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At3g20400 SYNGENTA 64071

Genomic Sequence (from TAIR)
ggcgactctattggcgcatgcatgaacttgcgcccacagaagaatctaaatgttttataaaataaaataaaagcaagaaaattgaatggagaaattaatg
cagttttgaaatatgaaaatgggaagggatgggataagcttgagattgaaatatatccaatttacattccactacgatctgaatgagttgtttattgcca
tttacattctagtcattatagtggtagcagtaaacttccaatcttggattcttaatctagcaaaaagaagctcttcattaaccaatgtttatcaatgagt
ttggatagactaatttttaccgcatttgtttgttagctcaactagatttatgtttcatataggctatgacacagacttgtatagtaagaagactagcata
cattagaaATGGAGATCTGGGTTACAACTAAGATTGAGCCCAACATGTTGTCGTGGGGCAGCAAGGTCTTCTTATCAGTGGATATGACACCACTCACTGG
CAACGATTTTATGTTTTCGTTTATGGCTACAAGTTTCTTCATTGATGAAGAGAAGAAAATCGCTGTGGTTTTTAATCAAAGCAAAGACAGGAAGCACAAC
ACAGCTTTCATCATTGGACAGGATGGATCCTTGAAAGAAGTGGATCTTGGAGAAGTTCGAAACAGAGATCTCAAACCACTTGTGTCCTCTTATGTTCCAA
GTTCAATGCAACTTGAATAGtgcattttacaaaacccataatctatttcttgcacttttacttgtttctttttctcttttgtcatcttcttctttgaa
caatatatagaaatttaattcgtctctcatacttctttttgtttgccatatttgacttcgttttgttgcctttagttgtttaatttacttcttctgttg
tagtagactcattgctaaatctctgtttctcttctaacatttgttatgtttgtgttcttgcaaataggcagctctgctgttgtaatttatgtagaacaga
caacagagtaagctgcgtttaactttgaaatttgcaagtacgcatgcttagatttgagtttcccattttaacttttgtccttgtcagttttacaaagtgc
aagtggctgctaggctgacacgtagaagattgaatgatttctcggagttaagttttgtcctttgatactccctggtcttaaagcatacttacagagtaac
cgtagctgaatatcaacctcaagcaagtcatggaaccatacaccttccttcaatccaccagttttgggctggttagcgctctcctagatttatgtttcat
ataagctaaccattaaaagtttaagagaagcttgtgtagtaagaagacaagctaaatgagttctcagtagccttaattcttcttctttttttgactaaat
atgagcacttatagatgaagactagtaatgcattgattatgagaatactaaaaagttaagatgaataatcaaaaaaattatttggttagtattaaaatct
tcaaatgaaacttagtcttaagatttgttgagatctttcatactatcgaagtcatgtagagtggaggtacgtagccaggcctaggagaagagaagagaag
agaaggagaagcaagctaagaaactgaaagcctaaaaacttttgaatgttgatgattaaaaaagaatagatacatgctaacagcttatgcatttttgaaa
tagtttttgttaactgtcgtgtagcttgtgtgtaaatatgtcgacgacaagtcaatgatgtcacacacactacacaaaacaaaacactgcttcaaactac
cttcaacttcgagtccattactataagcaaaagtcccaaatcaaaacatcaattttcttgttcttgtcagctactcaaacctcaacatgttacatatatt
ttttcagataaaacaaatcattctcatcgttcttatctgaccaggaataattcaatggaagtatgagtttgactcggtttccttttgatattagtcgtac
ttttcaacattttacctagatagagccgtcctcttataattattcatcatttcatgcttctcatgttacatttctgcaatttttcaactctttgatttta
tataatcatttgtttcctttcttaatcaaatccatctggctaacattatttagcttgatgcaattaaggtatattatctaatgaggtgatgcttccacgt
ctttatattattataatccctcaacaattttaaaaaaagatcctgactttcaattttctctcttgtttcttcttttgatcatcttcaacaaaaaaaagtt
acgatctttctctccgggtcatcggaatttgagctagcttagctaaagttccgatctttcctctctgggtcgtcggaatttgagctttttaaaatcATGG
GAA

