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At3g04870 SYNGENTA 13528

Genomic Sequence (from TAIR)
actaaaagtttagtaaaaaatcgtttcaatctttttcttatctaagtaccatctaaaatgggtttaggctcaattcttcacctaatttcatttcgaatag
gttttgatcatataaaaaagaaaaaaaattggttaagcgcactgaagacagttgttagcaccacagagttttatgaggagagaaagcatcatacatcatc
acaaacaagtttcttcatcgctcattgttagtcattagtactttacttcttccttcttcctcttttattataagaagagaaagaaaaaaacatcatcttt
tttttcttgaataacagagcgattgctcttatctcagttgattggccATGGCTTCTTCAGTCGTCTTCGCGGCAACTGGGTCTCTCTCCGTTCCTCCATT
GAAGTCTCGGAGATTCTATGTTAATTCTTCACTCGACTCCGATGTTTCCGACATGAGTGTTAACGgtaattaaccaaacaaaaattgaattctttactgt
ctcctctgcgtcaggttgattcctttggatgtttcggattctgggtatttcaattggattctttagaaaatcggtaaattttgtctttttagtggaaaat
ttgagaaattgacatgtactaggattaaaaggcttagctttatgccacgaacaatcccacttttgaagaggatgatgtttcaattctcatggcatattga
tgaactcaatctatttatttcctggcatatagatgaattctgaactctcatggtttagataaaaagatgaaactcttgtggcttctctctactaatggca
tagtaatggaaatggagagtttggtcgagattttacggtcgatccaagattctcttttgtttatataatcagtttagtttatccatgggaagtagtagct
gttaaggaatcaaatttagatttaaggtttcatgttatgagaagctttcttcttctaataattttctggagcatcgtgattcttgatcatctcttactgt
ttaaatggtctggttatgaatacagCTCCAAAGGGGTTATTTCCTCCAGAGCCTGTACCATACAAGGGACCTAAGCTGAAAGTAGCCATTATAGGAGCTG
GACTTGCAGGCATGTCGACTGCTGTAGAGCTTCTGGATCAGGGTCACGAGgtgtttactcctcttctgttagttgtttatatatttgatttggttacata
catgtgtaatcatcgtgattgtttcagGTGGATATATATGATTCGAGGACATTCATTGGTGGCAAAGTGGGTTCTTTTGTAGATAGACGTGGGAACCATA
TTGAAATGGGATTACATGTTTTCTTCGGATGCTACAACAATCTCTTCCGTTTGATGAAAAAGgtttttgtttctcaaagcttctgctcaaatttacttga
tgattgaaactcatactcatgtatggttttttttgtggtgatgtagGTGGGTGCTGAGAAAAACCTTCTAGTCAAAGACCATACTCATACATTTATAAA
CAAAGACGGCACAATTGGAGgtatggctcagttcattattctcttgctattgtgattttatcgtactgagatgtgcatattatttgtagAGCTTGATTT
TCGGTTTCCTGTTGGAGCTCCAATCCATGGTATTCGCGCATTTTTGGTCACAAATCAACTCAAGgtataaatcaaacttttaaattgatttatcttttaa
atctttggattcatatcaatgcacttttccgccgtggaaaatcggcaacagttttctcaaatgttcatagcaaaagattagtaatttcttgctttatgta
ccaatatcgagcttgctaactgcttattcttgttttgaccgaacagCCCTATGACAAACTAAGGAATTCACTGGCTCTTGCATTAAGCCCTGTTGTTAAA
GCTCTTGTCGACCCCGATGGAGCAATGAGAGACATTCGCAACCTTGACAGCgtaaagaatgataacctgttcttcttctcatctgtactactatttgtgt
tctctaaattttctgaaatactttttgcagATAAGTTTCTCGGATTGGTTCCTGTCAAAAGGTGGGACACGGGCAAGTATCCAGAGAATGTGGGATCCTG
TTGCTTATGCTCTAGGTTTCATAGACTGCGATAACATGAGTGCGCGATGCATGCTTACAATATTCTCATTATTCGCTACCAAAACAGAAGCTTCTCTGTT
GCGTATGCTCAAGGGCTCTCCTGATGTTTACTTGAGTGGTCCTATCAAACAATATATCACAGATAGAGGTGGCAGgtgtgtatctgctataactacaaga
actgattctcgcatttattatgtcattggcttatcgggtatttataatattgtcttcaaacagAATCCATCTAAGGTGGGGTTGCAGGGAGATACTTTAT
GATAAATCAGCAGATGGAGAAACTTATGTCACTGGACTAGCAATTTCTAAGgtgaaatttagcttcagacgtaggattttagtggactgcgaaaatgtat
acatacattctgaaggttgcagcttctgggtagagaaaaagcacaaaactttgatatgaaagtaaaggactttttttgttttttggtttgctttcagGCT
