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At2g37920 SYNGENTA 26619

Genomic Sequence (from TAIR)
ttctctttttacaccaaattaaaggagATGTTGTCGAGCAAAAACGTTGTCGTAGTAGAGGCTTGGAACACCACCACCACAACGCAAACGCAAACGCCAC
ATAGACCGTCACTGTTACACCCAACATTCTATTGGGGTTACAACTGCCAAGTGCTCTTCTCAGGCTGGCCTGGTTCTGACCGTGGGATGTATGCACTCGC
ACTTATCTTCGTCTTCTTTCTGGCGTTCTTAGCTGAGTGGCTTGCTCGGTGCTCTGACGCGTCATCCATCAAACAGGGTGCCGATAAGCTTGCTAAAGTT
GCATTTCGCACGGCTATGTATACCGTCAAGTCCGGCTTTAGCTACCTAGTGATTCTGGCTGTTGTTTCCTTCAACGGTGGTGTTTTCCTAGCCGCGATTT
TCGGGCATGCTCTTGGGTTCGCTGTTTTCCGTGGGCGGGCGTTTAGGAATAGGGATATTCAATAActtgtgagtgtaaccaaccaaaccctatgagaatg
gaatgttttttggttattgatttgatatgtgtgtgtgtgcattcgaatataattaagagagttgttttgtaacattttgtgaaaattgagttgactgaaa
aagtcaacctaaaaattgtaaaatcaattttatattaaagaacaataaaaaaaaaatctggaacggataaaataatttgctcggcttcctcagctccaA
TGGCGGCGACGATGATCGGAATTAACCTCTCCTGCTTCAAATCAACATCGTTCTTCTCACCGGACCTCAATTCTCTCCAATCAAAGCTCTCTCTCCTA
TCTCTCAAACCCTCTCCAACACAGCCACGGAAATCAACAGTCATCCGTATGGGCGGTGGTCCGAGAACTTTCCCCGGCGGTGTATCAAAATGGCAATGGA
AAAGGATGCAAGCGAAGAAACAGAAACAGCTTCTCAAAGCAAGGTTATGCAGAGAACGTCAGATCTACGAGATGCGAAAACGCGCCGAGCTAAAAGCGGC
GGTGGCTGAGCTAGAACGACCATGGGAACCGATTCATAAACCACCGAATCTATTCTCAGTTTGTGCTGATGAGCAAGTTAAAGTACTCGCGGATCGGTTT
CAGAAACCTGGTGGATTTGATTTATGGACTGATAGAGATGGTCCTCAATTGTTTGAGAGTGTTGATGATTTGCCTTCTGCTAGGTTTTTTCCTAAAGGTG
TTGTTCATAGTGTTAAACCTTATGGTAGATTATCATCTAGCTCTGTTGTTGATGATGGTGATGAGAGTGAGGTTAAAGATGAAGAAATTGGGAAGAAGTT
ACGTGGTCGTAGGGTGAGGAAGAGAGGTGATGATGGAGGTAAGAGGAGGACTGAAAATCGTGGTAATGGTGGGAGATTGAGAAATGGAGGGTCTTCTTCT
TCTCAGGTGTATGATATGACTTTGCAGAATGATGGGAGATATGAAATTGGATCTTAGgtatgagttcttctttgttgtgtttggatcattgatgtctctg
aacataatgtgtatgtaatctggaatagtttgattgtgattatgcaaagttttcagttttagggaagcaagtctctgtgttcaagtataggttttggttg
atggattattgattggtataagcattgtctataaactggttaacccaccaaagtccttaaaggttggtccttttacctctacatcacaattcttacttcc
ctcttcactagatatacctttcatcttcattttcttcattgtatcggcacaaaaaggcgtcatggatttattagagtaagaaacacgtaaaccatcattg
gtgacatcaaactgcatgttatcttccgatgtcaatacatttccggatgatgaagcagaataaggcgatgatgatatacataaatccgaattcctctgtt
ctg

Border_1 (26619_RescuedLB_U)

