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At3g57870 SYNGENTA 31703

Genomic Sequence (from TAIR)

aaagaatcttttggtagcttttttgggtatcatcgcgtaggtttcacgccacatttatccattgactttttcattacgtcttttcatttattttatgata
tagtagataataattatacctggttaaaatttaataattgaccggtcaataaccattaaagtggaggaagtaggacgaaaggacatttattcgccgcgaa
atctggaccgtacgatactccgattacccttctaaagctttaacataggggtacatctgtaacttcacattccatcttcccccaatctcctatttgtgtc
ttcttcgagtttatatatagagagatagagattggagagcgagcgagacaaatcagaagagagagatttagatattgtagagtgagattctaaagagaga
gagagagagagatATGGCTAGTGGAATCGCTCGTGGTCGTTTAGCTGAAGAGAGGAAATCGTGGAGGAAGAATCATCCTCATgtaagtgatttcgtgtct
tctatttctccaaattcgtgattttgatttatgggttttgggtttctgttgattgttcatggtctctgattactgattgggtttcttcttgttgatggtg
aaatttagGGTTTTGTGGCAAAGCCGGAGACGGGGCAGGATGGAACTGTGAATCTAATGGTGTGGCATTGCACTATACCTGGTAAAGCTGGGgtaagtat
caatcgtgaaattaggtttcttcagttttcttattcctctgcttgttttcttctggtttgtattagtagcaacaaaaaagagattgaaattagggtttat
tgatttgggctaaagtctttgtttttatcatcttctgattcttagttcttcaaagttttaagcttcaccagtctcttcccttctttttctttgtgattct
gtttgatgattctctcatatttgcttacacatagagccgtggaagattatctaatatgaatgttagtgggaagagtagctgtgtatctatctccttgttt
atcttgtatgatgtttttttattggtgcatatgattttgg►tcttgaatacgacattgaaactgaactctgctgtgtatcttactttgacatggctaata
aga►gtgttactgcaagagcaattgctaatatcaatctttactgttgtttatagctaattttacatgtttctctgctgcagACTGATTGGGAAGGTGGAT
TCTTTCCATTAACGATGCACTTCAGTGAGGATTATCCGAGCAAACCTCCGAAATGTAAATTTCCACAAGGGTTTTTCCACCCTAATGTCTATCCATCTGG
AACTGTCTGTCTCTCTATCCTTAACGAGGATTATgtaagtactctactgtttctttctttgcatttctccatgagcaaaagccagctttatcctcaagct
ctcttttgttatgtgtatatccatagGGATGGAGACCAGCCATCACCGTGAAGCAGATTCTTGTTGGT◄ATTCAGGATTTACTTGACACACCGAAT◄CCC
GCTGACCCTGCACAGACAGATGGTTATCATCTCTTCTGTCAGgtacatttagaaatgaaaactctaaacacaatattaggttttgaaacataggaaggaa
ctaagtcgagtgattgttgatgattattgtgcagGATCCAGTTGAGTACAAGAAAAGGGTGAAGCTGCAGTCCAAGCAGTATCCTGCTCTTGTCTAAaac
ctcaaaaaagagaacgcaccaaaactgatgacaagctctgaatattttctcgtcttgtgtcattatgtttagatgtcattgggtgattgaaaactcctca
ttttggcaccttaagacactggaatctatttattttcgaaaaaaaactcttctttctctatttaactgttaatattcttggtactgattcagttggtgtt
gcttctatataatacataattatgttctctggtcgttcatgagtgttgtccaaattctactaagtgaaatatgaagtcttcattccatagagtaacgaat
cttagttgtgaatctagaaatacatatatttctaacttgaaactacgcttaacacttgaaagtcaaaatcttactcctctaaaatttatactccactgat
ttta

Border_1 (31703_LB2)


GCCTTTTCAGAAATGGATAAATAGCCTTGCTTCCTATTATATCTTCCCAAATTACCAATACATTACACTAGCATCTGAATTTCATAACCAATCTCGATAC
ACCAAATCGAATTCAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTACTGCAAGAGCAATTGCTAATATCAATCTTTACTGTTGTTTA
TAGCTAATTTTACATGTTTCTCTGCTCCTCC

Start of BLAST Alignment:	NT 627 / Intron 2 of 4
First 15 bp Perfect Match:	NT 689 / Intron 2 of 4

