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At5g08170 SYNGENTA 43301

Genomic Sequence (from TAIR)
gcatagctatatatctttttgaattgttatgttagaagtttgttgaaatgaaagattacggttgaaatgacaataaaaacaagaaaagaaaaagaaaaat
atcaaaacaacttttgtcgtgtttgggtcactagattgggtttaggattccgattctgctcgcctctggattctgattccgctcaccactaatagtgggg
tcgtgcccggtcgtgtaatgagcataagtttcagtcgaatagttaaaaagatggggtccaactgtaagaaaatatattctatacgggtctcttaagaaaa
ctgagaaattgtttcggaatctttttgtgtacctttctttttttttctcttcttggtcagaaaaagaagtattaggaatcaataacgcatacggtataca
catcagtttaaagcttacatctgacttctctctcacaaaaacatcgtgaagaATGGAGGAGTCACGAGAATCGCCGGCGGAACACGGCTACTACATGCCG
GCGGAATGGGATTCTCATGCTCAAACTTGGATCGGTTGGCCTgtaattctctatctctcttcctcttttctttttttttcatgttttattcattaacct
gagataaagtaaaaattgacggcgattctggagaactcaatctcgctgttcaaaaatcaaacttcgatttcgattttgtcctttttttttttttatat
ggatcctgctcgtagtttatcattgttcgaattgctgttttctgtttatttgatgatttctctgcttcctggttcgtttttgtagagtttccgataaat
tttgtatagcttctgtatgtcaagttagcatctagatgtgacatgagatgagatcttttacattgcaatctatcatggttacagattctgcaaccaaatc
agaatttaaagcattttcagttaggttgtttgtttcgatttgtttcatcactgaatacatgaatcaaacctcaaaccacttttgtgattttgaaatctgg
attataattctgtcataatatggctgcagGAACGGCAAGATAACTGGCGGCACAACGCTTTACCCGCACAACGAGTGTTTGCAGATGTTGCAAAGGCCAT
CTCAAAGTTCGAGCCTGTCACTGTCTGTGCTAGCCCTGCTCAGgtttgtttacaatcatacctgaaataagaaaacatctttgagtttctacatacagat
accaagtagtctctcagtttactttctgtttttggcttacatgattgtgcttgcattagTGGGAAAATGCAAGGAAACAGCTTCCAGAGGATATAAGAGT
TGTTGAGATGAGCATGAATGATTCTTGGTTCCGCGACTCTGGACCAACTgtgagtttacttctttagcttctcccttttctcaccatcttacaaagcttt
ctaacaatttgttgttaccgctctcttttatacagTTCATTGTACGGAAAAGACCAGTAAAGCTCAGTTCTCTTAACCGAAACATTGCTGGGATCGACTG
GAATTTCAATGCCTGGGGAGgtaaggatttggaaacctttttacattctcaactatttgtagtcactgcttgactcacttttcccatggcagGAGCTAAT
GATGGCTGTTACAATGATTGGAGTCATGACCTTTTAGTTTCACGAAAGgtaccgtatttggagaaataaaccttctagtcattattggtttccatatcac
taacattagcatattacaaatattgatttgctctttcttctcctacccatcagATTCTCGCTTTGGAACGAATTCCAAGATTTCAACATTCGATGATTCT
TGAAGGAGGCAGCATCCATGTCGATGGGGAAGgtttgcatacatttcatagtgacttgaaaacgcaataacttgcaaaaaaacagattatctgagtttat
aattgttcacaatcccactttgttgctttgcttattctactttcagGAACCTGCCTTGTCACAGAAGAGTGTCTCTTGAACAAAAACCGAAACCCTCATA
TGAGTAAAGAGCAAATAGAGGAAGAACTTAAGAAGTACCTCGGAGTACAATCATTTATCTGGCTTCCTCGTGGTCTTTACGgtactccacttgctacatc
ggttacaattgccatcgcttatttcgaaagaaataattgtcaatcttatagaaattttaatggttgtcagGGGATGAGGACACAAATGGTCACATTGATA
ACATGTGCTGCTTCGCTAGACCGGGAGTTGTGTTATTGTCTTGGACAGACGATGAAACCGATCCTCAATACGAAAGGTCTGTGGAAGCTCTTTCGGTTTT
GTCGAATTCGATTGATGCTCGTGGAAGGAAGATTCAAGTCATTAAACTTTATATCCCGGAACCCCTTTATATGACTGAAGAAGAATCGTCTGGAATCACT
CAGgtaatgaatactgactagataaaactcaatcttttgaagttgttttgaaacaaatatttcatcgatttcattgttgcagGATGGTGAAGCTATACCA
AGACTTGCAGGGACAAGACTCGCAGCATCGTATGTGAATTTCTACATCGCCAATGGAGGAATAATCGCTCCACAATTCGGTGATCCAATACGTGATAAAG
AAGCGATTCGTGTCCTCTCGGATACATTTCCTCATCACTCGgtattaaaaccatctctgtttcatttcatggatcaaaaacaaaaacagattttgatttt
tcttcttcttcttctgaattttttttgaagGTTGTGGGAATCGAGAATGCAAGAGAGATCGTTCTTGCTGGAGGAAACATTCATTGTATAACGCAGCAGC
AGCCGGCGGAGCCTACTTCCGTCGCCGAAAATGGCCACTGActgtctctgcgcgccaccgagggatttatgaatcctggccgttgatattttaaaataca
gatttgaagcttgaggacgaacgagagccgttcattaattggatcctgtattttatggttcgttgactaagaaatatattattgggtcatcattccgatc
aaactttgcttgcattatggcataacaattagatttttgttctgttaattatgcaagtttgaataattaatttacatttgtatttttttagtagaatttt
cattttttttttcttaatttggccattcaaatctctacttttgaagtatttcattgttagttgttatattagttccatattacatgatgactactaaatt
gtggattataaactgattgtcttttgttggtacgactttttctcgttaatgattttgttggatatattagcgttatgtttatttacccgaaccaattagc
taaa

