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At3g20440 SYNGENTA 48882

Genomic Sequence (from TAIR)
aaatctgtaaaaatatgtgaatgttaaaaatttatcaaaagaaaaccatactcgtgttgaatttaattaaaagtaaataaaaaacgagaaatctcacttt
caagatcacaaatccatgtagttcttatcaatcggttcgggattgaaccggtttaaattacgctcggtttggttcaaatttcaaattgagaaaaatatcc
gaataccgagtgaactcgtcgtcgttctttataacgagtcgactcaaaaacctctcttatcttaaaaaaagactaaaaccctgagtgtgagtgagagtat
gaacaaatcttcttcgtagcaccaacaaaaATGGTGTCCCTCTCTAATCAAACCAGATTCTCTTTCCACCCAAACAATCTTGTCGTTTCCGAGAAACGGC
GCCTGGGAATCTCAGGAGTCAATTTTCCCAGGAAGATCAAGTTGAAAATCACTTGCTTCGCCGCCGAGAGACCACGCCAGGAGAAACAGAAAAAGAAGAG
TCAGAGCCAGAGCACTAGCGACGCTGAGGCTGGAGTTGACCCTGTTGGGTTTCTCACCAGACTCGGAATTGCTGACAGAATCTTTGCTCAATTTCTCCGG
GAAAGgtataaaaatgtatcaactttgattttgaaaaaatgggcaacaaaaaaggatcaattttgattacagctgtgtataataagcctgttcgtttttc
tgtagGCATAAAGCTTTGAAAGACCTTAAAGATGAGATTTTCAAACGCCATTTCGATTTCAGAGATTTTGCTTCAGGgtatgtatcctcttggtcccagt
ttctttcaagagaagattttgatcctggattagtgactgatgtgtatccaaaatgctttgtctaatgaatagccttcttttcattgtagATTTGAGTTAT
TGGGGATGCATCGACATATGGAACACCGGGTTGACTTTATGGACTGGGGTCCAGgttctgtctcttgctctaatcttaaaatttcattagtcagttcatt
acttacatttggttctgaaacagaatgaatgatgttcagaaacttgatatgtatgttgggaagtttgagaatactaaccaatcctaaattcagGTTCTCG
TTATGGGGCTATAATCGGAGATTTCAATGGATGGTCGCCTACAGAAAATGCAGCTAGAGAGGGCCTATTTGGCCATGATGATTATGGGTATTGGTTCATT
ATTCTTGAAGATAAGTTAAGAGAGGgtgaagaaccagatgaactatactttcagcagtataactatgtcgatgactatgataagggtgatagtggtgtat
cagctgaagaaatcttccagaaagctaatgatgagtattgggaacctggtgaggatcggtttattaaaaaccgctttgaagttcctgccaaactatacga
gcagatgtttggcccgaacagtccacaaacgttagaagagcttggagatataccagatgcagaaacgagatataaacagtggaaagaggagcataaagat
gacccgccaagtaatctacctccatgcgacatcattgataaaggtcaaggaaagccatatgatatttttaatgtcgtgacatctccagaatggacaaaga
aattttatgagaaagaacctccgattccatattggttagagacacgtaaaggaagaaaggcatggttgcaaaaatatattccagCTGTTCCACATGGAAG
CAAGTATAGATTGTATTTCAATACTCCTGATGGACCGCTTGAACGAGTGCCTGCTTGGGCTACATATGTGCAACCAGgtatgacagcgtttggtatttct
atggttaggagtagcacatgaaatttaacaaatttcataacattcataaatatatggaagcaagcattcagtttttctttcatctgatgacacatgctta
gaagtatttgcacgaattgaacaaacggaaggagtttttttccattttttttactctctgcttttcatgtccagagttactagaactttgtaagcactaa
attaacagtcttactgaacaacatttttcagagaagctgtaaaacctttcttgtttctatacgctactgcttgatgtttctatgcagagatgcttttaaa
ataacgtttttgtatgtgtcatgttaatatttccacgataatgtatcttgtttattattaatgtattcatttttctctaaaatagAGGATGAAGGAAAGC
AAGCTTACGCAATTCATTGGGAACCTTCTCCTGAAGCTGCATACAAGTGGAAATATTCCAAGCCAAAAGTTCCAGAGTCCTTGCGCATATACGAATGTCA
TGTTGGAATCAGTGGGTCCGAGCCAAAAGTTTCAACTTTCGAAGAATTTACTAAGAAGgtacctcctaatgtattcattgtattcttacttgcaatctta
ttatgcacacatctcagtttttctgtggcctatcaaacatcagGTCCTTCCTCATGTGAAAAGAGCTGGATACAATGCAATCCAGTTGATTGGTGTCCCT
