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At3g20630 SYNGENTA 14986

Genomic Sequence (from TAIR)
atgcatatgggtcaatcaaatagtcttgggtctattgggtaaatacaaactctaatgggctgtacaatattctttttgtgtatccaaactctatccttaa
tttagaatattttcaaaattaacccaacaaaaagtcggttaacgaaatttccccaaattgacaacaacttttgctttattgcaattctccccaaaacctc
aggcaataagaaaggaatttagaaggacgacgacgtttctcatcatccgtaccttccttgttaagttgtaacggagacgtgtgatatcactcaacaacac
atatctgtctctccgtcgtcagagagtttctttcgccgatttcgcaaaaaccctATGGAGCTCCTCCGATCCAACTTGTCTAGGGTTCAGATCCCTGAAC
CTACTCATCGCATCTACAAGCATGAGTGTTGCATCTCCTTCGATACTCCGgtaaagcctctctctcacttcgttttcttcttcaaattccgactactctc
gactcttctctcgttttcttcggttttgatgaattcgcaatgatgattttcagAGATCCGAAGGAGGATTGTTCGTTGATATGAATAGTTTTCTTGCTTT
CGGGAAGGATTATGTTTCTTGGAACTATGAGAAGACTGGAAACCCTGTTTATCTTCATATTAAGCAGACTAGGAAGTCTATTCCCGAGGATCGGCCTCTC
AAGAAACCGACTCTGCTCGCTATAGgtatatatacaatgatgattacaatgccgtatctatactgatttttgaacatagagtcgagagattggttttgaa
ctgttaatcaatttgcagGTGTTGATGGAGGCTTTGATAACAATGAGCCAGAGTACGAGGAGTCTTATAGCATAGTCATACTTCCGGATTTTGTTTCACT
CCCGTTTCCTTCTGTTGAGCTACCAGAGAAGgtgtttgttagcactatgttgttgattttaggtgtgttatcagagagtggtttatggaaaacacattgc
atgtatttagctttttgtgatttgttattgcagGTGAGGATTGCTGTCGATACTGTAGTGAATGCCGTTGGTGCTGAGCGGAAAGAGCAAGTTGCAGCTT
GGACAGCTGAGAAAAAGTTAATTAGTGAACATGCATTGACACTGCAGCAAATCAAGAGTGGCATTGTCATTCCTCCCTCTGGTTGGAAATGTTCTAAGTG
TGATAAGACGGAGAATCTCTGGCTTAATCTTACCGATGGAATGATTCTCTGTGGAAGAAAAAACTGGGATGGAACTGGTGGAAACAACCATGCTGTTGAG
CACTACAAAGAGACAGCCTACCCTCTTGCTGTAAAGCTTGGAACTATCACTGCTGACTTAGAAGCAGCAGgtaaagttgtcttgtttccttattcgtaga
accagagattagtcattaaacttcacttttgtgtatggctaatatcagttgatagttgtctctttggggtggataagggggacttcctgcatcactttgt
tttttttttttaatgttgatgcatgtggattatcttgttctttggatgggttttgatttcttcagcacttaatcattgcaaattttttggcagctcagAT
GTTTATTCCTATCCGGAAGATGACAGTGTTTTGGACCCGCTCCTGGCTGAGCATCTGGCCCATTTTGGAATTGACTTCTCGTCGATGCAGAAGgttagta
atgatggttcatggttctgttaaagtataaaagtgaagagtggatcttgtctgacactttatattgttgtttgtctgtggtaatgaatttcgtgtactgc
atttttattagatactcaagcttaggtattcccataatgatatggtttgttaagatacccatcctgggttgcattggcaagtgctggttgtactagaaag
cttatgtcttgtgttcatttctttcttcagtatcaattgccacaaaacaactgtagaaacatggaatatgtctgagactaaatgtttctttaccaagagc