Border_1 (43301_LBU)

GCCTTTTCAGAAATGGATAAATAGCCTTGCTTCCTATTATATCTTCCCAAATTACCAATACATTACACTAGCATCTGAATTTCATAACCAATCTCGATAC
ACCAAATCGAATTCAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTAATGAATAAAACAT
GAAAAAAAAAGAAAAGAGGAAGAGAGATAGAGAATTACAGGCCAACCGATCCAAGTTTGAGCATGAGAATCCCATTCCGCCGGCATGTAGTAGCCGTGTT
CCGCCGGCGATTCTCGTGACTCCTCCATTCTTCACGATGTTTTTGTGAGAGAGAAGTCAGATGTAAGCTTTAAACTGATGTGTATACCGTATGCGTTATT
GATTCCTAATACTTCTTTTTCTGACCA:AGAAGAGAAAAAAAAAGAAGGGTCCCCAAAANGATTCCGAAACAATTTNTCAGTTTTTTTAAAANACCCGTA
TANAATATATTTTNTTACAGTTGGNCCCCATTTTTTTAACTATTCGACTGAAACTTATGCTCATTACACGACCGGGCACNACCCCACTNTTAGGGGGGAG
CGGAATCANAATCCAGAGGCNAGCANAATCGGAATCCTAAACCCAATNTAGNGACCCAAACACNACAAAAGTGGGTTGGAAATTTTTCTTTTTCTTTTCT
NGGTTTTATGGCNATTTCAACCGTAATCTTTCATTTCAACAAACTTTTAACATAACAATTCAAAAAGATATATAGCTATGCCAAAWAAAA:GTTGGGTAT
AA:CTTAATGTTGATTATTAACAAAAACTAAATTAGTCTGTATAATAAATAATGATG

Start of BLAST Alignment:NT 279 / Intron 1 of 9
First 15 bp Perfect Match:NT 144 / Intron 1 of 9
BLAST Details:
Query Sequence: 43301_LBU 
Length: (854 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At5g08170 68418.t00873 porphyromonas-type peptidyl-argini...   832  5.2e-32   1



>At5g08170 68418.t00873 porphyromonas-type peptidyl-arginine deiminase family
           protein contains Pfam PF04371: Porphyromonas-type peptidyl-arginine
           deiminase
        Length = 2759

  Minus Strand HSPs:

 Score = 832 (130.9 bits), Expect = 5.2e-32, P = 5.2e-32
 Identities = 244/324 (75%), Positives = 244/324 (75%), Strand = Minus / Plus

