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At1g67440 SYNGENTA 54334

Genomic Sequence (from TAIR)
TAGAGATGTTATCGGAACCCTAGACAAAATCTCTTCTACCAAATCATTCGGAAGATCAGACATCATCATCGTCATtttttcaaatgaaaaattagggttg
aactatcacgtcttttccacacttttaagatttattctgagattgttcacagcataatatggattaatcttctaggaagccttatattgtgtaacagatt
aattgtacagatgcctttcatttgcagacttattaaagatgaaatgattaataagagcagatttgttctttttaatcatcagtcatcatcaatgtcaata
ttaataagccatcaaatcaatgaaagacttggaaaaatatctcaaacttacttacaccttcccacacacatttacggtcgattatatatgcaatagatgg
gccctaattttgggcccataacacttatcacaaaaggcccatcactttaaatcttcttcataattcactgctctcttcctcactacacagttttctattc
ttctagaatttgcttatgcagacattttcaatttcactcctccgtcactcttctccgttcctccgtcacaccgcgatttttcacggcggagttggagt
tagattcaaattccttcgcagcttttctcctctctcagcaagacgagataatcccgatgtctccagaaaaccccaaccgagcaaaaatATGCTCAGAGC
GAAGCACATCGGTAAAAATTACTCCTCCTCCTTGTCACCAGTTCTCTCGCCGGAGCATAAACCTTCGCTCTTAGAGTCTCAAGCTATTGGTACTGTCGCA
ACAGCTCAAGCGAACTTTATGCGCGTCATTGTTCAAGATGTAGCTAATTCTGTGACGAGTGATGATGATAATGATTCTTCAAAGACTGGTGTTGAATTGC
TTTGTGTGGTGAGAGCTGTGTTGAAGAAAATACGAAGGAGAGTTTTAGTTGGAGATAAGGTTCTTGTTGGATCTATTGATTGGGTTGATAGAAGAGGAAT
GATTGAGAATGTGTTTCATCGACGTTCGGAGATTTTGGATCCACCTGTTGCGAACGTTGATCATTTGCTTGTTCTTTTCTCTTTGGATCAACCGAAACTT
GAGCCGTTTACTCTTACTAGGTTCTTGGTGGAAGCTGAATCTACTCGTATTCCATTAACACTTGCTTTGAATAAAACTGAACTCATTAGTGAAGAGgtaa
ggaggatgaagaaattgtattttagatcactttgttggtttagggattgattagaatcataaacttgtttacagGAATTGGAAACTTGGAAGATAAGGTT
GCGTGGATGGAACTATGAACCATTGTTTTGTAGTGTGGGAACTAAAGATGGACTTGATGATATTGCGTTTGTTCTGAGAGATCAGACTTCTGTGATTGTT
GGACCTAGTGGTGTTGGAAAGTCGAGTTTAATCAACGTATTGAGGAGTAATCATGGTGGTGGTGTGGTGGAAGATGAGAATTGGTTTGAGCCTgtaaggt
tcttttgtgttatttcattttacagcttaagttttgtttgccagattcttatgagttgagcttttgtgatattcagATGTTAGGTAATAAGTGGTTTGAT
GATCAGCGAGTAGGGGAAGTTTCGAGTAGAAGTGGTAGAGGTAAACATACAACACGAAATGTATCGCTACTGCCGGTTTCTGAAGGTGGTTACCTCGCTG
ATACTCCTGGCTTTAACCAGCCTAGTTTGCTGAAAGTAACGAAGCATTCACTAGCTCACTGTTTTCCTGAGgtttgttctttgtacccttttgcttgtgc
caatctcccttattggctgactgtgacgatgtgttactttttaattgtttcttttagATACGGAACATGATTGAGAGCGAAAAATGTGGATTCAGAGACT
GCTTGCATATTGGGGAACCAGGATGTGTTGTGAAAGGTGACTGGGAAAGGTATCCTTACTACTTACAATTGCTTGATGAGATCAGAATCAGGGAAGAATT
TCAGCTTAGGACTTTTGGAACCAAAAGGGAAGATGATGTTAGgtattgttgggatttctcctgaaagaacagctttatttttagattgagattggtcgca
gtcactaatgttgtttactttggaatgatacttgacacagGTACAAGGTGGGAGACATGGGTGTGAAACATGCTGAACCACGGTTAATGCCTAAGAAGCA
TAGGAGAGAGTCAAGGAAGAAAACGAAACAGACAATGATCAGTGAGCTGGATGAGTTCGAAGATGAAGACAGTGATTTGTACATAGAGAACGACCCAATC
GTCCAAGCCATTGAGAATGAGAATAAAAGACAATGAgagaactcaagtttccactataactaagtcacatgatgtaaaagattgtacagatctttttaag
ttctcagctcttttgtttgtctgaattattgactaatcgaactatcgaactaaccaaaacttgcagaaccatgatcactgatttcataagtcatagaacg
aaaatttccaagataaatctatccattgtcttacaaaacgattcgagctaaagaaaaaagagaacaaaatctcgttcaagaacacaagggacatgaaaat
aaagaaagaaagcTTAAGCTGCTGATTTCTTGGACTTGGCAGCCAACTGAGTCTCCGGctacaattatcaaaaacaaatacgaaatgagaacactgttgt
ta

