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At5g22370 SYNGENTA 35154

Genomic Sequence (from TAIR)
cttcgccttgaatcgagtcttcgacgagtctccggctgcgagtttctcttgctccggcaaacagacctgtcattgcttctctctccggctaactacacag
gcgagcttctctatttattttcttctgtgtctccagttgcttctgatataatgaatatgctcttgaagcaaattgtttctctgcttctctttctccagtg
aattgtttgttgtattataaacgaggtttaggaattgggttaataatattggaggattgtgtttgtattctgtgtccgacgaacaaatgacatttccgat
ttctattgtgctacgagctcagtgtttagaagggttttgatgttctctattgaatattcatgttatgttagtttgatgattagatgattttgctttgcaa
ttactgtgtttttttgtctgactggctctgatattatacaacgtgtttgactccgattggtatcaatcttcttcttcgtcttcagaagcATGGTGTTTGG
ACAAGTAGTAATAGGTCCTCCAGGATCGGGAAAGACCACTTATTGCAATGGAATGTCTCAGTTCCTCTCTCTAATGGGCAGgtttcatgttctttctctt
ctcttctctttttggatcaaagaatgaagcttacttggcttttgtatctgtaattctgtttgtattgattgtttcgctgtccctcgcattgtaaagtt
tacaagttgcaaactttagtaaataatagtgttgaattccagaaatttgaattgtctgacttttgagcagagtcattgtgaaatgttgttgccttgtc
tttgtttttggtagagagatttgcagattcgtgagaacttctctgtaagtaaattctcttaacgttgagttactattttgttgttgttgcagGAAGGTTG
CTATTGTTAATCTGGATCCTGCAAATGATGCATTACCgtatccttttcaagccaaatacaacaaaattgttaaaaattgcaggtgtatgttgtttcttga
cattgattttagTTATGAGTGTGGTGTGAATATAGAAGAATTGATCAAGTTAGAAGATGTTATGTCGGAACACTCGCTTGGTCCTAATGGAGgtgaattt
tgccatcttgattttcccttttcttcttacttttgattttaatctttggtcaaattaacaaagaaactttctaatgtgacagGTCTTGTATATTGTATGG
AGTACTTGGAGAAAAACATTGACTGGCTGGAATCTAAACTAAAGCCTCTTCTGAAGGgttagcaacgatatgtcccttcttcttcttcttcttttcgtgg
tcataatatctctttcttaaagtaaaacgttttgaattgtttgcagATCATTACATTCTCTTTGATTTTCCTGGCCAAGTGGAATTGTTCTTCATTCATG
ACAGTACCAAGAATGTTCTCACGAAGCTGATTAAATCATTGAACCTTAGAgtatgtgctttacttcacttattactaacttgctaaatgaaccttactta
ggagtctacatatctgatgaatttctttgttctgcagTTAACTGCTGTGCAACTAATTGATTCCCATCTATGTTGTGATCCCGGGAACTACGTAAGTTCG
CTACTTCTCTCCTTATCCACAATGCTTCACATGGAACTCCCACATGTCAATGTATTGTCTAAAATCGATCTGATTGGAAGCTACGGGAAGCTAGgtatta
ataatctatctatggttttgttctcataacatcgaattttcaaatgtatactgtggactcactaactaggatctctgtgcatgtagCTTTCAATTTAGAT
TTCTATACCGATGTTCAAGACTTGTCATACTTGGAGCACCATCTTAGTCAAGATCCTCGCTCTGCTAAGTACAGgttcgatgtaaacatcatctgaatac
acattgaacggttaataattatccatttagaagcatgctcaatatctcatgagcttgatgtttctcattgcagAAAACTAACAAAAGAGCTATGTAGTGT
CATTGAAGATTACAGTCTTGTTAATTTTACAACCTTGGATATTCAGgtttgcgatttcctttacctctctctctctttctctctgtacaagcatcttttc
gcaattttcttttttgttttcagGATAAAGAAAGTGTTGGGGATCTAGTAAAGCTCATCGACAAGAGCAATGGATACATATTTGCCGGCATTGATGCAAG
TGTGGTTGAATACAGCAAGATTGCAATTGGTCAAACTGATTGGGATTATAACAGATATCCTTTTTTAGCATTTTGGATTTCTTAAccattacttctctca
ataactcttgagtttagttttacgtctactacaaaagaattgtttctttgacgatagtcttcacagtcgcagctgtacaggagaagtacatggaagatga
ggaaatacaagactgagaacagtgcttgaaactttatatagaagagagctggtttaaaatatctctgaaccaaaccatttgacggtttgtatgaaccttt
ttgtgtatttttgagttcggtttaagtctaaacaacattgatttacacaacttcctggtttggttcactggattatgctaattttccagacgactaactt
tctgccaaattttgtctttagacttttgagtttctgaatcacacgtgcccatgactccaacattctctagaaaacatctcaaatgtcactgtctaggtca
ttta

