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At1g55900 SYNGENTA 42230

Genomic Sequence (from TAIR)
atgtagtcgaggtttaggcagaagaaaccctacaaatttaataaattttggttatttgttttctctttttgacacgaatgaatttattatttttgttgga
aaaatattttcattttatttggtaaataaatagtgtaaaataacttcattaattagttattaaaagcccgaaatgcaaaaataaaataaaatagattctc
ttcttggtaaacaagtaaaaaaaacagcaaaaaaaatagaaaaatatcggagtagaaataaaccgactaccaaacagtggataagagagttaaaacgagc
ccataaaacctgtttccaaacagtccatctgttaattttccttttctccgatcaattccaaaaccctacttcaaccctgcgattcattcatcattcacag
agtttgaagaaaacgccgaaatcggaaATGGCGTCTATTGTTCTTCGATCTCGCCTGTTGCCAAGATTAGCAAAGCTCAGATCTCGTAATCTGAGATGTT
TCTCTGCGGAAGCATCTTCGACGAATTCTACATCTAGGTATTCGGGAGTCACCTCTACCCAGTCGATGTTTTCTGATTTTCCACCGCCAAATCAGCCGCC
ACCTCCTCCTCCTCCTCAAGTGGAAGCTGCTGCCGCGGCTGCGACGGGAAAGGAGAGGAAAGGTCTTAAGTATCTTGGTTACGCACTCCTCTGGGCACTC
ACTGGTGCTACCGCTGCTACTGGTTACGCAAGTTTTGgtatgtacttgcattcatgaattatcttttttccctctcgtagatttctagtacaaagttgtt
tttgctgtgaaatgcgagtaaaaatgtgttatggtttgcttaatcgtggcattgcattacattttcacatatcagtcttttgaattcagtgttcttcact
tggtagaactgtgtctttcttcgttatgttgactctttttagttctgattatacttgtttgtttcgttatgcagCTTACACGATAGATGAAGTGAATGAA
AAGACTAAGGCGTTCCGAGAATCAGCTACGAAGACACCAGTTATTAAATCTTCTGGCATTGATGTGATTGATgtgagtaagcttggtagccttgagtcgc
tgtcaaattaatgttgatcataacttgcttctgtattagttgactttctttagtctctggctttcaaatgcgtgctgccatgtttctctgcttcaaggta
ttgtttataagcttctctgaattaataccctttatactcatctttctgcagAAATATCAGACTAAGTTGTACTCTGCTGCAATGACAGgtatagttcttg
cgcctctgtcatcataactaatagtatgtctttaattaagcatagcatgagttggtttgcttgaattttgatcagGTTCTGCTAGAGCCATTGATAAGTA
TTTAGAGCTTAGGGAAATTGTGGAGGAACAAGTGAAAgtacgtcctttcaccgagacacaacttttctttttcttgcatcttcttttttcttctagaatt
cttattttttgtctggttccttatagGGCTTCACGGAACCTCTCTCGGAAAAGCTTCTTCCGGATCTGCACCCCGCAGAACAGCATGTCTTCACTCTAGT
TCTTGATTTAAATGAGACACTGCTTTACACTGACTGGAAGgtaacatttcatttatttactgatcatcaaaatacttgcatcatagatgtgaatatgtga
ctcagagtatctatgttatgaaacaaagcagttctggtgaactactttgttttcttaagtagtgctgtatttaacttttcctgccttttcatttacaaaa
atgtctagCGGGAAAGAGGGTGGAGGACATTCAAAAGACCAGGTGTCGATGCATTCTTGGAGCATCTTGGGAAATTCTATGAGATTGTCGTGTATTCTGA
CCAGATGGAGATGgtaaagtcccaattggtttttcttttttctccttttttgcttgtaatatcatagtaatgcgactactaacgcttgtttcattacttg
tagTATGTACTCCCGGTCTGTGAGAAATTAGACCCAAATGGCTACATACGTTATAAGCTAGCAAGAGGCGCTACTAAATATGAAAATGGAAAGCACTATA
GGgtacgtaagcagctagcttatcttgtcaaagcatactttctgacttgcccgagtaactgtttctcaactaactattaaaattcctgctgcagGATCTG
TCAAAGCTGAACAGAGATCCGAAGAAGATTCTGTTTGTTAGCGCAAATGCCTTTGAATCAACCCTTCAGCCAGAAAATAGCGTGCCAATCAAGCCTTACA
AACTAGAAGCTGATGACACAGCCCTAGTTGACCTGATACCCTTCCTTGAATgtaagtattggtgaactgagggataagttctgttccagtccgtgaactg
cctgtatcaattttgtttggttgatcttcctgtttgggttatgcagATGTTGCACGTAACAGTCCAGCAGACATCAGACCGGTTCTGGCCTCTTTTGAGA
GAAAAGATATTGCCAAAGAGTTCATTGACAGGTCCATAGAGTATCAAAAgtgagtcgatcagagctttttttttaccctatcgtatcaatgcaaaatc
tgttggtagtgtcccactaagagtgtcatatctctatgcagGCGAAAGCAAGGACAACTGGGACAAGGTCGATTCTGGAGGCGTTAAaggatctaagatt
tatgtatagagcgagagacaattatatcatcattgatggtgcacaccataaaataggaacatgagctaccgttcttcgctatggatttattaggtaaaa
tccgaattaaaatactgtctttgtcatcaaagagtttttatgttacccaattttcctcgtctttctctctgctttacatagaaaatttctatgagaacga
aaaagaaattatcatatggaaggttcatttttcgttgactaacaaataccgttttttttctctgatttatttttacttggtttgacaaatgatttatgat
tatgaactttgagaaattattggttgaaacgtcaatttgtatgtattgctaaaaaaaagagtaaattaccttttctagtacgcttaatatttatctgtct
gtt