GAGCACAAGGATTATTTTACTGTTGGTTATAGGgtcagcaagctaaacttctagcacttaaactttcttttttactacttcttagtctggttatagggtc
agcatagctgttattcatagtcttatgttacctccagttcttatgattatatcgtctttggctttgctgtccactgtgcatgaaaggttctgaaggtctt
tgccgaatgattagtaaaaaatagacaatagtgtagaatagagagaattgatgaaaaatatggaacaagaagagaacaacttgatagaaataaagtggaa
agaaacaattttgattagtgggcattgcatatgtcatttagtcctgatcaggtccttttactagttacatttgaaaaaaataacagctatggaactacac
tcaagctaataaccagaattcctagtacataatatggcagattttacaaattattaaggaaactactgtcacttatctttaaaccgaatgaatggattta
tgaaaattattttttacctcatttgtcataattggtatccatcaccatttcagGTTACGAACTTCTTTGCTGCCAGTAGCCGATATGGCACGCCAGATGA
TTTCAAACGGTTGGTTGATGAGGCACATGgtaattatttctcgtctttcttgttttcttgatgttgtataaccatgtagtgacgaagcacatgatataca
gGCCTAGGACTTCTTGTCTTTTTGGACATCGTGCATTCTTATGCAGCAGCTGATCAGATGGTTGGGCTTTCTCTCTTCGATGGTTCAAATGATTGCTATT
TTCATTATGgtaatttccataatagtagttcactttttatttatttcatgagttcttgcatgaataaaagacttcttgcatgcctttattctaacgtgtt
gatgtccaagtcattggtatgtaactagaaagtatgcaccataattttcgtgttacaaccaatgttgttcataatcttgtgtacgtccaaagatgacgtc
aaacatgtctgacatagttgaatatgggcctgtctaaaaacacaatgttccattttcttcatcgtggaaatagGTAAAAGGGGGCATCACAAACACTGGG
GCACCCGGATGTTCAAATACGGTGATTTGGATGTTCTTCATTTTCTAATATCAAATTTGAACTGgtaaaggcagctacattattaagtggtttgttaata
atgtattggttgtactcactcttttggtatagtaggaaagtgttgcacactttttggttataggaatctgatgctgttgcatttggcttttgcagGTGGA
TTACAGAGTATCAAGTTGATGGTTACCAATTTCACTCGCTTGCCTCGATGATTTACACGCACAATGGGTTTGCTTCATTTAACAACGATTTGGATGAgta
aggcatctatgagactacacacctctttgtataattttgattacagttggtcaaactgttgacgtctttcctgttggcagCTATTGCAATCAGTATGTTG
ACCGAGATGCTCTGATGTACCTCATTTTGGCCAATGAAATCCTGCACGTTCAACATCCAAATATAATAACAATTGCTGAGGATgtaagctacacatcact
tttttttggtcatgcttttgtttaaagctattaaatattcatccttattgaattgctttgtcttaattgatagtattgagggggactagctactgacact
gctttcatcatagaatatcactaagtctctgaagtatatttctgggcataattgcttctaaattatctctagtttttagtcttgcaagtgttggttagta
gaattagtactgttactgccagttagtgaaaacttttcatgctttttccaaatatgctatatgtagataaaaagttgtgtaagagagtaacatgtaacat
gtatatatatgctttctcatgtacaacacatgacggtaaccatagtgtcgactggcttgtgtaagccaatttaaactggccatacgcaggaaaagaagtt
cttgacgaattatcttttacgaatgttagatgtatatcagtgggtaaaaagtttaagattcttattgacagaaaaattgcattaatttttctgtcgatga
tctgaatcgttgctcgttgggtttcactatctacccacttatagaacttaacaaggtttccttttgcttttagGCAACATATTACCCTGGGTTGTGTGAG
CCAGTTTCTCAAGGTGGACTTGGATTTGATTATTATGTGAATCTTTCTGCGTCAGAAATGTGGGTTTCTCTCCTCGACAATGTACCGGATAATGAATGGA
GCATGAGCAAGgttatagcttttacgtttccattgttatatatggcagaacaagacttttgtcctgttgtctgatgttcagCCAGTGCTACAGATTGTCA
GTACATTGGTGGCTAACAAAGAGTATGCAGACAAGATGCTCAGCTATGCCGAAAATCACAACCAAgtatctacgttgtaactttagtttttcatttctac