ACGAACAAGAAGATTGTGAAAGCTGATGTATATGTTGCAGCATGTGATGTGCCGGGAATCAAAAGGTTACTTCCAAAAGAATGGAGGGAATCTCGGTTTT
TCAACGATATATATGAACTCGAGGGAGTACCTGTGGTTACAGTACAGCTCAGATACAATGGTTGGGTCACGGAGTTACAAGACATTGAACTTGCTAGgtt
ggtaatctagaatcctatgttttgggatgatcgtgatggatctattctgttttggttttaagtctttgtttttgtgttttggaaaatcgtagGCAGTTGA
AACGAGCGGTTGGCTTAGATAATCTTCTCTACACTCCTGATGCTGATTTCTCGTGCTTTGCGGATCTTGCACTAGCTTCGCCTGCAGATTATTACATCGA
AGGACAAGGCACTTTACTTCAgtaagaaagaataacgtttttctttgtttacaaaatcgcatgcaggtctctcagtgtcttgaaactgaattctgttttc
acagGTGCGTTCTAACACCAGGAGATCCGTATATGCGAATGCCAAATGATAAGATCATAGAGAAAGTAGCGATGCAGgtaaagaacatggctaaattcaa
ctcttgagaaaacttttgtctagctggtttgttgttgattctcaaatcttgttcagGTCACTGAGTTGTTTCCATCGTCACGAGGCCTAGAAGTCACTTG
GTCGTCGGTTGTAAAAATCGCTCAGTCTCTGTACCGTGAAGCACCCGGAAAAGATCCATTCAGACCCGATCAGAAGACGCCCATAAAGAATTTCTTCCTC
GCCGGTTCATACACAAAGCAGgtaacatttttcacttactatctttcagccagatccccgatagctacaaatgcaagcaagaacagcctccaaattgtgt
ctgagaaatccgaaagatcagtgagaattttgtttctggacttgttacagGATTACATAGATAGTATGGAAGGAGCAACATTATCAGGGAGACAAGCTTC
GTCATACATCTGTGATGCTGGTGAAGAACTAGCAGAGCTAAACAAGAAGCTTTCTTCTTCTGCAACTGCAGTTCCTGATGAGCTAAGTCTGGTCTAAtga
tgaaaaatgtttgaaagtagttaccaatggctaattgatgaagatttgagacactatgggtacatgaagaagaagcatatgtagaaaccctttatatagt
gttcataagagaaagtatttgtaatattacaccatcttttgaaatcaaagattcttacaaagtacatcaatttaagaaagtttactctcagaacttacaa
cattcaaaagaagagagtactacacaaagtttacaccttcaagcttcttcttcgatggctttctttgcagtttcgaggtcttcgtcaaggaatatgtccc
aatggccatcccaagtgatgaagaactcagtgtcttcgtgatatttgactcggtgaacatcaccggcgggagtgaataggtaatcgccgtcaacgagatc
agctctctccttcttgctcagattccacacacttttctttccctttatgactacaaggtcatggccgaatgtgtggtgatgcgccggttccacacttccc
gctttgaatcttacaatcgccgacgtcggcgtctctctaacgatcttcatcgagcctcccggcatgatgtccactggagtgaactctctgttcttcgcca
agcagcaaattacgcaagacgcggttgtttcatcgatcttcttggttgttgaatcgccggcggttgactttccaatctgagacatctcggagagagaatt
gtcgaggaaggaagagataactgagctccatggttcggatccggacgcaggacaaggagatttgaaaggagttttgatccaagcgtcgaagatttccaag
gcggtttccggggatgttttgatgccggagaatgcgaggacattgcaattgcttattgatcgagcgttgacggcgtcttcgacggagagacaagtggctg
cgtagacgcccgggaacttgttggcgaacatcgcaactccgacgccggtgccgcagcagactaagccgcgaacttcggaagacgatgaagcggagacgcg
gcgaccgacttcagatccagcagagtagtaagaggacactccggtatcctccacgtctatgccgagagagcggaggtgtgttaccatcgcgtccttgagg
ctcgctccaaagtcatcagctccggtgatgattttcaacggttgatcgacggcagaaacagctccggccatcttttcagagctttgaaaccttaagctgt
gaccagaagaatcttaaattggaaattttcggaaaaaagagaaagacataagttacactactcttccacatttcaccctattaagttattttaacacaaa
aggaccctccaattttaaatgaaataaggtactataaattaataaataaaagttttagggtggaaacaaaattataagggaacaacatgccgacaaaaaa
aaaaaaaaaagggaacaacataaacatttttaatataaacatgcggcggagtcgtggcggcctttaaaataaaattgccgccgacgactattgtaagtca
ctcggctaagtaagaATGGCGGGAGTGGCTTGCCAGATATCTATCAGCTCATCTTCGACCTTTAGGgtgagtctctatggcgattagcttttccttcgct
ga