Border_1 (35154_RBD)

ATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGA
TTACGCCAAGCTCGAAATTAACCCTCACTAAAGGGAACAAAAGCTGGAGCTCGCTTGAGCTTGGATCAGATTGTCGTTTCCCGCCTTCAGTTTAAAAATT
GACGCTGTTTGTATTATTTCACAATCAATGCGAGGGACAGCGAAACAATCAATACAAACAGAATTACAGATACAAAAGCCAAGTAAGCTTCATTCTTTGA
TCCAAAAAGAGAAGAGAAGAGAAAGAACATGAAACCTGCCCATTAGAGAGAGGAACTGAGACATTCCATTGCAATAAGTGGTCTTTCCCGATCCTGGAGG
ACCTATTACTACTTGTCCAAACACCATGCTTCTGAAGACGAAGAAGAAGATTGATACCAATCGGAGTCAAACACGTTGTATAATATCAGAGCCAGTCAGA
CAAAAAAACACAGTAATTGCAAAGCAAAATCATCTAATCATCAAACTAACATAACATGAATATTCAATAGAGAACATCAAAACCCTTCTAAACACTGAGC
TCGTAGCACAATAGAAATCGGAAATGTCATTTGTTCGTCGGACACAGAATACAAACACAATCCTCCAATATTATTAACCCAATTCCTAAACCTCGTTTAT
AATACAACAAACAATTCACTGGAGAAAGAGAAGCAGAGAAACAATTTGCTTCATGAGCATATTCATTATATCAGACGCAACTGGAGACACANNAGAANAT
AAATAGAGANGCTCGCCTGTGTAGTTAGCCGGAGAGAGAAGCAATGACC

Start of BLAST Alignment:NT 254 / Intron 2 of 9 (Intron 1 at 5’ UTR)
First 15 bp Perfect Match:NT 202 / Intron 2 of 9 (Intron 1 at 5’ UTR)
BLAST Details:
Query Sequence: 35154_RBD 
Length: (849 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At5g22370 68418.t02367 ATP-binding family protein contain...  3077  1.3e-134  1
At1g78380 68414.t08352 glutathione S-transferase, putativ...   218  0.18      1
At2g19260 68415.t02133 ELM2 domain-containing protein / P...   210  0.44      1
At5g48720 68418.t05599 expressed protein                       204  0.63      1


>At5g22370 68418.t02367 ATP-binding family protein contains Pfam domain,
           PF03029: Conserved hypothetical ATP binding protein
        Length = 2268

  Minus Strand HSPs:

 Score = 3077 (467.7 bits), Expect = 1.3e-134, P = 1.3e-134
 Identities = 643/675 (95%), Positives = 643/675 (95%), Strand = Minus / Plus

Query:   848 GTCATTGCTTCTCTCTCCGGCTAACTACACAGGCGAGCNTCTCTATTTATNTTCTNNTGT 789
             |||||||||||||||||||||||||||||||||||||| ||||||||||| ||||  |||
Sbjct:    56 GTCATTGCTTCTCTCTCCGGCTAACTACACAGGCGAGCTTCTCTATTTATTTTCTTCTGT 115

Query:   788 GTCTCCAGTTGCGTCTGATATAATGAATATGCTCATGAAGCAAATTGTTTCTCTGCTTCT 729
             |||||||||||| ||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct:   116 GTCTCCAGTTGCTTCTGATATAATGAATATGCTCTTGAAGCAAATTGTTTCTCTGCTTCT 175

Query:   728 CTTTCTCCAGTGAATTGTTTGTTGTATTATAAACGAGGTTTAGGAATTGGGTTAATAATA 669
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   176 CTTTCTCCAGTGAATTGTTTGTTGTATTATAAACGAGGTTTAGGAATTGGGTTAATAATA 235

Query:   668 TTGGAGGATTGTGTTTGTATTCTGTGTCCGACGAACAAATGACATTTCCGATTTCTATTG 609
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   236 TTGGAGGATTGTGTTTGTATTCTGTGTCCGACGAACAAATGACATTTCCGATTTCTATTG 295

Query:   608 TGCTACGAGCTCAGTGTTTAGAAGGGTTTTGATGTTCTCTATTGAATATTCATGTTATGT 549
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   296 TGCTACGAGCTCAGTGTTTAGAAGGGTTTTGATGTTCTCTATTGAATATTCATGTTATGT 355

