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At5g24314 SYNGENTA 19390

Genomic Sequence (from TAIR)

CATGATCATAGCTAAGAGAACACAACATATTAAGAGATGATTCCTAGAAGCCATtttctcatccggtgatcgatagagttaatttctttgcttatcaact
atgttgaggttccatcttgtaatgtcttatgtatatacatagctcacaaagtcacaatagagggttcccactaactattattagattgattgatttctga
aaaatattatttttgtgtaccaagtaaaattatatgattttagatttgttgttagttttgatttgttttagattttttttttgtgcaagttgaattatta
ttgtatagataaaatgacgagaaaaaaaaatgcccggttcggttaggaatgatttgtccaaatacgaacacaaccaaagataaaccattaaaccgaaccg
gagaagcttacatataaaataggatccggttctatctattcactg►tgtatcaATGGC◄TTCCTTCACCTGT►TCTTCTCCATCTTCGATTTTACCTgta
aaaatccctcctttgcttctctcataatcatcctttgatgtcaatgtgatttctcattttcaattattgtatattcagATTATTGATACGAGAAGTGGGA
ATTTGCGATGCACATTTCAGTCTCAGgttttgaatggttgatatatatacgaatttgtgggtttttgggggagagttttgagtgaatctggttgattttt
tcagGTTTCTTGTGGGATTCAGAGAGATGATAATGGACGCCGTGTTTGGCGGAGGAGAACATTGgtaagtactaaacttgatattttggtgattttccag
tttcatgtttatgacataagtcgaatcaggaatcaaaataagggtttttctttgcttggagacgacatttctaaaaagttgcaatttttagtaggatcac
agattagctttaactcactgatgaggcatgactattaactctagttagtctttacttagtcatagtaattgatatgatgcatagaaggagagatggagat
tgtcttctctgagtttttgcgatttctagtttacccttcttgattccaaattagcttacacacctgtttcaaaagtgtctttgttgaactatgctcattt
cagacgctagctttagttcttatactgttgatgaacttaaagaaacatggctttttacctctagttagtgtttacttatatatattcaagtaaccatggt
cttatctctatggcacatgcaagaaattggagattgtcttatctgagatactgggatttttaggtgggagttacacattacataaaatattagtgtgatc
tttttacttgtactatattctacaaactatcagtttctgttgtttgatcctatcaggttcatgaggttttgagatttcaataattaccaaagccagaaca
atgcaaaatctttgtttatatttgtcccgcttttaatctctctatgtcgttctcctgttttgtctgtagACGAAGAAGGACGATATGTTGCGTTACAAAA
TGCAAAGAGTTCCATTTGTGGAAGAACAAGTAAGGAAGATAAGAGAAGTTGGGAAGGTAATGACAATGGACATAGAGCAGCTTCTTTTGAGGGAAGACAA
TCGGTTTGAATTTGTCAATAGCGTAGCAGCTGAAGCAACAGAGTACGTGGACAAGAACAGAGACGAATATGGAGGTTCCAAAAAAGCCATCTTTCATGTT
CTAAGCAACCGTGTGAACGATCTCGGCTTTGATCGCCCTGAGGCTTATGTAGAAGCTGATCCTTACAAACCCGGTCCTGGCTATTTGTTGGAGTACTACA
CTTGAtatattataacaaaaagtgtcaatgtactttacagcttttgttcttgtattaccaaaaccaaatcaatgcattcacagctttgttgttttcttgg
ccaaatttcattttatttatttagatttactagatgaagacgaaagtctaatgacaagagtttagtaaaagtaacggtctagtgatttgtggtgaaagga
ggtcgtcgtgtcggtggaggaggaggaggaggtacatatggcattggtcgttttgggattggtcttggagaccagtccccatagtcttctttgttataga
cagttggttcctttgtcacaacctcatcaacctttcaaaaccaaactcatacactattaaaaaaggaagacacttttcgtaatacttcatttttgctata
gggcacgtacttgtgttcctggaattgtagggtcggatggcttgggaggtcttagatgcggcccagcgtagtccatctccagatcgattcttcttccatt
cat

Border_1 (19390_LB1D)


CGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTGCCATTGATACACAGTGAATAGATAGAACCGGATCCTATTTTATATGTAAGCTTCTCCGG
TTCGGTTTAATGGTTTATCTTTGGTTGTGTTCGTATTTGGACAAATCATCCCTAACCGAACCGGGCATTTTTTTTTCTCGTCATTTTATCTATACAATAA
TAATTCAACTTGCACAAAAAAAAAATCTAAAACAAATCAAAACTAACAACAAATCTAAAATCATATAATTTTACTTGGTACACAAAAATAATATTTTTCA
GAAATCAATCAAT

