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At4g23100 SYNGENTA 23012

Genomic Sequence (from TAIR)

gttcatcagaaccaagatttttttgtcgacacaatcaaaccaaatagataatgatactgttttttatagtagtatatatatttcttttcctatagagtat
tttatctgaggtcatgtggttggttagtgacacacttttcattattttacgttctaaattaaatatcgtaaagttaaacctcgtttaaagtatactataa
agaatagaaatatcattaattcattatacacactataaagttaacctttgtttaaactactaataaactggttatttaaaaaactattagggttaaattt
gtgagaaatggaaaagtaagaggcaaaaaacgaaagaatcttttattacaatgattattaattttatcaactttcttttatagacagctcagctccactg
atcggctcatgctccactgatcaactttctttcatttttttatcagtttctcatttctctctcagtcgcatataaatttgaaacgccgattcgtttttgc
tctttaagctttcttcttgatttcgacggtgtctctcaatctccgtcaagcttgacgaatttcaggtgtgatttgcttctcttcttcttcgctttctgga
cttgtggattttggtttttgattgatttgttctgcttttttttttttggttgaatcttcgctttttctcatgatttttttttggctgttgcatgattttg
gttttgttagagtcgaatttttgcagatttttgctttgatttgatttttgttgttgttgttgtttctggtttggtgatacacgaattttgatttgttctt
ccagatgatagttcaattctgtggtagctgctgattctaattatgtgatatatgttattagaattgagaaaaatgcagttatttgtgatttctcatagct
tatgatgaattgttataattgcaggagctatatataccATGGCGCTCTTGTCTCAAGCAGGAGGATCATACACTGTTGTTCCTTCTGGAGTTTGTTCAAA
GGCTGGAACTAAAGCTGTTGTTTCGGGTGGCGTGAGGAATTTGGATGTTTTGAGGATGAAAGAAGCTTTTGGTAGCTCCTACTCTAGGAGTCTATCTACC
AAATCAATGCTTCTCCATTCTGTTAAGAGGAGTAAGAGAGGGCATCAATTGATTGTTGCGGCAAGTCCTCCAACGGAAGAGGCTGTAGTTGCAACTGAGC
CGTTGACGAGAGAGGATCTCATTGCCTATCTTGCCTCTGGATGCAAAACAAAGGACAAATATAGgtacagtttgatgcttatatgttctgttaaaatgct
acttagtgtttgtaattgtgacatatttctttgtttgtgctcttttacggtgttggtattgtttgaattcatccagattgtttgtctattggagaaatat
tctgttttgctaacttaacattgtgagttttcagAATAGGTACAGAACATGAGAAATTTGGTTTTGAGGTCAATACTTTGCGCCCTATGAAGTATGATCA
AATAGCCGAGCTTCTTAATGGTATCGCTGAAAGATTTGAATGGGAAAAAGTAATGGAAGGTGACAAGATCATTGGTCTGAAGCAGgttaacattctcctg
tgtttcatcttttgagccgtttattatttatatacattttcagcctgtagaacatgttagcttcatatacaacacaatgatcggtatattataaggtttc
agtcttttttttttttttgctgtagataactcgttctgtccttttgttgaccagGGAAAGCAAAGCATTTCACTTGAACCTGGGGGTCAGTTCGAGCTTA
GTGGTGCACCTCTTGAGACTTTGCATCAAACTTGTGCTGAAGTCAATTCACATCTTTATCAGgtaatttcaaagataattattgccaatgctctcaccct
aaaacgtgacaaataaccaactatctacggatgtcacagGTAAAAGCAGTTGCTGAGGAAATGGGAATTGGTTTCTTAGGAATTGGCTTCCAGCCCAAAT
GGCGTCGGGAGGATATACCCATCATGCCAAAGgtatgacgctttctctattcttaagattgaagaaagttaacatctgtttgtttctaattcagataata
ttatctgattctacatagccaacgttcggattatttcttggtgtttgtgttaagttttcctatgacgagtcaggcagtaagatttgacatagtgtaatgt
gttctgtgcagGGGAGATACGACATTATGAGAAACTACATGCCGAAAGTTGGTACCCTTGGTCTTGATATGATGCTCCGAACGTGTA►CTGTTCAGgtta
gattaatattctgactttgcgccttttcataatttccttaaactaaactttccagttctcatcaataatcttattttgaaacttatagaaactgcattac