AATCRAATTCA:TTCGGCGTTAATTCAGTACATTAAAAACGT:CCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTAAATTATTTTATCCGTT
CCAGATTTTTTTTTTATTGTTCTTTAATATAAAATNGATTTTACAATTTTTAGGTTGACTTTTTCAGTCAACTCAATTTTCACAAAANGTTACAAAACAA
CTNTTTTAATTATATTCGAANGCCCNCNCNCNCATATCAAATCAATANCCAAAAAACATTCCATTNTCATAGGGTTNGGTNGGTTNCNCTCACAAGTTAT
TGAATATCCCTATTCCTAAACGCCCGCCCACGGAAAACAGCGAACCCAANAGCATGCCCGAAAATCGNGGNTAGGAAAACNCCNCCGTTGAAGGAAACAA
CAGCCANAATCACTAGGTAGCTAAAGCCGGACTTGACGGTTTACATAGCCGGGCGAAATGCAACTTTAGCAAGCTTATCGGCCCCCTGTTTGATGGATGA
CGCGTCAAAGCNCCGAGCAAGCCACTCAGTTAAAAACGCCAGAAAGAARACGAAGATAAGGTGCGAGGGCATACATCCCACGGTCAAAACCAGGCCAGCC
TGAAAAGANCACTTGGCAGGTTGTAACCCCAATAAAATGTTGGGGGGTAACANGTGACGGTCTATGKGGCGTTTGCGTTTTGCGTTGGGGGGGGGGGGGG
TTCCAAGGCCTTTACTACNANAACGTTTTTKGTYSNAMAACATCNTCCTTTTAATTTGGGG

Start of BLAST Alignment:NT < 20 / 5’ UTR
First 15 bp Perfect Match:NT < 20 / 5’ UTR
BLAST Details:
Query Sequence: 26619_RescuedLB_U 
Length: (759 letters) 
Database: TIGR AGI BAC Sequences (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

T8P21 44466                                                   2663  4.2e-114  1
T20P8 42106                                                    345  2.3e-07   1
MUF9 67894                                                     205  0.79      1



T8P21 44466
        Length = 128,150

  Minus Strand HSPs:

 Score = 2663 (405.6 bits), Expect = 4.2e-114, P = 4.2e-114
 Identities = 578/652 (88%), Positives = 583/652 (89%), Strand = Minus / Plus

Query:   733 SRACMAAAAACGTTNTNGTAGTAAAGGCCTTGGAANNNNNNNNNNNNNNAACGCAAAACG 674
             ++| +||||||||| | |||||| |||| ||||||              |||||||| ||
Sbjct: 74409 CGAGCAAAAACGTTGTCGTAGTAGAGGC-TTGGAACACCACCACCAC--AACGCAAA-CG 74464

Query:   673 CAAACGCCMCATAGACCGTCACNTGTTACCCCCCAACATTTTATTGGGGTTACAACCTGC 614
             ||||||||+||||||||||||| |||||| ||| |||||| ||||||||||||||| |||
Sbjct: 74465 CAAACGCCACATAGACCGTCAC-TGTTACACCC-AACATTCTATTGGGGTTACAAC-TGC 74521

Query:   613 CAAGTGNTCTTTTCAGGCTGGCCTGGTTTTGACCGTGGGATGTATGCCCTCGCACCTTAT 554
             |||||| |||| |||||||||||||||| |||||||||||||||||| ||||||| ||||
Sbjct: 74522 CAAGTGCTCTTCTCAGGCTGGCCTGGTTCTGACCGTGGGATGTATGCACTCGCAC-TTAT 74580

Query:   553 CTTCGTYTTCTTTCTGGCGTTTTTAACTGAGTGGCTTGCTCGGNGCTTTGACGCGTCATC 494
             ||||||+|||||||||||||| ||| ||||||||||||||||| ||| ||||||||||||
Sbjct: 74581 CTTCGTCTTCTTTCTGGCGTTCTTAGCTGAGTGGCTTGCTCGGTGCTCTGACGCGTCATC 74640

Query:   493 CATCAAACAGGGGGCCGATAAGCTTGCTAAAGTTGCATTTCGCCCGGCTATGTAAACCGT 434
             |||||||||||| |||||||||||||||||||||||||||||| |||||||||| |||||
Sbjct: 74641 CATCAAACAGGGTGCCGATAAGCTTGCTAAAGTTGCATTTCGCACGGCTATGTATACCGT 74700