aagtagactcacatcagtgttattgcgtacttttttataattgcgattaaaaagacgcagtgcacttggagaatgtaatttcaggtcgttttgagacaat
aattcccattcacgcgttcagtctgtttacttgtaattgttgcgtcacatgtaaacatttacaatagtaaactgtattgtgtcttttttcctcatttttc
cttgctccatgtactgcttaagttaagaggcttcatttcccaaagcataggttttatagtcacctgtatttgttttcttgtttgtactgccttgatcatc
atctttgctaaagatatcccatgctggttttaatctgaataatgttaactttcttgaaatgtttgctacaattttcacagACTGAAATGACAACTGCTGA
AAGAGAACTTGATCAAAATACAAATTTTGATTGGAACCGTATACAAGAGAGTGGAAAAGAATTGGTGCCAGTTTTTGGACCTGGATATACCGGACTTGTC
AATCTTGGGAACAGgtactctaatggaaaaattgtcagggatttcgtttccttgttgatgtcttatgcccatttcttttcttttgccagTTGCTACTTGG
CAGCTACGATGCAGATTGTTTTCTCTACCCATTCATTTATTTCAAGgtagatgccattgcccatatttctttttcacttatataacttaaatcaaaacaa
agcttttgctctttgattcaactgtagtaacttttggttctgagattgttgcagATACTTTTCACATCAGAGCTTAAAGATGGCTTTTGAGATGGCTCCT
GCTGATCCAACTTTGGACCTCAATATGCAATTgtgagttattgttagaaaaaatgttactaagtttatgctctggactttctgaaatcatgtgattgaaa
tcttggcatcgttatgatgaaagggttttaagattatatttgtgcatactctgtatctgcatgcacgtgctttagtttctgtttttctctccgtttggtt
tgatatgctgtgcactttttctaactaatctgatttccatgaggaataattgcatggtaatttgcatatttatttcatactccttgcactgttggttgta
gAACAAAGCTTGGACACGGCTTACTATCTGGGAAGTACTCAATGCCTGCAACTCAGgtataactatagtaggaaatatttttgcttgtatgcatatatat
atgtatatgatctaaaccaccttttagagcctcagtacattgatttcgcctttcttctgattgcagAAGGATGCTACAACTGGAGATCCTgtaagatttt
tatttgaagtcctgtgtttaacttttgaataaatatatgtatgcggatggccacacttttttttttgcataccccacttgtgttgatttctgatgact
ttacatcttgtttcgttttttaatcgataatagAGACAAGAAGGAATACCTCCTCGCATGTTCAAAAACGTAATTGCCGCGAGCCACGCAGAATTCTCTT
CTATGAGACAGCAGgtgaaaaccatgtcttttttaatgttcggagttttgtttattttttaacttgtattggaacgatactccttttcggcatcagttaa
cgaaattattttatgttttcatactaactagcaccaatctgtcttgtagGATGCTCTCGACTTTTTCCTCCATCTGGTAGGCAAGGTTGAGCGTGCTAGC
AACACGACACCAGATTTAGACCCCTCGAGGAGCTTCAAGTTTGGAATAGAAGAGAAGATCCTTTGTCCGTCTGGAAAGGTTGGATATAATAAGAGGGAAG
ACTGTATTCTTTCTTTGAACATTCCGCTACATGAGGCAACTAATAAAGgtatatcctgttcgtctgtctatgttcctcttcctttatcgtttggcgtgtt
tcttaattttgcttaatgtttggtgatcaagATGAATTAGAAGCCTTTCACAAGCAAAAAGCAGGAAAAGGATTGGAAGAGAATGATATgtgagtcaatg
