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At3g46560 SYNGENTA 68181

Genomic Sequence (from TAIR)

atatcgaattaggattaccgtttagattgttttttttccctcaagtttagtgtatttgatttagaaaggaattcaaatagtttatgagactatttagata
agtaagataaccatatgtatgtaggcgagtattatatatttggtaccgttactatatatcgggcctaattagtaaaacattaggctttaatcaagtctaa
gtaataaagcccaccaggcccaataaagaaaatcgctagggtttaagtcttaagcacagaacaaggaggcgatgaagaattgaatctgtagtcgaaagaa
aagagttgggaaaaaaATGGACGCAAGCATGATGGCTGGACTTGATGGTCTTCCTGAAGAAGACAAAGCCAAAATGGCCTCCATGATCGATCAGCTTCAG
CTCCGTGATAGgttcgtcacttagatcggtttctctgtttttcatttaggattctcccaaaaaaattgacttttagctccttgaaatctttgttcttata
gttcaaagctaattagatttgctttagaatattgattcaaggttagtgtgtacttatttctggctaattatagatctttgggtccttatggttcattgat
aaacaaaagtttggattagagttagtgaaattgttctttaagatgtaactgtgttgtctcattttgtgtgttgttgtcaaaactcacaaaatcttcacat
tatttaggttgtaacaacttatgcaatgcctattgttgttttgtgtttaggccttaactttggctacttttgttgagttttgtttttggtgaatttcagT
TTGAGGATGTACAATTCATTGGTGGAGAGGTGTTTCGTGGACTGTGTTGATA►GCTTCA◄CA◄CGCAAATCTCTGCAGAAACAAGAGGAGACTTGTGTGA
TGCGTTGCGCTGAGAAGTTCCTTAAGCATACGATGCGTGTTGGTATGCGGTTTGCTGAGCTCAATCAGAACGCACCAACCCAAGACTGAtatagtctgct
ttttctgtttggttttttcgtacggtttacgagtccgaaaccagaataaaccggttgttagttgttcaggttactactccgatactgttttttgaacatc
ttgtactgtgaaattattttgactgtatccagttctgatattgatattgacttgtatttcaatggagtctagagaatatcatatcatatcagtattacat
cgaccagttccaaattttcaatgtgtaatattgcttaacatcttgaaactttgttacaaaagaaatcgagctctaatctaatctaaaggtaccttatttg
ttttaatgatatcgacattggtaagtctagtttaatcaaaccgaagttttgctcggaaccgtagatttgatttgtgatcttcgttgaagacgagagaaag
agtgtaactctccaagagaaggaagagaagagaagagatgagatgtagatgaaacaacttgagttctcatcatgatcgtgtacgagtttattaaagttac
aac

Border_1 (68181_LBD)


GCCTTTTCAGAAATGGATAAATAGCCTTGCTTCCTATTATATCTTCCCAAATTACCAATACATTACACTAGCATCTGAATTTCATAACCAATCTCGATAC
ACCAAATCGAATTCAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGAAGCTATCAACACAGTC
CACGAAACACCTCTCCACCAATGAATTGTACATCCTCAAACTGAAATTCACCAAAAACAAAACTCAACAAAAGTAGCCAAAGTTAAGGCCTAAACACAAA
ACAACAATAGGCATTGCATAAGTTGTTACAACCTAAATAATGTGAAGATTTTGTGAGTTTTGACAACAACACACAAAATGAGACAACACAGTTACATCTT
AAAGAACAATTTCACTAACTCTAATCCAAACTTTTGTTTATCAATGAACCATAAGGACCCAAAGATCTATAATTAGCCAGAAATAAGTACACACTAACCT
TGAATCAATATTCTAAAGCAAATCTAATTAGCTTTGAACTATAAGAACAAAGATTTCAAGGAGCTAAAAGTCAATTTTTTTGGGAGAATCCTAAATGAAA
AACAGAGAAACCGATCTAAGTGACGAACCTATCACGGAGCTGAAGCTGATCGATCATGGAGGCCATTTTGGCTTTGTCTTCTTCAGGAAGACCATCAAGT
CCAGCCATCATGCTTGCGTCCATTTTTTTCCCAACTCTTTTCTTTCGACTACAGATTCAATTCTTCATCGCCTCCTTGTTC