Border_1 (13528_AbB_U)

AGAAGGTTTTTCTCAGCACCCACCTACATCACCACAAAAAAAACCATACATGAGTATGAGTTTCAATCATCAAGTAAATTTGAGCAGAAGCTTTGAGAAA
CAAAAACCCTTTTTCATCAAACGGAAGAGATTGTTGTAGCATCCGAAGAAAACATGTAATCCCATTTCAATATGGTTCCCACGTCTATCTACAAAAGAAC
CCACTTTGCCACCAATGAATGTCCTCGAATCATATATATCCACCTGAAACAATCACGATGATTACACATGTATGTAACCAAATCAAATATATAAACAACT
AACAGAAGAGGAGTAAACACCTCGTGACCCTGATCCAGAAGCTCTACAGCAGTCGACATGCCTGCAAGTCCAGCTCCTATAATGGCTACTTTCAGCTTAG
GTCCCTTGTATGGTACAGGCTCTGGAGGAAATAACCCCTTTGGAGCTGTATTCATAACCAGACCATTTAAACAGTAAGAGATGATCAAGAATCACGATGC
TCCAGAAAATTATTAGAAGAAGAAAGCTTCTCATAACATGAAACCTTAAATCTAAATTT:ATTCCTTAA

Start of BLAST Alignment:NT 1122 / Exon 4 of 13 (Intron in 5’ UTR)
First 15 bp Perfect Match:NT 1122 / Exon 4 of 13 (Intron in 5’ UTR)
BLAST Details:
Query Sequence: 13528_AbB_U 
Length: (568 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At3g04870 68416.t00478 zeta-carotene desaturase (ZDS1) / ...  2759  1.4e-120  1
At5g26660 68418.t02884 myb family transcription factor (M...   193  0.75      1
At3g44010 68416.t04371 40S ribosomal protein S29 (RPS29B)...   178  0.9992    1
At2g19110 68415.t02116 ATPase E1-E2 type family protein /...   179  0.9997    1


>At3g04870 68416.t00478 zeta-carotene desaturase (ZDS1) / carotene
           7,8-desaturase identical to SP|Q38893 Zeta-carotene desaturase,
           chloroplast precursor (EC 1.14.99.30) (Carotene 7,8-desaturase)
           {Arabidopsis thaliana}
        Length = 4817

  Minus Strand HSPs:

 Score = 2759 (420.0 bits), Expect = 1.4e-120, P = 1.4e-120
 Identities = 559/569 (98%), Positives = 559/569 (98%), Strand = Minus / Plus

Query:   568 TTAAGGAAT-AAATTTAGATTTAAGGTTTCATGTTATGAGAAGCTTTCTTCTTCTAATAA 510
             ||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   789 TTAAGGAATCAAATTTAGATTTAAGGTTTCATGTTATGAGAAGCTTTCTTCTTCTAATAA 848