Border_1 (64071_LB2)

GCCTTTTCAGAAATGGATAAATAGCCTTGCTTCCTATTATATCTTCCCAAATTACCAATACATTACACTAGCATCTGAATTTCATAACCAATCTCGATAC
ACCAAATCGAATTCAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCCGCAATATGGGTTTTGTAAAATG
CACTATTCAAGTTGCATTGAACTTGGAACATAAGAGGACACAAGTGGTTTGAGATCTCTGTTTCGAACTTCTCCAAGATCCACTTCTTTCAAGGATCCAT
CCTGTCCAATGATGAAAGCTGTGTTGTGCTTCCTGTCTTTGCTTTGATTAAAAACCACAGCGATTTTCTTCTCTTCATCAATGAAGAAACTTGTAGCCAT
AAACGAAAACATAAAATCGTTGCCAGTGAGTGGTGTCATATCCACTGATAAGAAGACCTTGCTGCCCCACGACAACATGTTGGGCTCAATCTTAGTTGTA
ACCCAGATCTCCATTTCTAATGTATGCTAGTCTTCTTACTATACAAGTCTGTGTCATAGCCTATATGAAACATAAATCTAGTTGAGCTAACAAACAAATG
CGGTAAAAATTAGTCTATCCAAACTCATTGATAAACATTGGTTAATGAAGAGCTTCTTTTTGCTAGATTAAGAATCCAAGATTGGAAGTTTACTGCTACC
ACTATAATGACTAGAATGTAAATGGCAATAAACAACTCATTCAGATCGTAGTGGAATGTAAATTGGATATATTTCAATCTCAAGCTTATCCCATCCCTTC
CCATTTTCATATTTCAAAACTGCATTAATTTCTCCATTCAATTTTCTTGCTTTTATTTTATTTTATAAAACA

Start of BLAST Alignment:NT > 119 / Downstream
First 15 bp Perfect Match:NT > 20 / Downstream
BLAST Details:
Query Sequence: 64071_LB2 Border #1 
Length: (872 letters) 
Database: TIGR AGI BAC Sequences (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

MQC12 60512                                                   3462  3.3e-150  1
MLM24 60465                                                   1181  3.6e-47   1
MWI23 60463                                                    939  3.1e-36   1
MLD14 60483                                                    848  4.0e-32   1


MQC12 60512
        Length = 87,799

  Minus Strand HSPs:

 Score = 3462 (525.5 bits), Expect = 3.3e-150, P = 3.3e-150
 Identities = 740/792 (93%), Positives = 740/792 (93%), Strand = Minus / Plus

Query:   872 TGTTTTATAAAATAAAATAAAAGCAAGAAAATTGAATGGAGAAATTAATGCAGTTTTGAA 813
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 51010 TGTTTTATAAAATAAAATAAAAGCAAGAAAATTGAATGGAGAAATTAATGCAGTTTTGAA 51069

Query:   812 ATATGAAAATGGGAAGGGATGGGATAAGCTTGAGATTGAAATATATCCAATTTACATTCC 753
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 51070 ATATGAAAATGGGAAGGGATGGGATAAGCTTGAGATTGAAATATATCCAATTTACATTCC 51129

Query:   752 ACTACGATCTGAATGAGTTGTTTATTGCCATTTACATTCTAGTCATTATAGTGGTAGCAG 693
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 51130 ACTACGATCTGAATGAGTTGTTTATTGCCATTTACATTCTAGTCATTATAGTGGTAGCAG 51189

