTTAGGGATAGATAAAGACATAAAGTAACGAACGATATTCCGAGGTTGTCATTTGCTAAGGAAAAAAAAANCAAAANCG
GGGGTNTGTCTNTTNTCGAAGCGNCTNTNAAGCTNAGGTTCGGNANCATTCCAATNTNTNAANGCAAAAGCTNATTCAAATNATCCTTNTNTGTTATTTT
CNAAATCCNATTNACTNTTNTTTACNAGCNAATTNTAGGGTTTTGAGNATNGATNTTTC

Start of BLAST Alignment:NT > 241 / 3’ UTR
First 15 bp Perfect Match:NT > 254 / Downstream
BLAST Details:
Query Sequence: 129824_RBU 
Length: (659 letters) 
Database: TIGR AGI BAC Sequences (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

F25O24 67173                                                  1836  9.4e-77   1
F19B15 67174                                                  1836  9.4e-77   1
T6C20 68339                                                    674  2.9e-24   1
T2P3 68413                                                     669  4.9e-24   1


F19B15 67174
        Length = 101,679

  Plus Strand HSPs:

 Score = 1836 (281.5 bits), Expect = 9.4e-77, P = 9.4e-77
 Identities = 394/447 (88%), Positives = 394/447 (88%), Strand = Plus / Plus

Query:   213 TTATGAAGCGTCATTTAAATAGAAGTTTCAAATGAATTTCCCTGAAGAAGTGAAACTAAA 272
             || | || | |  |||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 33164 TTTTAAACCTTATTTTAAATAGAAGTTTCAAATGAATTTCCCTGAAGAAGTGAAACTAAA 33223

Query:   273 TAAAATAATTTTTTGTTTCCTTTTGGATATTATATCTAAATATAAAAATCAAACCGACCG 332
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 33224 TAAAATAATTTTTTGTTTCCTTTTGGATATTATATCTAAATATAAAAATCAAACCGACCG 33283

Query:   333 AATTACTACATACACTACAATAATGTTTTTTTCCTTTTATAATTTGTGGGCTAAAAAAGG 392
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 33284 AATTACTACATACACTACAATAATGTTTTTTTCCTTTTATAATTTGTGGGCTAAAAAAGG 33343

Query:   393 TCGGTCGGCCCATTTTGAGCCCAATAAAATTTAGGGATAGATAAAGACATAAAGTAACGA 452
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 33344 TCGGTCGGCCCATTTTGAGCCCAATAAAATTTAGGGATAGATAAAGACATAAAGTAACGA 33403

Query:   453 ACGATATTCCGAGGTTGTCATTTGCTAAGGNNNNNNNNNNCAAAANCGGGGGTNTGTCTN 512
             ||||||||||||||||||||||||||||||          | ||| || | || ||||| 
Sbjct: 33404 ACGATATTCCGAGGTTGTCATTTGCTAAGGAAAAAAAAAACGAAAACGTGTGTCTGTCTC 33463

Query:   513 TTNTCGAAGCGNCTNTNAAGCTNAGGTTCGGNANCATTCCAATNTNTNAANGCAAAAGCT 572
             || ||| |||| || | ||||| |||||||  | ||||||||| | | || |||||||||
Sbjct: 33464 TTCTCGTAGCGTCTCTCAAGCTCAGGTTCGTGAACATTCCAATCTCTCAATGCAAAAGCT 33523

Query:   573 NATTCAAATNATCCTTNTNTGTTATTTTCNAAATCCNATTNACTNTTNTTTACNAGCNAA 632
              |||| ||| |||||| | |||||||||| | |||| ||| ||| || ||||| ||  ||
Sbjct: 33524 CATTCGAATTATCCTTCTCTGTTATTTTCGAGATCCGATTTACTCTTCTTTACTAGTCAA 33583

Query:   633 TTNTAGGGTTTTGAGNATNGATNTTTC 659
             || |||||||||||| || ||| ||||
Sbjct: 33584 TTCTAGGGTTTTGAGTATTGATCTTTC 33610


T6C20 68339
        Length = 80,593

  Minus Strand HSPs:

 Score = 674 (107.2 bits), Expect = 2.9e-24, P = 2.9e-24
 Identities = 226/320 (70%), Positives = 228/320 (71%), Strand = Minus / Plus