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At2g31530 SYNGENTA 24018

Genomic Sequence (from TAIR)
TCTCAGCGCCGCGTTTTTTCTCTCCTCATCCTCTCGATGAATTCCTCTCAAGCTTGCTTCTTCCATttttctcttagaccaatctcgctttcgcatccct
cctatgccttcctctcgaaacgtaagttttccttccccaaattaagtgcttgggaatcgaaattttaagagattgttgttacctagactagttcaggata
caatcgttagagtttcgttgcttcgaattgattaaaccctaatttcaaaagcacagacctgggtttttcactgttcctgcaaagttgataactttttcac
tgatactatctcttagactagttctcttaacccggaaattctctcaaagtatccaactttatgaacagaaacaattgttgaggcATGTGTTTTGACCTTG
ATAATGTTTTTGCAGGAGACCCATTTCTGTGTTCTCAACCCAGAAAATGCCTTACTACGAATTTGAATATGTCAAGAACGAGACAAGGGCATTCTATACA
AATGAACAGAAGACATCTTCTTATGAAAGAAAGGAAGAGTTTTTCGATAAATTATTCCGATAAGTTCAGAGATGATTCTATGAGTAGCGAGGAGATGCAT
ACAGATGCTTTGGATGTGGAGATTATTCCGCCAGATTCACAGGACATTCGTAATTCACAGAACTCTGCTGTCTCCAACACACTACAGGATGATAGGCCAA
AGTCTTTTAGAAATAGGTTTCTTGATTTTGTTCGGATAAGTTCAGTACTCAATACTGCAGCAGAAAGATTCTTTAAGAGCGAGATAAGGAGGAGGTTGTT
TGTAACGGCTGTTTTGCTTGTACTTAGCCGTGTTGGATATTTTATACCTTTACCTGGATTCGACAGAAGATTAATACCTCAAGATTACCTTAGCTTTGTC
TCGGGTTCTGTTGGTGAGTGTTCTCATgtcatttgagtcttatagtcccttgtttggttgacatttctatgtatttgtagttaacattttatgtttt
tgatagaggaactgggtgagTTTGGAGCAGAGATCAAGCTTTCGCTCTTCCAGCTTGGGCTCAGTCCACAAATAATAGCATCCATAATTATGCAGgttaa
gatctctcctcgatttgatgtgtttgcctgagtctgtgcgttctttatatattaagattatgattgatgataaagtgctcatgaaacaccagGTGCTTTG
TCACGTTCTTCCATCCTTGGTGAAACTGCGAAAGGAAGGCTTAGATGGTCACGAGAAGATCAAGAGTTATATGTGTgtttagtttcttacctctcaagcc
gtgagatgaattcttaacatacaattactttgtttgtcttgtgacttttttgcttcattaaacaaagttactaaatcgcctttttggtcttattttatta
gATGGTGGCTATCGTTTTTCTTCGCTATAGTGGAAGCATTAGTGGTGGCTTACACTTCTCTTCAATATTCAGTTTTTGCAGCCACTGCCCAgtaagttaa
ttaggattcttcacgttcggggtatcaaaatggtgcttttaaattctatcatccattttgccacaactgatgctaaagtaataatgcatgcttattcagg
gtcaagCATGTGATGATGACATCATCTCTGCTAGTCTGTGGTGCAATGACAATGACGTGGCTCTGTGATACAATATCAGAATCTGGATTTGgtatttgat
tctctatatctctatctctataaccattggtagcttaaagtgatgctgatatggagtaattggtggagcttgctcataacctgtttctaagccttttaca
gGTCATGGCTCATCTCTGATTATTTGTGTGGGAATATTGACGGGCTACACAGAGACTTTACACAAGATGCTCAATCAAATCTCAGgtacctttggttttt
cttactccacatttctcaaacttatggttagtctttacattagacctgcatgtcttccacatttcctggaggcaactatcattcgcctatcttctcaatt
gatttataaaaaaaggaagcgttcttctcttttgcagGAAGTTTCTCGAACTGGTTGCCTTACTTGTTAGGCTTGCTGGGCATTTTTACCGTAGTAACTA
TGTTCGCTGTTGTTGTTACTGAGGGCTGCAGGAAGATTAAACTACAATATTATGGTTTTAAACTTGCTTCTGCTTCGAGgtgatatatgtagctcagcat
cattattttatcgaaaagattgttgtataattgccacttttaccaatatagctaggagtagattacggttaaagtacatttggatgcaacgtgtggttta
ctcatatttgtgtttatgctatgttttgccatttgggcagAGAAGGTTCTCCAATAACAGAGGTGGAGCCGTACATACCTTTTAACATCAACCCGGCTGG
AATGCAACCTGTTCTCACAACCACATATCTTTTGGCATTCCCCAGCATTCTTGCAAGgtaaattgcttgaagaacctcttactattagggtttaaatcat
tcctgtttcttacttctttgtgttattgttaccatctaaggctgtctaataaggtaatttattgtttttagTATTCTGGGTTCACCTTTCTTGCTAAACA
TGAAGGAGATATTAAACCCTGAAAGCACAGTTGGAGCACCACCTTGGGTCTATTACTCAATATATGCGTTTTTCGTCTTTCTCTTCAACATATTTGACAT
TgtgagtaaatccattacactttttctcagtctgaatttcagtcttccaatgaacattaaacattacatttaccgggtttcttgttttgttatgtagGCC
AACTTGCCAAAGGAAATAGCGGATTATTTAAACAAAATGGGGGCGAGAATACCGAACATAAAGCCTGGGAAAGCCACAATCGAGTACTTAACGAAGATAC
AAGCATCAACTAGGTTTTGGGgtaagttaagctgtttcattttagatatattgcgtatatggtacaagtataatctctaaaaaccatttcatttggtttg
atgcagGTGGCCTTCTGTTGAGCTTTTTGGCAACAGCTTCTACTGTACTTGACCACTATCTACGCAGCATCAATCAAGGCTTTTCTATCGGATTTACATC
TGTTTTGATCATTgtaagtgttctttccacgtatcaatatcattcagtttagcagtattgatgtcgttgtgttaataatctctattgtttgtgtacagGT
TGGTTCCATTATTGAACTCAGAAGATCTTACCATGCCTACAATGTAATGCCGAGTTTAAGCAAAGCGTTAAAGAGATATGGCGTATAAagggagattgga
gaaacagaagaacttatagatatattattgtcgtttaaactttagaagtttctgtagctcttttgattagtagattaggcattagataatttgtattaag
aaccaaaacacatttccatcataatattagaagacttaaaacccaagggccaagtatcacaagcatttgcggaaagattatgcaggaatagtcgaacttc
ataaatgtctgcaaatactccgagaattattgaacttgaaactgatttcttttcttcgtttgtccagaaaatgagagtcaattgatttagttgtggcata
tttggcactaataggagccaaagttacatttgattcataaacgaaagtccacaactagtcgcattaaagaaacaagaaaattaattgtgtcagttttgca
act