Query:   433 CAAGTCCGGCTTTAGCTACCTAGTGATTNTGGCTGTTGTTTCCTTCAACGGNGGNGTTTT 374
             |||||||||||||||||||||||||||| |||||||||||||||||||||| || |||||
Sbjct: 74701 CAAGTCCGGCTTTAGCTACCTAGTGATTCTGGCTGTTGTTTCCTTCAACGGTGGTGTTTT 74760

Query:   373 CCTANCCNCGATTTTCGGGCATGCTNTTGGGTTCGCTGTTTTCCGTGGGCGGGCGTTTAG 314
             |||| || ||||||||||||||||| ||||||||||||||||||||||||||||||||||
Sbjct: 74761 CCTAGCCGCGATTTTCGGGCATGCTCTTGGGTTCGCTGTTTTCCGTGGGCGGGCGTTTAG 74820

Query:   313 GAATAGGGATATTCAATAACTTGTGAGNGNAACCNACCNAACCCTATGANAATGGAATGT 254
             ||||||||||||||||||||||||||| | |||| ||| |||||||||| ||||||||||
Sbjct: 74821 GAATAGGGATATTCAATAACTTGTGAGTGTAACCAACCAAACCCTATGAGAATGGAATGT 74880

Query:   253 TTTTTGGNTATTGATTTGATATGNGNGNGNGGGCNTTCGAATATAATTAAAANAGTTGTT 194
             ||||||| ||||||||||||||| | | | | || ||||||||||||||| | |||||||
Sbjct: 74881 TTTTTGGTTATTGATTTGATATGTGTGTGTGTGCATTCGAATATAATTAAGAGAGTTGTT 74940

Query:   193 TTGTAACNTTTTGTGAAAATTGAGTTGACTGAAAAAGTCAACCTAAAAATTGTAAAATCN 134
             ||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||| 
Sbjct: 74941 TTGTAACATTTTGTGAAAATTGAGTTGACTGAAAAAGTCAACCTAAAAATTGTAAAATCA 75000

Query:   133 ATTTTATATTAAAGAACAATNNNNNNNNNNTCTGGAACGGATAAAATAATTT 82
             ||||||||||||||||||||          ||||||||||||||||||||||
Sbjct: 75001 ATTTTATATTAAAGAACAATAAAAAAAAAATCTGGAACGGATAAAATAATTT 75052


T20P8 42106
        Length = 99,345

  Minus Strand HSPs:

 Score = 345 (57.8 bits), Expect = 2.3e-07, P = 2.3e-07
 Identities = 196/333 (58%), Positives = 197/333 (59%), Strand = Minus / Plus


Border_2 (26619_LBD)

AATCGAATTCAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTACACCACAATATATCCTG
CTTCAAATCAACATCGTTCTTCTCACCGGACCTCAATTCTCTCCAATCAAAGCATCTCTCTCCTATCTCTCAAACCCTCTCCAACACAGCCACGGAAATC
AACAGTCATCCGTATGGGCGGTGGCTCCGAGAACTTTCCCCGGCGGTGTATCAAAATGGCAATGGAAAAGGATGCAAGCGAAGAAACAGAAACAGCTTCT
CAAAGCAAGGTTATGCAGAGAACGTCAGATCTACGAGATGCGAAAACGCGCCGAGCTAAAAGCGGCGGTGGCTGAGCTAGAACGACCATGGGAACCGATT
CATAAACCACCGAATCTATTCTCAGTTTGTGCTGATGAGCAAGTTAAAGTACTCGCGGATCGGTTTCAGAAACCTGGTGGATTTGATTTATGGACTGATA
GAGATGGTCCTCAATTGTTTGAGAGTGTTGATGATTTGCCTTCTGCTAGGTTTTTTCCTAAAGGTGTTGTTCATAGTGTTAAACCTTATGGTAGATTATC
ATCTAGCTCTGTTGTTGATGATGGTGATGAGAGTGAGGTTAAAGATGAAGAAATTGGGAAGAAGTTACGTGGTCGTAGGGTGAGGAAGAGAGGTGATG

Start of BLAST Alignment:NT 24 / Exon 1 of 1
First 15 bp Perfect Match:NT 30 / Exon 1 of 1
BLAST Details:
Query Sequence: 26619_LBD 
Length: (698 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At2g37920 68415.t04293 expressed protein                      2988  3.1e-130  1
At2g07750 68415.t00938 DEAD box RNA helicase, putative si...   394  8.2e-11   1
At1g62760 68414.t06492 invertase/pectin methylesterase in...   214  0.16      1
At4g09300 68417.t01467 expressed protein                       210  0.31      1


