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At5g27720 SYNGENTA 32187

Genomic Sequence (from TAIR)
taacaatatatactaaaacagataatattataaagttaagggactcgagtgcaaaactcaaaaaagttcccattgacctaaaactaaaagaccaccgatt
cattagaaatccatccctctcactgtcaaaacccgagaagcagaaaaaagcaaaaaacagatttcgaagtttcgacgcctctctattcttcgatctcttt
catatttgaatcgttcaccttcttgccgatcttcggagtatattacaaggtttctcccttttctctcagattttttttagtagctgaattagggtttttg
ttccgttttcgctattgcttcttccgcatcttataaagggtttaatgttatacttctctgatgtcatcaggttcttaacggatctctaaatcttctgatt
taagaagATGgtaagttactagacaaacttagggtttttttccttctttcttgtttgcgttaaagtttcaatttttattaactgaaagctgttgttttga
ttttatagCTTCCTCTATCGCTGCTTAAAACTGCTCAAGGGCATCCTATGgtgagtaaaatttcgacccttgattacaattttgttatatatgaagtgaa
gctgagtttaagtgagcattgcattagggatctaaaggaacttccttttactgtaattttgaatcttgttttgttttggtagCTCGTGGAGCTTAAGAAT
GGAGAGACATACAATGGGCATTTGGTGAATTGTGATACTTGGATGAACATCCATCTGCGTGAAGTCATATGTACATCAAAGgttatcttatgttttcata
tgttgatacaaatatactttgtttagacttcgtgatgaaattttgtgtagctgacactcatatattttgctgcttgattactctagGACGGAGATAGATT
TTGGAGGATGCCGGAATGTTATATCCGTGGTAACACAATCAAGTACCTTCGAGTTCCGGATGAGgtaatttttttttgtgtgtgtgtgtatatatctct
tgcttgatctagttttttctttgttcccctctctctggaatgatgatgcttagccaaatcagatatgtgctccaagaggcatttttctgtggaaccttgt
tcagcagatggtgtgcctcttgttgtagtgcgtcttagacgatatcacagggaatggttattgctgaccagaatgttgacccacttttatggactttgct
gtgttgtgtttctgatgcgatttttctctattgtttctactttatttcattccctctaaaatcaaattatattcacataccataaacgatatcttcatct
agaaggacagtcatatgctgtttctatttatgttcttactgctgcaacctagattgagttaactgacttaaattgatttcttctctcttcagGTGATTGA
TAAAGTACAGGAGGAGAAGACACGCACAGgtatagtattactcatgtctatattgtacctttactgagatatgcttcgagttttctgaggtgttaatatt
ttgccagagtagacattgagaatacttcaggtgttaataacgatggttgaatatgtgcagATAGAAAACCACCAGGTGTTGGACGTGGAAGAGGACGTGG
TGTGGATGATGGAGGAGCCAGAGGCAGAGGCAGAGGAACTTCAATGGGGAAGATGGGTGGCAACAGAGgtaaaaaatacacaaacaaagcttgtcacaaa
actctgattttactagaatacttcaaatatctgtgtcttgccacaatgggatttaagctaaaactctaatatgttatgaaaaacagGAGCAGGTCGCGGC
CGTGGTTGAtgcaacaagttttaaagactcgctgccaaatctgctaacttcccattgtggagaaaaccaaaagttacataagaagttggtctgttgttgg
ttttctattaagtatgtaagggatagggagtgtgtttttcgattctccttgtgatcttttcgaaaaatgaaatttgtcttttgcctttttgtggtatatg
tgaaattccaattgttttcatttgcgcttaaaaatgaatctttcaggtcttgaatttatataattgttagagaatttatacaattaaccaacgacagggt
ttttataagatctttggtccatcttacccttttattatttttctttttttcctatctattctttttttcctctttcaccggattttctccatcactcgtc
ggcatgacatataagattagatatgatttaaaaaacgcatgtatttcaaagacaaaacatgctcttaagtctttacattgacttgtaaatttttgtcaag
ctatctaacaaaatctcatattttttttttggaacagattcatatttttgtcaagatttcatgttatataaacaaattacatgtcttttatccaatatat
cattagtatttcataaaataactatgttttaatgtaataattatacatatctgtcaatatttcacgttatgtaaggagtcatatgtatttcttcaaatat
catgaaaattacatatttttcttcactgtatttttacaattgaattttcctcttctcaaatctatcggaagtcttattgtaatattagaaattgataaag
atattttcgtcaatatacaccgtctaacgtgtgaatccatagttcttttctaacaaaaactaaacttatgattaatatctttatttaatctcagaatttg
gctatctagtaataacaacctaattattatctgagtaaatccctagaaaaaactagtagtaatggaatttgcatgagaatatgagtattttggtcattta
ttttatttttctcaatgtaaacttaaatatttatgggtaaacaaaaaataaaatcatagaagatgatataattaaagatttgcgaacgaaaatctcataa
tcaaatctctctcttctgacacacgaaataatgacgataataaccctatcccgacacaatctctgacataaaatcaaagcatttaatgctcaaaatctct
ataaaaaaattcatgcatcagaatagttagcagataacgtattatcaggataacatgataacaaatatctcggtaatatttattaggttaacatgataa
caaatatttatatcaataagtttataattatgtcatggtaagtcattttgtatcgcgataagttataattgatgtgatatatatagatatttaccatctt