Border_1 (24018_LB2D)

ACAAGGGACTATAAGACTCAAATGACATGAGAACACTCACCAACAGAACCCGAGACAAAGCTAAGGTAATCTTGAGGTATTAATCTTCTGTCGAATCCAG
GTAAAGGTATAAAATATCCAACACGGCTAAGTACAAGCAAAACAGCCGTTACAAACAACCTCCTCCTTATCTCGCTCTTAAAGAATCTTTCTGCTGCAGT
ATTGAGTACTGAACTTATCCGAACAAAATCAAGAAACCTATTTCTAAAAGACTTTGGCCTATCATCCTGTAGTGTGTTGGAGACAGCAGAGTTCTGTGAA
TTACGAATGTCCTGTGAATCTGGCGGAATAATCTCCACATCCAAAGCATCTGTATGCATCTCCTCGTTACCTCATAA

Start of BLAST Alignment:NT 569 / Intron 1 of 11
First 15 bp Perfect Match:NT 569 / Intron 1 of 11
BLAST Details:
Query Sequence: 24018_LB2D 
Length: (377 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At2g31530 68415.t03575 secY family protein low similarity...  1856  1.3e-79   1



>At2g31530 68415.t03575 secY family protein low similarity to SP|P31159
           Preprotein translocase secY subunit {Synechococcus sp}; contains
           Pfam profile PF00344: eubacterial secY protein
        Length = 3057

  Minus Strand HSPs:

 Score = 1856 (284.5 bits), Expect = 1.3e-79, P = 1.3e-79
 Identities = 374/376 (99%), Positives = 374/376 (99%), Strand = Minus / Plus

Query:   376 TATGAGGTAACGAGGAGATGCATACAGATGCTTTGGATGTGGAGATTATTCCGCCAGATT 317
             |||||| || ||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   195 TATGAG-TAGCGAGGAGATGCATACAGATGCTTTGGATGTGGAGATTATTCCGCCAGATT 253

Query:   316 CACAGGACATTCGTAATTCACAGAACTCTGCTGTCTCCAACACACTACAGGATGATAGGC 257
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   254 CACAGGACATTCGTAATTCACAGAACTCTGCTGTCTCCAACACACTACAGGATGATAGGC 313

Query:   256 CAAAGTCTTTTAGAAATAGGTTTCTTGATTTTGTTCGGATAAGTTCAGTACTCAATACTG 197
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   314 CAAAGTCTTTTAGAAATAGGTTTCTTGATTTTGTTCGGATAAGTTCAGTACTCAATACTG 373

Query:   196 CAGCAGAAAGATTCTTTAAGAGCGAGATAAGGAGGAGGTTGTTTGTAACGGCTGTTTTGC 137
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   374 CAGCAGAAAGATTCTTTAAGAGCGAGATAAGGAGGAGGTTGTTTGTAACGGCTGTTTTGC 433

Query:   136 TTGTACTTAGCCGTGTTGGATATTTTATACCTTTACCTGGATTCGACAGAAGATTAATAC 77
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   434 TTGTACTTAGCCGTGTTGGATATTTTATACCTTTACCTGGATTCGACAGAAGATTAATAC 493

Query:    76 CTCAAGATTACCTTAGCTTTGTCTCGGGTTCTGTTGGTGAGTGTTCTCATGTCATTTGAG 17
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   494 CTCAAGATTACCTTAGCTTTGTCTCGGGTTCTGTTGGTGAGTGTTCTCATGTCATTTGAG 553

Query:    16 TCTTATAGTCCCTTGT 1
             ||||||||||||||||
Sbjct:   554 TCTTATAGTCCCTTGT 569


Border_2 (24018_LB1U)

GATATCAAATTGACGCCGGCGATAATTTGTTTTTGATAGAGGAACTGGGTGAGTTTGGAGCAGAGATCAAGCTTTCCGCTCTTCCAGCTTGGGCTCAGTC
CACAAATAATAGCATCCATAATTATGCAGGTTAAGATCTCTCCTCGATTTGATGTGTTTGCCTGAGTCTGTGCGTTCTTTATATATTAAGATTATGATTG
ATGATAAAGTGCTCATGAAACACCAGGTGCTTTGTCACGTTCTTCCATCCTTGGTGAAACTGCGAAAGGAAGGCTTAGATGGTCACGAGAAGATCAAGAG
TTATATGTGTGTTTAGTTTCTTACCTCTCAAGCCGTGAGATGAATTCTTAACATACAATTACTTTGTTTGTCTTGTGACTTTTTTGCTTCATTAAACAAA
GTTACTAAATCGCCTTTTTGGTCGTTATTTTATTAGATGGTGGCTATCGTTTTTCTTCGCTATAGTGGAAGCATTAGTGGTGGCTTACACTTCTCTTCAA
TATTCAGTTTTTGCAGCCACTGCCCAGNAAGTTAATTAGGANTCTTCACGTTCGGGGTATCAAAATGGNGCTTTTAAATTCTATCATCCATTTTNGCCAC
AACTGATGCTAAAGTAATAATGCATGCTTATT

Start of BLAST Alignment:NT 601 / Intron 1 of 11
First 15 bp Perfect Match:NT 607 / Intron 1 of 11
BLAST Details:
Query Sequence: 24018_LB1U 
Length: (632 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At2g31530 68415.t03575 secY family protein low similarity...  2962  1.5e-129  1
At1g21630 68414.t02427 calcium-binding EF hand family pro...   191  0.93      1
At5g52890 68418.t06097 AT hook motif-containing protein c...   182  0.999     1
At5g40260 68418.t04547 nodulin MtN3 family protein simila...   179  0.9999    1


>At2g31530 68415.t03575 secY family protein low similarity to SP|P31159
           Preprotein translocase secY subunit {Synechococcus sp}; contains
           Pfam profile PF00344: eubacterial secY protein
        Length = 3057

