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At3g20070 SYNGENTA 35438

Genomic Sequence (from TAIR)

caatcaggattgtttataacagaggaaatttgtactaagttcccagcgaaactctcgtgaccttcatggcatgtacaattatggtcacaagtgtggacca
aaatagatcttttcatcaaaaaaaaaaatgtgtgaatccaatggataacttggttttcatattcttataattaagaacttaaaattaaataaaaagatag
aagaaaaatagtgctttgagaagaagaaaaaaaccgttgccctagaattaaaatccacttctcagtgtcataggcgtcgtcggtttgggaattttataaa
gttggtctcttttgaattcatcagtttcgtcattctccttcaaatctggaaaccaaagcgagtgtgttttgctctcttgaatcgaatttgtaagctcgtc
gattttgcttctttccgggtcgggtaatcaatttgatttgattcttcactcttctacagtgagcagaaaATGGAAGCTCTCTACGCCAAGCTCTACGACA
AATACACCAAACTCCAGgttttctgcaacatcttccgtttaattttcttgtcaatttctgagggtttctccggaatttcgcttttctgggttcgattttg
atttcgatctccttttttttcagAAGAAGAAATATTCCGAATACGATGAGATTAATAAGGAACAAGAAGAGAAATTCCTTACCTTTGTTTCAGgtaccaa
tttcgaaagattcgttctttgagtttgagttcctttgttccatacttgaattttagatatctctagttaggtttgatttcacattgcaattacaaacact
tggaatcgagaattataacaagagaccttttaagtctgtgaatacgttcttagtgtgtctgtcttttctctcttttgctatttgcagCCTCAGAGGAGTT
GATGGAACATTTGAGAGGTGAAAATCAGAGTTCTCTGGAGATGGTTGAGAAGTTGAGGAATGAAATCATTTCAATCAGgcatgtacttcaccttaccgtc
ttgattttcccagacattcttttttatttagagaagttggagccttgctaatgtaactataacaatcttgatccacagATCTGGCAGGGACGACAAGTTT
CTGGAGTGTCAAAAGCTTCTCATGGAAGAAGAACTAAAGAgtaagaaactatgttaattgatgatagttgctgctctattttcatgtgtcatggaatgta
gccttataattggacatgatatgtagtttagttaggaatttagctgagataatgtgaactgcttcatggagagtgatgaggacttggttataatactgtc
taggataaaatctttgttgtttttcttacagACAAATCACTTTCTGAAGAAGTTGTCAAGCTAAAAGAGCTGGTCCAAGAGGAACATCCTCGCAACTATG
AAGATCAAAGTGGAAAAAAACAAAAGAGAAAGACTCCTGAAAGTGCTCGAGTTACAACGAGAAGCATGATAAAACGTAGCAGACTGTCAGAAGATTTGGT
GGAAACGGATATGGTATCACCTGACATTAGCAAACATCATAAAGCAAAGgtaaaacagatcacctcttaacctttcagttctga◄gctga◄atgttgtgc
atcttgcttcacaaacccagtggatcataaaattagccttactgatactttttcttgcttttgtttcaagGAGCCTCTCTTGGTTTCTCAGCCACAATGC
TGCAGAACAACCTACGATGGATCAAGTAGTTCTGCTAGTTGTACATTTCAAGCTCTTGGCAAACACTTGCTAGGAATGAAATTATCAACTAATAATAAGG
GCAAACGCGCCTGCATTGTAGCCTCACACCCAACAACCGgtaatttgtttttcctgcatgatctatttactttgattcaaaagatcttttgagatcatca
gccaccagaaaagcaccaatgctgattttgtttccctgattattttaccataacagGTTTATCCTTCAGCCTAACTTTTATAAATAACCCAAATGGTGAA
GAATCTGAGCTGCTTTACAAGCCTGCATCACTCGGAACATTTCAAAGAGTGGCACCGGAATGGATGAGAGAAGTTATAAAGTTCAGCACAAGTATGTGTC
CCATC►TTCTTTGAAAGAGTCTCTCGAGTCATTAAGCTCAATTGTTGAtcggta►ccgtatgtacatctccctccccttatcttgtgtagtcatctctga
tggcttcttagtgtgatgtttctcaataggtttgaaaagctagggaagttatcattgtatctatagaaatgtgtctctctttaactttggaattagtttc
aaagatagtgtgtccttgtgatgaatcatattgtaacaagatcttgatttatcctttgctctcgataccctctcctcttgtagacctcataaatctctga
tctattgagcattttacatgattcaacattttacatatttttgtaacaaattttatcattggaaagattaaatagccgttacataaggtaggtgaatttt
caattcaaatgcatcaaaatagccgctataacctaagtgataacgttcggaaaatgctttaggataattcctttttattgcttttgaaaacgacgacgtt
tctt

