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At3g49240 SYNGENTA 39075

Genomic Sequence (from TAIR)
aataaatggataaattatttataaaataacagttataagatagaaaaaaaattactacggatgtggtacaacaaatttctcgttaagagagatatgggcc
ggtcgaagtgccggcctctatagataagccggcaccgcgtatattacgcacaacgtattccgctctttttttgttacatcattgtgcagaatgcagattc
ctaaccaaagaaaaagaaagtttttaaaaatatttaattataattatatgatgaaatcaagaatgggtcctccaaaaatcgagcttatggcccaataaag
cccaaacgtaaatagccaagcccaagcatatttatccacgttcccaaacaatccctaaacctcatcatcatcatctccaaactcctaaaaccctaacaag
aacccttatctctctcatttcctctgaaaggaatcatttttccactcagaaaatctcaaacatttcaaccATGTCGATTTCTAAAGCCGCCTTTTTAAAC
CACCTTCAAACACTATCAAGATCCTACCGCCACCGTGTTCTGCCGCAGCCATTTCTCGCCGTCCGATACATGTCATTCGCGACTCAGGAAGAAGCCGCCG
CTGAACGTCGCCGTCGTAAGCGTCGTCTTCGTATGGAGCCACCGGTTAATTCATTCAACAGATCTCAGCAACAACAATCTCAAATCCCTCGACCAATTCA
AAACCCTAATATCCCGAAATTACCTGAATCTGTCTCTGCTCTCGTTGGGAAACGTCTCGATCTTCATAATCATATCCTGAAATTGATCCGAGAGAATGAT
TTAGAAGAAGCTGCTCTGTACACTCGTCACTCTGTGTATTCGAATTGCCGTCCCACGATTTTCACTGTGAATACTGTTTTAGCGGCGCAGCTTCGACAAG
CTAAGTATGGAGCGCTTCTGCAATTGCATGGATTCATTAATCAAGCTGGTATTGCACCTAATATCATCACATACAATTTGATATTCCAGGCTTATCTTGA
TGTAAGGAAACCTGAAATTGCGTTGGAGCATTACAAGTTGTTTATAGATAATGCTCCATTGAATCCTTCTATTGCTACTTTTAGGATTTTAGTTAAGGGT
TTAGTTAGTAATGACAATCTTGAGAAAGCAATGGAGATTAAAGAGGATATGGCTGTTAAAGGTTTTGTGGTGGATCCTGTTGTTTATAGTTATCTAATGA
TGGGGTGTGTGAAGAATTCTGATGCTGATGGTGTGTTAAAGCTTTATCAGGAGTTAAAGGAGAAGCTTGGTGGGTTTGTTGATGATGGTGTGGTTTATGG
GCAATTGATGAAGGGGTATTTCATGAAGGAGATGGAGAAAGAGGCTATGGAGTGTTATGAGGAAGCTGTTGGAGAGAATTCAAAGGTTAGGATGAGTGCT
ATGGCTTATAATTATGTGCTGGAGGCTTTGAGTGAGAATGGGAAGTTTGATGAGGCTTTGAAGTTGTTTGATGCGGTGAAAAAGGAACATAATCCACCGA
GGCATTTGGCTGTAAATTTGGGAACTTTTAATGTGATGGTTAATGGGTACTGTGCTGGAGGAAAGTTTGAGGAAGCTATGGAGGTTTTTAGGCAAATGGG
TGATTTTAAGTGTAGTCCTGATACTTTATCGTTCAACAATCTGATGAATCAGCTGTGTGACAACGAGTTGTTGGCTGAGGCTGAGAAGCTTTATGGTGAA
ATGGAAGAGAAAAATGTGAAGCCTGATGAGTACACATATGGTTTGTTAATGGATACTTGTTTCAAGGAAGGTAAGATAGATGAAGGAGCTGCGTATTACA
AAACAATGGTTGAGTCTAATCTAAGACCAAATTTGGCTGTGTATAACCGTTTGCAAGATCAGTTGATCAAAGCTGGGAAACTCGACGATGCAAAATCTTT
CTTCGATATGATGGTGAGCAAACTCAAGATGGACGATGAGGCTTACAAATTCATCATGAGGGCGTTAAGTGAAGCTGGTAGACTCGACGAGATGCTCAAG
ATTGTGGATGAGATGCTGGATGATGACACGGTAAGAGTGAGTGAGGAACTGCAAGAGTTTGTGAAAGAAGAACTGAGGAAAGGAGGAAGAGAGGGAGATC
TGGAGAAACTAATGGAAGAGAAAGAGAGATTGAAAGCAGAGGCAAAGGCGAAAGAGTTAGCAGATGCAGAAGAGAAGAAGAAGGCTCAAAGTATTAACAT
AGCTGCATTAATTCCTCCAAAAGCAGTGGAAGAAAAGAAAGAAACCGCAAAACTGCTTTGGGAAAACGAAGCTGGTGGTGTTGAAGAAGCCGATGTGGTG
GAGATGGCTAAAGGAGTAGAAGCAGGTGGATCCAATGGTCAGGATCCACCGTCCTGCTGAtcttatttttttccttgaattcattagactctagttatct
tcttatcattcaacttttatgtaattagggatttgtttgggacaataagctatgattaatcttttgctataatgcaggtgtttgcaaaatcttttttcc
atgtttgcttttgatttcaaattaacatgctatgtagaacatttgttttagtggatggtctatgtaatcatttctttattactcaatttggtgcattata
tacgtaacaagaaattgtaaatcttatccatttgggacaaagatttgaagtgaggaaagactgtattccaactacatgaagcaatagaaactgagcat
ctgattagaaagcctagaagattgattatgtacacagcaaactggtcaagtcaaaagcaaaacagacagagattggaaacagcataaccaaaactaaccg
atctactacccttcaaggaaatttattaaaatctagtatatctgataagatgcattccatggaagcaatctatcaaaaacttagctggaaacaagttctt
atagtcaaaataggcttcctacttatagtcaaaataggcttcctatagaagcaagggtcactgtaactccgagctctatgcttctttgcttgcttttttt
TCATCTGGGTGTGTTCATTGGctggggcaccaccaacacataaacagaccaatgttagccatataaaactttcagatagaggtatttgaagctttgaatt
aaaactgaaacaaagatatgatcatatagacaatttcatgatatgcttcaacagacatatactatgatagaagagaagggtcagaagatacatgaaaact
agttttaatacCTGATTAGTGCATGAACTCTGAGCCAAGCATCGAACAAATTCTTCTTTCTTCTTGCCAAAGTTGAAGACGGCATGCTCACGGAGCACTT
GCTCCCGCTTAATGTCCTCCAGCGTTTTCTCCTTTTTCCATTTCAGCTCTTTCATTTTCTTCTCCGCCTCACTATAGTTGTCCATCGTGCGAGAAGCAGT
TGGCTTTGCAAATCTCACGTTCACCTCATCActgaaaatcatattgatttcagttcaggacagcggataagagcaagaaggaaatggaagaatccaaaga
ggg