  Plus Strand HSPs:

 Score = 2962 (450.5 bits), Expect = 1.5e-129, P = 1.5e-129
 Identities = 602/611 (98%), Positives = 602/611 (98%), Strand = Plus / Plus

Query:    22 ATAATTTGTTTTTGATAGAGGAACTGGGTGAGTTTGGAGCAGAGATCAAGCTTTCCGCTC 81
             ||  | ||||||||||||||||||||||||||||||||||||||||||||||||| ||||
Sbjct:   602 ATTTTATGTTTTTGATAGAGGAACTGGGTGAGTTTGGAGCAGAGATCAAGCTTTC-GCTC 660

Query:    82 TTCCAGCTTGGGCTCAGTCCACAAATAATAGCATCCATAATTATGCAGGTTAAGATCTCT 141
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   661 TTCCAGCTTGGGCTCAGTCCACAAATAATAGCATCCATAATTATGCAGGTTAAGATCTCT 720

Query:   142 CCTCGATTTGATGTGTTTGCCTGAGTCTGTGCGTTCTTTATATATTAAGATTATGATTGA 201
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   721 CCTCGATTTGATGTGTTTGCCTGAGTCTGTGCGTTCTTTATATATTAAGATTATGATTGA 780

Query:   202 TGATAAAGTGCTCATGAAACACCAGGTGCTTTGTCACGTTCTTCCATCCTTGGTGAAACT 261
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   781 TGATAAAGTGCTCATGAAACACCAGGTGCTTTGTCACGTTCTTCCATCCTTGGTGAAACT 840

Query:   262 GCGAAAGGAAGGCTTAGATGGTCACGAGAAGATCAAGAGTTATATGTGTGTTTAGTTTCT 321
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   841 GCGAAAGGAAGGCTTAGATGGTCACGAGAAGATCAAGAGTTATATGTGTGTTTAGTTTCT 900

Query:   322 TACCTCTCAAGCCGTGAGATGAATTCTTAACATACAATTACTTTGTTTGTCTTGTGACTT 381
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   901 TACCTCTCAAGCCGTGAGATGAATTCTTAACATACAATTACTTTGTTTGTCTTGTGACTT 960

Query:   382 TTTTGCTTCATTAAACAAAGTTACTAAATCGCCTTTTTGGTCGTTATTTTATTAGATGGT 441
             |||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
Sbjct:   961 TTTTGCTTCATTAAACAAAGTTACTAAATCGCCTTTTTGGTC-TTATTTTATTAGATGGT 1019

Query:   442 GGCTATCGTTTTTCTTCGCTATAGTGGAAGCATTAGTGGTGGCTTACACTTCTCTTCAAT 501
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1020 GGCTATCGTTTTTCTTCGCTATAGTGGAAGCATTAGTGGTGGCTTACACTTCTCTTCAAT 1079

Query:   502 ATTCAGTTTTTGCAGCCACTGCCCAGNAAGTTAATTAGGANTCTTCACGTTCGGGGTATC 561
             |||||||||||||||||||||||||| ||||||||||||| |||||||||||||||||||
Sbjct:  1080 ATTCAGTTTTTGCAGCCACTGCCCAGTAAGTTAATTAGGATTCTTCACGTTCGGGGTATC 1139

Query:   562 AAAATGGNGCTTTTAAATTCTATCATCCATTTTNGCCACAACTGATGCTAAAGTAATAAT 621
             ||||||| ||||||||||||||||||||||||| ||||||||||||||||||||||||||
Sbjct:  1140 AAAATGGTGCTTTTAAATTCTATCATCCATTTT-GCCACAACTGATGCTAAAGTAATAAT 1198

Query:   622 GCATGCTTATT 632
             |||||||||||
Sbjct:  1199 GCATGCTTATT 1209


>At1g21630 68414.t02427 calcium-binding EF hand family protein contains
           INTERPRO:IPR002048 calcium-binding EF-hand domain; ESTs gb|T44428
           and gb|AA395440 come from this gene
        Length = 7000

  Plus Strand HSPs:

 Score = 191 (34.7 bits), Expect = 2.7, P = 0.93
 Identities = 139/226 (61%), Positives = 139/226 (61%), Strand = Plus / Plus