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At1g20200 SYNGENTA 65310

Genomic Sequence (from TAIR)

aaaactagtaaccaaaaagttaatCGCCGTTGCCGGGGATCGAACCCGGGTCACCCGCGTGACAGGCGGGAATACTTACCACTATACTACAACGACattt
gttgctcaatcgtaatgtagaagatataaatactaatatggaaaaaagctgtcttgaacaaaaagaaatatactttttttgttgtgtccaaaaaaaggaa
aatacttaggaaagaaaagagtctcagcagcatcagctcgtccttaaatctgatccatcatcgtcactttctcccggcgaattccgttttatcgatttag
taagctctctgtctttatctctccgtctttctcagtcaacaaattggttgaaacttcaatcggttccttgaaatctcaccggcttagttgcatacttctg
caaaaaccctaaccttgtttttgtctttgtgaagagtaacgagatttgtgcaaagATGACTCAGGACGTGGAGATGAAAGATAACAACACCCCTTCTCAA
TCGATTATCTCTTCTTCTACCTCTACTATGCAGAgtaaatctcgatctgaatgctttaattctcgaactgttttcataattgctaagtttcttctgggtt
ttgacgctttgtttctttgtttcccagATTTGAAGGAGATTGCAGCACTCATCGATACTGGGTCTTACACGAAGGAGGTTCGTCGTATTGCTCGTGCTGT
GCGTCTCACTATAGGGCTTAGGCAGAAACTCACCGGCTCTGTGCTCTCTTCCTTCCTGGATTTTGCTTTG◄GTTCCAGGATCCGAAGCTCACTCTCGCCT
CTCTTCCTTTGTTCCTAAGgtatttgtgggtt►ttacttcttcactttgatgtttcagaattctcaacttggttgttccttttagcatctggctttaggt
tgtttagattcgtgttatggttgttatactttcttttgatgttgtgaatggcaagttatctaacatacaactggcttagagactgaaatattggaacttt
taatggtttagGGTGATGAACATGACATGGAAGTTGATACTGCCTCATCGGCCACACAAGCTGCTCCTTCTAAGCATCTACCTGCAGAGCTCGAGATCTA
CTGCTACTTCATTGTTCTTCTTTTTCTGATTGATCAGAAGAAGTACAACGAGgtaactactgctgtatctttgtgttttgtcataagttacttttctgtt
gtgattttaattttaatgagttgcttttaatcatctcttagGCTAAAGCTTGTTCTTCAGCAAGCATTGCTCGTCTCAAGAACGTCAACCGAAGGACCAT
TGATGTGATAGCATCAAGACTCTACTTTTACTATTCTTTGAGTTATGAGCAAACCGGTGATCTTGCTGAAATTCGCGGgttagtagctcaaagactgttt
tagttttgtactttgtcttacacctcatattaatcagtagctcaatagttaataatctggtcattttttctacctgcagTACTCTTCTTGCGTTGCATCA
TTCTGCAACGCTAAGACACGATGAGCTGGGTCAGgtatgtctttgtttagctcattcagtcggcattcattattttgaaactgttacttgcttcttattc
aaatttatcatgttgaatgcagGAAACCCTTCTGAACCTGTTGCTACGTAACTATTTGCATTACAACCTCTATGATCAGGCAGAGAAGCTAAGATCAAAG
GCACCTCGCTTTGAGGCTCATTCAAACCAACAGgtagctcttggctattccttcatactctaagttttctcggttctctagattctatatttgcatgaaa
actccaatgccacttctgcaatctttccagagaattattttttctattcgctatagcattttccatctaggaggctcttgtgttttcttttcaatcacta
attatttgccgaacttacttgaagtgtccattttgcctgtccttttcatacttctgacaccacctcttaccattaccatcgatctaccttgtgtttttct
ttgctacatatgaattttgttctttttttaacccttcaattgttactgtttcaaaagaataaacagcaagatgacttgtctaaaacattatactttgcag
TTTTGTAGGTACCTTTTCTATCTCGGGAAGATTCGTACTATTCAGCTCGAATATACGGACGCAAAAGAGAGCCTTCTTCAGGCGGCCAGGAAAGCCCCTA
TAGCAGCTTTGGGCTTCAGGATCCAATGCAATAAATGGGCAATTCTGGTTCGTCTACTGCTGGGTGAGATACCAGAGCGTTCTATCTTCACTCAAAAGGG
TATGGAGAAGGCCCTCAGACCCTACTTCGAGCTAACAAATgtgagtccactttcataatcttgctgctgtgacatgtattcattaatttatgagcggata
actaaactggtattagtatgtggtcacttgtgatcttgctacttcacttctgattttaaaccggttcctacttttcacttactggcctatacacctcttc
tgttaaaatctctctgtggtcacttgaaacacaattttgttgctgctgttgttttctgctccttcgaattatattgaacatgaatttctggctgtttact
gttgctttttagagatatatgatgcaactggatagttgatactgattttgatctgaggcttactattaaagctctgttttgattttacagGCGGTTAGGA
TTGGGGACTTGGAGTTGTTTAGGACAGTCCAGGAGAAGTTCTTGGACACATTTGCTCAAGACAGAACGCACAATCTCATCGTGCGACTCCGCCACAATGT
CATCAGGACTGGACTGCGGAACATAAGTATCTCCTACTCAAGAATCTCTTTACCCGATGTTGCCAAAAAGCTGAGGCTCAACTCTGAAAACCCTGTGGCT
GATGCGGAAAGCATCGTGGCAAAGGCCATACGCGACGGAGCTATTGATGCTACAATCGATCACAAAAACGGATGCATGGTCTCCAAAGAAACTGGGGACA
TCTACTCGACGAATGAGCCACAAACTGCGTTCAACTCAAGAATTGCTTTCTGCCTCAACATGCATAACGAAGCTGTCAGAGCATTGAGGTTTCCTCCTAA
CACTCACAAGGAGAAAGAAAGCGATGAGAAGAGGAGAGAGAGGAAGCAACAGGAAGAAGAGCTTGCTAAGCATATGGCTGAGGAAGACGATGATGATTTT
TAGacaaaggtcatctatttcttataagagttgactctccatctgtctcactttttttatgttcacaagtttacttggtactcttctcatcgtctctaag
tgtttctttattgaggatttttatcctcaggaacctttttattactctggtttcactttaggaaaagatatttatcttgttggtttcagaagatcaaatt
caacttcgtttaattgtgtttgggagattttcgcattttaagaattgaacacttgtagttatagctagctgtattaggtgggtcattattagaaattgac
catgtgaaatgctatcatctccaggcgatgcataccattaaaatccacattcataatttacattaaatgatagataagcagtcataaatatctaaagaat
gaaaacaggacatgtgtattgaccattatgtgtattgatacattagtctatctctgccaaactaggtctcattttcttgaataaaacaatctcatgttat
gc