Border_1 (32187_LB1D)

CTTCTTGCCGATCTTCGGAGTATATTACAAGGTTTCTCCCTTTTCTCTCAGATTTTTTTTAGTAGCTGAATTAGGGTTTTTGTTCCGTTTTCGCTATTGC
TTCTTCCGCATCTTATAAAGGGTTTAATGTTATACTTCTCTGATGTCATCAGGTTCTTAACGGATCTCTAAATCTTCTGATTTAAGAAGATGGTAAGTTA
CTAGACAAACTTAGGGTTTTTTTCCTTCTTTCTTGTTTGCGTTAAAGTTTCAATTTTTATTAACTGAAAGCTGTTGTTTTGATTTTATAGCTTCCTCTAT
CGCTGCTTAAAACTGCTCAAGGGCATCCTATGGTGAGTAAAATTTCGACCCTTGATTACAATTTTGTTATATATGAAGTGAAGCTGAGTTTAAGTGAGCA
TTGCATTAGGGATCTAAAGGAACTTCCTTTTACTGTAATTTTGAATCTTGTTTTGTTTTGGTAGCTCGTGGAGCTTAAGAATGGAGAGACATACAATGGG
CATTTGGTGAATTGTGATACTTGGATGAACATCCATCTGCGTGAAGTCATATGTACATCAAAGGTTATCTTATGTTTTCATATGTTGATACAAATATACT
TTGTTTAGACTTCGTGATGAAATTTTGTGTAGCTGACACTCATATATTTTGCTGCTTGATTACTCTAGGACGGAGATAGATTTTGGAGGATGCCGGA

Start of BLAST Alignment:NT 508 / Exon 5 of 8 (Intron 1 in 5’ UTR)
First 15 bp Perfect Match:NT 508 / Exon 5 of 8 (Intron 1 in 5’ UTR)
Reverse Complement
BLAST Details:
Query Sequence: 32187_LB1D 
Length: (697 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At5g27720 68418.t03025 small nuclear ribonucleoprotein, p...  3429  1.8e-150  1
At5g56520 68418.t06546 expressed protein                       212  0.16      1
At5g25360 68418.t02731 expressed protein                       214  0.22      1
At5g50920 68418.t05867 ATP-dependent Clp protease ATP-bin...   213  0.25      1


>At5g27720 68418.t03025 small nuclear ribonucleoprotein, putative / snRNP,
           putative / Sm protein, putative similar to SWISS-PROT:Q9QXA5 U6
           snRNA-associated Sm-like protein LSm4 [Mus musculus]
        Length = 1998

  Plus Strand HSPs:

 Score = 3429 (520.5 bits), Expect = 1.8e-150, P = 1.8e-150
 Identities = 689/697 (98%), Positives = 689/697 (98%), Strand = Plus / Plus

