At1g79560 SYNGENTA 3218
Genomic Sequence (from TAIR)
tggtataaagagttggtgaatggatctttcgagaagcgagagaacaatatcccttccttatataaaagcagcttggacaacaccccaagaaatgtcccta taattaccctttttgccactgtgtctctccttggattccacggtttaaatatattatttttcggatagtgggctgatggaccttaatgggctagcccaat tatataactaataggcttcgagcctctttgagtttaagcgtcatctttctccttcgaagtattaggacactctcaacgacggatcagtgtagataaacct ccttaccggagagaagcgtaagtcttgccggagagtcgagtgagctgaatggttggtactcaccattttccttcagtaattttctcagagagagctcttt aatcgggaaaaatcttcaaatgcgtctggttttgttctctttttaatttttttcttgtgatttcattcATGGAGATTGCAATTTCGTATAAACCGAATCC GCTTATCTCTTCATCGACTCAGTTGTTGAAAAGATCAAAAAGTTTTGGTCTTGTTCGGTTTCCCGCCAAGTATGGACTGGGAGCTACCCGGAAAAAACAG CTCTTTCGAGTCTATGCGTCTGAAAGTAGTAGTGGGTCTTCATCTAATAGCGATGGAGGCTTTTCGTGGGTGAGATTGGCTCAGTCTATTCGCCTTGGTG CCGAGCGGATTGGGGAGAAGATTGGAGAATCTGTGAAGACAGAAATTGGGTTTGACTCGGAAGAAGCAAGTGGGAGAGTGAATGAGTATGTGGCTCGAGT TAAGGATAGTGTGCACAAGGGCCATCACGAGCTGACTCGCTTTAAGAATGAGACAGTGCCTTCGTTTATTGATTGGAACAAGTGGGAGCATTGGAAGgta agctggttcagctttttaacgtaattatcaaatttctgatt◄ctcaagtattgttagacttctgcttgtgttc◄taa►aac►attgagggacaaacgtat ggtatttatttattttgttgttgatttaacggaagcataaattttggaggagactacaactacgcttattctagcgacatatactagtgtattaggttgt ttcttaatgagggaagatgtattgtatcagGACATCAGGAATTGGGACGGTAAACGAGTTGCTGCCTTGTTCATATATGCTTTTGCGCTGTTACTTTCTT GTCAAAGAGTTTATGTTGCCATCCAAGCTCCTCGGGTAGAACGAGAGAGAAGAGAGTTAACAGAGTCTTTTATGGAGGCTTTGATCCCCGAGCCATCTCC TGGAAATATAGAAAAgtaggtgcttcctaatcctcagtttccgattgtaattttctctactgggacatggtaaaatatggttcatattcaaactatggta tcatcctttaacaagtaaatactcagctaatgaggcatctaaagcgtcaagagtgggaagactaaaagaacttctgtttatttgattttgtagaatgtcc tgacttattctagtgtttcagGTTCAAGAGAAATATGTGGAGGAAAGCAACACCTAAAGGCTTGAAATTAAAAAGGTTCATTGAAGCGCCTGATGGAACA CTTGTCCACGATAGTTCTTATGTTGGAGAAAATGCGTGGGATGACGATCTAGAGACCACAGAGGGATCTCTCAAGAAAATAATTGGTAGAAATGCTAGGA TTCAGACAGAGGCAAAGAAGAAACTAAGCCAAGATCTGGGTGTCTCTGgtcagtatacattcgttaggtgatacttgtttagtagtggcatgctgaagat ataacaagaagtgatttattgtgcgaaacctttgtttttgacgacctaatcttatctcactaatcaagtttctttcagagcagcacttctagatccctca tctactatcattggtaacctgtgcttttgaccatctaatcctctattgcattcttctatgatagGTGAAATTGGAGACAGTGTAGGTAATTGGCGGGAAA GGCTTGCAACCTGGAAGGAAATGTTAGAACGAGAAAAATTATCAGAACAGTTAAACTCCTCAGCGGCGAAGTATGTGGTTGAATTTGATATGAAAGAGGT AGAGAAAAGTCTTCGCGAAGATGTTATTGGAAGGACATCTGAAACTGAGGGAACTAGAGCTCTCTGGATATCAAAGAGATGGTGGCGATATCGCCCTAAG CTTCCCTATACCTACTTCCTTCAGAAGCTTGATTCTTCTGAGgtacagctctaacctatactcttgtccggctttgggattgtgagtggcaagtgtcatt