BLAST Details:
Query Sequence: 31703_LB2 
Length: (231 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At3g57870 68416.t05971 ubiquitin-conjugating enzyme, puta...   394  3.6e-12   1
At1g16980 68414.t01835 alpha, alpha-trehalose-phosphate s...   168  0.094     1
At1g17800 68414.t01966 plastocyanin-like domain-containin...   157  0.18      1
At4g31860 68417.t04182 protein phosphatase 2C, putative /...   159  0.22      1


>At3g57870 68416.t05971 ubiquitin-conjugating enzyme, putative strong
           similarity to SP|P50550 Ubiquitin-like protein SUMO-1 conjugating
           enzyme (EC 6.3.2.19) (SUMO- 1-protein ligase) (Ubiquitin carrier
           protein) (Ubiquitin-conjugating enzyme UbcE2A) {Xenopus laevis};
           contains Pfam profile PF00179: Ubiquitin-conjugating enzyme
        Length = 1532

  Plus Strand HSPs:

 Score = 394 (65.2 bits), Expect = 3.6e-12, P = 3.6e-12
 Identities = 110/140 (78%), Positives = 110/140 (78%), Strand = Plus / Plus

Query:    92 TCTCGATACACCAAATCGAAT-TCAATTCGGC-GTTAATTCAGTACATTAAAAACGTCCG 149
             ||| || | || | || |||  | || || || ||  || |  ||| || | |  |    
Sbjct:   696 TCTTGA-ATACGACATTGAAACTGAACTCTGCTGTGTAT-CT-TACTTTGACATGGCTAA 752

Query:   150 CAAT-GTGTTACTGCAAGAGCAATTGCTAATATCAATCTTTACTGTTGTTTATAGCTAAT 208
              ||  |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   753 TAAGAGTGTTACTGCAAGAGCAATTGCTAATATCAATCTTTACTGTTGTTTATAGCTAAT 812

Query:   209 TTTACATGTTTCTCTGCTCC 228
             |||||||||||||||||| |
Sbjct:   813 TTTACATGTTTCTCTGCTGC 832


>At1g16980 68414.t01835 alpha, alpha-trehalose-phosphate synthase, UDP-forming,
           putative / trehalose-6-phosphate synthase, putative /
           UDP-glucose-glucosephosphate glucosyltransferase, putative similar
           to trehalose-6-phosphate synthase SL-TPS/P [Selaginella
           lepidophylla] GI:4100325; contains Pfam profiles PF00982:
           Glycosyltransferase family 20, PF02358: Trehalose-phosphatase
        Length = 4178

  Minus Strand HSPs:

 Score = 168 (31.3 bits), Expect = 0.098, P = 0.094
 Identities = 124/207 (59%), Positives = 124/207 (59%), Strand = Minus / Plus

Border_2 (31703_LB1)


GCCTTTTCAGAAATGGATAAATAGCCTTGCTTCCTATTATATCTTCCCAAATTACCAATACATTACACTAGCATCTGAATTTCATAACCAATCTCGATAC
ACCAAATCGAATTCAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTACACCAACAAGAAT
CTGCTTCACGGTGATGGCTGGTCTCCATCCCTATGGATATACACATAACAAAAGAGAGCTTGAGGATAAAGCTGGCTTTTGCTCATGGAGAAATGCAAAG
AAAGAAACAGTAGAGTACTTACATAATCCTCGTTAAGGATAGAGAGACAGACAGTTCCAGATGGATAGACATTAGGGTGGAAAAACCCTTGTGGAAATTT
ACATTTCGGAGGTTTGCTCGGATAATCCTCACTGAAGTGCATCGTTAATGGAAAGAATCCACCTTCCCAATCAGTCTGCAGCAGAGAAACATGTAAAATT
AGCTATAAACAACAGTAAAGATTGATATTAGCAATTGCTCTTGCAGTAACACTCTTATTAGCCATGTCAAAGTAAGATACACAGCAGAGTTCAGTTTCAA
TGTCGTATTCAAGACCAAAATCATATGCACCAATAAAAAACCTTCTTACAAGATAAACAGGAGATAGATACACAGCTACTCTTCCCACTAACATTCATAT
TAGATAATCTTCCACGGCTCTAT

Start of BLAST Alignment:	NT 1080 / Exon 4 of 5
First 15 bp Perfect Match:	NT 1053 / Exon 4 of 5

BLAST Details:
Query Sequence: 31703_LB1 
Length: (723 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At3g57870 68416.t05971 ubiquitin-conjugating enzyme, puta...  2646  5.5e-115  1
At2g46030 68415.t05235 ubiquitin-conjugating enzyme 6 (UB...   195  0.87      1
At5g62540 68418.t07247 ubiquitin-conjugating enzyme 3 (UB...   188  0.99      1
At1g70660 68414.t07454 ubiquitin-conjugating enzyme famil...   186  0.994     1
At1g75310 68414.t08004 DNAJ heat shock N-terminal domain-...   183  0.9998    1


