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At5g20920 SYNGENTA 19520

Genomic Sequence (from TAIR)
atttaataagaaataaatcttaatttgaaaaaaaaaaaattgatttgataaaatttcaattgattaattaattaagacaaataaaataataaatgagaat
ctcagtgtcaattaatagataaaattaaaatgtaacatgcgttaagaaaaagaaaaaagtaaaaatgtaatattaaggtttaggcaaaatgaaatattta
cagagaccttttactattgaattaaaaaaaaaagttacaattcgagatggactatttttttggaggagcaagggttaactcttatctcagattaaaac
aacccctgcgccgcttactctctctcagattcctcccgaagcaacctcgtttactctctacgccaatttcagcgcttagacatccctttgtgagttttc
ttcgattccttttcgaattcttctggtcgatctcagagtaaatcgtgtttttggaagaattgagtataaacagattggtaaacctagctgttccgtttct
gatttgaagccttgtcgtacattttatttgggtattttttttattgggaatcgaaaagttgttctgaatctcgttatggttttgttgcgttccgaattac
ctctctgatttagaaaccctaatttcggacttgcctatgctcatcgttgttacagattatgttgattctaatatgtatatgtttggtttctgaggtctta
ttatcattgtgtgttttcgttttgtatctcagttgcaatcATGGCTGATGAAATCAATGAGATAAGGGAAGAGCAAGAAgtaagtttgttttttttctct
gtttcttaatgatagggctcaagttagccatatagatttttttttttgttttgtttgatgaactcatgattgaatggtttttatgttttggttcatcctt
ttacagCAGCTCGCACCCTTTGATCCTTCCAAGAAGAAAAAGAAGAAGAAGGTTGTGATTCAGGAACCTGTCGAGGACTTAGCAGAGTCTTCACAGACTG
AGAAATCTGATTCATTGCCTGgtatgtatgatgaagagtcaatgtgatgtgaaacattttttttgtttcaatggtcttttgtgttactttgtctaattcc
ttgtttttgcagTTAATGATGGTCTTGAGAGTTCATTTACTGGAATGAAGAAAAAGAAGAAGAAGCCAgtgagtattgttttacgttttgtgcctagtat
tttttttatgtcattaaacttgtgtgtttgatgattctgtgactcacatttgttcttgtttgatatttagACTGAATCAAGCTTATTGAACAACGAAAGT
GTTGATGCTGGGGAAGATTTGGATGgtacttttaatatcctttttttttgtttgctaatataccttttctcttggcggggtttttatcctgtttgaatct
tactgagaatatattgcagAGATTGCTAATGACGAGCAAGAGGGGGAAGAAGGAATAGTTCTACAGCAACGTTACCCCTGGGAGGGAAGTGAGAGGGATT
ACATATATGACGAGgtgactatattttcgatgatttcttttgttttaatccttcataaatatcttgctcccttgtttttttgctgacgggagttatatat
gcagCTTCTTGGTAGAGTCTTTAACATTCTCCGTGAAAATAATCCGGAGCTTGCTGGAGATAGGCGTCGTACAGTTATGAGGCCTCCTCAAGTTCTTCGT
GAGGGGACAAAGAAGACTGTCTTTGTCAACTTCATGGACCTTTGCAAGACgtatgagctaactctttatcttcctcttcaaactttttctgtgtaaccat
aagtaatattaccatttaattttgatgacttttcgatgacatgtcttatctgcatataaaaaatcagGATGCATCGTCAACCAGATCATGTTATGCAATA
CTTGCTTGCTGAGTTGGGTACTAGTGGTTCGCTTGATGGGCAGCAAAGGTTGGTTGTTAAGGGAAGGTTTGCACCTAAGAATTTTGAAGGAATTTTGCGG
CGATATATCAgtaagtaactttcttcttagtctcaaaatttaagttgccataaaagtatatcagtttggagttgttaacctcttgttttattatttctca
gCTGACTACGTCATTTGCCTTGGTTGCAAGAGCCCAGACACCATTCTCTCCAAGGAGAACCGTCTCTTCTTTCTGAGATGTGAAAAGgtataagttaatc
taattagtcctgatcttgatatgcattcctttgtttctgttttacagttttactttctgcgcaacaaagtaataaagtattttgtgtgtttgaatttgct
aatgtgattaacgagtgggctacatggtttttgcagTGTGGATCTCAACGATCTGTGGCTCCGATCAAAACAGGGTTTGTTGCTCGTGTTAGTCGCAGGA
AGACTTGAgaaattagaaggtgaagtgaccttggtatggagtttggagctattctactgcttctgtatgagtttatgagttgaagaaatacttgtcttgt
tttttttattttgttttggaatatgattatgacttgacttttaaaatgggataggatcaaaaccttttactctgtcaggttcatgtggtcaccttgaagg
ttgatttagtaaatccatggacttcttttttgtgttaagattattcttagttcaaaattaatagactaatgatattaacgtccacaggcattgcgttcaa
catctcaaattaaagcgtggaaggctcagaaagtccaatatacactatgtttatctacagttacaatcatactacaaaaaacaaataatgtatacggttt
ggtctaatatagccgcatacgatttagtatttaccaacaaaaaattggtctcaaaccaaaccgaacaattggtaattaacaattgttcttttggtcttga
acc