ttgcaatcttgacagGTTAATCTGGATTTTAGCTCAGAAGCTGATATGATCAGGA◄AGTTTCG►TGCTG◄GTCTTGCTTTACAACCTgtgagaagctcga
tctctttgtggcataaccagaaacttttattaacgattttgactaaccattgcctcactattatctaaattcaacagATAGCAACGGCTCTATTTGCGAA
TTCCCCTTTTACAGAAGGAAAGCCAAACGGATTTCTCAGCATGAGAAGgtatctagattcagttgttatgatccccgatatctttttgaattagtttccc
tcccaccttataactctggtatgttttgacagCCACATATGGACAGACACTGACAAGGACCGCACAGGAATGCTACCATTTGTTTTCGATGACTCTTTTG
Ggtgagtatcgatgtttaatctatgaaaaacaattacattacattggttggtttggtatgagaagcttctcaaaaaccaatgactctgtctcgcattctt
gatgagttattggaaaattgatatgtgctattctgtggattttcttacatatgtttgtgttcaagatagaaagatgctttggatctttatccttctcttg
aaacagcatggctaatcttaattttattgctctcttttgttcttatctggtatagGTTTGAGCAGTATGTTGACTACGCACTCGATGTCCCTATGTACTT
TGCCTACAGAAAGAACAAATACATCGACTGTACTGGAATGACATTTCGGgtcaaaatctatccttaatcttttctgttaggttctataactgtttcattg
caaggaattggcaaattgaaaaccatgggatcttcttgaattatatctaatctcataatattcttcatgctgcagCAATTCTTGGCTGGAAAACTTCCCT
GTCTCCCTGGTGAACTGCCTTCATATAATGATTGGGAAAACCATCTGACAACAATATTCCCAGAGgttaatcatctgcctcaagagtctttcaatcagcc
tatgactttcatttccctgtcttatgttgaagtctgacaacgttccataacactagGTTCGGTTGAAGAGATACTTGGAGATGAGAGGTGCTGATGGAGG
TCCCTGGAGGAGGCTGTGTGCCCTGCCAGCTTTCTGGgtaactacatctattagcacatatatatttcgtgcttaatgagaatgtttcaaaaagtattga
ctgttgttttgattttatctacaaatttcagGTGGGTTTATTATATGATGATGATAGTCTCCAAGCTATCCTGGATCTGACAGCTGACTGGACTCCAGCA
GAGAGAGAGATGCTAAGGAACAAAgtaataacaagacgcaccacatttacattttcttattccacagttagaaaacatcttgtaataaactaatacttga
tttccaacagGTCCCAGTTACTGGCTTAAAGACTCCTTTTAGGGATGGTTTGTTAAAGCATGTCGCTGAAGATGTCCTGAAACTCGCAAAGgtgtgtttg
tatgaaccctcttagaaacttcgagccagctttctatcccgtctggtccagcttctaactattggacttattttctctttgtttctcagGATGGTTTAGA
GCGCAGAGGCTACAAGGAAGCCGGTTTCTTGAACGCAGTCGATGAAGTGGTCAGAACAGgtaaatgaagtttcctaatgcataattgaattccacaccga
cattttgtttgataacctggtggtgaaatttggttttgaaatgacagGAGTTACGCCTGCGGAGAAGCTCTTGGAGATGTACAATGGAGAATGGGGACAA
AGCGTAGATCCCGTGTTCGAAGAGCTGCTGTACTAAgaaaatgggacgtgaacaaaaggtgtctataaacctttgggtgtgagtttatgctatctgaaga
attcgagtctccggaataaggatttttttttttgttgtaatcggattttaaaaactgattttgttttagaaattcgaagcattgaaaagcagaagaaaaa
ttgtatgtactaaacgatttcggtgtgggaaatcgtttgggagggtgtgtttggatcttgaataaattacccatttttcttgtcacaaatttgtctacat
ttaacgaaataactcaaaactgatttcaaggcaaaggcgacggatgggataatctaggctgtgtagtggaaagttttgatgtttagtaatttggcaatgt
atgtttttttctaaatgggagagcatcttcgatgtagacatttacgagcttttcctttaataggttttggctagtgaagaacactgctttcataattgaa
tccaccagactccgcgtaaactaaactgattacaacacgatgatcctggccgtcggtaattatcgtaaaacaagaagatagtggcacttaggtgacagga
taatatgtaaaccctaccaaggcgtccctacaaaatgatgagtgatgactgatgagagtgagaaaaaccgagtctccacagtccacacactgccttacct
aatt