atatccagttgaattttttaaatgataacaagtgatggtatattcattatgagttaaaatatgatgaatatattccttttccaaagcaattaatttgaag ctggacgtgataggatgatagatcttttttttagcttatgttggcacgataatctgtgtatgtgttcataacagGTTGCAGCTGTTGTTTTCACGGAAGA CCTAAAGAGATTGTATGTGACCATGAAAGAAGGTTTCCCTCTAGAATATATTgtaagtaaaaaaattatagttgcaagtatttggaaatgaatgatgtac attcaccatgtcatatcctttttatttctagGTTGACATTCCCTTAGATCCTTACTTGTTTGAGACTATTTGCAACGCCGGAGTTGAGGTGGATCTTCTT CAGAAGAGGCAGATCCACTACTTCATGAAAGTTTTCATTGCACTTCTGCCGGGGATACTAATTTTATGGTTTATAAGAGAATCTGCCATGCTTCTCCTTA TCACATCCAAGCGCTTTCTCTACAAGAAGTATAATCAGCTGTTTGATATGGCTTATGCAGAAAATTTTATATTGgtaatcatcgttactgtataccatta acctcgttacttctttgtttagttgccttcttcccacatattcacacttctattgatgagttttaattatcatacagCCGGTTGGAGATGTCAGTGAGAC AAAATCAATGTATAAGGAAGTGGTACTAGGCGGTGATGTCTGGGATCTTCTTGATGAGCTAATGATCTACATGGGGAACCCAATGCAATACTATGAAAAA GATGTGGCTTTTGTCAGGgtaaattacatcttgacccaaaaactaacatgtggatattggtttattccatttaactgtctaagctaagctcaaactctcc tctgttttcatttttgagtcagGGTGTGCTTCTTTCTGGACCTCCTGGAACTGGAAAAACACTTTTTGCGCGAACACTTGCAAAGGAAAGTGGGCTACCA TTTGTGTTTGCATCTGGTGCGGAGTTCACAGACAGTGAAAAAAGTGGTGCTGCAAAGATCAATGAGATGTTTTCAATCGCGAGACGAAATgtaaggttct tagttatgtgttttaaagctcatacggtatagttgtcttctactcacactattattgtggatctaattttatatactatctccagGCCCCTGCTTTTGTG TTTGTGGATGAAATAGATGCTATTGCTGGTAGACATGCTAGAAAAGATCCACGGAGAAGAGCAACTTTTGAAGCTTTAATTGCGCAACTCGATGGGGAgt aagagaatctgcacttgtttataaatcttagtgatatgtacatgttttcatcgggtttgtatcaaggctttcatacttaatattgtaccacctgcagGAA AGAGAAGACAGGTATCGATAGGTTTTCTTTGAGACAAGCTGTGATATTTATCTGTGCTACCAATAGACCTGATGAACTCGACCTTGAGTTTGTCCGTTCT GGCCGTATTGACCGTCGGCTGTATATTGGGTTGCCTGATGCAAAGCAAAGGGTGCAAATATTTGGAGTTCACAGTGCTGGGAAGAACCTTGCAGAAGATA TAGACTTTGGGAAGgcaagttgttttcctgctatttttcaatcccaataacatgtttgatataggtgtttgctcattcataacaactatatcctttttca catccagCTTGTTTTTCGAACGGTTGGCTTTTCCGGGGCAGATATCCGGAATCTTGTTAACGAAGCGGCTATAATGTCGgtatgtcatattcttatgtgg atctcaatttcccaatttagttataccatttctagaggagcgttgttgatcgtaatccaattttacagGTAAGGAAGGGACGTTCTTACATATATCAACA AGACATTGTTGATGTGTTAGATAAACAATTGCTCGAGGGTATGGGTGTACTTCTTACAGAGGAAGAGCAACAGAAATGTGAACAAAGTgttcgtactgac atcctttgtactatttatctcaaaacatactatgctccacagctaaaaaagccctaaccgttaaagcttatgtaatattagGTATCTTACGAAAAGAAGA GACTTCTGGCTGTTCATGAGGCTGGTCATATAGTGTTGGCTCATTTGTTTCCTCGATTTGACTGGCATGCATTTTCGCAGCTCCTTCCTGGTGGCAAGgt ctgtttcaaatatatatttttcagttgtaatgatagtggtgtttgaacactcaacagtcaacacagtgtaagaatggattaaataaacttttctcttgaa