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At5g16715 SYNGENTA 67816

Genomic Sequence (from TAIR)
CTGGTTGGAGACCAAAGGTTATGGGTTAAgagattttgatgttgacctcaagcttccttgtcttctactaaatgtaaaaaacataataatcaaatcttct
tcaactgttaccaatgatatataccttgaatccttgattgcttatatgatgaaaactatattgtgtcatagtggggaagcttgcatataagttttaatgt
accgaccaatcactactaaaattttgaaccatctgcaaattatttaccgtaagctacagagcaattagacgaccattgaaagaacaataattacggaata
cccttcccgtcatccattccttccctcctccgccttaaacccctaccattttgtgtcactagcagcttatctttattttcctgggaatttcaaattcttt
tctcgccccagattttctcgggaatctctgtcgggcgttttcttcagaatctgccATGATTCTCAAAACGGCTTTTTCTCTACCCACACCTACTACTACT
CTATTATCTCCCAGCTCTCCTCACCAGCTCAACACTCTGTTCTTCACTCGCCGACGCCGTCGATTAATTTCCCCATCCCGCTTGAACTCAATTTTCTCGC
AACGTAGATTCTCTTTCTCTGgtacgttcctcgctatttcttttctccagtgtcgaattctgtttccaatcgattttgttggaacttctcttctgttaca
gtaaaaatttattcgaattcctcttttgtattcagCTGCTGCGTCAGGAAATAATGTATTTACTTCTCCCGAGACATCCAAGACCTTTGATTTTTCTTCG
GAGGAGAAGATATACAAATGgtaaatattctactcgtgagttaatggaattattatgagttaagatatatcacaatgcaaataaaaagtgtctcattctt
cccaatgtaacctatgggaacatggatttcttgtgataatgctgatgatcatttggatttgggcatttttagGTGGGAGTCGCAAGGCTACTTCAAACCA
AATTTTGACCAAGGAGGCAGTCCTTTTGTGATACCGATGCCACCTCCTAATGTTACTGGTTCTCTGCACATGGGCCATGCAATGTTTGTCACTCTTGAGg
ttgtggaatccatattgagaataattcacagtcatttgggtattctaatttatgttcctattttcgtttaacctttttagtttgttcgtctagctttagc
tcgtatatactaggcgctttccacacttcttaagttggcttgaaatattgtttagtaattccatgttttctttgtgctgtaactgtgttatactatagtt
ctgctattctcatcgcttttttgttttttacagGATATTATGGTTCGATACAATAGGATGAATGGGAGACCAACACTTTGGCTTCCCGGGACTGATCAT
GCTGGTATTGCCACTCAGgttcatttcctcgttcaaatttcaagcttttttgcatggtatttctactagtgtttgcgtgtttgttttgtgctcactat
gattcttacatcttcttcttcacaatccattgctcttgttcctataccaatctaccacatttactttgtcaaaaagttcttttcttgtagagtataattt
gtatagataatatttgcattgtttggtgaatgtttctgaaatattctactgaagctgcaatatttctttcagTTGGTCGTGGAAAAGATGCTAGCGTCA
GAAGGCATTAAGAGAGTGGACTTAGGTAGAGATGAGTTTACCAAAAGAGTCTGGGAATGGAAGGAAAAgtatgctgtctaatcactgcaatgaataaatt
caaaatatctatttcaaaagtatctgtatgatactctggcggtgtttccattcttcgaacgagtcataagttattgatcttctggaaaactatgaagtat
tttaagcaggagatgattcctgtacatttcttataattgagtaatctgatgttcctgaaccattccaaaggccaatgctttcattaaaagataaaatcca
ctgtacttgtatctgatgtgttaagggtaaggctggaattatagtttgtgtggtttttctcagGTATGGCGGAACCATCACAAATCAGATTAAAAGACTG