Border_1 (35438_LB1U)


TGAATTTCATAACCAATCTCTGATACACCAAATCGAATTCAATTCGGCGTTAATTCAGTACATTAAAAACGTCC:GCAATGTGTTATTAAGTTGTCTAAG
CGTCAGAACTGAAAGGTTAAGAGGTGATCTGTTTTACCTTTGCTTTATGATGTTTGCTAATGTCAGGTGATACCATATCCGTTTCCACCAAATCTTCTGA
CAGTCTGCTA:CGTTTTATCATGC:TTCTCGTTGTAACYTCGAGCACT:TTCAGGAGTCTTTCTCTTTTGTTTTTTTCCACTTTGATCTTCATAGTTGCG
AGGATTGTTCCTANGCTAGTGTATCGAGATTGGTTATAAAATCCAGATGGC

Start of BLAST Alignment:	NT 1120 / Intron 5 of 6
First 15 bp Perfect Match:	NT 1115 / Intron 5 of 6

BLAST Details:
Query Sequence: 35438_LB1U 
Length: (347 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At3g20070 68416.t02267 expressed protein                      1011  2.0e-40   1
At5g53950 68418.t06229 no apical meristem (NAM) family pr...   183  0.40      1
At3g58460 68416.t06033 rhomboid family protein / ubiquiti...   183  0.43      1
At3g19040 68416.t02154 ubiquitin family protein / DNA-bin...   174  0.91      1


>At3g20070 68416.t02267 expressed protein
        Length = 2247

  Minus Strand HSPs:

 Score = 1011 (157.7 bits), Expect = 2.0e-40, P = 2.0e-40
 Identities = 209/213 (98%), Positives = 209/213 (98%), Strand = Minus / Plus

Query:   308 AGGAACAATCCTCGCAACTATGAAGATCAAAGTGGAAAAAAACAAAAGAGAAAGACTCCT 249
             ||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1204 AGGAACA-TCCTCGCAACTATGAAGATCAAAGTGGAAAAAAACAAAAGAGAAAGACTCCT 1262

Query:   248 GAAAGTGCTCGARGTTACAACGAGAAGCATGATAAAACGTAGCAGACTGTCAGAAGATTT 189
             |||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1263 GAAAGTGCTCGA-GTTACAACGAGAAGCATGATAAAACGTAGCAGACTGTCAGAAGATTT 1321

Query:   188 GGTGGAAACGGATATGGTATCACCTGACATTAGCAAACATCATAAAGCAAAGGTAAAACA 129
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1322 GGTGGAAACGGATATGGTATCACCTGACATTAGCAAACATCATAAAGCAAAGGTAAAACA 1381

Query:   128 GATCACCTCTTAACCTTTCAGTTCTGACGCTTA 96
             ||||||||||||||||||||||||||| ||| |
Sbjct:  1382 GATCACCTCTTAACCTTTCAGTTCTGA-GCTGA 1413


>At5g53950 68418.t06229 no apical meristem (NAM) family protein identical to no
           apical meristem protein CUC2 (GI:1944132) [Arabidopsis thaliana];
           contains Pfam PF02365: No apical meristem (NAM) domain;
        Length = 1830

  Plus Strand HSPs:

 Score = 183 (33.5 bits), Expect = 0.51, P = 0.40
 Identities = 147/251 (58%), Positives = 147/251 (58%), Strand = Plus / Plus

Border_2 (35438_LB2D)