Query:   548 TAGTTTGATGATTAGATGATTTTGCTTTGCAATTACTGTGTTTTTTTGTCTGACTGGCTC 489
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   356 TAGTTTGATGATTAGATGATTTTGCTTTGCAATTACTGTGTTTTTTTGTCTGACTGGCTC 415

Query:   488 TGATATTATACAACGTGTTTGACTCCGATTGGTATCAATCTTCTTCTTCGTCTTCAGAAG 429
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   416 TGATATTATACAACGTGTTTGACTCCGATTGGTATCAATCTTCTTCTTCGTCTTCAGAAG 475

Query:   428 CATGGTGTTTGGACAAGTAGTAATAGGTCCTCCAGGATCGGGAAAGACCACTTATTGCAA 369
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   476 CATGGTGTTTGGACAAGTAGTAATAGGTCCTCCAGGATCGGGAAAGACCACTTATTGCAA 535

Query:   368 TGGAATGTCTCAGTTCCTCTCTCTAATGGGCAGGTTTCATGTTCTTTCTCTTCTCTTCTC 309
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   536 TGGAATGTCTCAGTTCCTCTCTCTAATGGGCAGGTTTCATGTTCTTTCTCTTCTCTTCTC 595

Query:   308 TTTTTGGATCAAAGAATGAAGCTTACTTGGCTTTTGTATCTGTAATTCTGTTTGTATTGA 249
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   596 TTTTTGGATCAAAGAATGAAGCTTACTTGGCTTTTGTATCTGTAATTCTGTTTGTATTGA 655

Query:   248 TTGTTTCGCTGTCCCTCGCATTG-ATTGTGAAATAATA-CAAACAGC-GTCAATTTTTAA 192
             ||||||||||||||||||||||| |  ||  |  | |  |||||    || |||  |   
Sbjct:   656 TTGTTTCGCTGTCCCTCGCATTGTAAAGTTTACAAGTTGCAAACTTTAGTAAATAATAGT 715

Query:   191 ACTGAAGGCGGGAAA 177
               ||||  |  ||||
Sbjct:   716 GTTGAATTCCAGAAA 730


>At1g78380 68414.t08352 glutathione S-transferase, putative similar to
           glutathione transferase GI:2853219 from [Carica papaya]
        Length = 1494

  Minus Strand HSPs:

 Score = 218 (38.8 bits), Expect = 0.20, P = 0.18
 Identities = 240/421 (57%), Positives = 240/421 (57%), Strand = Minus / Plus


Border_2 (35154_LBU)

GCCTTTTCAGAAATGGATAAATAGCCTTGCTTCCTATTATATCTTCCCAAATTACCAATACATTACACTAGCATCTGAATTTCATAACCAATCTCGATAC
ACCAAATCGAATTCAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTACACCACAATATAT
CCTGAAATAATACAAACAGCGATTATCTGTTTGTAATTTGTCTAAGCGTCATTTGCAAACTTTAGTAAATAATAGTGTTGAATTCCAGAAATTTGAATTG
TCTGACTTTTGAGCAGAGTCATTGTGAAATGTTGTTGCCTTGTCTTTGTTTTTGGTAGAGAGATTTGCAGATTCGTGAGAACTTCTCTGTAAGTAAATTC
TCTTAACGTTGAGTTACTATTTTGTTGTTGTTGCAGGAAGGTTGCTATTGTTAATCTGGATCCTGCAAATGATGCATTACCGTATCCTTTTCAAGCCAAA
TACAACAAAATTGTTAAAAATTGCAGGTGTATGTTGTTTCTTGACATTGATTTTAGTTATGAGTGTGGTGTGAATATAGAAGAATTGATCAAGTTAGAAG
ATGTTATGTCGGAACACTCGCTTGGTCCTAATGGAGGTGAATTTTGCCATCTTGATTTTCCCTTTTCTTCTTACTTTTGATTTTAATCTTTGGTCAAATT
AACAAAGAAACTTTCTAATGTGACAGGTCTTGTATATTGTATGGAGTACTTGGAGAAAAACATTGACCTGGCTGGAATCTAAACTAAAGCCTCTTCTGAA
GGGTTAGCAACGATATGTCCCTTCTTCTTCTTCTTCT