Query:     1 CTTCTTGCCGATCTTCGGAGTATATTACAAGGTTTCTCCCTTTTCTCTCAGANNNNNNNN 60
             ||||||||||||||||||||||||||||||||||||||||||||||||||||        
Sbjct:   141 CTTCTTGCCGATCTTCGGAGTATATTACAAGGTTTCTCCCTTTTCTCTCAGATTTTTTTT 200

Query:    61 AGTAGCTGAATTAGGGTTTTTGTTCCGTTTTCGCTATTGCTTCTTCCGCATCTTATAAAG 120
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   201 AGTAGCTGAATTAGGGTTTTTGTTCCGTTTTCGCTATTGCTTCTTCCGCATCTTATAAAG 260

Query:   121 GGTTTAATGTTATACTTCTCTGATGTCATCAGGTTCTTAACGGATCTCTAAATCTTCTGA 180
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   261 GGTTTAATGTTATACTTCTCTGATGTCATCAGGTTCTTAACGGATCTCTAAATCTTCTGA 320

Query:   181 TTTAAGAAGATGGTAAGTTACTAGACAAACTTAGGGTTTTTTTCCTTCTTTCTTGTTTGC 240
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   321 TTTAAGAAGATGGTAAGTTACTAGACAAACTTAGGGTTTTTTTCCTTCTTTCTTGTTTGC 380

Query:   241 GTTAAAGTTTCAATTTTTATTAACTGAAAGCTGTTGTTTTGATTTTATAGCTTCCTCTAT 300
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   381 GTTAAAGTTTCAATTTTTATTAACTGAAAGCTGTTGTTTTGATTTTATAGCTTCCTCTAT 440

Query:   301 CGCTGCTTAAAACTGCTCAAGGGCATCCTATGGTGAGTAAAATTTCGACCCTTGATTACA 360
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   441 CGCTGCTTAAAACTGCTCAAGGGCATCCTATGGTGAGTAAAATTTCGACCCTTGATTACA 500

Query:   361 ATTTTGTTATATATGAAGTGAAGCTGAGTTTAAGTGAGCATTGCATTAGGGATCTAAAGG 420
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   501 ATTTTGTTATATATGAAGTGAAGCTGAGTTTAAGTGAGCATTGCATTAGGGATCTAAAGG 560

Query:   421 AACTTCCTTTTACTGTAATTTTGAATCTTGTTTTGTTTTGGTAGCTCGTGGAGCTTAAGA 480
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   561 AACTTCCTTTTACTGTAATTTTGAATCTTGTTTTGTTTTGGTAGCTCGTGGAGCTTAAGA 620

Query:   481 ATGGAGAGACATACAATGGGCATTTGGTGAATTGTGATACTTGGATGAACATCCATCTGC 540
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   621 ATGGAGAGACATACAATGGGCATTTGGTGAATTGTGATACTTGGATGAACATCCATCTGC 680

Query:   541 GTGAAGTCATATGTACATCAAAGGTTATCTTATGTTTTCATATGTTGATACAAATATACT 600
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   681 GTGAAGTCATATGTACATCAAAGGTTATCTTATGTTTTCATATGTTGATACAAATATACT 740

Query:   601 TTGTTTAGACTTCGTGATGAAATTTTGTGTAGCTGACACTCATATATTTTGCTGCTTGAT 660
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   741 TTGTTTAGACTTCGTGATGAAATTTTGTGTAGCTGACACTCATATATTTTGCTGCTTGAT 800

Query:   661 TACTCTAGGACGGAGATAGATTTTGGAGGATGCCGGA 697
             |||||||||||||||||||||||||||||||||||||
Sbjct:   801 TACTCTAGGACGGAGATAGATTTTGGAGGATGCCGGA 837


>At5g56520 68418.t06546 expressed protein
        Length = 745

  Plus Strand HSPs:

 Score = 212 (37.9 bits), Expect = 0.18, P = 0.16
 Identities = 214/379 (56%), Positives = 214/379 (56%), Strand = Plus / Plus


Border_2 (32187_RBU)