tacacaactaaacttttatgttgcattgacattgttgtttattctacttttcctttccaccagTCCATATCAGGAGGGCGTTCATTTGCTGAAATCTTAT
TCGGTGGAGTCGATAATGGTTCTCCTGGAGGAAAAGAATTGCTAGACAGGGGAATTTCACTACATAAGgtatccctttcttagtcctttacgcactactt
cccgaacttagcatacctctatcctgatgagaacacaactgtagaataagatttgatattattttgatttttgcattgaaaagtgaaaatatgaaatctg
gaagattgtctaattattgccctctgttgtcttttatctatattgtttcacttatattctgaaagctaacgtttcttgtcattgcttggacgaccagATG
ATTAGACTGATTACTTTTACAAGTGGTGGCCGTGCATACCTCAATTTCATGGGAAATGAATTTGGACATCCTGAGgtaaatctacaaactcccactgttc
taaagacatcatcttcatgtttctgattctgacgtttcatccttgtgtcagAGGGTTGAGTTTCCAACGCAGAGCAATAACTTCTCGTTTTCACTTGCTA
ACCGCCGCTGGGACCTGCTGGAAAGTGGAGTGCATCATCACTTGTTTTCCTTTGACAAGgtatcagtcttaccattaccacctgtttacaaaagtgcagc
ttccatatccatgattttgttagcatctctgaaaattttcttccttaaatttcctagcaatttaatactgtattagtctatgaaggcggcaaataaatgt
tatcaagccgagaaagttgtcagaactcgaacttaaaaaactttactgccgatatcatatcagaagaaactaaatgcttgatttcttattgaagttttaa
ttgtatatctttaaaatgtaaggactagaaaatgttccaagcatatatcctcttgtatcagaagagatgctttttagctactgatctcgttctttatttg
tagGAGCTAATGGACTTGGATAAAAGCAAGGGTATCCTTTCAAGAGGTCTGCCCAGCATCCACCATGTGAATGATGCAAATATGgtaattgaatcctttt
tctcttttattttccactgaaaattgattgggttcagggtctgaaaaatgagttcctggcattctttggcatgctagtgtttgtatatgcctttatatac
gatattattcagctaatgcggaaaataaataaaccaactgtcttcctgcaacatttgggaattctccaaatacatttatagaattataaaataacagtgt
aaacctgagcaaaatgagactacctaaagtaagcatcagaaatgaaagagtttgtacaatgagcctgcttactagtcaactaattgttctctacagGTGA
TTTCTTTCTCAAGGGGTCCTTTCCTATTTATCTTTAACTTCCACCCATCAAATTCATATGAAAAGTATGATGTCGGTGTAGAGGAAGCTGGTGAATATAC
Cgtaagttttttccattctctgtttagatattgcacccatcatctatcatctttaataagtgtctgtcaatgaaaccactgctttgctctccatgaaaca
cagttgctaccgcgtttttactcgttgatagaacaatgaactccactataaggttgttcaatgcgtttcacctgagaatattacctggattttgcagATG
ATACTGAACTCCGATGAAGTAAAGTATGGGGGTCAAGGGATTGTAACAGAAGACCACTATCTTCAACGGTCAATTAGCAAGAGgtttgtaattttagttt
aatgttatagtatcctacttagcaaagatataaggatgttatctgttcatcattttgaaccgaatccacatttgtgcagAATTGATGGTCAAAGAAATTG
CTTAGAGGTGTTTTTGCCTAGCCGGACTGCCCAAgtcagctctctctctctctctccttcttttgctctctctctatgctatctactgccatgagaattg
attcatagatgcattcaacctgtcatatgttgattcaaactccatttttatattctcaggtttacaagttgacccggatcctccgaatatgatcct
ctctctttgtatgccacatacaaacaccaagcatgtttgtctgagagGCTAGGTATGAACCCAAGGTCACTACAAGAGGTGATGTAATATTAACATGGCT
CGTCCAAGCGATGATGGCAGTTAAGAAGATTATCAATCACGCCTTCACAGAGGTGCAATAAaagaacaagcagttcataaagagaatcccaggaagacca
attcatctcgtggtgtagttcaagcaaacaaatgttttgtatcaaatcagctgcatcatgtaacgagaagttaacacatgaacctcttaatggtgtaggc
cgactgatgttatgtagtaaatttctaggaggtgctcaagtttgtgtatttaagaaatctttggtagttggtatatatagttttgttaatgcatttctcg
acaaaatcaatgctttccatttctcgacaaaacaattgctttccacaagagaagtcatccagtagcttagatatattgtttacctagttaaaaaaagtcc
aagg