TTCATAACCAATCTCGATACACCAAATCGAATTCAAT:TCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAA
TTTGTCCGTATGTACATCTCCCTCCCCTTATCTTGTGTAGTCATCTCTGATGGCTTCTTAGTGTGATGTTTCTCAATAGGTTTGAAAAGCTAGGGAAGTT
ATCATTGTATCTATAGAAATGTGTCTCTCTTTAACTTTGGAATTAGTTTCAAAGATAGTGTGTCCTTGTGATGAATCATATTGTAACAAGATCTTGATTT
ATCCTTTGCTCTCGATACCCTCTCCTCTTGTAGACCTCATAAATCTCTGATCTATTGAGCATTTTACATGATTCAACATTTTACATATTTTTGTAACAAA
TTTTATCATTGGAAAGATTAAATAGCCGTTACATAAGGTAGGTGAATTTTCAATTCAAATGCATCAAAATAGCCGCTATAACCTAAGTGATAACGTTCGG
AAAATGCTTTAGGATAATTCCTTTTTTATTGCTTTTGAAAACGACGACGTTTCTTGTGTCGGACACGTTWTATAACCCMSAMMMACGTNTNTTTTTTCTN
AAAGAAGAGAAGAAGAACTCTCTCT

Start of BLAST Alignment:	NT 1631 / Exon 7 of 7
First 15 bp Perfect Match:	NT > 6 / 3’ UTR

BLAST Details:
Query Sequence: 35438_LB2D 
Length: (624 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At3g20070 68416.t02267 expressed protein                      1382  4.6e-58   1
At4g29470 68417.t03904 phospholipase A2, putative similar...   227  0.037     1
At5g53690 68418.t06194 hypothetical protein                    156  0.43      1
At3g42770 68416.t04131 F-box family protein contains F-bo...   201  0.56      1



>At3g20070 68416.t02267 expressed protein
        Length = 2247

  Plus Strand HSPs:

 Score = 1382 (213.4 bits), Expect = 4.6e-58, P = 4.6e-58
 Identities = 302/325 (92%), Positives = 302/325 (92%), Strand = Plus / Plus

Query:    53 TACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGT-CCGTATG 111
             | | || |||  |||  |   | || |||||| |  | ||  ||  ||  || |||||||
Sbjct:  1928 TTCTTTGAAAGAGTCT-CTC-GAGTCATTAAGCT--C-AATTGTTGATCGGTACCGTATG 1982

Query:   112 TACATCTCCCTCCCCTTATCTTGTGTAGTCATCTCTGATGGCTTCTTAGTGTGATGTTTC 171
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1983 TACATCTCCCTCCCCTTATCTTGTGTAGTCATCTCTGATGGCTTCTTAGTGTGATGTTTC 2042

Query:   172 TCAATAGGTTTGAAAAGCTAGGGAAGTTATCATTGTATCTATAGAAATGTGTCTCTCTTT 231
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2043 TCAATAGGTTTGAAAAGCTAGGGAAGTTATCATTGTATCTATAGAAATGTGTCTCTCTTT 2102

Query:   232 AACTTTGGAATTAGTTTCAAAGATAGTGTGTCCTTGTGATGAATCATATTGTAACAAGAT 291
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2103 AACTTTGGAATTAGTTTCAAAGATAGTGTGTCCTTGTGATGAATCATATTGTAACAAGAT 2162

Query:   292 CTTGATTTATCCTTTGCTCTCGATACCCTCTCCTCTTGTAGACCTCATAAATCTCTGATC 351
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2163 CTTGATTTATCCTTTGCTCTCGATACCCTCTCCTCTTGTAGACCTCATAAATCTCTGATC 2222

Query:   352 TATTGAGCATTTTACATGATTCAAC 376
             |||||||||||||||||||||||||
Sbjct:  2223 TATTGAGCATTTTACATGATTCAAC 2247


>At4g29470 68417.t03904 phospholipase A2, putative similar to secretory low
           molecular weight phospholipase A2 gamma [Arabidopsis thaliana]
           GI:26006457; contains INTERPRO domain IPR001211 phospholipase A2
        Length = 1479

  Plus Strand HSPs:

 Score = 227 (40.1 bits), Expect = 0.038, P = 0.037
 Identities = 139/232 (59%), Positives = 139/232 (59%), Strand = Plus / Plus