Border_1 (39075_LB1_Contig2_U)

ATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTACACCACAATATATCAGTATTAACATAGC
TGCATTAATTCCTCCAAAAGCAGTGGAAGAAAAGAAAGAAACCGCAAAACTGCTTTGGGAAAACGAAGCTGGTGGTGTTGAAGAAGCCGATGTGGTGGAG
ATGGCTAAAGGAGTAGAAGCAGGTGGATCCAATGGTCAGGATCCACCGTCCTGCTGATCTTATTTTTTTCCTTGAATTCATTAGACTCTAGTTATCTTCT
TATCATTCAACTTTTATGTAATTAGGGATTTGTTTGGGACAATAAGCTATGATTAATCTTTTGCTATAATGCAGGTGTTTGCAAAATCTTTTTTCCATGT
TTGCTTTTGATTTCAAATTAACATGCTATGTAGAACATTTGTTTTAGTGGATGGTCTATGTAATCATTTCTTTATTACTCAATTTGGTGCATTATATACG
TAACAAGAAATTGTAAATCTTATCCATTTGGGACAAAGATTTGAAGTGAGGAAAGACTGTATTCCAACTACATGAAGCAATAGAAACTGAGCATCTGATT
AGAAAGCCTAGAAGATTGATTATGTACACAGCAAACTGGTCAAGTCAAAAGCAAAACAGACAGAGATTGGAAACAGCATAACCAAAACTAACCGATCTAC
TACCCTTCAAGGAAATTTATTAAAATCTAGTATATCTGATAAGATGCATTCCAT