Start of BLAST Alignment:NT 199 / Intron 2 of 9 (Intron 1 at 5’ UTR)
First 15 bp Perfect Match:NT 215 / Intron 2 of 9 (Intron 1 at 5’ UTR)
BLAST Details:
Query Sequence: 35154_LBU 
Length: (837 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At5g22370 68418.t02367 ATP-binding family protein contain...  2914  2.9e-127  1
At2g41490 68415.t04704 UDP-GlcNAc:dolichol phosphate N-ac...   241  0.018     1
At5g30450 68418.t03415 CACTA-like transposase family (Ptt...   240  0.021     1
At3g29180 68416.t03326 expressed protein                       237  0.030     1


>At5g22370 68418.t02367 ATP-binding family protein contains Pfam domain,
           PF03029: Conserved hypothetical ATP binding protein
        Length = 2268

  Plus Strand HSPs:

 Score = 2914 (443.3 bits), Expect = 2.9e-127, P = 2.9e-127
 Identities = 594/602 (98%), Positives = 594/602 (98%), Strand = Plus / Plus

Query:   238 TTGTCTAAGCGT-CAT-TTGCAAACTTTAGTAAATAATAGTGTTGAATTCCAGAAATTTG 295
             ||||  |||  | ||  |||||||||||||||||||||||||||||||||||||||||||
Sbjct:   676 TTGTA-AAGTTTACAAGTTGCAAACTTTAGTAAATAATAGTGTTGAATTCCAGAAATTTG 734

Query:   296 AATTGTCTGACTTTTGAGCAGAGTCATTGTGAAATGTTGTTGCCTTGTCTTTGTTTTTGG 355
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   735 AATTGTCTGACTTTTGAGCAGAGTCATTGTGAAATGTTGTTGCCTTGTCTTTGTTTTTGG 794

Query:   356 TAGAGAGATTTGCAGATTCGTGAGAACTTCTCTGTAAGTAAATTCTCTTAACGTTGAGTT 415
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   795 TAGAGAGATTTGCAGATTCGTGAGAACTTCTCTGTAAGTAAATTCTCTTAACGTTGAGTT 854

Query:   416 ACTATTTTGTTGTTGTTGCAGGAAGGTTGCTATTGTTAATCTGGATCCTGCAAATGATGC 475
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   855 ACTATTTTGTTGTTGTTGCAGGAAGGTTGCTATTGTTAATCTGGATCCTGCAAATGATGC 914

Query:   476 ATTACCGTATCCTTTTCAAGCCAAATACAACAAAATTGTTAAAAATTGCAGGTGTATGTT 535
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   915 ATTACCGTATCCTTTTCAAGCCAAATACAACAAAATTGTTAAAAATTGCAGGTGTATGTT 974

Query:   536 GTTTCTTGACATTGATTTTAGTTATGAGTGTGGTGTGAATATAGAAGAATTGATCAAGTT 595
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   975 GTTTCTTGACATTGATTTTAGTTATGAGTGTGGTGTGAATATAGAAGAATTGATCAAGTT 1034

Query:   596 AGAAGATGTTATGTCGGAACACTCGCTTGGTCCTAATGGAGGTGAATTTTGCCATCTTGA 655
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1035 AGAAGATGTTATGTCGGAACACTCGCTTGGTCCTAATGGAGGTGAATTTTGCCATCTTGA 1094

Query:   656 TTTTCCCTTTTCTTCTTACTTTTGATTTTAATCTTTGGTCAAATTAACAAAGAAACTTTC 715
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1095 TTTTCCCTTTTCTTCTTACTTTTGATTTTAATCTTTGGTCAAATTAACAAAGAAACTTTC 1154

Query:   716 TAATGTGACAGGTCTTGTATATTGTATGGAGTACTTGGAGAAAAACATTGACCTGGCTGG 775
             |||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
Sbjct:  1155 TAATGTGACAGGTCTTGTATATTGTATGGAGTACTTGGAGAAAAACATTGAC-TGGCTGG 1213

Query:   776 AATCTAAACTAAAGCCTCTTCTGAAGGGTTAGCAACGATATGTCCCTTCTTCTTCTTCTT 835
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1214 AATCTAAACTAAAGCCTCTTCTGAAGGGTTAGCAACGATATGTCCCTTCTTCTTCTTCTT 1273

Query:   836 CT 837
             ||
Sbjct:  1274 CT 1275


>At2g41490 68415.t04704 UDP-GlcNAc:dolichol phosphate
           N-acetylglucosamine-1-phosphate transferase identical to GI:5804772
        Length = 2517

  Plus Strand HSPs:

 Score = 241 (42.2 bits), Expect = 0.019, P = 0.018
 Identities = 225/386 (58%), Positives = 225/386 (58%), Strand = Plus / Plus