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At2g28000 SYNGENTA 21157

Genomic Sequence (from TAIR)
aagtttaaatttaaatttaatttgactatttgtgactatttatattttgcagttttcattatcattttttttaacaaaatctaaatactattttttatat
acaaaatgacctaatatgtatccatttaccttaaacctcttatattttaaaacagagtgagtacattcagtaagagaatggataaaaacgaatttggaat
acacaaactataaaagtttcttgagtactgtttctattattgggccataaacagtgagacaaacaggcccatagaagtaggtgaaaaaggcttattaaga
tcaacggttctctcgttacgaatgcaaagaatcttctggatcctttttctctcagctactctctccatttatctaataaaccctaacccctcctcccaca
ctattcgtttacactaaccacttccttatccacaccaatccagaacgttcctcaaaaagctccctccttttctctcaaaATGGCGTCTGCAAACGCTCTC
TCTTCCGCCTCCGTCCTCTGTTCTTCCCGTCAGgtacaaagtttgttcctttttaccttccttagctacttactaaagaagaaaatgaatcatctttcat
tactttgttttgattggatttgacccttcagtgttatctattttcaaaaacctttctctatctggtttattgggtttttgaagaaagtgattgaatttga
ccctttagtttgttctgtttcaattcaattgcttttctctatttggtttctgggttggtgagaaaaggttaatttttatgggaattagaagaaactgatt
gagattaatgttaatgaatctgctttattgatgttgtgggtatacagAGTAAGTTGGGAGGAGGAAATCAGCAGCAAGGTCAGAGAGTTAGCTACAACAA
GAGGACAATCAGACGTTTTAGTGTTAGAGCTAATGTAAAGGAAATAGCTTTTGACCAGCACTCTAGAGCTGCTCTACAAGCTGGTATTGATAAGCTTGCT
GATTGTGTTGGTCTCACTCTTGGCCCTAGAGgtatttgttaatattagtagctcctttactctttccatcttcatctgtgttatttatctttcgac
ttatcttggtgttttcatttgttattcttaagGGAGGAATGTTGTGTTGGATGAATTTGGAAGTCCTAAGGTTGTGAATGATGGAGTCACCATTGCTAGA
GCTATTGAGTTACCTAATGCCATGGAAAATGCTGGTGCAGCGCTTATTCGTGAGgttggtgtagctttgttattgtctctgttaatgctatttatacggt
gcggtaatctggtgttgttatgtggtagGTTGCGAGTAAGACTAATGACTCTGCTGGTGATGGGACAACCACTGCGTCTATCCTTGCTCGGGAGATAATC
AAACATGGATTGTTGAGCGTCACTTCTGGTGCGAATCCCGTTTCACTCAAGAGGGGAATTGATAAGACTGTTCAAGGTCTGATCGAAGAGCTTCAGAAGA
AAGCTAGGCCCGTGAAAGGTCGTGATGACATCAGAGgtactaactttgcagtgaccttccttgtgatgcagcaatgtttgatttcttttactgatcaatc
tgttcttttttttttttccagCTGTGGCTTCTATCTCTGCTGGAAATGATGACCTTATAGGGTCAATGATTGCTGATGCCATCGACAAAGTTGGACCTGA