Query:   509 TTTTCTGGAGCATCGTGATTCTTGATCATCTCTTACTGTTTAAATGGTCTGGTTATGAAT 450
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   849 TTTTCTGGAGCATCGTGATTCTTGATCATCTCTTACTGTTTAAATGGTCTGGTTATGAAT 908

Query:   449 ACAGCTCCAAAGGGGTTATTTCCTCCAGAGCCTGTACCATACAAGGGACCTAAGCTGAAA 390
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   909 ACAGCTCCAAAGGGGTTATTTCCTCCAGAGCCTGTACCATACAAGGGACCTAAGCTGAAA 968

Query:   389 GTAGCCATTATAGGAGCTGGACTTGCAGGCATGTCGACTGCTGTAGAGCTTCTGGATCAG 330
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   969 GTAGCCATTATAGGAGCTGGACTTGCAGGCATGTCGACTGCTGTAGAGCTTCTGGATCAG 1028

Query:   329 GGTCACGAGGTGTTTACTCCTCTTCTGTTAGTTGTTTATATATTTGATTTGGTTACATAC 270
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1029 GGTCACGAGGTGTTTACTCCTCTTCTGTTAGTTGTTTATATATTTGATTTGGTTACATAC 1088

Query:   269 ATGTGTAATCATCGTGATTGTTTCAGGTGGATATATATGATTCGAGGACATTCATTGGTG 210
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1089 ATGTGTAATCATCGTGATTGTTTCAGGTGGATATATATGATTCGAGGACATTCATTGGTG 1148

Query:   209 GCAAAGTGGGTTCTTTTGTAGATAGACGTGGGAACCATATTGAAATGGGATTACATGTTT 150
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1149 GCAAAGTGGGTTCTTTTGTAGATAGACGTGGGAACCATATTGAAATGGGATTACATGTTT 1208

Query:   149 TCTTCGGATGCTACAACAATCTCTTCCGTTTGATGAAAAAGGGTTTTTGTTTCTCAAAGC 90
             |||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
Sbjct:  1209 TCTTCGGATGCTACAACAATCTCTTCCGTTTGATGAAAAAGG-TTTTTGTTTCTCAAAGC 1267

Query:    89 TTCTGCTCAAATTTACTTGATGATTGAAACTCATACTCATGTATGGNNNNNNNNGTGGTG 30
             ||||||||||||||||||||||||||||||||||||||||||||||        ||||||
Sbjct:  1268 TTCTGCTCAAATTTACTTGATGATTGAAACTCATACTCATGTATGGTTTTTTTTGTGGTG 1327

Query:    29 ATGTAGGTGGGTGCTGAGAAAAACCTTCT 1
             |||||||||||||||||||||||||||||
Sbjct:  1328 ATGTAGGTGGGTGCTGAGAAAAACCTTCT 1356


>At5g26660 68418.t02884 myb family transcription factor (MYB4) (MYB86) contains
           Pfam profile: PF00249 myb-like DNA-binding domain; identical to cDNA
           putative transcription factor (MYB86) mRNA, partial cds GI:3941517
        Length = 1598

  Minus Strand HSPs:

 Score = 193 (35.0 bits), Expect = 1.4, P = 0.75
 Identities = 145/236 (61%), Positives = 145/236 (61%), Strand = Minus / Plus


Border_2 (13528_LB1D)

TATGTATGTCAATTGGAGGTATGGCTCAGTTCATTATTCTCTTGCTATTGTGATTTTATCGTACTGAGATGTGCATATTATTTGTAGAGCTTGATTTTCG
GTTTCCTGTTGGAGCTCCAATCCATGGTATTCGCGCATTTTTGGTCACAAATCAACTCAAGGTATAAATCAAACTTTTAAATTGATTTATCTTTTAAATC
TTTGGATTCATATCAATGCACTTTTCCGCCGTGGAAAATCGGCAACAGTTTTCTCAAATGTTCATAGCAAAAGATTAGTAATTTCTTGCTTTATGTACCA
ATATCGAGCTTGCTAACTGCTTATTCTTGTTTTGACCGAACAGCCCTATGACAAACTAAGGAATTCACTGGCTCTTGCATTAAGCCCTGTTGTTAAAGCT
CTTGTCGACCCCGATGGAGCAATGAGAGACATTCGCAACCTTGACAGCGTAAAGAATGATAACCTGGTCTTCTTCTC