Start of BLAST Alignment:	NT 5 / Exon 1 of 4
First 15 bp Perfect Match:	NT 5 / Exon 1 of 4

BLAST Details:
Query Sequence: 19390_LB1D 
Length: (313 letters) 
Database: TIGR AGI BAC Sequences (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

MOP9 67667                                                     957  4.5e-37   1
F2J10 51777                                                    206  0.0038    1
MVP7 67920                                                     190  0.020     1
T9E8 67115                                                     179  0.061     1


MOP9 67667
        Length = 94,194

  Minus Strand HSPs:

 Score = 957 (149.6 bits), Expect = 4.5e-37, P = 4.5e-37
 Identities = 215/273 (78%), Positives = 215/273 (78%), Strand = Minus / Plus

Query:   313 ATTGATTGATTTCTGAAAAATATTATTTTTGTGTACCAAGTAAAATTATANNNNNNNNNN 254
             ||||||||||||||||||||||||||||||||||||||||||||||||||          
Sbjct: 65046 ATTGATTGATTTCTGAAAAATATTATTTTTGTGTACCAAGTAAAATTATATGATTTTAGA 65105

Query:   253 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGCAAGTTGAATTATTATTGT 194
                                                   ||||||||||||||||||||||
Sbjct: 65106 TTTGTTGTTAGTTTTGATTTGTTTTAGATTTTTTTTTTGTGCAAGTTGAATTATTATTGT 65165

Query:   193 ATAGATAAAATGACGAGNNNNNNNNNTGCCCGGTTCGGTTAGGGATGATTTGTCCAAATA 134
             |||||||||||||||||         ||||||||||||||||| ||||||||||||||||
Sbjct: 65166 ATAGATAAAATGACGAGAAAAAAAAATGCCCGGTTCGGTTAGGAATGATTTGTCCAAATA 65225

Query:   133 CGAACACAACCAAAGATAAACCATTAAACCGAACCGGAGAAGCTTACATATAAAATAGGA 74
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 65226 CGAACACAACCAAAGATAAACCATTAAACCGAACCGGAGAAGCTTACATATAAAATAGGA 65285

Query:    73 TCCGGTTCTATCTATTCACTGTGTATCAATGGC 41
             |||||||||||||||||||||||||||||||||
Sbjct: 65286 TCCGGTTCTATCTATTCACTGTGTATCAATGGC 65318


F2J10 51777
        Length = 91,720

  Plus Strand HSPs:

 Score = 206 (37.0 bits), Expect = 0.0038, P = 0.0038
 Identities = 86/135 (63%), Positives = 86/135 (63%), Strand = Plus / Plus

Border_2 (19390_AbB_U)


TATAAGAACCCTAATTCCCTTATCTGGGAACTACTCACACATTATTATAGAGAGAGATAGATTTGTAGAGAGAGACTGGTGATTTCAGCGGGCATGCCTG
CAGGTCGACTCTAGAGGATCCTAGAACGCGTGATCTCAGATCTCGGTGACGGGCAGGACCGGACGGGGCGGTACCGGCAGGCTGAAGTCCAGCTGCCAGA
AACCCACGTCATGCCAGTTCCCGTGCTTGAAGCCGGCCGCCCGCAGCATGCCGCGGGGGGCATATCCGAGCGCCTCGTGCATGCGCACGCTCGGGTCGTT
GGGCAGCCCGATGACAGCGACCACGCTCTTGAAGCCCTGTGCCTCCAGGGACTTCAGCAGGTGGGTGTAGAGCGTGGAGCCCAGTCCCGTCCGCTGGTGG
CGGGGGGAGACGTACACGGTCGACTCGGCCGTCCAGTCGTAGGCGTTGCGTGCCTTCCAGGGGCCCGCGTAGGCGATGCCGGCGACCTCGCCGTCCACCT
CGGCGACGAGCCAGGGATAGCGCTCCCGCAGACGGACGAGGTCGTCCGTCCACTCCTGCGGTTCCTGCGGCTCGGTACGGAAGTTGACCGTGCTTGTCTC
GATGTAGTGGTTGACGATGGTGCAGACCGCCGGCATGTCCGCCTCGGTGGCACGGCGGATGTCGGCCGGGCGTCGTTCTGGGCTCATGGATCCACGTGTG
GAAGATATGAATTTTTTTGAGAAACTAGATAAGATTAATGAATATCGGTGTTTTGGTTTTTTCTTGTGGCCGTCTTTGTTTATATTGAGATTTTTCAAAT
CAGTGCGCAAGACGTGACGTAAGTATCCGAGTCAGTTTTTATTTTTCTACTAATTTGGTCGTTTATTTCGGCGTGTAGGACATGGCAACCGGGCCTGAAT
TTCGCGGGTATTCTGTTTCTATTCCAACTTTTTCTTGATCCGCAGCCATTAACGACTTTTGAATAGATACGCTGACACGCCAAGCCTCGCTAGTCAAAAG
TGTACCAAACAACGCTTTACAGCAAGAACGGAATGCGCGTGACGCTCGCGGTGACGCCATTTCGCCTTTTCAGAAATGGATAAATAGCCTTGCTTCCTAT
TATATCTTCTCCATCTTCGATTTTACCTGTAAAAATCCCTCCTTTGATTCTCTCATAATCATCCTTTGATGTCAATGTGATTTCTCATTTTCGATTATTG
TATATTCAGATTATTGATACGAGAAGTGGGAATTTGCGATGCACATTTCAGTCTCAGGTTTTGAATGGTTGATATATATACGAATTTGTGGGTTTTTGGG
GGAGAGTTTTGAGTGAATCTGGTTGATTTTTTCAGGTTTCTTGTGGGATTCAGAGAGATGATAATGGACGCCGTGTTTGGCG