TGGTGTTTTGTCCATTGAATCTTCATCTTCTTTTGAGACCACGGTCGAAGTTGAAGAAGGGATGGAGgtatgagtttttttgtcttgcttctcttcaact
gtaattttattcaccattcattgccaatgactaacttgattatttacagATTGACAGAGGTTACATCTCGCCTCAGTTTGTTACAAACCCTGAGAAACTA
CTAGCTGAGTTTGAGAATGCTAGGGTATTGATCACTGATCAGAAGATCACTGCGATCAAAGACATCATCCCTATTTTGGAGAAGACCACTCAGCTTCGTG
CTCCGTTGTTGATTATTGCAGAGGATGTTACTGGTGAAGCCTTAGCTACCCTTGTCGTGAACAAGCTTCGTGGTGTCCTCAATGTTGTTGCCGTTAAAGC
GCCAGGATTTGGAGAAAGAAGAAAAGCCATGCTTCAAGATATTGCAATCTTGACAGgtatatgcagatttcgcgtgcgtttagtacttttactttctctc
tgttatggtctaaactttttatcattttgtatgtttttcagGAGCTGAGTACCTAGCCATGGACATGAGCTTACTGGTTGAAAACGCAACCATAGATCAA
TTGGGTATTGCCCGAAAAGTCACGATTAGCAAAGATTCGACTACCCTCATTGCAGATGCAGCTTCCAAGGACGAATTACAAGCTCGGATTGCTCAGCTGA
AGAAAGAACTATTCGAGACTGATTCTGTGTATGACTCAGAGAAGCTCGCTGAGAGAATTGCGAAGCTATCTGGAGGTGTTGCTGTCATTAAAGTCGGAGC
AGCAACTGAAACTGAGCTTGAGGACCGTAAGCTTCGTATCGAGGATGCAAAGAACGCAACATTTGCTGCTATCGAGGAAGGCATAGTTCCAGGTGGTGGT
GCTGCTTTGGTGCATCTCTCCACTGTTATTCCCGCCATTAAGGAGACTTTTGAGGATGCTGATGAACGTTTGGGAGCTGACATAGTACAAAAGgtagcat
ttagtttttcggttcagtatttgttctgtaaccaattgtgactagttatcttataagggtttatgttttgagttcagGCATTGCTGTCACCAGCTGCTCT
TATTGCTCAGAACGCTGGAGTTGAAGGAGAAGTTGTTGTGGAAAAGATTATGTTCAGCGATTGGGAGAATGGGTACAACGCAATGACTGATACATATGAG
AATCTGTTCGAAGCTGGAGTGATTGATCCGGCTAAAGTGACAAGATGTGCGCTACAGAACGCTGCTTCCGTAGCAGGAATGGTACTGACCACTCAGGCCA
TTGTTGTTGACAAACCGAAACCCAAGGCTCCTGCTGCTGCTGCTCCTGAGGGTCTCATGGTGTAAtcaaaacacaacatcttatcatcacaatggcatct
ttgtctttttgcacatttgtcatccccatttatctgcaatgtttttcttttttgggggaatatgagtagtttgttaacagtatcgagaaataattatcat
cttttttgtccaatggaaattgcagaaaatggttatgtatgcgaccaaacattttatatgtagcgagagtagtttagcagaacaataagcaaatatgcgt
acaaactgaataatgttccaacttactatactattaaactaggagggaacaatgttttttcatgcctttactagttttctcataaaacttgatctggata
ttgcacttcgatgatctacgaaccattagcagatctccggtgattcgatacttctccttgtcgtttattgcacaggcacgaactttgacaagcacaagac
atga