tcttacagGAAACTGCTGTATCGGTTTTCTATCCACGTGAAGACATGGTAGACCAAGGGTATACCACGTTTGGTTATATGAAAATGCAAATGGTGGTAGC TCATGGTGGACGCTGTGCTGAACGTGTGGTTTTTGGCGATAATGTCACTGATGGAGGAAAAGATGACCTGGAGAAGATAACAAAAgtaagtactctccct ttctcgtttaactaattactgtgccttgcttttcagcttctgattaaaccttcaactcggtagctaaaataacatctatgtaactgcatatctgcaaagt gcttgtgttttgagtaatattaatgtttagtcttccattctaaatttgacctgacattacattttcagATTGCCAGGGAGATGGTGATTAGCCCTCAAAG TGCGAGATTAGGTCTCACTCAACTTGTAAAAAAGATTGGAATGGTGGATTTACCAGATAACCCAGACGGCGAGCTGATAAAATACCGGgtatatccatta atcagagactgatactatcccatttttgctgatcttacatattctcagtagttcctcacgttcttttcaacttggtgatgttatatcagTGGGACCATCC TCATGTAATGCCAGCAGAGATGTCGGTAGAAGTATCCGAGCTATTCACCCGTGAGTTGACAAGGgtaatttatcttcgactcagcacacctaatctgatt taaactctcccatgtggagcttgaacatatagatggtatttgaatgttggtgacagTATATCGAGGAGACTGAAGAACTTGCGATGAATGCTCTGAGAGC AAACAGGCACATTCTGGACTTAATCACCAGAGAGTTATTAGAAAAATCAAGGATTACTGGATTGgtacactttctgaaacttcaatgacaattcattatg accttttatgtgcttagatactctcagtctgaagaaatattgctttcattttgtagGAAGTGGAAGAGAAAATGAAGGATCTTTCTCCGTTAATGTTTGA GGATTTTGTGAAGCCATTCCAGATAAACCCTGATGATgtagtctatatattatcttcttgttgatcctcaatcctcagctctagttcccattttaagtgt gtgacactagttgttctttcttttcagGAGGAGCTTTTACCTCACAAGGATCGAGTGAGCTATCAGCCAGTTGATTTACGTGCTGCGCCACTCCACAGAA GCTAGaagctttactcttatcctgtcacaccacaggattgttcccaacttcttcaatagtgaaaaccaaaaaggtttgatcaatctaaaaaaatcatctt aaaagagcttttgccccccatcattggtttaggtatagaatatccgcagttatgttcctggcccagccggattttgtactgtgtctgtctgtatttgatg atatatatctgcagttgaacattttcagagaaaccatatgatatgctcacataagaaaacctttcctgttatttgcctggtcaagaattcttcatctttg caaacacagtgcatagctataaaagggatgcatgtgtattcaacagatacatgtgacaaaacaatttcaaccaactggaataaacttttcaacttttttt tgtttgtttttgttaaagaacaaaaaaaggaaaggagaaggatcaagaaatatctctctccaaaccccaaatcgaaaaagtataaaagaattggactctt tgtaatcgacttatgttctcttttcactgcacataagaaaaagaaaggtttttcatttggaagccctgtgagctgatggcttggattgagttgaagttgc tgct
Border_1 (3218_LB1U)
GAAAATTGGTAATTACTCTTTCTTTTTCTCCATATTGACCATCATACTCATTGCTGATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATT
TACAATTGATCAATCAGAAATTTGATAATTACGTTAAAAAGCTGAACCAGCTTACCTTCCAATGCTCCCACTTGTTCCAATCAATAAACGAAGGCACTGT
CTCATTCTTAAAGCGAGTCAGCTCGTGATGGCCCTTGTGCACACTATCCTTAACTCGAGCCACATACTCATTCACTCTCCCACTTGCTTCTTCCGAGTCA
AACCCAATTTCTGTCTTCACAGATTCTCCAATCTTCTCCCCAATCCGCTCGGCACCAAGGCGAATAGACTGAGCCAATCTCACCCACGAAAAGCCTCCAT
CGCTATTAGATGAAGACCCACTACTACTTTCAGACGCATAGACTCGAAAGAGCTGTTTTTTCCGGGTAGCTCCCAGTCCATACTTGGCGGGAAACCGAAC