Border_1 (54334_LBD)

GCCTTTTCAGAAATGGATAAATAGCCTTGCTTCCTATTATATCTTCCCAAATTACCAATACATTACACTAGCATCTGAATTTCATAACCAATCTCGATAC
ACCAAATCGAATTCAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTCTGCATAAGCAAATTC
TAGAAGAATAGAAAACTGTGTAGTGAGGAAGAGAGCAGTGAATTATGAAGAAGATTTAAAGTGATGGGCCTTTTGTGATAAGTGTTATGGGCCCAAAATT
AGGGCCCATCTATTGCATATATAATCGACCGTAAATGTGTGTGGGAAGGTGTAAGTAAGTTTGAGATATTTTTCCAAGTCTTTCATTGATTTGATGGCTT
ATTAATATTGACATTGATGATGACTGATGATTAAAAAGAACAAATCTGCTCTTATTAATCATTTCATCTTTAATAAGTCTGCAAATGAAAGGCATCTGTA
CAATTAATCTGTTACACAATATAAGGCTTCCTAGAAGATTAATCCATATTATGCTGTGAACAATCTCAGAATAAATCTTAAAAGTGTGGAAAAGACGTGA
TAGTTCAACCCTAATTTTTCATTTGAAAAAATGACGATGATGATGTCTGATCTTCCGAATGATTTGGTAGAAGAGATTTTGTCTAGGGTTCCGATAACAT
CTCTAGGAGCAGTGAGATCTACTTGCAAACGATGGAACGGTTTATCCAAAGATCGTATTGTATGTAAAGGAGATGCAAATCAACAGTTTACAGGGTTCAC
GAGATATGTGAACGAATCTACGGTTTGTTCAATGAGACTTGATCTTAATAAATCCAAA

Start of BLAST Alignment:<162/ 5’ UTR
First 15 bp Perfect Match:<162 / 5’ UTR
BLAST Details:
Query Sequence: 54334_LBD Border #1 
Length: (858 letters) 
Database: TIGR AGI BAC Sequences (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

T1F15 51036                                                   3378  2.0e-146  1
MPN9 60486                                                     810  2.1e-30   1
MAL21 60540                                                    760  3.8e-28   1
T32N4 67065                                                    732  7.0e-27   1

T1F15 51036
        Length = 55,721
  Minus Strand HSPs:
 Score = 3378 (512.9 bits), Expect = 2.0e-146, P = 2.0e-146
 Identities = 680/684 (99%), Positives = 680/684 (99%), Strand = Minus / Plus

Query:   858 TTTGGATTT-ATTAAGATCAAGTCTCATTGAACAAACCGTAGATTCGTTCACATATCTCG 800
             ||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 40997 TTTGGATTTCATTAAGATCAAGTCTCATTGAACAAACCGTAGATTCGTTCACATATCTCG 41056

Query:   799 TGAACCCTGTAAACTGTTGATTTGCATCTCCTTTACATACAATACGATCTTTGGATAAAC 740
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 41057 TGAACCCTGTAAACTGTTGATTTGCATCTCCTTTACATACAATACGATCTTTGGATAAAC 41116

Query:   739 CGTTCCATCGTTTGCAAGTAGATCTCACTGCTCCTAGAGATGTTATCGGAACCCTAGACA 680
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 41117 CGTTCCATCGTTTGCAAGTAGATCTCACTGCTCCTAGAGATGTTATCGGAACCCTAGACA 41176