Start of BLAST Alignment:NT 1164 / Exon 4 of 13 (Intron in 5’ UTR)
First 15 bp Perfect Match:NT 1164 / Exon 4 of 13 (Intron in 5’ UTR)
BLAST Details:
Query Sequence: 13528_LB1D 
Length: (477 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At3g04870 68416.t00478 zeta-carotene desaturase (ZDS1) / ...  2331  3.4e-100  1
At4g29100 68417.t03866 ethylene-responsive family protein...   179  0.994     1


>At3g04870 68416.t00478 zeta-carotene desaturase (ZDS1) / carotene
           7,8-desaturase identical to SP|Q38893 Zeta-carotene desaturase,
           chloroplast precursor (EC 1.14.99.30) (Carotene 7,8-desaturase)
           {Arabidopsis thaliana}
        Length = 4817

  Plus Strand HSPs:

 Score = 2331 (355.8 bits), Expect = 3.4e-100, P = 3.4e-100
 Identities = 467/468 (99%), Positives = 467/468 (99%), Strand = Plus / Plus

Query:    10 CAATTGGAGGTATGGCTCAGTTCATTATTCTCTTGCTATTGTGATTTTATCGTACTGAGA 69
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1398 CAATTGGAGGTATGGCTCAGTTCATTATTCTCTTGCTATTGTGATTTTATCGTACTGAGA 1457

Query:    70 TGTGCATATTATTTGTAGAGCTTGATTTTCGGTTTCCTGTTGGAGCTCCAATCCATGGTA 129
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1458 TGTGCATATTATTTGTAGAGCTTGATTTTCGGTTTCCTGTTGGAGCTCCAATCCATGGTA 1517

Query:   130 TTCGCGCATTTTTGGTCACAAATCAACTCAAGGTATAAATCAAACTTTTAAATTGATTTA 189
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1518 TTCGCGCATTTTTGGTCACAAATCAACTCAAGGTATAAATCAAACTTTTAAATTGATTTA 1577

Query:   190 TCTTTTAAATCTTTGGATTCATATCAATGCACTTTTCCGCCGTGGAAAATCGGCAACAGT 249
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1578 TCTTTTAAATCTTTGGATTCATATCAATGCACTTTTCCGCCGTGGAAAATCGGCAACAGT 1637

Query:   250 TTTCTCAAATGTTCATAGCAAAAGATTAGTAATTTCTTGCTTTATGTACCAATATCGAGC 309
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1638 TTTCTCAAATGTTCATAGCAAAAGATTAGTAATTTCTTGCTTTATGTACCAATATCGAGC 1697

Query:   310 TTGCTAACTGCTTATTCTTGTTTTGACCGAACAGCCCTATGACAAACTAAGGAATTCACT 369
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1698 TTGCTAACTGCTTATTCTTGTTTTGACCGAACAGCCCTATGACAAACTAAGGAATTCACT 1757

Query:   370 GGCTCTTGCATTAAGCCCTGTTGTTAAAGCTCTTGTCGACCCCGATGGAGCAATGAGAGA 429
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1758 GGCTCTTGCATTAAGCCCTGTTGTTAAAGCTCTTGTCGACCCCGATGGAGCAATGAGAGA 1817

Query:   430 CATTCGCAACCTTGACAGCGTAAAGAATGATAACCTGGTCTTCTTCTC 477
             ||||||||||||||||||||||||||||||||||||| ||||||||||
Sbjct:  1818 CATTCGCAACCTTGACAGCGTAAAGAATGATAACCTGTTCTTCTTCTC 1865



>At4g29100 68417.t03866 ethylene-responsive family protein contains similarity
           to ethylene-inducible ER33 protein [Lycopersicon esculentum]
           gi|5669656|gb|AAD46413
        Length = 3604

  Minus Strand HSPs:

 Score = 179 (32.9 bits), Expect = 5.1, P = 0.994
 Identities = 137/235 (58%), Positives = 137/235 (58%), Strand = Minus / Plus