Border_1 (21157_RBD)

ATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGA
TTACGCCAAGCTCGAAATTAACCCTCACTAAAGGGAACAAAAGCTGGAGCTCGCTTGAGCTTGGATCAGATTGTCGTTTCCCGCCTTCAGTTTAAACTAT
CAGTGTGACCAACACAATCAGCAAGCTTATCAATACCAGCTTGTAGAGCAGCTCTAGAGTGCTGGTCAAAAGCTATTTCCTTTACATTAGCTCTAACACT
AAAACGTCTGATTGTCCTCTTGTTGTAGCTAACTCTCTGACCTTGCTGCTGATTTCCTCCTCCCAACTTACTCTGTATACCCACAACATCAATAAAGCAG
ATTCATTAACATTAATCTCAATCAGTTTCTTCTCATCTCGACCA

Start of BLAST Alignment:NT 539 / Exon 2 of 8
First 15 bp Perfect Match:NT 534 / Exon 2 of 8
BLAST Details:
Query Sequence: 21157_RBD 
Length: (444 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At2g28000 68415.t03155 RuBisCO subunit binding-protein al...  1157  7.1e-47   1



>At2g28000 68415.t03155 RuBisCO subunit binding-protein alpha subunit,
           chloroplast / 60 kDa chaperonin alpha subunit / CPN-60 alpha
           identical to SWISS-PROT:P21238- RuBisCO subunit binding-protein
           alpha subunit, chloroplast precursor (60 kDa chaperonin alpha
           subunit, CPN-60 alpha) [Arabidopsis thaliana]
        Length = 2924

  Minus Strand HSPs:

 Score = 1157 (179.6 bits), Expect = 7.1e-47, P = 7.1e-47
 Identities = 233/235 (99%), Positives = 233/235 (99%), Strand = Minus / Plus

Query:   436 ATGAGAAGAAACTGATTGAGATTAATGTTAATGAATCTGCTTTATTGATGTTGTGGGTAT 377
             || |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   442 ATTAGAAGAAACTGATTGAGATTAATGTTAATGAATCTGCTTTATTGATGTTGTGGGTAT 501

Query:   376 ACAGAGTAAGTTGGGAGGAGGAAATCAGCAGCAAGGTCAGAGAGTTAGCTACAACAAGAG 317
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   502 ACAGAGTAAGTTGGGAGGAGGAAATCAGCAGCAAGGTCAGAGAGTTAGCTACAACAAGAG 561

Query:   316 GACAATCAGACGTTTTAGTGTTAGAGCTAATGTAAAGGAAATAGCTTTTGACCAGCACTC 257
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   562 GACAATCAGACGTTTTAGTGTTAGAGCTAATGTAAAGGAAATAGCTTTTGACCAGCACTC 621

Query:   256 TAGAGCTGCTCTACAAGCTGGTATTGATAAGCTTGCTGATTGTGTTGGTCACACT 202
             |||||||||||||||||||||||||||||||||||||||||||||||||| ||||
Sbjct:   622 TAGAGCTGCTCTACAAGCTGGTATTGATAAGCTTGCTGATTGTGTTGGTCTCACT 676


Border_2 (21157_LBU)

GCCTTTTCAGAAATGGATAAATAGCCTTGCTTCCTATTATATCTTCCCAAATTACCAATACATTACACTAGCATCTGAATTTCATAACCAATCTCGATAC
ACCAAATCGAATTCAATTCGGCGTTAATTCAGTACATTAAAAACGTCCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATGTGTTAATATTAGTAGCTC
CTTTACTCTTTCCATCTTCATCTGTGTTATTTATCTTTCGACTTATCTTGGGTTTTCATTTGTTATTCTTAAGGGAGGAATGTTGTGTTGGATGAATTTG
GAAGTCCTAAGGTTGTGAATGATGGAGTCACCATTGCTAGAGCTATTGAGTTACCTAATGCCATGGAAAATGCTGGTGCAGCGCTTATTCGTGAGGTTGG
TGTAGCTTTGTTATTGTCTCTGTTAATGCTATTTATACGGTGCGGTAATCTGGTGTTGTTATGTGGTAGGTTGCGAGTAAGACTAATGACTCTGCTGGTG
ATGGGACAACCACTGCGTCTATCCTTGCTCGGGAGATAATCAAACATGGATTGTTGAGCGTCACTTCTGGTGCGAATCCGTTTCACTCAAGAGGGGAATT
GATAAGACTGTTCAAGGTCTGATCGAAGAGCTTCAGAAGAAAGCTAGGCCCGTGAAAGGTCGTGATGACATCAGAGGTACTAACTTTGCAGTGACCTTCC
TTG

Start of BLAST Alignment:NT 554 / Intron 2 of 7
First 15 bp Perfect Match:NT 557 / Intron 2 of 7
BLAST Details:
Query Sequence: 21157_LBU 
Length: (703 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At2g28000 68415.t03155 RuBisCO subunit binding-protein al...  2591  8.7e-113  1
At5g18820 68418.t02025 chaperonin, putative similar to SW...   498  8.8e-17   1
At5g56500 68418.t06543 chaperonin, putative similar to SW...   232  2.2e-06   2
At2g22570 68415.t02515 isochorismatase hydrolase family p...   187  0.99      1