Border_1 (23012_LB2U)


GCCTTTTCAGAAATGGATAAATAGCCTTGCTTCCTATTATATCTTCCCAAATTACCAATACATTACACTAGCATCTGAATTTCATAACCAATCTCGATAC
ACCAAWTCSAATTCAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTACACCACAATATAT
CCTGATCATATCAGCTTCTGAGCTAAAATCCAGATTAACCTGTCAAGATTGCAAGTAATGCAGTTTCTATAAGTTTCAAAATAAGATTATTGATGAGAAC
TGGAAAGTTTAGTTTAAGGAAATTATGAAAAGGCGCAAAGTCAGAATATTAATCTAACCTGAACAGTACACGTTCGGAGCATCATATCAAGACCAAGGGT
ACCAACTTTCGGCATGTAGTTTCTCATAATGTCGTATCTCCCCTGCACAGAACTAAC

Start of BLAST Alignment:	NT 1528 / Exon 7 of 16
First 15 bp Perfect Match:	NT 1516 / Exon 7 of 16

BLAST Details:
Query Sequence: 23012_LB2U 
Length: (457 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At4g23100 68417.t03120 glutamate-cysteine ligase / gamma-...  1285  1.7e-52   1
At1g47128 68414.t04777 cysteine proteinase (RD21A) / thio...   179  0.99      1


>At4g23100 68417.t03120 glutamate-cysteine ligase / gamma-glutamylcysteine
           synthetase (GSH1) identical to glutamate-cysteine ligase SP:P46309
           from [Arabidopsis thaliana]; contains Pfam profile: PF04107
           glutamate-cysteine ligase family 2(GCS2)
        Length = 4120

  Minus Strand HSPs:

 Score = 1285 (198.9 bits), Expect = 1.7e-52, P = 1.7e-52
 Identities = 261/266 (98%), Positives = 261/266 (98%), Strand = Minus / Plus

Query:   453 GTTCTGTGCAGGGGAGATACGACATTATGAGAAACTACATGCCGAAAGTTGGTACCCTTG 394
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1793 GTTCTGTGCAGGGGAGATACGACATTATGAGAAACTACATGCCGAAAGTTGGTACCCTTG 1852

Query:   393 GTCTTGATATGATGCTCCGAACGTGTACTGTTCAGGTTAGATTAATATTCTGACTTTGCG 334
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1853 GTCTTGATATGATGCTCCGAACGTGTACTGTTCAGGTTAGATTAATATTCTGACTTTGCG 1912

Query:   333 CCTTTTCATAATTTCCTTAAACTAAACTTTCCAGTTCTCATCAATAATCTTATTTTGAAA 274
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1913 CCTTTTCATAATTTCCTTAAACTAAACTTTCCAGTTCTCATCAATAATCTTATTTTGAAA 1972