CTCGGTAATATTTATTAGGTTAACATGATAACAAATATTTATATCAATAAGTTTATAATTATGTCATGGTAAGTCATTTTGTATCGCGATAAGTTATAAT
TGATGTGATATATATAGATATTTACCATCTTAACTCATTTATTATATCATAATAAAAAATTAACGAGTTACTATAACGTACAAAATATGAATTTCATCCA
ATAACATTATTATTATCACTACCTCTAGATAAAGTTTTAGTTAACTAGGTAATTTAATTGTTAACCAATTTGAATCTGAACAAAAACTACCATTGCGTAA
TCTTCAACCACCACATCTCCGCCGTATTGTTCTCACCCCATCATTTCAGAACCATTTTTTTCCTCTACCGCAAACCCACACCCTAATCGATTTAGAACTA
GATCTTTAAACGATTGAGAGAAACTGCCGGTAATTCGCGAAACTTATTTGGATTTATCGACCAGAACCACAGAGCTTTGAACGGAGACTGGAACTGAATA
TCGGAAAAAAGAAAAACAGAGTTAAGTTGAATCGAATCTATATGAGAGATATTATGTCTCTCGTCTCTGACACACGTTACTGTGTATCTTATATATTACT
TTAGACTGGATAGACTGTGTAGGGGCTTATAGGTCATATTTTGTGTACAAAAACCATATAAACAATGTCGAAAATATGTCAGTTA

Start of BLAST Alignment:NT > 1259 / Downstream
First 15 bp Perfect Match:NT > 1259 / Downstream
BLAST Details:
Query Sequence: 32187_RBU 
Length: (685 letters) 
Database: TIGR AGI BAC Sequences (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

T1G16 67960                                                   3416  3.9e-148  1
T24H24 67057                                                  1557  3.8e-64   1
F5M15 60115                                                   1277  1.7e-51   1
F2D10 61258                                                   1277  1.7e-51   1


T1G16 67960
        Length = 93,672

  Plus Strand HSPs:

 Score = 3416 (518.6 bits), Expect = 3.9e-148, P = 3.9e-148
 Identities = 684/685 (99%), Positives = 684/685 (99%), Strand = Plus / Plus

Query:     1 CTCGGTAATATTTATTAGGTTAACATGATAACAAATATTTATATCAATAAGTTTATAATT 60
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 38818 CTCGGTAATATTTATTAGGTTAACATGATAACAAATATTTATATCAATAAGTTTATAATT 38877

Query:    61 ATGTCATGGTAAGTCATTTTGTATCGCGATAAGTTATAATTGATGTGATATATATAGATA 120
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 38878 ATGTCATGGTAAGTCATTTTGTATCGCGATAAGTTATAATTGATGTGATATATATAGATA 38937

Query:   121 TTTACCATCTTAACTCATTTATTATATCATAATAAAAAATTAACGAGTTACTATAACGTA 180
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 38938 TTTACCATCTTAACTCATTTATTATATCATAATAAAAAATTAACGAGTTACTATAACGTA 38997

Query:   181 CAAAATATGAATTTCATCCAATAACATTATTATTATCACTACCTCTAGATAAAGTTTTAG 240
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 38998 CAAAATATGAATTTCATCCAATAACATTATTATTATCACTACCTCTAGATAAAGTTTTAG 39057

Query:   241 TTAACTAGGTAATTTAATTGTTAACCAATTTGAATCTGAACAAAAACTACCATTGCGTAA 300
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 39058 TTAACTAGGTAATTTAATTGTTAACCAATTTGAATCTGAACAAAAACTACCATTGCGTAA 39117

Query:   301 TCTTCAACCACCACATCTCCGCCGTATTGTTCTCACCCCATCATTTCAGAACCATTTTTT 360
             |||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct: 39118 TCTTCAACCACCACATCTCCGCCGTATTCTTCTCACCCCATCATTTCAGAACCATTTTTT 39177

Query:   361 TCCTCTACCGCAAACCCACACCCTAATCGATTTAGAACTAGATCTTTAAACGATTGAGAG 420
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 39178 TCCTCTACCGCAAACCCACACCCTAATCGATTTAGAACTAGATCTTTAAACGATTGAGAG 39237