Query:   679 AAATCTCTTCTACCAAATCATTCGGAAGATCAGACATCATCATCGTCATTTTTTCAAATG 620
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 41177 AAATCTCTTCTACCAAATCATTCGGAAGATCAGACATCATCATCGTCATTTTTTCAAATG 41236

Query:   619 AAAAATTAGGGTTGAACTATCACGTCTTTTCCACACTTTTAAGATTTATTCTGAGATTGT 560
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 41237 AAAAATTAGGGTTGAACTATCACGTCTTTTCCACACTTTTAAGATTTATTCTGAGATTGT 41296

Query:   559 TCACAGCATAATATGGATTAATCTTCTAGGAAGCCTTATATTGTGTAACAGATTAATTGT 500
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 41297 TCACAGCATAATATGGATTAATCTTCTAGGAAGCCTTATATTGTGTAACAGATTAATTGT 41356

Query:   499 ACAGATGCCTTTCATTTGCAGACTTATTAAAGATGAAATGATTAATAAGAGCAGATTTGT 440
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 41357 ACAGATGCCTTTCATTTGCAGACTTATTAAAGATGAAATGATTAATAAGAGCAGATTTGT 41416

Query:   439 TCTTTTTAATCATCAGTCATCATCAATGTCAATATTAATAAGCCATCAAATCAATGAAAG 380
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 41417 TCTTTTTAATCATCAGTCATCATCAATGTCAATATTAATAAGCCATCAAATCAATGAAAG 41476

Query:   379 ACTTGGAAAAATATCTCAAACTTACTTACACCTTCCCACACACATTTACGGTCGATTATA 320
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 41477 ACTTGGAAAAATATCTCAAACTTACTTACACCTTCCCACACACATTTACGGTCGATTATA 41536

Query:   319 TATGCAATAGATGGGCCCTAATTTTGGGCCCATAACACTTATCACAAAAGGCCCATCACT 260
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 41537 TATGCAATAGATGGGCCCTAATTTTGGGCCCATAACACTTATCACAAAAGGCCCATCACT 41596

Query:   259 TTAAATCTTCTTCATAATTCACTGCTCTCTTCCTCACTACACAGTTTTCTATTCTTCTAG 200
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 41597 TTAAATCTTCTTCATAATTCACTGCTCTCTTCCTCACTACACAGTTTTCTATTCTTCTAG 41656

Query:   199 AATTTGCTTATGCAGACAAATTGA 176
             ||||||||||||||||||  || |
Sbjct: 41657 AATTTGCTTATGCAGACATTTTCA 41680

 Score = 459 (74.9 bits), Expect = 9.3e-14, Sum P(2) = 9.3e-14
 Identities = 135/174 (77%), Positives = 135/174 (77%), Strand = Minus / Plus

Query:   718 ATCTCACTGCTCCTAGAGATGTTATCGGAACCCTAGACAAAATCTCTTCTACCAAATCAT 659
             |||||| ||||||||||||| |||| | |||||||||||  ||||||||||||| ||| |
Sbjct: 39162 ATCTCAATGCTCCTAGAGATATTATTGAAACCCTAGACAGTATCTCTTCTACCATATCCT 39221

Query:   658 TCGGAAGATCAGACATCATCATCGTCATTTTTTCAAATGAAAAATTAGGGTT----GAAC 603
              ||||||||| || |||||||||||| | ||  |||||||||| | ||||||    || |
Sbjct: 39222 CCGGAAGATCGGATATCATCATCGTCGTCTT--CAAATGAAAA-TCAGGGTTTTTTGACC 39278

Query:   602 TATCACGTCTTTTCCACACTTTTAAGATTT-ATTCTGAGAT-TGTTCACAGCAT 551
             ||||| || ||| | ||| ||||  ||||| ||| | | || ||  || | | |
Sbjct: 39279 TATCATGTATTTCCTACAGTTTTTCGATTTGATTAT-ATATATGCGCATAACTT 39331

 Score = 344 (57.7 bits), Expect = 1.7e-07, Sum P(2) = 1.7e-07
 Identities = 124/172 (72%), Positives = 124/172 (72%), Strand = Minus / Plus

Query:   858 TTTGGATTT-ATTAAGATCAAGTCTCATTGAACAAACCGTAGATTCGTTCACATATCTCG 800
             ||||||||  || |||||||| |||||||||||||||| ||||| | ||    ||||   
Sbjct: 39042 TTTGGATTCCATGAAGATCAAATCTCATTGAACAAACCTTAGATCCCTT----TATCA-- 39095