GGTGCTTCTTGTGATTGGAGTAGAGAGCGCTTTACTCTTGACGAGCAATTGAGTCgtaaggcatctttcatagtttccatatcttctagatggtgattct
taaagcagaccataggtggtgctcaaagttgactttatatgattttgagtggtattttggaattcaaaaacttcggcaagtaaaatattattcagttgtt
aggtggttcaaaaagtttctcgttgctcttaatttcaaattgtatgtactttcaccttgaatttattccttgattctcttttaccttgaatttgtagGAG
CTGTGGTCGAAGCTTTTGTGAAGCTTCATGACAAGGGGTTGATATATCAAGgtaagctattatgaatttatgtattttggtttctgttgtcgtagctgta
agtagagctgtgctgtactcatacgactattggtgattttggcataatgtttttccagGATCTTACATGGTCAACTGGTCCCCAAATTTACAGACTGCTG
TTTCAGACTTGgtgtgtgatgtatttttatctaacactctgttgtttgattactttttttttctttctaaaagtgactgccttgttcttaccagGAAGTA
GAATATTCGGAGGAACCTGGGTTTCTATACCATATTAAATACCGTGTAGCTGGAAGgtactgtccaactaccagtactaccttcgcctttccttatactt
tgtattttatggacccttatatgtagcaaaattatgtgaaaagataattttttctcacatcaatttgattatgctctgctattagagagttcaaagaagc
attatagtgtttgaaaaacgtatcttatggaggctttagattatgtcgtatgatcattaaaacttgtgaaacacagcgttgggtctgtaagagttgcttc
ggcagtagttaaccttaaattttgctagcaaattattcttggaagatcagtgactacttttgttctgtttatggaaatggctaatctgatctttcctcgt
acagTCCTGATTTTCTTACTATAGCAACGACTCGTCCAGAGACATTGTTTGGTGACGTGGCCCTTGCCGTGCATCCTGAGgtaggtggtgatattttttt
ggtttcgttgataataggacatcgggaatgacagatggcattatcaaagtctgtttgacgtgtttataatttttgaatacacacagagctcagttaacaa
acttagaagcaagtttttttttttgataaaaacttagaagcaaatttaatattagggaggaaaagtatataatgtgtaacacattttattttcttcttcc
ttttgtggttttcatttatatttctattatgtaaaatttaaatgaaattgaaaatgtagtccaggtagtttgtcaggttgctggtccaatttaatgtaag
cagatgtaatctacaaagtagatgctgatttttttggatctcatgtgaatgtagatcgtcttctagtcgtttcctggaataggtgctcttttttgggtat
cgtttgaggattaatgttgtgaaatgtatttggcatctggcagGATGATCGTTACTCAAAATATGTTGGTCAGACAGCAATTGTACCTATGACATATGGT
CGTCATGTCCCAATTATTGCTGATAAGgtgaactccgatctactgcctatgcactctttatgatgtcttttaggatcaaatatctttttcaagtccaaca
aggaaaacgctgcagttgtttttctttgccatttttcgtgctttaagtatcagtattttgctgggaaacttctaagttctagttactcagaaccaagaaa
ctgagtacctcatagtatttcctctgctgctcttcaggcatttctcatcagtttttttgtgtaccatggcagTATGTTGATAAGGATTTTGGGACTGGTG
TTCTGAAGATAAGTCCTGGGCATGATCACAACGATTACCTTCTTGCAAGAAAGCTAGGTCTCCCCATTCTGAATGTGATGAACAAGGATGCAACACTTAA
TGACGTCGCTGGCTTGTTTTGgtaacattttgtttaaaacttatattttagctgaatggaataccctttggttacatcttaacgtccatggcgccttaac
atacatcgtattttcagtggccttgacagGTTTGAGGTGAGGGAGAAACTATGGGCAGATCTTGAGGAGATAGGTTTAGCTGTTAAGAAGGAGCCTCACA
CTTTACGAGTTCCAAGATCTCAGCGTGGTGGAGAAgtatctttctactgacccagcattaatttttcagttcgtttgaagttcataattttctcatgcat