Border_1 (65310_RBD)


TTGACGCTTTGTTTCTTTGTTTCCCAGATTTGAAGGAGATTGCAGCACTCATCGATACTGGGTCTTACACGAAGGAGGTTCGTCGTATTGCTCGTGCTGT
GCGTCTCACTATAGGGCTTAGGCAGAAACTCACCGGCTCTGTGCTCTCTTCCTTCCTGGATTTTGCTTTG

Start of BLAST Alignment:	NT 315 / Exon 3 of 9 (Intron 1 in 5’ UTR)
First 15 bp Perfect Match:	NT 315 / Exon 3 of 9 (Intron 1 in 5’ UTR)
Reverse Complement

BLAST Details:
Query Sequence: 65310_RBD 
Length: (170 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At1g20200 68414.t02257 26S proteasome regulatory subunit ...   850  9.0e-33   1
At1g75990 68414.t08076 26S proteasome regulatory subunit ...   545  5.8e-19   1
At1g21330 68414.t02392 hypothetical protein                    153  0.36      1
At1g40074 68414.t04296 gypsy-like retrotransposon family ...   139  0.87      1


>At1g20200 68414.t02257 26S proteasome regulatory subunit S3, putative (RPN3)
           similar to SP:Q06364 from [Daucus carota]
        Length = 3218