Border_1 (42230_LB1_D)

AATCGAATTCAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTACACCACAATATATCCTG
TCATAAAATAGGAACATGACAGGAACATATCATGTTCCTAATAAATCCACAATATTCGACTCACTTTTGATACTCTATGGACCTGTCAATGAACTCTTTG
GCAATATCTTTTCTCTCAAAAGAGGCCAGAACCGGTCTGATGTCTGCTGGACTGTTACGTGCAACATCTGCATAACCCAAACAGGAAGATCAACCAAACA
AAATTGATACAGGCAGTTCACGGACTGGAACAGAACTTATCCCTCAGTTCACCAATACTTACATTCAAGGAAGGGTATCAGGTCAACTAGGGCTGTGTCA
TCAGCTTCTAGTTTGTAAGGCTTGATTGGCACGCTATTTTCTGGCTGAAGGGTTGATTCAAAGGCATTTGCGCTAACAAACAGAATCTTCTTCGGATCTC
TGTTCAGCTTT

Start of BLAST Alignment:NT 2148 / Intron 9 of 9
First 15 bp Perfect Match:NT 2131 / Intron 9 of 9
BLAST Details:
Query Sequence: 42230_LB1_D 
Length: (511 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At1g55900 68414.t05868 NLI interacting factor (NIF) famil...  1784  2.7e-76   1



>At1g55900 68414.t05868 NLI interacting factor (NIF) family protein contains
           Pfam profile PF03031: NLI interacting factor
        Length = 2661

  Minus Strand HSPs:

 Score = 1784 (273.7 bits), Expect = 2.7e-76, P = 2.7e-76
 Identities = 364/373 (97%), Positives = 364/373 (97%), Strand = Minus / Plus

Query:   511 AAAGCTGAACAGAGATCCGAAGAAGATTCTGTTTGTTAGCGCAAATGCCTTTGAATCAAC 452
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1871 AAAGCTGAACAGAGATCCGAAGAAGATTCTGTTTGTTAGCGCAAATGCCTTTGAATCAAC 1930

Query:   451 CCTTCAGCCAGAAAATAGCGTGCCAATCAAGCCTTACAAACTAGAAGCTGATGACACAGC 392
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1931 CCTTCAGCCAGAAAATAGCGTGCCAATCAAGCCTTACAAACTAGAAGCTGATGACACAGC 1990

Query:   391 CCTAGTTGACCTGATACCCTTCCTTGAATGTAAGTATTGGTGAACTGAGGGATAAGTTCT 332
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1991 CCTAGTTGACCTGATACCCTTCCTTGAATGTAAGTATTGGTGAACTGAGGGATAAGTTCT 2050

Query:   331 GTTCCAGTCCGTGAACTGCCTGTATCAATTTTGTTTGGTTGATCTTCCTGTTTGGGTTAT 272
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2051 GTTCCAGTCCGTGAACTGCCTGTATCAATTTTGTTTGGTTGATCTTCCTGTTTGGGTTAT 2110

Query:   271 GCAGATGTTGCACGTAACAGTCCAGCAGACATCAGACCGGTTCTGGCCTCTTTTGAGAGA 212
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2111 GCAGATGTTGCACGTAACAGTCCAGCAGACATCAGACCGGTTCTGGCCTCTTTTGAGAGA 2170