Border_1 (19520_LB2U)

GCCTTTTCAGAAATGGATAAATAGCCTTGCTTCCTATTATATCTTCCCAAATTACCAATACATTACACTAGCATCTGAATTTCATAACCAATCTCGATAC
ACCAAATCGAATTCAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAARCGTCCGCAATTTGTTTAATGTGTTACT
TTTTTTTTTAATTCAATAGTAAAAGGTCTCTGTAAATATTTCATTTTGCCTAAACCTTAATATTACATTTTTACTTTTTTCTTTTTCTTAACGCATATTA
CATTTTAATTTTATCTATTAATTGACACTGAGATTCTCATTTATTATTTTATTTGTCTTAATTAATTAATCAATTGAAAATTTATCAATTTTTTTTTTTT
TTTATCAAATTAAGATTTCTTTCTTATTAAATTCTGTGAATAGTAAATTATATAGAAATGTTATTAAATATAAATGAAACTAGTAAAGAAAGAAATCAGA
ATATGTTTAGTGTCAAAATTAATAGTCTAGCATATCTTATTTTACTATAAATTACTAAATTATATTATTTTATTATTTAATATATTAAGTATTTTAGTTT
GTTAGATATTCAGTAACAAAATTTCCGCAAATGCATTAATTTTCAACCGCTAA

Start of BLAST Alignment:< 495 / Upstream
First 15 bp Perfect Match:< 514 / Upstream
BLAST Details:
Query Sequence: 19520_LB2U Border #1 
Length: (653 letters) 
Database: TIGR AGI BAC Sequences (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

F22D1 68077                                                   1313  3.9e-53   1
T9L6 51781                                                     280  6.0e-05   1
F9L11 51903                                                    280  6.0e-05   1
K11J14 60547                                                   258  0.0015    1


F22D1 68077
        Length = 96,102

  Plus Strand HSPs:

 Score = 1313 (203.1 bits), Expect = 3.9e-53, P = 3.9e-53
 Identities = 323/464 (69%), Positives = 323/464 (69%), Strand = Plus / Plus

Query:   190 AATGTGTTACNNNNNNNNNNAATTCAATAGTAAAAGGTCTCTGTAAATATTTCATTTTGC 249
             ||| ||| ||          ||||||||||||||||||||||||||||||||||||||||
Sbjct: 40849 AAT-TGTAACTTTTTTTTTTAATTCAATAGTAAAAGGTCTCTGTAAATATTTCATTTTGC 40907

Query:   250 CTAAACCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 309
             |||||||                                                     
Sbjct: 40908 CTAAACCTTAATATTACATTTTTACTTTTTTCTTTTTCTTAACGCATGTTACATTTTAAT 40967

Query:   310 NNNNTCTATTAATTGACACTGAGATTCTCATTTATTATTTTATTTGTCTTAATTAATTAA 369
                 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 40968 TTTATCTATTAATTGACACTGAGATTCTCATTTATTATTTTATTTGTCTTAATTAATTAA 41027

Query:   370 TCAATTGAAAATTTATCAANNNNNNNNNNNNNNNATCAAATTAAGATTTCTTTCTTATTA 429
             |||||||||| ||||||||                |||||||||||||| ||||||||||
Sbjct: 41028 TCAATTGAAATTTTATCAAATCAATTTTTTTTTTTTCAAATTAAGATTTATTTCTTATTA 41087

Query:   430 AATTCTGTGAATAGTAAATTATATAGAAATGTTATTAAATATAAATGAAACTAGTAAAGA 489
             ||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
Sbjct: 41088 AATTCTGTGAATAGTAAATTATATAAAAATGTTATTAAATATAAATGAAACTAGTAAAGA 41147

Query:   490 AAGAAATCAGAATATGTTTAGTGTCAAAATTAATAGTCTAGCATATCTTATTTTANNNNN 549
             ||||||||||||||| |||||||||||||||||||||||||||||||||||||||     
Sbjct: 41148 AAGAAATCAGAATATATTTAGTGTCAAAATTAATAGTCTAGCATATCTTATTTTACTATA 41207

Query:   550 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTTGTTAGATAT 609
                                                            |||||||||||||
Sbjct: 41208 AATTACTAAATTATATTATTTTATTATTTAATATATTAAGTATTTTAGTTTGTTAGATAT 41267

Query:   610 TCAGTAACAAAATTTCCGCAAATGCATTAATTTTCAACCGCTAA 653
             ||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 41268 TCAGTAACAAAATTTCCGCAAATGCATTAATTTTCAACCGCTAA 41311


T9L6 51781
        Length = 90,632

  Plus Strand HSPs:

 Score = 280 (48.1 bits), Expect = 6.0e-05, P = 6.0e-05
 Identities = 132/215 (61%), Positives = 132/215 (61%), Strand = Plus / Plus


Border_2 (19520_LB1D)

GCCTTTTCAGAAATGGATAAATAGCCTTGCTTCCTATTATATCTTCCCAAATTACCAATACATTACACTAGCATCTGAATTTCATAACCAATCTCGATAC
ACCAAATCGAATTCAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTACACCACAATATAT
CCTGCGCCGCTTACTCTCTCTCAGATTCCTCCCGAAGCAACCTCGTTTACTCTCTACGCCAGTTTCAGCGCTTAGACATCCCTTTGTGAGTTTTCTTCGA
TTCCTTTTCGAAATTCTTTGGTCGATCTCAAAGCAAATCGTGTTTTGGAAGAATTGAGTATAAACAGATTGGTAAACCTAGCTGTCCCGTTTCTGATTTG
AAGCCTTGTCGTACATTTTATTTGGGTATTTTTTATTGGGAATCGAAAATTTGTTCTGAATCTCCTTATGGTTTCGTTGCGTTCCGAATTACCTCTCTGA
TTTAGAAACCCTAATTTCGGACTTGCCTATGCT

Start of BLAST Alignment:< 435 / Exon 1 (Intron 1 in 5’ UTR)
First 15 bp Perfect Match:< 435 / Exon 1 (Intron 1 in 5’ UTR)
BLAST Details:
Query Sequence: 19520_LB1D 
Length: (533 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At5g20920 68418.t02251 eukaryotic translation initiation ...  1547  1.5e-65   1
At5g20090 68418.t02166 expressed protein contains Pfam do...   227  0.024     1
At1g54790 68414.t05728 GDSL-motif lipase/hydrolase family...   185  0.97      1


>At5g20920 68418.t02251 eukaryotic translation initiation factor 2 subunit 2,
           putative / eIF-2-beta, putative similar to SP|P41035 Eukaryotic
           translation initiation factor 2 subunit (eIF-2-beta) {Oryctolagus
           cuniculus}; contains Pfam profile PF01873: Domain found in IF2B/IF5
        Length = 2397

  Plus Strand HSPs:

 Score = 1547 (238.2 bits), Expect = 1.5e-65, P = 1.5e-65
 Identities = 325/337 (96%), Positives = 325/337 (96%), Strand = Plus / Plus

Query:   201 CCTGCGCCGCTTACTCTCTCTCAGATTCCTCCCGAAGCAACCTCGTTTACTCTCTACGCC 260
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:    20 CCTGCGCCGCTTACTCTCTCTCAGATTCCTCCCGAAGCAACCTCGTTTACTCTCTACGCC 79

Query:   261 AGTTTCAGCGCTTAGACATCCCTTTGTGAGTTTTCTTCGATTCCTTTTCGAAATTCTT-T 319
             | |||||||||||||||||||||||||||||||||||||||||||||||||| ||||| |
Sbjct:    80 AATTTCAGCGCTTAGACATCCCTTTGTGAGTTTTCTTCGATTCCTTTTCGAA-TTCTTCT 138

Query:   320 GGTCGATCTCAAAGCAAATCGTGTTTT-GGAAGAATTGAGTATAAACAGATTGGTAAACC 378
             ||||||||||| || |||||||||||| ||||||||||||||||||||||||||||||||
Sbjct:   139 GGTCGATCTCAGAGTAAATCGTGTTTTTGGAAGAATTGAGTATAAACAGATTGGTAAACC 198

Query:   379 TAGCTGTCCCGTTTCTGATTTGAAGCCTTGTCGTACATTTTATTTGGGTATTTTTT--AT 436
             ||||||| ||||||||||||||||||||||||||||||||||||||||||||||||  ||
Sbjct:   199 TAGCTGTTCCGTTTCTGATTTGAAGCCTTGTCGTACATTTTATTTGGGTATTTTTTTTAT 258

Query:   437 TGGGAATCGAAAATTTGTTCTGAATCTCCTTATGGTTTCGTTGCGTTCCGAATTACCTCT 496
             ||||||||||||| |||||||||||||| ||||||||| |||||||||||||||||||||
Sbjct:   259 TGGGAATCGAAAAGTTGTTCTGAATCTCGTTATGGTTTTGTTGCGTTCCGAATTACCTCT 318

Query:   497 CTGATTTAGAAACCCTAATTTCGGACTTGCCTATGCT 533
             |||||||||||||||||||||||||||||||||||||
Sbjct:   319 CTGATTTAGAAACCCTAATTTCGGACTTGCCTATGCT 355


>At5g20090 68418.t02166 expressed protein contains Pfam domain, PF03650:
           Uncharacterized protein family (UPF0041)
        Length = 1653

  Plus Strand HSPs:

 Score = 227 (40.1 bits), Expect = 0.024, P = 0.024
 Identities = 169/286 (59%), Positives = 169/286 (59%), Strand = Plus / Plus