Query:   364 ACATCTGACTTCTCTCTCACAAAAACATCGTGAAGAATGGAGGAGTCACGAGAATCGCCG 305
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:     1 ACATCTGACTTCTCTCTCACAAAAACATCGTGAAGAATGGAGGAGTCACGAGAATCGCCG 60

Query:   304 GCGGAACACGGCTACTACATGCCGGCGGAATGGGATTCTCATGCTCAAACTTGGATCGGT 245
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:    61 GCGGAACACGGCTACTACATGCCGGCGGAATGGGATTCTCATGCTCAAACTTGGATCGGT 120

Query:   244 TGGCCTGTAATTCTCTATCTCTCTTCCTCNNNNNNNNNNNNNNCATGTTTTATTCATTAA 185
             |||||||||||||||||||||||||||||              |||||||||||||||||
Sbjct:   121 TGGCCTGTAATTCTCTATCTCTCTTCCTCTTTTCTTTTTTTTTCATGTTTTATTCATTAA 180

Query:   184 ACAAAT-TGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTA-CTGAAT 127
              |  |  | | |  || | || || |   |  |||  ||| |   | ||| |  |||   
Sbjct:   181 CCTGAGATAAAG--TAAA-AA-TTGACGGCG-ATTCTGGA-GAACTCAATCTCGCTGTTC 234

Query:   126 TAACGCCGAA-TT-GAATTCGATTTGGTG-TATCGAGATTGGT-TATGAAATTCAGATGC 71
              ||   | || || || |||||||| ||  | |     ||  | |||| | | | | |  
Sbjct:   235 AAAAATCAAACTTCGATTTCGATTTTGTCCTTTTTTTTTTTTTATATGGA-TCCTGCTCG 293

Query:    70 TAGTGTAATGTATTGGTA-ATTTG 48
             |||| || |  |||| |  | |||
Sbjct:   294 TAGTTTA-TC-ATTGTTCGAATTG 315


Border_2 (43301_RBD)

ATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGA
:TACGCCAAGCTCGAAATTAACCCTCACTAAAGGGAACAAAAGCTGGAGCTCGCTTGAGCTTGGATCAGATTGTCGTTTCCCGCCTTCAGTTTAAACTAT
CAGTGTTTCTGGCGATTCTGGAGAACTCAATCTCGCTGTTCAAAAATCAAACTTCGATTTCGATTTTGTCCTTTTTTTTTTT

Start of BLAST Alignment:NT 151 / Intron 1 of 9
First 15 bp Perfect Match:NT 167 / Intron 1 of 9
BLAST Details:
Query Sequence: 43301_RBD 
Length: (281 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At5g08170 68418.t00873 porphyromonas-type peptidyl-argini...   311  3.0e-08   1
At3g48425 68416.t04908 endonuclease/exonuclease/phosphata...   151  0.99      1



>At5g08170 68418.t00873 porphyromonas-type peptidyl-arginine deiminase family
           protein contains Pfam PF04371: Porphyromonas-type peptidyl-arginine
           deiminase
        Length = 2759

  Plus Strand HSPs:

 Score = 311 (52.7 bits), Expect = 3.0e-08, P = 3.0e-08
 Identities = 71/79 (89%), Positives = 71/79 (89%), Strand = Plus / Plus

Query:   192 TAAACTATCAGTGTTTCTGGCGATTCTGGAGAACTCAATCTCGCTGTTCAAAAATCAAAC 251
             |||| ||  | | |  | ||||||||||||||||||||||||||||||||||||||||||
Sbjct:   188 TAAAGTAAAAAT-TGAC-GGCGATTCTGGAGAACTCAATCTCGCTGTTCAAAAATCAAAC 245

Query:   252 TTCGATTTCGATTTTGTCC 270
             |||||||||||||||||||
Sbjct:   246 TTCGATTTCGATTTTGTCC 264


>At3g48425 68416.t04908 endonuclease/exonuclease/phosphatase family protein
           similar to SP|P51173|APEA_DICDI DNA-(apurinic or apyrimidinic site)
           lyase (EC 4.2.99.18)(Class II
           apurinic/apyrimidinic(AP)-endonuclease) {Dictyostelium discoideum};
           contains Pfam profile PF03372: Endonuclease/Exonuclease/phosphatase
           family
        Length = 2927

  Minus Strand HSPs:

 Score = 151 (28.7 bits), Expect = 4.5, P = 0.99
 Identities = 53/74 (71%), Positives = 53/74 (71%), Strand = Minus / Plus