Query:   799 TGAACCCTGTAAACTGTTGATTTGCATCTCCTTTACATACAATACGATCTTTGGATAAAC 740
             |||| || | |||||||||  | || ||| |||||||||  |  ||| |||| |||||| 
Sbjct: 39096 TGAAACCAGCAAACTGTTGTCTAGCTTCTGCTTTACATATGACCCGAGCTTTAGATAAAT 39155

Query:   739 CGTTCCATCGTT-TGCAAGTAGA--TCTCACTGCT-CC-TAGAGATGTTATC 693
             |||||||||    |||   ||||  | | | ||   || |||| | || |||
Sbjct: 39156 CGTTCCATCTCAATGCTCCTAGAGATATTATTGAAACCCTAGACA-GT-ATC 39205

 Score = 128 (25.3 bits), Expect = 9.3e-14, Sum P(2) = 9.3e-14
 Identities = 144/256 (56%), Positives = 144/256 (56%), Strand = Minus / Plus

Query:   328 TCGATTATATATGCAATAGATGGGCCCTAATTTTGGGCCCATAACACTTATCA-CAAAAG 270
             ||||||  || |  | || ||| ||  ||| |||||||||||||| || ||   |     
Sbjct: 39302 TCGATTTGAT-TATA-TATATGCGCA-TAACTTTGGGCCCATAACTCTAATTTTCTTCTT 39358

Query:   269 GC--CCA-TCACTTTA-AAT-CTTCTTCATAATTCACTGCTCTCTTCCTCACTACACAGT 215
             ||  ||  |   | || ||  |  ||| ||| | | ||  | | ||| | |  | ||| |
Sbjct: 39359 GCTTCCCGTTTGTGTATAACACACCTT-ATA-TGCCCTCATAT-TTCATAAGGA-ACA-T 39413

Query:   214 TTTCTATTCTTCTAGAATTTGCTT-ATGCAGACAAATTGACGCTTAGACAACTTAATAAC 156
             ||| ||      ||  |   | || | | ||| |||   |     | ||||   || |||
Sbjct: 39414 TTTGTAGAAGATTATTACAAGATTCACGAAGAGAAAGA-ATAAAGACACAAGACAA-AAC 39471

Query:   155 ACATTGCGG-ACGTTTTTAATGTACTGAATTAA-CGCCGAATTGAATTCGATTTGGTGTA 98
             | |  |  | || | | |||   | |  || || |||   ||  |||  | ||  |||| 
Sbjct: 39472 AAAAAGATGCACCTCTCTAAAACA-TCTATAAAACGCTCTATATAAT--GTTTATGTGTT 39528

Query:    97 TCGAGATTGGTTATGA 82
             | | || || | || |
Sbjct: 39529 T-GCGACTG-TGATAA 39542


MPN9 60486
        Length = 81,035

  Minus Strand HSPs:

 Score = 810 (127.6 bits), Expect = 2.1e-30, P = 2.1e-30
 Identities = 342/513 (66%), Positives = 342/513 (66%), Strand = Minus / Plus

Border_2 (54334_RBU)

ATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGA
TTACGCCAAGCGCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGAGCTCGCTTGAGCTTGGATCAGATTGTCGTTTCCCGCCTTCAGTTTATGCATG
CTAGCATATGCCATGGAAACTATCAGTGCTAGCATATGCCGGTCGGGGAGCTGTTGGCGTCAATTGTGACGCTTAGACAACTATCAGTACATGCTAGCAT
ATAAACATGCATAAACTTCCGCCTTCAGTTTATGCATGCTAGCATATGCCATGGAGCGATGTCTCCAGAAAACCCCAACCGAGCAAAAATATGCTCAGAG
CGAAGCACATCGGTAAAAATTACTCCTCCTCCTTGTCACCAGTTCTCTCGCCGGAGCATAAACCTTCGCTCTTAGAGTCTCAAGCTATTGGTACTGTCGC
AACAGCTCAAGCGAACTTTATGCGCGTCATTGTTCAAGATGTAGCTAATTCTGTGACGAGTGATGATGATAATGATTCTTCAAAGACTGGTGTTGAATTG
CTTTGTGTGGTGAGAGCTGTGTTGAAGAAAATACGAAGGAGAGTTTTAGTTGGAGATAAGGTTCTTGTTGGATCTATTGATTGGGTTGATAGAAGAGGAA
TGATTGAGAATGTGTTTCATCGACGTTCGGAGATTTTGGATCCACCTGTTGCGAACGTTGATCATTTGCTTGTTCTTTTCTCTTTGGATCAACCGAAACT
TGAGCCGTTTACTCTTACTAGGTTCTTGGTGGAAGCTGAATCTACTCGTATTCCATTA