>At2g28000 68415.t03155 RuBisCO subunit binding-protein alpha subunit,
           chloroplast / 60 kDa chaperonin alpha subunit / CPN-60 alpha
           identical to SWISS-PROT:P21238- RuBisCO subunit binding-protein
           alpha subunit, chloroplast precursor (60 kDa chaperonin alpha
           subunit, CPN-60 alpha) [Arabidopsis thaliana]
        Length = 2924

  Plus Strand HSPs:

 Score = 2591 (394.8 bits), Expect = 8.7e-113, P = 8.7e-113
 Identities = 523/526 (99%), Positives = 523/526 (99%), Strand = Plus / Plus

Query:   180 ATGTGTTAATATTAGTAGCTCCTTTACTCTTTCCATCTTCATCTGTGTTATTTATCTTTC 239
             || |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   692 ATTTGTTAATATTAGTAGCTCCTTTACTCTTTCCATCTTCATCTGTGTTATTTATCTTTC 751

Query:   240 GACTTATCTTGG-GTTTTCATTTGTTATTCTTAAGGGAGGAATGTTGTGTTGGATGAATT 298
             |||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   752 GACTTATCTTGGTGTTTTCATTTGTTATTCTTAAGGGAGGAATGTTGTGTTGGATGAATT 811

Query:   299 TGGAAGTCCTAAGGTTGTGAATGATGGAGTCACCATTGCTAGAGCTATTGAGTTACCTAA 358
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   812 TGGAAGTCCTAAGGTTGTGAATGATGGAGTCACCATTGCTAGAGCTATTGAGTTACCTAA 871

Query:   359 TGCCATGGAAAATGCTGGTGCAGCGCTTATTCGTGAGGTTGGTGTAGCTTTGTTATTGTC 418
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   872 TGCCATGGAAAATGCTGGTGCAGCGCTTATTCGTGAGGTTGGTGTAGCTTTGTTATTGTC 931

Query:   419 TCTGTTAATGCTATTTATACGGTGCGGTAATCTGGTGTTGTTATGTGGTAGGTTGCGAGT 478
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   932 TCTGTTAATGCTATTTATACGGTGCGGTAATCTGGTGTTGTTATGTGGTAGGTTGCGAGT 991

Query:   479 AAGACTAATGACTCTGCTGGTGATGGGACAACCACTGCGTCTATCCTTGCTCGGGAGATA 538
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   992 AAGACTAATGACTCTGCTGGTGATGGGACAACCACTGCGTCTATCCTTGCTCGGGAGATA 1051

Query:   539 ATCAAACATGGATTGTTGAGCGTCACTTCTGGTGCGAATCC-GTTTCACTCAAGAGGGGA 597
             ||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
Sbjct:  1052 ATCAAACATGGATTGTTGAGCGTCACTTCTGGTGCGAATCCCGTTTCACTCAAGAGGGGA 1111

Query:   598 ATTGATAAGACTGTTCAAGGTCTGATCGAAGAGCTTCAGAAGAAAGCTAGGCCCGTGAAA 657
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1112 ATTGATAAGACTGTTCAAGGTCTGATCGAAGAGCTTCAGAAGAAAGCTAGGCCCGTGAAA 1171

Query:   658 GGTCGTGATGACATCAGAGGTACTAACTTTGCAGTGACCTTCCTTG 703
             ||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1172 GGTCGTGATGACATCAGAGGTACTAACTTTGCAGTGACCTTCCTTG 1217


>At5g18820 68418.t02025 chaperonin, putative similar to SWISS-PROT:P08926-
           RuBisCO subunit binding-protein alpha subunit, chloroplast precursor
           (60 kDa chaperonin alpha subunit, CPN-60 alpha)[Pisum sativum];
           contains Pfam:PF00118 domain, TCP-1/cpn60 chaperonin family
        Length = 2605

  Plus Strand HSPs:

 Score = 498 (80.8 bits), Expect = 8.8e-17, P = 8.8e-17
 Identities = 248/384 (64%), Positives = 248/384 (64%), Strand = Plus / Plus