Query:   273 CTTATAGAAACTGCATTACTTGCAATCTTGACAGGTTAATCTGGATTTTAGCTCAGAAGC 214
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1973 CTTATAGAAACTGCATTACTTGCAATCTTGACAGGTTAATCTGGATTTTAGCTCAGAAGC 2032

Query:   213 TGATATGATCAGGATATATTGTGGTG 188
             ||||||||||||||  | | ||| ||
Sbjct:  2033 TGATATGATCAGGAAGTTTCGTGCTG 2058


>At1g47128 68414.t04777 cysteine proteinase (RD21A) / thiol protease identical
           to SP|P43297 Cysteine proteinase RD21A precursor (EC 3.4.22.-)
           {Arabidopsis thaliana}, thiol protease RD21A SP:P43297 from
           [Arabidopsis thaliana]
        Length = 2846

  Minus Strand HSPs:

 Score = 179 (32.9 bits), Expect = 4.4, P = 0.99
 Identities = 166/294 (56%), Positives = 167/294 (56%), Strand = Minus / Plus

Border_2 (23012_LB1D)


GCCTTTTCAGAAATGGATAAATAGCCTTGCTTCCTATTATATCTTCCCAAATTACCAATACATTACACTAGCATCTGAATTTCATAACCAATCTCGATAC
ACCAAATCGAATTCAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTGCTGGTCTTGCTTTA
CAACCTGTGAGAAGCTCGATCTCTTTGTGGCATAACCAGAAACTTTTATTAACGATTTTGACTAACCATTGCCTCACTATTATCTAAATTCAACAGATAG
CAACGGCTCTATTTGCGAATTCCCCTTTTACAGAAGGAAAGCCAAACGGATTTCTCAGCATGAGAAGGTATCTAGATTCAGTTGTTATGATCCCCGATAT
CTTTTTGAATTAGTTTCCCTCCCACCTTATAACTCTGGTATGTTTTGACAGCCACATATGGACAGACACTGACAAGGACCGCACAGGAATGCTACCATTT
GTTTTCGATGACTCTTTTGGGTGAGTATCGATGTTTAATCTATGAWAMWYAATTACATTACATTGGTTGGTTTGGTATGAGAAGCTTCTCAAAAACCAAT
GACTCTGTCTCGCATTCTTGATGAGNTATTGGNAAATTGATATGGCTATCTGGGNATTTTCTTACATATGTTGGGGTCAAGAAAAAAAAGAAGCTTTGGA
TNTTTATNCTTTTCTTGNAACAAGAAGGNTAAACCTAAATTTAATGGGCCCTTTTTGGT

Start of BLAST Alignment:	NT 1349 / Exon 6 of 16
First 15 bp Perfect Match:	NT 1523 / Exon 7 of 16

BLAST Details:
Query Sequence: 23012_LB1D 
Length: (759 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At4g23100 68417.t03120 glutamate-cysteine ligase / gamma-...  2600  2.4e-113  1


>At4g23100 68417.t03120 glutamate-cysteine ligase / gamma-glutamylcysteine
           synthetase (GSH1) identical to glutamate-cysteine ligase SP:P46309
           from [Arabidopsis thaliana]; contains Pfam profile: PF04107
           glutamate-cysteine ligase family 2(GCS2)
        Length = 4120

  Plus Strand HSPs:

 Score = 2600 (396.2 bits), Expect = 2.4e-113, P = 2.4e-113
 Identities = 640/763 (83%), Positives = 644/763 (84%), Strand = Plus / Plus

Query:     3 CTTTTCAGAAATGGATAAATAGCCTTGCTTCCTATTATATCTTCCCA-AATTACCAATAC 61
             || |||||   | ||| ||||   || ||  || ||    |||  || |||| ||  || 
Sbjct:  1880 CTGTTCAGGT-TAGATTAATA---TT-CTGACT-TTGCGCCTTTTCATAATTTCCT-TAA 1932