Start of BLAST Alignment:NT > 125 / 3’ UTR
First 15 bp Perfect Match:NT > 125 / 3’ UTR
BLAST Details:
Query Sequence: 39075_LB1_Contig2_U reverse complement 
Length: (754 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At3g49240 68416.t04998 pentatricopeptide (PPR) repeat-con...  1480  1.8e-62   1
At3g49250 68416.t04999 expressed protein                      1060  1.8e-42   1
At5g55250 68418.t06392 S-adenosyl-L-methionine:carboxyl m...   236  0.027     1
At1g65960 68414.t06861 glutamate decarboxylase 2 (GAD 2) ...   206  0.53      1



>At3g49240 68416.t04998 pentatricopeptide (PPR) repeat-containing protein
           contains Pfam profile PF01535: PPR repeat
        Length = 2122

  Minus Strand HSPs:

 Score = 1480 (228.1 bits), Expect = 1.8e-62, P = 1.8e-62
 Identities = 296/296 (100%), Positives = 296/296 (100%), Strand = Minus / Plus

Query:   668 AGTATTAACATAGCTGCATTAATTCCTCCAAAAGCAGTGGAAGAAAAGAAAGAAACCGCA 609
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1827 AGTATTAACATAGCTGCATTAATTCCTCCAAAAGCAGTGGAAGAAAAGAAAGAAACCGCA 1886

Query:   608 AAACTGCTTTGGGAAAACGAAGCTGGTGGTGTTGAAGAAGCCGATGTGGTGGAGATGGCT 549
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1887 AAACTGCTTTGGGAAAACGAAGCTGGTGGTGTTGAAGAAGCCGATGTGGTGGAGATGGCT 1946

Query:   548 AAAGGAGTAGAAGCAGGTGGATCCAATGGTCAGGATCCACCGTCCTGCTGATCTTATTTT 489
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1947 AAAGGAGTAGAAGCAGGTGGATCCAATGGTCAGGATCCACCGTCCTGCTGATCTTATTTT 2006

Query:   488 TTTCCTTGAATTCATTAGACTCTAGTTATCTTCTTATCATTCAACTTTTATGTAATTAGG 429
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2007 TTTCCTTGAATTCATTAGACTCTAGTTATCTTCTTATCATTCAACTTTTATGTAATTAGG 2066

Query:   428 GATTTGTTTGGGACAATAAGCTATGATTAATCTTTTGCTATAATGCAGGTGTTTGC 373
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2067 GATTTGTTTGGGACAATAAGCTATGATTAATCTTTTGCTATAATGCAGGTGTTTGC 2122


>At5g55250 68418.t06392 S-adenosyl-L-methionine:carboxyl methyltransferase
           family protein similar to SAM:jasmonic acid carboxyl
           methyltransferase (JMT)[GI:13676829] and to SAM:benzoic acid
           carboxyl methyltransferase (BAMT)[GI:9789277][Antirrhinum majus]
        Length = 3396

  Minus Strand HSPs:

 Score = 236 (41.5 bits), Expect = 0.027, P = 0.027
 Identities = 192/319 (60%), Positives = 192/319 (60%), Strand = Minus / Plus


Border_2 (39075_AbB_U_aux39075_3_jm33_ab1)

AATCAATTNATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTCACANCTNATTCATGANGTGC
TTAGCAGAACGTGGATCCATCCCTGGCANNANCACCNTNANAGAGGANNGNAANCGAGCAACTGCTTGACAGAAANCTNAGGGCCTTNNCAGTCNTGANA
NCCGGACGCTGTNTNTAAGNCNTATGGNGGNTAAGCAGNCGGATCCAANGTTNCGGATNCNCCGANCTNCTGNNCTTATCTNCNTANTNGAAAACAGTAC
ACNCGAAATGACACCCNCNCTTGAANCTGNNCACGTANGTNGGGAAGATGGTTGATGACATCACATCTATGCATTATTCACNNTGATATANGCGCAGGCT
GNTGGGCAAAGGTCTTTGNTAGGCATGGTCCACTCCGCNATNGTCNNNNTAANNNNNTTNNNCTNTANCATNATGGCTNNAGGGATGGGCTATNTNTACT
ATTTACTTTCATNNCNNANGGCTATAGCNCTACTATANAGCACANCGANATAAGGAANTCTNTCCCCCNCNCCGGGNACAAAAGATTTTGAAAGTGAGGA
AAGACTGTATTCCAACCTACATGAAGCAATAGAAACTGAGCATCTGATTAGAAAGCCTAGAAGATTGATTATGTACACAGCAAACTGGTCAAGTCAAAAG
CAAAACAGACAGAGATTGGAAACAGCATAACCAAAACTAACCGATCTACTACCCTTCAAGGAAATTTATTAAAATCTAGTATATCTGATAAGATGCATT


Start of BLAST Alignment:NT > 272 / Downstream
First 15 bp Perfect Match:NT > 288 / Downstream; 3’ UTR of At3g49250
BLAST Details:
Query Sequence: 39075_AbB_U_aux39075_3_jm33_ab1 
Length: (799 letters) 
Database: TIGR AGI BAC Sequences (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

F2K15 67266                                                   1058  1.3e-41   1
F5H8 67722                                                     509  8.3e-17   1
F24C7 67720                                                    509  8.3e-17   1
K7M2 60539                                                     500  2.1e-16   1


F2K15 67266
        Length = 129,757

  Plus Strand HSPs:

 Score = 1058 (164.8 bits), Expect = 1.3e-41, P = 1.3e-41
 Identities = 220/225 (97%), Positives = 220/225 (97%), Strand = Plus / Plus

Query:   575 GGNACAAAAGATTTTGAAAGTGAGGAAAGACTGTATTCCAACCTACATGAAGCAATAGAA 634
             || ||||| ||||| ||| ||||||||||||||||||||||| |||||||||||||||||
Sbjct: 60905 GGGACAAA-GATTT-GAA-GTGAGGAAAGACTGTATTCCAAC-TACATGAAGCAATAGAA 60960

Query:   635 ACTGAGCATCTGATTAGAAAGCCTAGAAGATTGATTATGTACACAGCAAACTGGTCAAGT 694
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 60961 ACTGAGCATCTGATTAGAAAGCCTAGAAGATTGATTATGTACACAGCAAACTGGTCAAGT 61020

Query:   695 CAAAAGCAAAACAGACAGAGATTGGAAACAGCATAACCAAAACTAACCGATCTACTACCC 754
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61021 CAAAAGCAAAACAGACAGAGATTGGAAACAGCATAACCAAAACTAACCGATCTACTACCC 61080

Query:   755 TTCAAGGAAATTTATTAAAATCTAGTATATCTGATAAGATGCATT 799
             |||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61081 TTCAAGGAAATTTATTAAAATCTAGTATATCTGATAAGATGCATT 61125


F5H8 67722
        Length = 80,098

  Minus Strand HSPs:

 Score = 509 (82.4 bits), Expect = 8.3e-17, P = 8.3e-17
 Identities = 201/325 (61%), Positives = 201/325 (61%), Strand = Minus / Plus