Query:   692 TAAACTTCCAATCTTGGATTCTTAATCTAGCAAAAAGAAGCTCTTCATTAACCAATGTTT 633
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 51190 TAAACTTCCAATCTTGGATTCTTAATCTAGCAAAAAGAAGCTCTTCATTAACCAATGTTT 51249

Query:   632 ATCAATGAGTTTGGATAGACTAATTTTTACCGCATTTGTTTGTTAGCTCAACTAGATTTA 573
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 51250 ATCAATGAGTTTGGATAGACTAATTTTTACCGCATTTGTTTGTTAGCTCAACTAGATTTA 51309

Query:   572 TGTTTCATATAGGCTATGACACAGACTTGTATAGTAAGAAGACTAGCATACATTAGAAAT 513
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 51310 TGTTTCATATAGGCTATGACACAGACTTGTATAGTAAGAAGACTAGCATACATTAGAAAT 51369

Query:   512 GGAGATCTGGGTTACAACTAAGATTGAGCCCAACATGTTGTCGTGGGGCAGCAAGGTCTT 453
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 51370 GGAGATCTGGGTTACAACTAAGATTGAGCCCAACATGTTGTCGTGGGGCAGCAAGGTCTT 51429

Query:   452 CTTATCAGTGGATATGACACCACTCACTGGCAACGATTTTATGTTTTCGTTTATGGCTAC 393
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 51430 CTTATCAGTGGATATGACACCACTCACTGGCAACGATTTTATGTTTTCGTTTATGGCTAC 51489

Query:   392 AAGTTTCTTCATTGATGAAGAGAAGAAAATCGCTGTGGTTTTTAATCAAAGCAAAGACAG 333
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 51490 AAGTTTCTTCATTGATGAAGAGAAGAAAATCGCTGTGGTTTTTAATCAAAGCAAAGACAG 51549

Query:   332 GAAGCACAACACAGCTTTCATCATTGGACAGGATGGATCCTTGAAAGAAGTGGATCTTGG 273
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 51550 GAAGCACAACACAGCTTTCATCATTGGACAGGATGGATCCTTGAAAGAAGTGGATCTTGG 51609

Query:   272 AGAAGTTCGAAACAGAGATCTCAAACCACTTGTGTCCTCTTATGTTCCAAGTTCAATGCA 213
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 51610 AGAAGTTCGAAACAGAGATCTCAAACCACTTGTGTCCTCTTATGTTCCAAGTTCAATGCA 51669

Query:   212 ACTTGAATAGTGCATTTTACAAAACCCATATTGCGGACGCTTAGACAACTTAATAACACA 153
             |||||||||||||||||||||||||||||| | |     |||  ||   ||| |      
Sbjct: 51670 ACTTGAATAGTGCATTTTACAAAACCCATAAT-CTATTTCTTGCACTT-TTACTTGTTTC 51727

Query:   152 TTGCGGACGTTTTTAATGTACTGAATT-AACGCCGAATTGAATTCGATTTGGTGTATCGA 94
             ||     | ||| | || | ||   || |||     || ||| |  | || || | || |
Sbjct: 51728 TTTTTCTCTTTTGTCATCTTCTTCTTTGAACAATATATAGAAATTTAATTCGTCTCTC-A 51786

Query:    93 GA-TTGGTTATG 83
              | ||  || ||
Sbjct: 51787 TACTTCTTTTTG 51798


MLM24 60465
        Length = 87,487

  Plus Strand HSPs:

 Score = 1181 (183.2 bits), Expect = 3.6e-47, P = 3.6e-47
 Identities = 393/544 (72%), Positives = 393/544 (72%), Strand = Plus / Plus


Border_2 (64071_LB1)