gacttaagtttaaatctgtttcatagtttcactgcttatctaagatttttttgtttattttcagGAGGTCAAGTGATGAAATAGTACGCCCACGAGTTCC
TTTAGAAGCCTGTCTAGCAAATTTTGCATCATCAGAGCCGATTGAAGACTACTATAGCTCTGCTTTGAAGGGAATGACAACAGCTATCAAgtaagatatt
gttttttaccgagtgaattcttttgcaaccaagattagaatgaaagcaaggcttgacttttggttcccattttatgcagGACAACTGGTTTGACATCTTT
CCCAGATTATTTGGTCTTGCACATGCGGAAATTTGTTATGGAGGAAGGCTGGGTGCCAAAGAAACTTGgtctctttctttctgaaacaatgatccttata
atgttcccctattgtccctgtatataaatacttattatcctttcttgatttactgtggcagATGTATACATTGATGTTCCGGATGTTATTGATATCAGCC
ACATGCGTAGCAAAGGACTCCAACCTGGGGAAGAACTGTTGCCAGATGGCGgtatgttgttatatttatcacaggagtggtgtgttcataattcacataa
ataatgattgattgactccatttattgattgtttcaaactcgtggtatcttttctggtgtcatgactactagcctttggaattagcactcagtttcctaa
tcattaaaaagtatcagcatcccttaaacggtttctttatggcactcagtctccttataattctgttggctattggatttttctgtatacagTTCCAGAA
GAAGTGATGGAATCAGCGCAGCCCGTGGCAAATGAGGAGATAGTTGCACAGCTAGTCTCAATGGGATTTAGCCAGCTTCACTGCCAGAAAGCTGCCATAA
ATACTTCCAATGCTGGGGTTGAAGAGGCAATGAACTGGTTACTTTCTCACATGGATGATCCAGgtaattgaaaatagttccacaagtctttagttgaggc
tcaactaaattacgactcgagaccgatttctaatatgatgaggcattatgtttcatagACATCGATGCACCAATCTCCCACCAGACATCTGACATTGATC
AATCAAGCGTTGATACCTTACTCTCCTTTGGTTTTGCTGAAGATGTTGCTCGGAAGGCACTAAAAGCCTCGgtataacacttttgagcaaattagttaca
tttctgcgaggctgttttttctttactcattactgaaatcggttttgttttttttgtctttaatcagGGAGGAGACATTGAGAAAGCAACAGACTGGGTA
TTCAACAACCCTAATGCATCTGTTTCAGACATGGATGTATCCTCTAGCAATTCAGCGCAGACTCCGGCTCAAAGTGGATTACCAGATGGAGGAGGGAgtt
agtttcttaaaccctcttttgagtctttctatctcattttgcaaaacaaatgttagtgtttcgttttttccacaaagcagtatgaataaaactctaactc
aagattgatcttaaatgcagAATACAAGCTGTTTGGAATAGTAAGTCACATGGGAACATCAGTGCACTGTGGTCATTACGTGGCTCACATATTGAAAGAA
GGCCGCTGGGTAATTTTCAATGACGACAAAGTTGGTATCTCGACTGATCCTCCTAAAGACATGGGTTATGTCTACTTCTTTCAGCGGCTTGATTGAttcg
atggagccagttacagagacaaaaactaatccttttttttttaaatagaaacttttaaggtcgatagggtttttaacatttggattgaggtgacccatgt
gactagtttcggttccaaaatcttacatgtggattagtaaatttggttcacttgatgattttatctgtggaacttcgtcacaaattttaagacccgttta
gaagctaaacatcattcgctgtctttttaatttgcctcctcagttctcaactactttcagttttttttttttttcttttggataaaagttttgagctttt
ccttcagttttggcatcattggtgcttagatttatgaaaaatgaagatataaaaggggaatcataagtgtttttttttacattagcagaaaaactgtt
gatt