  Plus Strand HSPs:

 Score = 850 (133.6 bits), Expect = 9.0e-33, P = 9.0e-33
 Identities = 170/170 (100%), Positives = 170/170 (100%), Strand = Plus / Plus

Query:     1 TTGACGCTTTGTTTCTTTGTTTCCCAGATTTGAAGGAGATTGCAGCACTCATCGATACTG 60
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   410 TTGACGCTTTGTTTCTTTGTTTCCCAGATTTGAAGGAGATTGCAGCACTCATCGATACTG 469

Query:    61 GGTCTTACACGAAGGAGGTTCGTCGTATTGCTCGTGCTGTGCGTCTCACTATAGGGCTTA 120
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   470 GGTCTTACACGAAGGAGGTTCGTCGTATTGCTCGTGCTGTGCGTCTCACTATAGGGCTTA 529

Query:   121 GGCAGAAACTCACCGGCTCTGTGCTCTCTTCCTTCCTGGATTTTGCTTTG 170
             ||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   530 GGCAGAAACTCACCGGCTCTGTGCTCTCTTCCTTCCTGGATTTTGCTTTG 579


>At1g75990 68414.t08076 26S proteasome regulatory subunit S3, putative (RPN3)
           similar to 26S proteasome regulatory subunit S3 SP:P93768 [Nicotiana
           tabacum (Common tobacco)]
        Length = 2278

  Plus Strand HSPs:

 Score = 545 (87.8 bits), Expect = 5.8e-19, P = 5.8e-19
 Identities = 135/163 (82%), Positives = 135/163 (82%), Strand = Plus / Plus

Border_2 (65310_LBU)


TTACTTCTTCACTTTGATGTTTCAGAATTCTCAACTTGGTTGTTCCTTTTAGCATCTGGCTTTAGGTTGTTTAGATTCGTGTTATGGTTGTTATACTTTC
TTTTGATGTTGTGAATGGCAAGTTATCTAACATACAACTGGCTTAGAGACTGAAATATTGGAACTTTTAATGGTTTAGGGTGATGAACATGACATGGAAG
TTGATACTGCCTCATCGGCCACACAAGCTGCTCCTTCTAAGCATCTACCTGCAGAGCTCGAGATCTACTGCTACTTCATTGTTCTTCTTTTTCTGATTGA
TCAGAAGAAGTACAACGAGGTAACTACTGCTGTATCTTTGTGTTTTGTCATAAGTTACTTTTCTGTTGTGATTTTAATTTTAATGAGTTGCTTTTAATCA
TCTCTTAGGCTAAAGCTTGTTCTTCAGCAAGCATTGCTCGTCTCAAGAACGTCAACCGAAGGACCATTGATGTGATAGCATCAAGACTCTACTTTTACTA
TTCTTTGAGTTATGAGCAAACCGGTGATCTTGCTGAAATTCGCGGGTTAGTAGCTCAAAGACTGTTTTAGTTTTGGACTTTGTCTTACACCTCATATTAA
TCAGTAGCTCAATAGTTAATAATCTGGTCATTTTTTCTACCTG

Start of BLAST Alignment:	NT 376 / Intron 3 of 8 (Intron 1 in 5’ UTR)
First 15 bp Perfect Match:	NT 376 / Intron 3 of 8 (Intron 1 in 5’ UTR)

BLAST Details:
Query Sequence: 65310_LBU 
Length: (643 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At1g20200 68414.t02257 26S proteasome regulatory subunit ...  3206  1.3e-140  1
At1g75990 68414.t08076 26S proteasome regulatory subunit ...  1091  3.7e-44   1
At1g04420 68414.t00383 aldo/keto reductase family protein...   230  0.033     1
At4g17910 68417.t02515 zinc finger (C3HC4-type RING finge...   228  0.048     1


>At1g20200 68414.t02257 26S proteasome regulatory subunit S3, putative (RPN3)
           similar to SP:Q06364 from [Daucus carota]
        Length = 3218

  Plus Strand HSPs:

 Score = 3206 (487.1 bits), Expect = 1.3e-140, P = 1.3e-140
 Identities = 642/643 (99%), Positives = 642/643 (99%), Strand = Plus / Plus

Query:     1 TTACTTCTTCACTTTGATGTTTCAGAATTCTCAACTTGGTTGTTCCTTTTAGCATCTGGC 60
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   641 TTACTTCTTCACTTTGATGTTTCAGAATTCTCAACTTGGTTGTTCCTTTTAGCATCTGGC 700

Query:    61 TTTAGGTTGTTTAGATTCGTGTTATGGTTGTTATACTTTCTTTTGATGTTGTGAATGGCA 120
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   701 TTTAGGTTGTTTAGATTCGTGTTATGGTTGTTATACTTTCTTTTGATGTTGTGAATGGCA 760

Query:   121 AGTTATCTAACATACAACTGGCTTAGAGACTGAAATATTGGAACTTTTAATGGTTTAGGG 180
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   761 AGTTATCTAACATACAACTGGCTTAGAGACTGAAATATTGGAACTTTTAATGGTTTAGGG 820

Query:   181 TGATGAACATGACATGGAAGTTGATACTGCCTCATCGGCCACACAAGCTGCTCCTTCTAA 240
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   821 TGATGAACATGACATGGAAGTTGATACTGCCTCATCGGCCACACAAGCTGCTCCTTCTAA 880

Query:   241 GCATCTACCTGCAGAGCTCGAGATCTACTGCTACTTCATTGTTCTTCTTTTTCTGATTGA 300
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   881 GCATCTACCTGCAGAGCTCGAGATCTACTGCTACTTCATTGTTCTTCTTTTTCTGATTGA 940

Query:   301 TCAGAAGAAGTACAACGAGGTAACTACTGCTGTATCTTTGTGTTTTGTCATAAGTTACTT 360
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   941 TCAGAAGAAGTACAACGAGGTAACTACTGCTGTATCTTTGTGTTTTGTCATAAGTTACTT 1000

Query:   361 TTCTGTTGTGATTTTAATTTTAATGAGTTGCTTTTAATCATCTCTTAGGCTAAAGCTTGT 420
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1001 TTCTGTTGTGATTTTAATTTTAATGAGTTGCTTTTAATCATCTCTTAGGCTAAAGCTTGT 1060

Query:   421 TCTTCAGCAAGCATTGCTCGTCTCAAGAACGTCAACCGAAGGACCATTGATGTGATAGCA 480
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1061 TCTTCAGCAAGCATTGCTCGTCTCAAGAACGTCAACCGAAGGACCATTGATGTGATAGCA 1120

Query:   481 TCAAGACTCTACTTTTACTATTCTTTGAGTTATGAGCAAACCGGTGATCTTGCTGAAATT 540
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1121 TCAAGACTCTACTTTTACTATTCTTTGAGTTATGAGCAAACCGGTGATCTTGCTGAAATT 1180

Query:   541 CGCGGGTTAGTAGCTCAAAGACTGTTTTAGTTTTGGACTTTGTCTTACACCTCATATTAA 600
             ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
Sbjct:  1181 CGCGGGTTAGTAGCTCAAAGACTGTTTTAGTTTTGTACTTTGTCTTACACCTCATATTAA 1240

Query:   601 TCAGTAGCTCAATAGTTAATAATCTGGTCATTTTTTCTACCTG 643
             |||||||||||||||||||||||||||||||||||||||||||
Sbjct:  1241 TCAGTAGCTCAATAGTTAATAATCTGGTCATTTTTTCTACCTG 1283


>At1g75990 68414.t08076 26S proteasome regulatory subunit S3, putative (RPN3)
           similar to 26S proteasome regulatory subunit S3 SP:P93768 [Nicotiana
           tabacum (Common tobacco)]
        Length = 2278

  Plus Strand HSPs:

 Score = 1091 (169.7 bits), Expect = 3.7e-44, P = 3.7e-44
 Identities = 425/622 (68%), Positives = 425/622 (68%), Strand = Plus / Plus