ggtttaactggtgttagGTTATTGAGCCACTTGTAAGCAAACAATGGTTTGTCCACATGGATCCTTTAGCTGAGAAGGCTCTTCTTGCTGTTGAAAACAA
AGAACTTACTATCATACCTGAGAGATTTGAGAAGgtatgcaagcgactggttggtgttattagttactgagctggtatctgtctttttctcctcagtttg
gcaaattctctgaaaggttttgatttttgattgtttacactgcatacttctgcttttgcttttatttcctgtcaccacttgcagATATACAATCACTGGC
TGACAAATATTAAGGATTGGTGCATAAGCCGGCAGCTGTGGTGGGGACATCGCATACCAGTTTGGTATGTGGTTGGAAAGGATTGCGAGGAGGACTACAT
AGTTGCTAAAAGTGCTGAAGAAGCCCTTGAGAAAGCCCTTGAGAAGTATGGAAAAGATGTTGAAATATATCAGGATCCAGATGTTCTTGACACCTGGTTT
TCCAGgttagtttttcatttatttggagattacttttcttctgtgtgtctttgttttagaaatagtcactagtttaatcatgtgaaagtatcagtttcct
cggaatagtcaccatgaattagtgcgtttcctcatatatgacgctgaacatagttttcctcattttgcattaacttcctatattatttatttagatgttt
tttttctgcagCTCGTTGTGGCCATTCAGCACTCTTGGCTGGCCTGATGTAGCAGCGAAGGATTTCAATAACTTCTACCCAACAAATATGTTAGAAACAG
Ggtacattcctttctttttgtcgatcaggaaaacaatggctgctactatcagggctattgttgtaaacccttcactaggaagaaaaaaatttaggaccaa
caacaatttttctgttgccaagaaggcaagaactaatgtctaaattaagtaaaatctgaaaagaaaatgcgctttagttgcagcttgcggttttgaaatt
ctcatcacttcctttaaataacctttctcatttatttggacagGCATGACATACTATTTTTTTGGGTAGCAAGAATGGTTATGATGGGAATAGAATTTAC
AGGGACCGTTCCATTTTCTCATGTCTACCTTCATGGACTTATAAGAGACTCACAAgtaagtgttaggctttttgcttgttctagtaaatgtttttttttc
ttttcttttcaagacgaggtagccactcaaaccttgatcattgaggcttttcatatcattatttctcacttttcccgatatgctattaaacgttaggtaa
cttctaggatttctatttaaggattctcgttctgtatatctccaagtagttaatcagattatttggaattatcagGGGAGAAAAATGTCAAAATCTCTTG
GGAATGTGATTGATCCGCTTGACACAATTAAAGATTTTGGCACTGATGCTCTGAGATTTACGATTGCACTAGGAACTGCTGGTCAGgtttgatctctact
gtgttgaggcagctgaaccatgcttttttgggtaggtttatattaaaatgtcatatttatgtctatattccttgttttccccattacctttttgttagGA
TCTGAACTTATCTACTGAGAGGTTAACTGCAAATAAAGCATTCACCAACAAACTCTGGAATGCTGGAAAGTTTGTGCTTCATAGTCTGCCTAGTCTAAGT
GATACATCTGCTTGGGAGAATTTGCTGGACCTCAAGgtttgcttgtttttccatctccactagaaagtattagggaattgtggtatatttgtcctttcag
tgacagactaagtatggtataaagtcgaaagtcattgataatatgtttagactatctttgtgtttgggatcatgaaccataaaagtctcacaaaaaaacg
aggtagctttaaatataggagattggtcagacctctttccaggagtatctccacctgcataccttttgccattacttaaaacagctgcagagtcgcatca
caagtgataagctaccttaggctgctctcttactatcgatttgttgtgacactttacaataaggattttttctaacattttgcttccttttttttttgac
agTTGGACAAAGAGGAAACCCTGCTTAGTTTACCCTTGCCTGAGTGTTGGGCGgtataatatttttaccctgaagttatccaacagttcaatctgcctac