Border_1 (48882_LBU)

GCCTTTTCAGAAATGGATAAATAGCCTTGCTTCCTATTATATCTTCCCAAATTACCAATACATTACACTAGCATCTGAATTTCATAACCAATCTCGATAC
ACCAAATCGAATTCAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCATATGACAGGTTGAATGCATCTATGAATCAA
TTCTCATGGCAGTAGATAGCATAGAGAGAGAGCAAAAGAAGGAGAGAGAGAGAGAGAGCTGACTTGGGCAGTCCGGCTAGGCAAAAACACCTCTAAGCAA
TTTCTTTGACCATCAATTCTGCACAAATGTGGATTCGGTTCAAAATGATGAACAGATAACATCCTTATATCTTTGCTAAGTAGGATACTATAACATTAAA
CTAAAATTACAAACCTCTTGCTAATTGACCGTTGAAGATAGTGGTCTTCTGTTACAATCCCTTGACCCCCATACTTTACTTCATCGGAGTTCAGTATCAT
CTGCAAAATCCAGGTAATATTCTCAGGTGAAACGCATTGAACAACCTTATAGTGGAGTTCATTGTTCTATCAACGAGTAA

Start of BLAST Alignment:NT 6749 / Intron 21 of 21
First 15 bp Perfect Match:NT 6699 / Intron 21 of 21
BLAST Details:
Query Sequence: 48882_LBU 
Length: (580 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At3g20440 68416.t02310 glycoside hydrolase family 13 prot...  1816  2.1e-76   1
At5g17130 68418.t01820 hypothetical protein                    183  0.89      1


>At3g20440 68416.t02310 glycoside hydrolase family 13 protein similar to
           1,4-alpha-glucan branching enzyme [Solanum tuberosum] GI:1621012,
           1,4-alpha-glucan branching enzyme (EC 2.4.1.18) from [Homo sapiens]
           SP|Q04446, {Solanum tuberosum} SP|P30924; contains Pfam profiles:
           PF00128 Alpha amylase catalytic domain, PF02922 Isoamylase
           N-terminal domain
        Length = 6927

  Minus Strand HSPs:

 Score = 1816 (278.5 bits), Expect = 2.1e-76, P = 2.1e-76
 Identities = 406/467 (86%), Positives = 406/467 (86%), Strand = Minus / Plus

Query:   580 TTACTCGTTGATAGAACAATGAACTCCACTATAAGGTTGTTCAATGCGTTTCACCTGAGA 521
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  6288 TTACTCGTTGATAGAACAATGAACTCCACTATAAGGTTGTTCAATGCGTTTCACCTGAGA 6347

Query:   520 ATATTACCTGGATTTTGCAGATGATACTGAACTCCGATGAAGTAAAGTATGGGGGTCAAG 461
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  6348 ATATTACCTGGATTTTGCAGATGATACTGAACTCCGATGAAGTAAAGTATGGGGGTCAAG 6407

Query:   460 GGATTGTAACAGAAGACCACTATCTTCAACGGTCAATTAGCAAGAGGTTTGTAATTTTAG 401
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  6408 GGATTGTAACAGAAGACCACTATCTTCAACGGTCAATTAGCAAGAGGTTTGTAATTTTAG 6467

Query:   400 TTTAATGTTATAGTATCCTACTTAGCAAAGATATAAGGATGTTATCTGTTCATCATTTTG 341
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  6468 TTTAATGTTATAGTATCCTACTTAGCAAAGATATAAGGATGTTATCTGTTCATCATTTTG 6527

Query:   340 AACCGAATCCACATTTGTGCAGAATTGATGGTCAAAGAAATTGCTTAGAGGTGTTTTTGC 281
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  6528 AACCGAATCCACATTTGTGCAGAATTGATGGTCAAAGAAATTGCTTAGAGGTGTTTTTGC 6587

Query:   280 CTAGCCGGACTGCCCAAGTCAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAT 221
             ||||||||||||||||||||||                                    ||
Sbjct:  6588 CTAGCCGGACTGCCCAAGTCAGCTCTCTCTCTCTCTCTCCTTCTTTTGCTCTCTCTCTAT 6647