Start of BLAST Alignment:NT < 34 / 5’ UTR
First 15 bp Perfect Match:NT < 34 / 5’ UTR
BLAST Details:
Query Sequence: 54334_RBU 
Length: (858 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At1g67440 68414.t07035 expressed protein contains Pfam do...  2510  6.1e-109  1
At1g67460 68414.t07040 hypothetical protein contains Pfam...   870  1.8e-48   2
At5g61970 68418.t07184 signal recognition particle-relate...   210  0.46      1
At5g27500 68418.t02987 hypothetical protein hypothetical ...   196  0.89      1


>At1g67440 68414.t07035 expressed protein contains Pfam domain PF03193: Protein
           of unknown function, DUF258
        Length = 1916

  Plus Strand HSPs:

 Score = 2510 (382.7 bits), Expect = 6.1e-109, P = 6.1e-109
 Identities = 502/502 (100%), Positives = 502/502 (100%), Strand = Plus / Plus

Query:   357 CGATGTCTCCAGAAAACCCCAACCGAGCAAAAATATGCTCAGAGCGAAGCACATCGGTAA 416
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   135 CGATGTCTCCAGAAAACCCCAACCGAGCAAAAATATGCTCAGAGCGAAGCACATCGGTAA 194

Query:   417 AAATTACTCCTCCTCCTTGTCACCAGTTCTCTCGCCGGAGCATAAACCTTCGCTCTTAGA 476
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   195 AAATTACTCCTCCTCCTTGTCACCAGTTCTCTCGCCGGAGCATAAACCTTCGCTCTTAGA 254

Query:   477 GTCTCAAGCTATTGGTACTGTCGCAACAGCTCAAGCGAACTTTATGCGCGTCATTGTTCA 536
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   255 GTCTCAAGCTATTGGTACTGTCGCAACAGCTCAAGCGAACTTTATGCGCGTCATTGTTCA 314

Query:   537 AGATGTAGCTAATTCTGTGACGAGTGATGATGATAATGATTCTTCAAAGACTGGTGTTGA 596
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   315 AGATGTAGCTAATTCTGTGACGAGTGATGATGATAATGATTCTTCAAAGACTGGTGTTGA 374

Query:   597 ATTGCTTTGTGTGGTGAGAGCTGTGTTGAAGAAAATACGAAGGAGAGTTTTAGTTGGAGA 656
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   375 ATTGCTTTGTGTGGTGAGAGCTGTGTTGAAGAAAATACGAAGGAGAGTTTTAGTTGGAGA 434

Query:   657 TAAGGTTCTTGTTGGATCTATTGATTGGGTTGATAGAAGAGGAATGATTGAGAATGTGTT 716
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   435 TAAGGTTCTTGTTGGATCTATTGATTGGGTTGATAGAAGAGGAATGATTGAGAATGTGTT 494

Query:   717 TCATCGACGTTCGGAGATTTTGGATCCACCTGTTGCGAACGTTGATCATTTGCTTGTTCT 776
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   495 TCATCGACGTTCGGAGATTTTGGATCCACCTGTTGCGAACGTTGATCATTTGCTTGTTCT 554

Query:   777 TTTCTCTTTGGATCAACCGAAACTTGAGCCGTTTACTCTTACTAGGTTCTTGGTGGAAGC 836
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   555 TTTCTCTTTGGATCAACCGAAACTTGAGCCGTTTACTCTTACTAGGTTCTTGGTGGAAGC 614

Query:   837 TGAATCTACTCGTATTCCATTA 858
             ||||||||||||||||||||||
Sbjct:   615 TGAATCTACTCGTATTCCATTA 636


>At1g67460 68414.t07040 hypothetical protein contains Pfam domain PF03193:
           Protein of unknown function, DUF258
        Length = 1992

  Plus Strand HSPs:

 Score = 870 (136.6 bits), Expect = 1.8e-48, Sum P(2) = 1.8e-48
 Identities = 238/306 (77%), Positives = 238/306 (77%), Strand = Plus / Plus