AAGACCAAAACTTTTTGATCTTTTCAACAACTGAGTCGATGAAGAGATAAGCGGATTCGGTTTATACGAAATTGCAATCTCCATGAATGAAATCACAAGA
AAAAAATTAAAAAGAGAACAAAACCAGACGCATTTGAAGATTTTTCCCGATTAAAGAGCTCTCTCTGAGAAAATTACTGAAGGAAAATGGTGAGTACCAA
CCATTCAGCTCACTCGACTCTCCGGCAAGACTTACGCTTCTCTCCGGTAAGGAGGTTTATCTACACTGATCCGTCGTTGAGAGTGTCCTAATACTTCGAA
GGAGAAAGATGACGCTTAAACTCAAAGAGGCTCGAAGCCTATTAGTTATATAATTGGGCTAGCCCATTAAGGTCCATCAGCCCACTATCCGAAAAATAAT
ATATTTAAACCGTGGAATCCAAGGAGAGACACAGTGGCAAAAAGGGTAATTATAGGGACATTTCTTGGGGTGTTGTCCAAGCTGCTTTTATATAAGGAAG
GGATATTGTTCTCTCGCTTCTCGAAAGATCCATTCACCAACTCTTTATACCAGCCTCTGTAACGTTCGATCATCTTGTATTTCATTTCAACCACTCATGT
AAGTATATTCTTTCTTCAGATTCCGATTGTTTTCTTCGATTAAGTTTTGAGCTAGATTCTCTAGATCTGTTTCACCGATTTGGTTGATTTCTCCGTCAAT
CTGAACTCGCGGTGTGCATCTTGTTGTTTGCAGTGTTTTTAGTTTGTTTTACGAAGAATCAACTATGGCGACGAAGAGAAGCGTTGGAACTTTGAAGGAA
GCGGATCTGAAGGGAAAGAGTGTGTTCGTGAGGGTTGATCTCAACGTTCCTTTGGATGATAACTCTAACATCACCGATGACACCAGGATTCGTGCCGCTG
TTCCCACCATCAAGTACTTGATGGGTAATGGATCTAGGGTTGTTCTCTGCAGTCACTTGGTATGATATCTTATCTCAGTTCTCACTTCACTACTTGCTTA
TCTGTTGTAATTTTGGTGTGGGAATTTTGATCTGATCTGTTTATCCGGAGAGCCATGCGTATAAGAAGAGTCTCTTTGCATGTTAGGATGTAGATATAGT
ATTCTTGTTTTTCAATATGTTCATTGACTCTATCGGTTGATTTTCTGGTGTTGTTTGGGTAATTGTTTTAGTAAGTTTACTTGGATATGTTTATACTCAA
GTTCAATTGGATGATGCTGTTGAATTAATGGATGTATCTGATCTCAATTTATGTTTCAGGGCCGCCCAAAAGGTTTACTCCTAAG
Start of BLAST Alignment: | NT 504 / Intron 1 of 18 |
First 15 bp Perfect Match: | NT 473 / Intron 1 of 18 |
BLAST Details: Query Sequence: 3218_LB1U Length: (1785 letters) Database: Genes from AGI, Total Genome (DNA); Smallest Sum High Probability Sequences producing High-scoring Segment Pairs: Score P(N) N At1g79550 68414.t08477 phosphoglycerate kinase, putative ... 3810 8.9e-168 1 At1g79560 68414.t08478 FtsH protease, putative contains s... 3187 5.1e-139 1 At1g56190 68414.t05913 phosphoglycerate kinase, putative ... 521 6.3e-15 1 At3g10820 68416.t01164 transcription elongation factor-re... 207 0.88 1 >At1g79560 68414.t08478 FtsH protease, putative contains similarity to chloroplast FtsH protease GI:5804782 from [Nicotiana tabacum] Length = 6213 Minus Strand HSPs: Score = 3187 (484.2 bits), Expect = 5.1e-139, P = 5.1e-139 Identities = 657/687 (95%), Positives = 657/687 (95%), Strand = Minus / Plus Query: 766 GTGTAGATAAACCTCCTTACCGGAGAGAAGCGTAAGTCTTGCCGGAGAGTCGAGTGAGCT 707 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 