Query:   211 AAAGATATTGCCAAAGAGTTCATTGACAGGTCCATAGAGTATCAAAAGTGAGTCGAATAT 152
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||  | 
Sbjct:  2171 AAAGATATTGCCAAAGAGTTCATTGACAGGTCCATAGAGTATCAAAAGTGAGTCGATCAG 2230

Query:   151 TGTGGATTTATTA 139
              |    ||| |||
Sbjct:  2231 AGCTTTTTTTTTA 2243


Border_2 (42230_AbB_U)

AGTGATCAGATCCGCACACCATAAAATAGGAACATGAGCTACCGTTCTTCGCTATGGATTTATTAGGTAAAATCCGAATTAAAATACTGTCTTTGTCATC
AAAGAGTTTTTATGTTACCCAATTTTCCTCGTCTTTCTCTCTGCTTTACATAGAAAATTTCTATGAGAACGAAAAAGAAATTATCATATGGAAGGTTCAT
TTTTCGTTGACTAACAAATACCGTTTTTTTTCTCTGATTTATTTTTACTTGGTTTGACAAATGATTTATGATTATGAACTTTGAGAAATTATTGGTTGAA
ACGTCAATTTGTATGTATTGCTAAAAAAAAGAGTAAATTACCTTTTCTAGTACGCTTAATATTTATCTGTCTGTTTCAATGCGTGTCTTAAGGTGCAATA
TCAGGTTTACAATTAGGTAAATCAATTACAGATTTTTCTTGTTAATCTGAACCAAATAATTGTACTTTTTCCTAAATTTGAATTCATAAA

Start of BLAST Alignment:NT > 53 / 3’ UTR
First 15 bp Perfect Match:NT > 53 / 3’ UTR
BLAST Details:
Query Sequence: 42230_AbB_U 
Length: (490 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At1g55900 68414.t05868 NLI interacting factor (NIF) famil...  1219  7.3e-50   1
At1g79420 68414.t08464 expressed protein contains Pfam pr...   224  0.022     1
At2g16385 68415.t01780 expressed protein Requires functio...   191  0.59      1
At2g16990 68415.t01858 expressed protein                       189  0.80      1


>At1g55900 68414.t05868 NLI interacting factor (NIF) family protein contains
           Pfam profile PF03031: NLI interacting factor
        Length = 2661

  Plus Strand HSPs:

 Score = 1219 (188.9 bits), Expect = 7.3e-50, P = 7.3e-50
 Identities = 247/255 (96%), Positives = 247/255 (96%), Strand = Plus / Plus

Query:    14 GCACACCATAAAATAGGAACATGAGCTACCGTTCTTCGCTATGGATTTATTAGGTAAAAT 73
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2407 GCACACCATAAAATAGGAACATGAGCTACCGTTCTTCGCTATGGATTTATTAGGTAAAAT 2466

Query:    74 CCGAATTAAAATACTGTCTTTGTCATCAAAGAGTTTTTATGTTACCCAATTTTCCTCGTC 133
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2467 CCGAATTAAAATACTGTCTTTGTCATCAAAGAGTTTTTATGTTACCCAATTTTCCTCGTC 2526

Query:   134 TTTCTCTCTGCTTTACATAGAAAATTTCTATGAGAACGAAAAAGAAATTATCATATGGAA 193
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  2527 TTTCTCTCTGCTTTACATAGAAAATTTCTATGAGAACGAAAAAGAAATTATCATATGGAA 2586

Query:   194 GGTTCATTTTTCGTTGACTAACAAATACCGNNNNNNNNCTCTGATTTATTTTTACTTGGT 253
             ||||||||||||||||||||||||||||||        ||||||||||||||||||||||
Sbjct:  2587 GGTTCATTTTTCGTTGACTAACAAATACCGTTTTTTTTCTCTGATTTATTTTTACTTGGT 2646

Query:   254 TTGACAAATGATTTA 268
             |||||||||||||||
Sbjct:  2647 TTGACAAATGATTTA 2661


>At1g79420 68414.t08464 expressed protein contains Pfam profile PF04788:
           Protein of unknown function (DUF620)
        Length = 2858

  Plus Strand HSPs:

 Score = 224 (39.7 bits), Expect = 0.023, P = 0.022
 Identities = 212/378 (56%), Positives = 212/378 (56%), Strand = Plus / Plus