Border_1 (14986_RBU)

AACAGCTATGACCATGATTACGCCAAGCTCGAAATTAACCCTCACTAAAGGGAACAAAAGCTGGAGCTCGCTTGAGCTTGGATCAGATTGTCGTTTCCCG
CCTTCAGTTTAAACTATCAGTGTGGCCATCCGCATACATATATTTATTCAAAAGTTAAACACAGGACTTCAAATAAAAATCTTACAGGATCTCCAGTTGT
AGCATCCTTCTGCAATCAGAAGAAAGGCGAAATCAATGTACTGAGGCTCTAAAAG

Start of BLAST Alignment:NT 3011 / Intron 10 of 19
First 15 bp Perfect Match:NT 3003 / Intron 10 of 19
BLAST Details:
Query Sequence: 14986_RBU 
Length: (255 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At3g20630 68416.t02332 ubiquitin-specific protease 14, pu...   689  2.4e-25   1
At4g37040 68417.t04818 metallopeptidase M24 family protei...   153  0.36      1


>At3g20630 68416.t02332 ubiquitin-specific protease 14, putative (UBP14)
           similar to ubiquitin-specific protease 14 GI:11993473 [Arabidopsis
           thaliana]
        Length = 5340

  Minus Strand HSPs:

 Score = 689 (109.4 bits), Expect = 2.4e-25, P = 2.4e-25
 Identities = 141/145 (97%), Positives = 141/145 (97%), Strand = Minus / Plus

Query:   255 CTTTTAGAGCCTCAGTACATTGATTTCGCCTTTCTTCTGATTGCAGAAGGATGCTACAAC 196
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2867 CTTTTAGAGCCTCAGTACATTGATTTCGCCTTTCTTCTGATTGCAGAAGGATGCTACAAC 2926

Query:   195 TGGAGATCCTGTAAGATTTTTATTTGAAGTCCTGTGTTTAACTTTTGAATAAATATATGT 136
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2927 TGGAGATCCTGTAAGATTTTTATTTGAAGTCCTGTGTTTAACTTTTGAATAAATATATGT 2986

Query:   135 ATGCGGATGGCCACACTGATAGTTT 111
             |||||||||||||||||  |  |||
Sbjct:  2987 ATGCGGATGGCCACACTTTTTTTTT 3011


>At4g37040 68417.t04818 metallopeptidase M24 family protein similar to
           SP|O33343 Methionine aminopeptidase (EC 3.4.11.18) (Peptidase M)
           {Mycobacterium tuberculosis}; contains Pfam profile PF00557:
           metallopeptidase family M24
        Length = 2219

  Plus Strand HSPs:

 Score = 153 (29.0 bits), Expect = 0.44, P = 0.36
 Identities = 109/183 (59%), Positives = 109/183 (59%), Strand = Plus / Plus


Border_2 (14986_LBD)

TTCAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTACACCACAATATAAAAGGGGAATCA
TAAGTGTTTTTTTTTACATTAGCAGAAAAACTGTTGATTAAAATATTTAATCTCGGGATACACTGAAATTGGAGATCTTTTAATTAGTTTTTAAGATCAA
AAAGTGGGCTAATTTGTTATATAGTTAACACTGGCCATCATTATAATCTCGAAGA

Start of BLAST Alignment:NT > 327 / Downstream
First 15 bp Perfect Match:NT > 351 / Downstream
BLAST Details:
Query Sequence: 14986_LBD Border #2 
Length: (255 letters) 
Database: TIGR AGI BAC Sequences (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

K10D20 60546                                                   798  7.2e-30   1
F16N3 51472                                                    170  0.16      1
F4P12 67286                                                    167  0.21      1
F11K13 68394                                                   164  0.27      1


K10D20 60546
        Length = 55,481

  Minus Strand HSPs:

 Score = 798 (125.8 bits), Expect = 7.2e-30, P = 7.2e-30
 Identities = 174/195 (89%), Positives = 174/195 (89%), Strand = Minus / Plus

Query:   255 TCTTCGAGATTATAATGATGGCCAGTGTTAACTATATAACAAATTAGCCCACTTTTTGAT 196
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 51945 TCTTCGAGATTATAATGATGGCCAGTGTTAACTATATAACAAATTAGCCCACTTTTTGAT 52004

Query:   195 CTTAAAAACTAATTAAAAGATCTCCAATTTCAGTGTATCCCGAGATTAAATATTTTAATC 136
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 52005 CTTAAAAACTAATTAAAAGATCTCCAATTTCAGTGTATCCCGAGATTAAATATTTTAATC 52064

Query:   135 AACAGTTTTTCTGCTAATGTNNNNNNNNNCACTTATGATTCCCCTTTTATATTGTGGTGT 76
             ||||||||||||||||||||         |||||||||||||||||||||||  |  | |
Sbjct: 52065 AACAGTTTTTCTGCTAATGTAAAAAAAAACACTTATGATTCCCCTTTTATATCTTCATTT 52124

Query:    75 AA-ACAAATTGACGC 62
                | ||||  | ||
Sbjct: 52125 TTCATAAATCTAAGC 52139


F16N3 51472
        Length = 171,769

  Plus Strand HSPs:

 Score = 170 (31.6 bits), Expect = 0.17, P = 0.16
 Identities = 72/103 (69%), Positives = 72/103 (69%), Strand = Plus / Plus