1 GTGTAGATAAACCTCCTTACCGGAGAGAAGCGTAAGTCTTGCCGGAGAGTCGAGTGAGCT 60 Query: 706 GAATGGTTGGTACTCACCATTTTCCTTCAGTAATTTTCTCAGAGAGAGCTCTTTAATCGG 647 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 61 GAATGGTTGGTACTCACCATTTTCCTTCAGTAATTTTCTCAGAGAGAGCTCTTTAATCGG 120 Query: 646 GAAAAATCTTCAAATGCGTCTGGTTTTGTTCTCNNNNNNNNNNNNNNCTTGTGATTTCAT 587 ||||||||||||||||||||||||||||||||| ||||||||||||| Sbjct: 121 GAAAAATCTTCAAATGCGTCTGGTTTTGTTCTCTTTTTAATTTTTTTCTTGTGATTTCAT 180 Query: 586 TCATGGAGATTGCAATTTCGTATAAACCGAATCCGCTTATCTCTTCATCGACTCAGTTGT 527 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 181 TCATGGAGATTGCAATTTCGTATAAACCGAATCCGCTTATCTCTTCATCGACTCAGTTGT 240 Query: 526 TGAAAAGATCAAAAAGTTTTGGTCTTGTTCGGTTTCCCGCCAAGTATGGACTGGGAGCTA 467 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 241 TGAAAAGATCAAAAAGTTTTGGTCTTGTTCGGTTTCCCGCCAAGTATGGACTGGGAGCTA 300 Query: 466 CCCGGAAAAAACAGCTCTTTCGAGTCTATGCGTCTGAAAGTAGTAGTGGGTCTTCATCTA 407 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 301 CCCGGAAAAAACAGCTCTTTCGAGTCTATGCGTCTGAAAGTAGTAGTGGGTCTTCATCTA 360 Query: 406 ATAGCGATGGAGGCTTTTCGTGGGTGAGATTGGCTCAGTCTATTCGCCTTGGTGCCGAGC 347 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 361 ATAGCGATGGAGGCTTTTCGTGGGTGAGATTGGCTCAGTCTATTCGCCTTGGTGCCGAGC 420 Query: 346 GGATTGGGGAGAAGATTGGAGAATCTGTGAAGACAGAAATTGGGTTTGACTCGGAAGAAG 287 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 421 GGATTGGGGAGAAGATTGGAGAATCTGTGAAGACAGAAATTGGGTTTGACTCGGAAGAAG 480 Query: 286 CAAGTGGGAGAGTGAATGAGTATGTGGCTCGAGTTAAGGATAGTGTGCACAAGGGCCATC 227 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 481 CAAGTGGGAGAGTGAATGAGTATGTGGCTCGAGTTAAGGATAGTGTGCACAAGGGCCATC 540 Query: 226 ACGAGCTGACTCGCTTTAAGAATGAGACAGTGCCTTCGTTTATTGATTGGAACAAGTGGG 167 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 541 ACGAGCTGACTCGCTTTAAGAATGAGACAGTGCCTTCGTTTATTGATTGGAACAAGTGGG 600 Query: 166 AGCATTGGAAGGTAAGCTGGTTCAGCTTTTTAACGTAATTATCAAATTTCTGATTGATCA 107 ||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||| Sbjct: 601 AGCATTGGAAGGTAAGCTGGTTCAGCTTTTTAACGTAATTATCAAATTTCTGATTC-TCA 659 Query: 106 ATTGTAAATTCAATTGTAAATGGCTTC 80 | | | | | || | || ||| Sbjct: 660 AGTATTGTTAGACTTCTGCTTGTGTTC 686 >At1g56190 68414.t05913 phosphoglycerate kinase, putative similar to SP|P41758 Phosphoglycerate kinase, chloroplast precursor (EC 2.7.2.3) {Chlamydomonas reinhardtii}; contains Pfam profile PF00162: phosphoglycerate kinase Length = 2285 Plus Strand HSPs: Score = 521 (84.2 bits), Expect = 6.3e-15, P = 6.3e-15 Identities = 293/478 (61%), Positives = 293/478 (61%), Strand = Plus / Plus