caccttatattccttttgtattatttcagGTGTCAAAACTCCATATTCTCATTGATTCTGTCACAGCAAGCTATGAGAAGTTGTTCTTTGGGGATGTAGG
AAGAGAAACATATGATTTCTTTTGGAGTGACTTTGCTGATTGgtatcaccacgtcaactcttaataaccaatacacatgtttcttggacttaattgactg
actagtatactctaaagGTATATTGAAGCCAGCAAATCTCGACTGTATGGATCTGGAGGCAATTCAGTTTCTCTAGCTTCTCAGGCGGTTTTGTTATATG
TCTTTGAGAATATACTTAAGCTGTTGCATCCGTTTATGCCGTTTGTAACCGAGGATCTATGGCAGgtaacatgctctcagttttgctatctgtgtatttc
atttttacttaaattggcccttaaccatttacttaggccaactgcactgggcatgattttccttataccttagagtttttgtagtggccgtttttttacc
aaagcatgcttaagctgttatctttgagtttttttttggtagtttagcctcttttgaggatgaaataaaaggaccgaaaacttagttatatatggttgat
actttggtactgctaccaaaaaatgatttcagcttcataaaactgtaattatgaatgcatttataattgcagGCACTTCCCTACAGGAAAGAAGCGCTCA
TTGTGTCTCCATGGCCACAAAATTCACTTCCAAGGAATGTTGAATCAATAAAAAGATTTGAGAATTTACAAGCTCTGgtcagtatgaatttttaccttaa
tatattctgtaaacctcaaacagaaccatacttaaaggaattaatatttatactattaaaagttcagagcatgttgacaccttgcctgttaaatcacagA
CTAGAGCAATTCGAAATGCACGAGCAGAGTATTCAGTGGAACCAGTAAAGCGTATATCTGCCTCAGTTGTTGGAAGTGCAGAGGTCATTGAATATATTTC
Ggtcagtaacttgtggcttcctaacagttaaattatcttacgaataaggttcatcagtttgccttgtgtttccttattaaaaactgtgttcttgtggcgg
tgcagAAAGAGAAGGAGGTATTAGCTCTTCTATCCAGGCTTGACTTAAACAACGTCCATTTTTCCAATGCCCCTCCTGgtataattatctttaatccttt
ttttctcttttcttttcattgtttgcatcttatctcaatatttacattttcgatgattcccttgaatcatcatggtcacaacaaaacttcacatggttgg
tccttcagGTGATGCAAATCTATCAGTCCACCTTGTAGCCAGTGAAGGACTCGAGGCATATCTACCTCTTGCAGCCATGGTTGATATATCATCTGAAGTC
CAACGCATTTCTAAACGTCTCTCCAAGATGCAAACTGAATATGACGCCCTCATAACTCGTCTCAGTTCACCAAAGgtataccataaccatcaaaagagtc
tcttaaacccttgctagaaattgttttcgataagcttattaaacatggtgcattagttttagactctgcttgttttacaacatttttaacgctgaaatat
gtagTTTGTGGAGAAAGCTCCAGAAGAAGTGGTGAGAGGTGTAAAGGAGCAAGTAGAAGAATTAGAAGAGAAGATAAAGTTGACCAAAGCTAGGCTTGAT
TTTCTCAAGTCTACTACTTCTTTAGTGTCTCAGTAAattcaggtagttcttattctctcaagttcggacccgaccaatagcggcccatggaccatttaat
tgtgatcatttgagtcgatgagactacctactattaatatttctgttttttgaggacccaattagtttgaaaatgcccccaaaaatgatcaattgtcaac
tattttaccagtcctattgtcggggcccataatgccttttaagcagaaagtggattttatttttattttattttgacagcagaaagtggatatattatta
atttattatatttgctggttatgttacaattgtatttactattatttaatagcctcaccaactaggtaaattttgtaccaataatcagtaatcagaacaa
catagctcttttcacaaaagtttgtttttgttccaaaattgaaaaatccataaccattttattaggtaaatttattaggtggttgggcgtatggtttcga
taca