>At3g57870 68416.t05971 ubiquitin-conjugating enzyme, putative strong
           similarity to SP|P50550 Ubiquitin-like protein SUMO-1 conjugating
           enzyme (EC 6.3.2.19) (SUMO- 1-protein ligase) (Ubiquitin carrier
           protein) (Ubiquitin-conjugating enzyme UbcE2A) {Xenopus laevis};
           contains Pfam profile PF00179: Ubiquitin-conjugating enzyme
        Length = 1532

  Minus Strand HSPs:

 Score = 2646 (403.1 bits), Expect = 5.5e-115, P = 5.5e-115
 Identities = 550/567 (97%), Positives = 550/567 (97%), Strand = Minus / Plus

Query:   723 ATAGAGCCGTGGAAGATTATCTAATATGAATGTTAGTGGGAAGAGTAGCTGTGTATCTAT 664
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   586 ATAGAGCCGTGGAAGATTATCTAATATGAATGTTAGTGGGAAGAGTAGCTGTGTATCTAT 645

Query:   663 CTCCT-GTTTATCTTGTAAGAAGGTTTTTTATTGGTGCATATGATTTTGGTCTTGAATAC 605
             ||||| |||||||||||| || | ||||||||||||||||||||||||||||||||||||
Sbjct:   646 CTCCTTGTTTATCTTGTATGATGTTTTTTTATTGGTGCATATGATTTTGGTCTTGAATAC 705

Query:   604 GACATTGAAACTGAACTCTGCTGTGTATCTTACTTTGACATGGCTAATAAGAGTGTTACT 545
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   706 GACATTGAAACTGAACTCTGCTGTGTATCTTACTTTGACATGGCTAATAAGAGTGTTACT 765

Query:   544 GCAAGAGCAATTGCTAATATCAATCTTTACTGTTGTTTATAGCTAATTTTACATGTTTCT 485
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   766 GCAAGAGCAATTGCTAATATCAATCTTTACTGTTGTTTATAGCTAATTTTACATGTTTCT 825

Query:   484 CTGCTGCAGACTGATTGGGAAGGTGGATTCTTTCCATTAACGATGCACTTCAGTGAGGAT 425
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   826 CTGCTGCAGACTGATTGGGAAGGTGGATTCTTTCCATTAACGATGCACTTCAGTGAGGAT 885

Query:   424 TATCCGAGCAAACCTCCGAAATGTAAATTTCCACAAGGGTTTTTCCACCCTAATGTCTAT 365
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   886 TATCCGAGCAAACCTCCGAAATGTAAATTTCCACAAGGGTTTTTCCACCCTAATGTCTAT 945

Query:   364 CCATCTGGAACTGTCTGTCTCTCTATCCTTAACGAGGATTATGTAAGTACTCTACTGTTT 305
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   946 CCATCTGGAACTGTCTGTCTCTCTATCCTTAACGAGGATTATGTAAGTACTCTACTGTTT 1005

Query:   304 CTTTCTTTGCATTTCTCCATGAGCAAAAGCCAGCTTTATCCTCAAGCTCTCTTTTGTTAT 245
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1006 CTTTCTTTGCATTTCTCCATGAGCAAAAGCCAGCTTTATCCTCAAGCTCTCTTTTGTTAT 1065

Query:   244 GTGTATATCCATAGGGATGGAGACCAGCCATCACCGTGAAGCAGATTCTTGTTGGTGTAA 185
             |||||||||||||||||||||||||||||||||||||||||||||||||||||||| |  
Sbjct:  1066 GTGTATATCCATAGGGATGGAGACCAGCCATCACCGTGAAGCAGATTCTTGTTGGTATTC 1125

Query:   184 ACAAATTGACGCTTAGACA-ACTTAAT 159
             |  | || ||  || |||| ||  |||
Sbjct:  1126 AGGATTT-AC--TT-GACACACCGAAT 1148


>At2g46030 68415.t05235 ubiquitin-conjugating enzyme 6 (UBC6) E2; identical to
           gi|431267, SP:P42750, PIR:S52661; contains a ubiquitin-conjugating
           enzymes active site (PDOC00163)
        Length = 2109

  Minus Strand HSPs:

 Score = 195 (35.3 bits), Expect = 2.1, P = 0.87
 Identities = 173/291 (59%), Positives = 173/291 (59%), Strand = Minus / Plus