>At2g37920 68415.t04293 expressed protein
        Length = 963

  Plus Strand HSPs:

 Score = 2988 (454.4 bits), Expect = 3.1e-130, P = 3.1e-130
 Identities = 604/609 (99%), Positives = 604/609 (99%), Strand = Plus / Plus

Query:    90 AATATATCCTGCTTCAAATCAACATCGTTCTTCTCACCGGACCTCAATTCTCTCCAATCA 149
             ||  | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:    72 AACCTCTCCTGCTTCAAATCAACATCGTTCTTCTCACCGGACCTCAATTCTCTCCAATCA 131

Query:   150 AAGCATCTCTCTCCTATCTCTCAAACCCTCTCCAACACAGCCACGGAAATCAACAGTCAT 209
             |||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   132 AAGC-TCTCTCTCCTATCTCTCAAACCCTCTCCAACACAGCCACGGAAATCAACAGTCAT 190

Query:   210 CCGTATGGGCGGTGGCTCCGAGAACTTTCCCCGGCGGTGTATCAAAATGGCAATGGAAAA 269
             ||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   191 CCGTATGGGCGGTGG-TCCGAGAACTTTCCCCGGCGGTGTATCAAAATGGCAATGGAAAA 249

Query:   270 GGATGCAAGCGAAGAAACAGAAACAGCTTCTCAAAGCAAGGTTATGCAGAGAACGTCAGA 329
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   250 GGATGCAAGCGAAGAAACAGAAACAGCTTCTCAAAGCAAGGTTATGCAGAGAACGTCAGA 309

Query:   330 TCTACGAGATGCGAAAACGCGCCGAGCTAAAAGCGGCGGTGGCTGAGCTAGAACGACCAT 389
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   310 TCTACGAGATGCGAAAACGCGCCGAGCTAAAAGCGGCGGTGGCTGAGCTAGAACGACCAT 369

Query:   390 GGGAACCGATTCATAAACCACCGAATCTATTCTCAGTTTGTGCTGATGAGCAAGTTAAAG 449
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   370 GGGAACCGATTCATAAACCACCGAATCTATTCTCAGTTTGTGCTGATGAGCAAGTTAAAG 429

Query:   450 TACTCGCGGATCGGTTTCAGAAACCTGGTGGATTTGATTTATGGACTGATAGAGATGGTC 509
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   430 TACTCGCGGATCGGTTTCAGAAACCTGGTGGATTTGATTTATGGACTGATAGAGATGGTC 489

Query:   510 CTCAATTGTTTGAGAGTGTTGATGATTTGCCTTCTGCTAGGTTTTTTCCTAAAGGTGTTG 569
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   490 CTCAATTGTTTGAGAGTGTTGATGATTTGCCTTCTGCTAGGTTTTTTCCTAAAGGTGTTG 549

Query:   570 TTCATAGTGTTAAACCTTATGGTAGATTATCATCTAGCTCTGTTGTTGATGATGGTGATG 629
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   550 TTCATAGTGTTAAACCTTATGGTAGATTATCATCTAGCTCTGTTGTTGATGATGGTGATG 609

Query:   630 AGAGTGAGGTTAAAGATGAAGAAATTGGGAAGAAGTTACGTGGTCGTAGGGTGAGGAAGA 689
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   610 AGAGTGAGGTTAAAGATGAAGAAATTGGGAAGAAGTTACGTGGTCGTAGGGTGAGGAAGA 669

Query:   690 GAGGTGATG 698
             |||||||||
Sbjct:   670 GAGGTGATG 678


>At2g07750 68415.t00938 DEAD box RNA helicase, putative similar to RNA helicase
           RH25 [Arabidopsis thaliana] GI:3776023; contains Pfam profiles
           PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved
           C-terminal domain
        Length = 4061

  Plus Strand HSPs:

 Score = 394 (65.2 bits), Expect = 8.2e-11, P = 8.2e-11
 Identities = 228/374 (60%), Positives = 228/374 (60%), Strand = Plus / Plus