Border_1 (67816_LB1)

GCCTTTTCAGAAATGGATAAATAGCCTTGCTTCCTATTATATCTTCCCAAATTACCAATACATTACACTAGCATCTGAATTTCATAACCAATCTCGATAC
ACCAAATCGAATTCAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTATATATNTCCGTATTA
TATATGNAGCNNACACTTAGTANNAATACCATGCATNAAANAGCTTGNANTTTGAACGNGGANNTGNNCCTGAGTGGCNATACCAGCNTGATCAGTCCCG
GGANGCCAAAGTGTTGGTCTCCCATTCATCCTATNGTATCGAACCATAATANCCTGTANNAAACANAAAAGCGATGAGAATAGCNGAACTATAGTATAAC
ACAGTTNC

Start of BLAST Alignment:NT 1216 / Exon 5 of 27
First 15 bp Perfect Match:NT 943 / Exon 4 of 27
BLAST Details:
Query Sequence: 67816_LB1 
Length: (408 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At5g16715 68418.t01777 tRNA synthetase class I (I, L, M a...   815  5.2e-31   1



>At5g16715 68418.t01777 tRNA synthetase class I (I, L, M and V) family protein
           similar to SP|P11931 Valyl-tRNA synthetase (EC 6.1.1.9)
           (Valine--tRNA ligase) (VALRS) {Bacillus stearothermophilus};
           contains Pfam profile PF00133: tRNA synthetases class I (I, L, M and
           V)
        Length = 8052

  Minus Strand HSPs:

 Score = 815 (128.3 bits), Expect = 5.2e-31, P = 5.2e-31
 Identities = 285/409 (69%), Positives = 285/409 (69%), Strand = Minus / Plus

Query:   408 GNAACTGTGTTATACTATAGTTCNGCTATTCTCATCGCTTTTNTGTTTNNTACAGGNTAT 349
             | ||||||||||||||||||||| |||||||||||||||||| |||||  |||||| |||
Sbjct:   824 GTAACTGTGTTATACTATAGTTCTGCTATTCTCATCGCTTTTTTGTTTTTTACAGGATAT 883

Query:   348 TATGGTTCGATACNATAGGATGAATGGGAGACCAACACTTTGGCNTCCCGGGACTGATCA 289
             ||||||||||||| |||||||||||||||||||||||||||||| |||||||||||||||
Sbjct:   884 TATGGTTCGATACAATAGGATGAATGGGAGACCAACACTTTGGCTTCCCGGGACTGATCA 943

Query:   288 NGCTGGTATNGCCACTCAGGNNCANNTCCNCGTTCAAANTNCAAGCTNTTTNATGCATGG 229
              |||||||| ||||||||||  ||  ||| |||||||| | |||||| |||  |||||||
Sbjct:   944 TGCTGGTATTGCCACTCAGGTTCATTTCCTCGTTCAAATTTCAAGCTTTTT--TGCATGG 1001

Query:   228 TATTNNTACTAAGTGTNNGC-TNCATATATAATACGGANATAT-ATACA-A-ATTGACG- 174
             ||||  ||||| ||||  || |   | | |  | |  |  ||| || |  | ||   |  
Sbjct:  1002 TATTTCTACTA-GTGTTTGCGTGTTTGTTTTGTGCTCAC-TATGATTCTTACATCTTCTT 1059

Query:   173 CTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGAATTAACGCCGAATTG 114
             |||  |||| |  ||  | | |||    |  |   ||| |||   ||| ||   |     
Sbjct:  1060 CTTC-ACAA-TCCATTGCTC-TTGTTC-CTATACCAATCTACCACATTTACTTTGTCAAA 1115