Border_2 (3218_LB2D)
ATTGATCCCAATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATTGAGGGACAAACGTATGGTATTTATTTATTTTGTTGTTGATTTAACGGA
AGCATAAATTTTGGAGGAGACTACAACTACGCTTATTCTAGCGACATATACTAGTGTATTAGGTTGTTTCTTAATGAGGGAAGATGTATTGTATCAGGAC
ATCAGGAATTGGGACNGGTAAACCGAGTTGCTG:CCTTTGTTAAW
Start of BLAST Alignment: | NT 507 / Intron 1 of 18 |
First 15 bp Perfect Match: | NT 510 / Intron 1 of 18 |
BLAST Details: Query Sequence: 3218_LB2D Length: (244 letters) Database: Genes from AGI, Total Genome (DNA); Smallest Sum High Probability Sequences producing High-scoring Segment Pairs: Score P(N) N At1g79560 68414.t08478 FtsH protease, putative contains s... 923 6.2e-36 1 At5g33290 68418.t03637 exostosin family protein contains ... 167 0.10 1 At1g02300 68414.t00150 cathepsin B-like cysteine protease... 162 0.16 1 At1g59780 68414.t06165 disease resistance protein (CC-NBS... 162 0.17 1 >At1g79560 68414.t08478 FtsH protease, putative contains similarity to chloroplast FtsH protease GI:5804782 from [Nicotiana tabacum] Length = 6213 Plus Strand HSPs: Score = 923 (144.5 bits), Expect = 6.2e-36, P = 6.2e-36 Identities = 192/198 (96%), Positives = 193/198 (97%), Strand = Plus / Plus Query: 47 AATATTGAGGGACAAACGTATGGTATTTATTTATTTTGTTGTTGATTTAACGGAAGCATA 106 || ||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 690 AACATTGAGGGACAAACGTATGGTATTTATTTATTTTGTTGTTGATTTAACGGAAGCATA 749 Query: 107 AATTTTGGAGGAGACTACAACTACGCTTATTCTAGCGACATATACTAGTGTATTAGGTTG 166 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 750 AATTTTGGAGGAGACTACAACTACGCTTATTCTAGCGACATATACTAGTGTATTAGGTTG 809 Query: 167 TTTCTTAATGAGGGAAGATGTATTGTATCAGGACATCAGGAATTGGGACNGGTAAACCGA 226 ||||||||||||||||||||||||||||||||||||||||||||||||| ||||||| || Sbjct: 810 TTTCTTAATGAGGGAAGATGTATTGTATCAGGACATCAGGAATTGGGAC-GGTAAAC-GA 867 Query: 227 GTTGCTGCCTTTGTTAAW 244 ||||||||||| ||| |+ Sbjct: 868 GTTGCTGCCTT-GTTCAT 884 >At5g33290 68418.t03637 exostosin family protein contains Pfam profile: PF03016 Exostosin family Length = 3765 Plus Strand HSPs: Score = 167 (31.1 bits), Expect = 0.11, P = 0.10 Identities = 111/181 (61%), Positives = 111/181 (61%), Strand = Plus / Plus