GCCTTTTCAGAAATGGATAAATAGCCTTGCTTCCTATTATATCTTCCCAAATTACCAATACATTACACTAGCATCTGAATTTCATAACCAATCTCGATAC
ACCAAATCGAATTCAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTACACCAATCTATTT
CTTGCACTTTTACTTGTTTCTTTTTCTCTTTTGTCATCTTCTTCTTTGAACAATATATAGAAATTTAATTCGTCTCTCATACTTCTTTTTGTTTGCCATA
TTTGACTTCGTTTTGTTGCCTTTAGTTGTTTAATTTACTTCTTCTGTTGTAGTAGACTCATTGCTAAATCTCTGTTTCTCTTCTAACATTTGTTATGTTT
GTGTTCTTGCAAATAGGCAGCTCTGCTGTTGTAATTTATGTAGAACAGACAACAGAGTAAGCTGCGTTTAACTTTGAAATTTGCAAGTACGCATGCTTAG
ATTTGAGTTTCCCATTTTAACTTTTGTCCTTGTCAGTTTTACAAAGTGCAAGTGGCTGCTAGGCTGACACGTAGAAGATTGAATGATTTCTCGGAGTTAA
GTTTTGTCCTTTGTC

Start of BLAST Alignment:NT > 76 / Downstream
First 15 bp Perfect Match:NT > 76 / Downstream
BLAST Details:
Query Sequence: 64071_LB1 
Length: (615 letters) 
Database: TIGR AGI BAC Sequences (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

MQC12 60512                                                   1825  2.8e-76   1
F23A5 51668                                                    282  0.00018   1
F3O9 51361                                                     267  0.0010    1
T14A11 60237                                                   264  0.0015    1


MQC12 60512
        Length = 87,799
  Plus Strand HSPs:
 Score = 1825 (279.9 bits), Expect = 2.8e-76, P = 2.8e-76
 Identities = 365/365 (100%), Positives = 365/365 (100%), Strand = Plus / Plus

Query:   249 AACAATATATAGAAATTTAATTCGTCTCTCATACTTCTTTTTGTTTGCCATATTTGACTT 308
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 51756 AACAATATATAGAAATTTAATTCGTCTCTCATACTTCTTTTTGTTTGCCATATTTGACTT 51815

Query:   309 CGTTTTGTTGCCTTTAGTTGTTTAATTTACTTCTTCTGTTGTAGTAGACTCATTGCTAAA 368
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 51816 CGTTTTGTTGCCTTTAGTTGTTTAATTTACTTCTTCTGTTGTAGTAGACTCATTGCTAAA 51875

Query:   369 TCTCTGTTTCTCTTCTAACATTTGTTATGTTTGTGTTCTTGCAAATAGGCAGCTCTGCTG 428
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 51876 TCTCTGTTTCTCTTCTAACATTTGTTATGTTTGTGTTCTTGCAAATAGGCAGCTCTGCTG 51935

Query:   429 TTGTAATTTATGTAGAACAGACAACAGAGTAAGCTGCGTTTAACTTTGAAATTTGCAAGT 488
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 51936 TTGTAATTTATGTAGAACAGACAACAGAGTAAGCTGCGTTTAACTTTGAAATTTGCAAGT 51995

Query:   489 ACGCATGCTTAGATTTGAGTTTCCCATTTTAACTTTTGTCCTTGTCAGTTTTACAAAGTG 548
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 51996 ACGCATGCTTAGATTTGAGTTTCCCATTTTAACTTTTGTCCTTGTCAGTTTTACAAAGTG 52055

Query:   549 CAAGTGGCTGCTAGGCTGACACGTAGAAGATTGAATGATTTCTCGGAGTTAAGTTTTGTC 608
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 52056 CAAGTGGCTGCTAGGCTGACACGTAGAAGATTGAATGATTTCTCGGAGTTAAGTTTTGTC 52115

Query:   609 CTTTG 613
             |||||
Sbjct: 52116 CTTTG 52120


F23A5 51668
        Length = 109,694

  Minus Strand HSPs:

 Score = 282 (48.4 bits), Expect = 0.00018, P = 0.00018
 Identities = 216/366 (59%), Positives = 216/366 (59%), Strand = Minus / Plus