Query:   421 AAACTGCCGGTAATTCGCGAAACTTATTTGGATTTATCGACCAGAACCACAGAGCTTTGA 480
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 39238 AAACTGCCGGTAATTCGCGAAACTTATTTGGATTTATCGACCAGAACCACAGAGCTTTGA 39297

Query:   481 ACGGAGACTGGAACTGAATATCGGAAAAAAGAAAAACAGAGTTAAGTTGAATCGAATCTA 540
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 39298 ACGGAGACTGGAACTGAATATCGGAAAAAAGAAAAACAGAGTTAAGTTGAATCGAATCTA 39357

Query:   541 TATGAGAGATATTATGTCTCTCGTCTCTGACACACGTTACTGTGTATCTTATATATTACT 600
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 39358 TATGAGAGATATTATGTCTCTCGTCTCTGACACACGTTACTGTGTATCTTATATATTACT 39417

Query:   601 TTAGACTGGATAGACTGTGTAGGGGCTTATAGGTCATATTTTGTGTACAAAAACCATATA 660
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 39418 TTAGACTGGATAGACTGTGTAGGGGCTTATAGGTCATATTTTGTGTACAAAAACCATATA 39477

Query:   661 AACAATGTCGAAAATATGTCAGTTA 685
             |||||||||||||||||||||||||
Sbjct: 39478 AACAATGTCGAAAATATGTCAGTTA 39502


T24H24 67057
        Length = 88,848

  Plus Strand HSPs:

 Score = 1557 (239.7 bits), Expect = 3.8e-64, P = 3.8e-64
 Identities = 347/384 (90%), Positives = 347/384 (90%), Strand = Plus / Plus

Query:    13 TATTAGGTTAACATGATAACAAATATTTATATCAATAAGTTTATAATTATGTCATGGTAA 72
             || ||  |||  | | ||  |||||||||||||||||||||||||||||||||||| |||
Sbjct: 45303 TAATAC-TTATTAGGTTACAAAATATTTATATCAATAAGTTTATAATTATGTCATGCTAA 45361

Query:    73 GTCATTTTGTATCGCGATAAGTTATAATTGATGTGATATATATAGATATTTACCATCTTA 132
             ||||||||||||||||||||||||||| | ||||||||||| ||  |||||||| |||||
Sbjct: 45362 GTCATTTTGTATCGCGATAAGTTATAACTTATGTGATATATTTA--TATTTACCTTCTTA 45419

Query:   133 ACTCATTTATTATATCATAATAAAAAATTAACGAGTTACTATAACGTACAAAATATGAAT 192
             ||||| |||||||||||||  ||||||||||| |||||||||||| | ||||| || |||
Sbjct: 45420 ACTCACTTATTATATCATATAAAAAAATTAACAAGTTACTATAACATGCAAAAAATAAAT 45479

Query:   193 TTCATCCAATAACATTATTATTATCACTACCTCTAGATAAAGTTTTAGTTAACTAGGTAA 252
             |||||||||||| |||| ||||||||||||||||||||||| ||||||||| || |||||
Sbjct: 45480 TTCATCCAATAAGATTAATATTATCACTACCTCTAGATAAATTTTTAGTTACCTGGGTAA 45539

Query:   253 TTTAATTGTTAACCAATTTGAATCTGAACAAAAACTACCATTG-CGTAATCTTCAACCAC 311
             ||| ||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
Sbjct: 45540 TTTGATTGTTAACCAATTTGAATCTGAACAAAAACTACCATTGTCGTAATCTTCAACCAC 45599

Query:   312 CACATCTCCGCCGTATTGTTCTCACCCCATCATTTCAGAACCATTTTTTTCCTCTACCGC 371
             ||||| ||||||||||| ||||||||||||||||||||||||||||||||||||||||||
Sbjct: 45600 CACATTTCCGCCGTATTCTTCTCACCCCATCATTTCAGAACCATTTTTTTCCTCTACCGC 45659

Query:   372 AAACCCACACCCTAATCGATTTAG 395
             ||||||||||| ||||  |||| |
Sbjct: 45660 AAACCCACACCATAATT-ATTTGG 45682