Query:    62 ATTACACTAGCATCTGAATTTCATAACCAATCTCGATACACCAAATCGAATTCAATTCGG 121
             | || |||  |  | |  | |||| |  |||||  ||     ||| |  ||  ||  | |
Sbjct:  1933 ACTAAACTTTC--CAGT-TCTCATCAATAATCTT-ATTTTG-AAA-CTTATAGAAA-CTG 1985

Query:   122 CGTTAATT-CAGTACATTAAAAACG--TCCGCAATGT-GTTATTAAGTTG-TCTAAGCGT 176
             | ||| || || | | | | |      || | | | | | |   ||| || | | | |  
Sbjct:  1986 CATTACTTGCAAT-CTTGACAGGTTAATCTGGATTTTAGCTCAGAAGCTGATATGATCAG 2044

Query:   177 CAA-TTTGTTGCTGGTCTTGCTTTACAACCTGTGAGAAGCTCGATCTCTTTGTGGCATAA 235
              || |||  |||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2045 GAAGTTTCGTGCTGGTCTTGCTTTACAACCTGTGAGAAGCTCGATCTCTTTGTGGCATAA 2104

Query:   236 CCAGAAACTTTTATTAACGATTTTGACTAACCATTGCCTCACTATTATCTAAATTCAACA 295
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2105 CCAGAAACTTTTATTAACGATTTTGACTAACCATTGCCTCACTATTATCTAAATTCAACA 2164

Query:   296 GATAGCAACGGCTCTATTTGCGAATTCCCCTTTTACAGAAGGAAAGCCAAACGGATTTCT 355
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2165 GATAGCAACGGCTCTATTTGCGAATTCCCCTTTTACAGAAGGAAAGCCAAACGGATTTCT 2224

Query:   356 CAGCATGAGAAGGTATCTAGATTCAGTTGTTATGATCCCCGATATCTTTTTGAATTAGTT 415
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2225 CAGCATGAGAAGGTATCTAGATTCAGTTGTTATGATCCCCGATATCTTTTTGAATTAGTT 2284

Query:   416 TCCCTCCCACCTTATAACTCTGGTATGTTTTGACAGCCACATATGGACAGACACTGACAA 475
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2285 TCCCTCCCACCTTATAACTCTGGTATGTTTTGACAGCCACATATGGACAGACACTGACAA 2344

Query:   476 GGACCGCACAGGAATGCTACCATTTGTTTTCGATGACTCTTTTGGGTGAGTATCGATGTT 535
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2345 GGACCGCACAGGAATGCTACCATTTGTTTTCGATGACTCTTTTGGGTGAGTATCGATGTT 2404

Query:   536 TAATCTATGAWAMWYAATTACATTACATTGGTTGGTTTGGTATGAGAAGCTTCTCAAAAA 595
             ||||||||||+|+++|||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2405 TAATCTATGAAAAACAATTACATTACATTGGTTGGTTTGGTATGAGAAGCTTCTCAAAAA 2464

Query:   596 CCAATGACTCTGTCTCGCATTCTTGATGAGNTATTGGNAAATTGATATG-GCTAT-CTGG 653
             |||||||||||||||||||||||||||||| |||||| ||||||||||| ||||| ||| 
Sbjct:  2465 CCAATGACTCTGTCTCGCATTCTTGATGAGTTATTGGAAAATTGATATGTGCTATTCTGT 2524

Query:   654 GNATTTTCTTACATATGTTGGGGT-CAAGNNNNNNNNGAAGCTTTGGATNTTTATNCTTT 712
             | ||||||||||||||||| | || ||||        || ||||||||| ||||| ||| 
Sbjct:  2525 GGATTTTCTTACATATGTTTGTGTTCAAGATAGAAA-GATGCTTTGGATCTTTATCCTTC 2583

Query:   713 TCTTGNAACAAGAAGGNTAAACCTAAATTTAATGGGCCCTTTT 755
             ||||| ||||  | || ||| | ||| |||| ||  | |||||
Sbjct:  2584 TCTTGAAACAGCATGGCTAATCTTAATTTTATTGCTCTCTTTT 2626