Query:   113 AA-TTCGATTTGGTGTATCG-AGA-TTGGTTAT-GAAATTCAGATGCTAGTGTAATGTAT 58
             || |||  |||  ||||  | | | || || || || | | |  ||| | |||  ||   
Sbjct:  1116 AAGTTCT-TTTCTTGTAGAGTATAATTTGT-ATAGATAAT-ATTTGC-ATTGTT-TGG-- 1168

Query:    57 TGGTAATTTGGGAAGATATAATAG-GAAGCAAGGCTATTTATC-CATTT 11
             ||    |||  ||| ||||  ||  |||||     ||||| |  || ||
Sbjct:  1169 TGAATGTTTCTGAA-ATATTCTACTGAAGCTGCAATATTTCTTTCAGTT 1216


Border_2 (67816_LB2)

GCCTTTTCAGAAATGGATAAATAGCCTTGCTTCCTATTATATCTTCCCAAATTACCAATACATTACACTAGCATCTGAATTTCATAACCAATCTCGATAC
ACCAAATCGA:TTCA:TTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTTGTGCTCACTATGA
TTCTTACATCTTCTTCTTCACAATCCATTGCTCTTGTTCCTATACCAATCTACCACATTTACTTTTGTCAAAAAGTTCTTTTCTTGTAGAGTATAAATTT
GGATAGATAATATTTTGCATTTG

Start of BLAST Alignment:NT 995 / Intron 4 of 26
First 15 bp Perfect Match:NT 1023 / Intron 4 of 26
BLAST Details:
Query Sequence: 67816_LB2 
Length: (321 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At5g16715 68418.t01777 tRNA synthetase class I (I, L, M a...   667  2.6e-24   1
At4g15300 68417.t02208 cytochrome P450 family protein sim...   211  0.00099   1
At4g23470 68417.t03163 hydroxyproline-rich glycoprotein f...   207  0.0016    1
At3g14350 68416.t01610 leucine-rich repeat transmembrane ...   190  0.0099    1


>At5g16715 68418.t01777 tRNA synthetase class I (I, L, M and V) family protein
           similar to SP|P11931 Valyl-tRNA synthetase (EC 6.1.1.9)
           (Valine--tRNA ligase) (VALRS) {Bacillus stearothermophilus};
           contains Pfam profile PF00133: tRNA synthetases class I (I, L, M and
           V)
        Length = 8052

  Plus Strand HSPs:

 Score = 667 (106.1 bits), Expect = 2.6e-24, P = 2.6e-24
 Identities = 155/173 (89%), Positives = 155/173 (89%), Strand = Plus / Plus

Query:   147 GCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTTGTGCTCACTATGATTCTTACA 206
             |||  || ||  ||  | || |  ||||   |||||||||||||||||||||||||||||
Sbjct:   996 GCATGGTATTTCTAC-TAGTGTTTGCGTG--TTTGTTTTGTGCTCACTATGATTCTTACA 1052

Query:   207 TCTTCTTCTTCACAATCCATTGCTCTTGTTCCTATACCAATCTACCACATTTACTTTTGT 266
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||
Sbjct:  1053 TCTTCTTCTTCACAATCCATTGCTCTTGTTCCTATACCAATCTACCACATTTACTTT-GT 1111

Query:   267 CAAAAAGTTCTTTTCTTGTAGAGTATAAATTTGGATAGATAATATTTTGCATT 319
             |||||||||||||||||||||||||||| |||| ||||||||||||| |||||
Sbjct:  1112 CAAAAAGTTCTTTTCTTGTAGAGTATAA-TTTGTATAGATAATATTT-GCATT 1162


>At4g15300 68417.t02208 cytochrome P450 family protein similar to Cytochrome
           P450 90C1 (ROTUNDIFOLIA3) (SP:Q9M066) [Arabidopsis thaliana];
           contains Pfam profile: PF00067: Cytochrome P450
        Length = 2026

  Minus Strand HSPs:

 Score = 211 (37.7 bits), Expect = 0.00099, P = 0.00099
 Identities = 149/248 (60%), Positives = 149/248 (60%), Strand = Minus / Plus