Query:   220 GCTATCTACTGCCATGAGAATTGATTCATAGATGCATTCAACCTGTCATATGACAACTTA 161
             ||||||||||||||||||||||||||||||||||||||||||||||||||||   | || 
Sbjct:  6648 GCTATCTACTGCCATGAGAATTGATTCATAGATGCATTCAACCTGTCATATGTTGA-TTC 6706

Query:   160 ATAACAC-ATTGCGGACGTTTTTAA-TGTACTGAATTAACGCCGAAT 116
             | ||| | |||    |  || | |  | |||  | || || ||| ||
Sbjct:  6707 A-AACTCCATTTTT-ATATTCTCAGGTTTACA-AGTTGAC-CCGGAT 6749


>At5g17130 68418.t01820 hypothetical protein
        Length = 563

  Minus Strand HSPs:

 Score = 183 (33.5 bits), Expect = 2.2, P = 0.89
 Identities = 143/246 (58%), Positives = 143/246 (58%), Strand = Minus / Plus


Border_2 (48882_RBD)

AACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTCGAAATTAACCCTCACTAAAGGGAACAAAAGCTGGAGCTCGCTTGAGCTTGGAT
CAGATTGTCGTTTCCCGCCTTCAGTTTATGCATGCTAGCATATGCCATGGAAACTATCAGTGTTTACGCTTTCATATGTAGTCCGAATATGATCCTCTCT
CTTTGTATGCCACATACAAACACCAAGCATGTTTGTCTGAGAGGCTAGGTATGAACCCAAGGTCACTACAAGAGGTGATGTAATATTAACATGGCTCGTC
CAAGCGATGATGGCAGTTAAGAAGATTATCAATCACGCCTTCACAGAGGTGCAATAAAAGAACAAGCAGTTCATAAAGAGAATCCCAGGAAGACCAATTC
ATCTCGTGGTGTAGT

Start of BLAST Alignment:NT 6719 / Intron 21 of 21
First 15 bp Perfect Match:NT 6751 / Intron 21 of 21
BLAST Details:
Query Sequence: 48882_RBD 
Length: (415 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At3g20440 68416.t02310 glycoside hydrolase family 13 prot...   892  1.6e-34   1
At3g07400 68416.t00797 lipase class 3 family protein cont...   172  0.9995    1


>At3g20440 68416.t02310 glycoside hydrolase family 13 protein similar to
           1,4-alpha-glucan branching enzyme [Solanum tuberosum] GI:1621012,
           1,4-alpha-glucan branching enzyme (EC 2.4.1.18) from [Homo sapiens]
           SP|Q04446, {Solanum tuberosum} SP|P30924; contains Pfam profiles:
           PF00128 Alpha amylase catalytic domain, PF02922 Isoamylase
           N-terminal domain
        Length = 6927

  Plus Strand HSPs:

 Score = 892 (139.9 bits), Expect = 1.6e-34, P = 1.6e-34
 Identities = 194/209 (92%), Positives = 194/209 (92%), Strand = Plus / Plus

Query:   151 AAACTATCAGTGTTTACGCTTTCATATGTAG--TCCGAATATGATCCTCTCTCTTTGTAT 208
             | | | |||| ||||||   || |   | |   |||||||||||||||||||||||||||
Sbjct:  6720 ATATTCTCAG-GTTTACAAGTTGACCCGGATCCTCCGAATATGATCCTCTCTCTTTGTAT 6778

Query:   209 GCCACATACAAACACCAAGCATGTTTGTCTGAGAGGCTAGGTATGAACCCAAGGTCACTA 268
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  6779 GCCACATACAAACACCAAGCATGTTTGTCTGAGAGGCTAGGTATGAACCCAAGGTCACTA 6838

Query:   269 CAAGAGGTGATGTAATATTAACATGGCTCGTCCAAGCGATGATGGCAGTTAAGAAGATTA 328
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  6839 CAAGAGGTGATGTAATATTAACATGGCTCGTCCAAGCGATGATGGCAGTTAAGAAGATTA 6898

Query:   329 TCAATCACGCCTTCACAGAGGTGCAATAA 357
             |||||||||||||||||||||||||||||
Sbjct:  6899 TCAATCACGCCTTCACAGAGGTGCAATAA 6927


>At3g07400 68416.t00797 lipase class 3 family protein contains Pfam profile
           PF01764: Lipase
        Length = 5414

  Plus Strand HSPs:

 Score = 172 (31.9 bits), Expect = 7.6, P = 0.9995
 Identities = 136/224 (60%), Positives = 136/224 (60%), Strand = Plus / Plus