Start of BLAST Alignment:	NT < 7 / 5’ UTR
First 15 bp Perfect Match:	NT 18 / Exon 1 of 4

BLAST Details:
Query Sequence: 19390_AbB_U 
Length: (1382 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At5g24314 68418.t02597 expressed protein                      1395  1.8e-58   1
At4g26910 68417.t03598 2-oxoacid dehydrogenase family pro...   193  0.9994    1
At3g06130 68416.t00636 heavy-metal-associated domain-cont...   191  0.9998    1
At4g30590 68417.t04026 plastocyanin-like domain-containin...   188  0.9999    1


>At5g24314 68418.t02597 expressed protein
        Length = 1449

  Plus Strand HSPs:

 Score = 1395 (215.4 bits), Expect = 1.8e-58, P = 1.8e-58
 Identities = 293/306 (95%), Positives = 293/306 (95%), Strand = Plus / Plus

Query:  1077 TGGATAAATAGCCTTGCTTCCTATTATATCTTCTCCATCTTCGATTTTACCTGTAAAAAT 1136
             || || ||| || || ||||   |  | ||||||||||||||||||||||||||||||||
Sbjct:     2 TGTATCAATGGC-TTCCTTCACCTG-T-TCTTCTCCATCTTCGATTTTACCTGTAAAAAT 58

Query:  1137 CCCTCCTTTGATTCTCTCATAATCATCCTTTGATGTCAATGTGATTTCTCATTTTCGATT 1196
             |||||||||| ||||||||||||||||||||||||||||||||||||||||||||| |||
Sbjct:    59 CCCTCCTTTGCTTCTCTCATAATCATCCTTTGATGTCAATGTGATTTCTCATTTTCAATT 118

Query:  1197 ATTGTATATTCAGATTATTGATACGAGAAGTGGGAATTTGCGATGCACATTTCAGTCTCA 1256
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   119 ATTGTATATTCAGATTATTGATACGAGAAGTGGGAATTTGCGATGCACATTTCAGTCTCA 178

Query:  1257 GGTTTTGAATGGTTGATATATATACGAATTTGTGGGTTTTTGGGGGAGAGTTTTGAGTGA 1316
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   179 GGTTTTGAATGGTTGATATATATACGAATTTGTGGGTTTTTGGGGGAGAGTTTTGAGTGA 238

Query:  1317 ATCTGGTTGATTTTTTCAGGTTTCTTGTGGGATTCAGAGAGATGATAATGGACGCCGTGT 1376
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   239 ATCTGGTTGATTTTTTCAGGTTTCTTGTGGGATTCAGAGAGATGATAATGGACGCCGTGT 298

Query:  1377 TTGGCG 1382
             ||||||
Sbjct:   299 TTGGCG 304


>At4g26910 68417.t03598 2-oxoacid dehydrogenase family protein similar to
           SP|P36957 Dihydrolipoamide succinyltransferase component of
           2-oxoglutarate dehydrogenase complex, mitochondrial precursor (EC
           2.3.1.61) {Homo sapiens}; contains Pfam profiles PF00198: 2-oxo acid
           dehydrogenases acyltransferase (catalytic domain), PF00364:
           Biotin-requiring enzyme
        Length = 3404

  Plus Strand HSPs:

 Score = 193 (35.0 bits), Expect = 7.4, P = 0.9994
 Identities = 117/197 (59%), Positives = 117/197 (59%), Strand = Plus / Plus