Start of BLAST Alignment:	NT 544 / Exon 2 of 2
First 15 bp Perfect Match:	NT 542 / Exon 2 of 2

BLAST Details:
Query Sequence: 68181_LBD 
Length: (781 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At3g46560 68416.t04698 mitochondrial import inner membran...  2936  7.4e-128  1
At5g60040 68418.t06968 DNA-directed RNA polymerase, putat...   233  0.043     1
At3g15810 68416.t01777 expressed protein contains Pfam pr...   216  0.20      1
At5g48910 68418.t05619 pentatricopeptide (PPR) repeat-con...   207  0.47      1


>At3g46560 68416.t04698 mitochondrial import inner membrane translocase (TIM9)
           identical to mitochondrial import inner membrane translocase subunit
           Tim9 [Arabidopsis thaliana] Swiss-Prot:Q9XGX9; contains Pfam domain,
           PF02953: Tim10/DDP family zinc finger
        Length = 902

  Minus Strand HSPs:

 Score = 2936 (446.6 bits), Expect = 7.4e-128, P = 7.4e-128
 Identities = 588/589 (99%), Positives = 588/589 (99%), Strand = Minus / Plus

Query:   768 ATGAAGAATTGAATCTGTAGTCGAAAGAAAAGAGTTGGGAAAAAAATGGACGCAAGCATG 709
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:     1 ATGAAGAATTGAATCTGTAGTCGAAAGAAAAGAGTTGGGAAAAAAATGGACGCAAGCATG 60

Query:   708 ATGGCTGGACTTGATGGTCTTCCTGAAGAAGACAAAGCCAAAATGGCCTCCATGATCGAT 649
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:    61 ATGGCTGGACTTGATGGTCTTCCTGAAGAAGACAAAGCCAAAATGGCCTCCATGATCGAT 120

Query:   648 CAGCTTCAGCTCCGTGATAGGTTCGTCACTTAGATCGGTTTCTCTGTTTTTCATTTAGGA 589
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   121 CAGCTTCAGCTCCGTGATAGGTTCGTCACTTAGATCGGTTTCTCTGTTTTTCATTTAGGA 180

Query:   588 TTCTCCCAAAAAAATTGACTTTTAGCTCCTTGAAATCTTTGTTCTTATAGTTCAAAGCTA 529
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   181 TTCTCCCAAAAAAATTGACTTTTAGCTCCTTGAAATCTTTGTTCTTATAGTTCAAAGCTA 240

Query:   528 ATTAGATTTGCTTTAGAATATTGATTCAAGGTTAGTGTGTACTTATTTCTGGCTAATTAT 469
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   241 ATTAGATTTGCTTTAGAATATTGATTCAAGGTTAGTGTGTACTTATTTCTGGCTAATTAT 300

Query:   468 AGATCTTTGGGTCCTTATGGTTCATTGATAAACAAAAGTTTGGATTAGAGTTAGTGAAAT 409
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   301 AGATCTTTGGGTCCTTATGGTTCATTGATAAACAAAAGTTTGGATTAGAGTTAGTGAAAT 360

Query:   408 TGTTCTTTAAGATGTAACTGTGTTGTCTCATTTTGTGTGTTGTTGTCAAAACTCACAAAA 349
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   361 TGTTCTTTAAGATGTAACTGTGTTGTCTCATTTTGTGTGTTGTTGTCAAAACTCACAAAA 420

Query:   348 TCTTCACATTATTTAGGTTGTAACAACTTATGCAATGCCTATTGTTGTTTTGTGTTTAGG 289
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   421 TCTTCACATTATTTAGGTTGTAACAACTTATGCAATGCCTATTGTTGTTTTGTGTTTAGG 480

Query:   288 CCTTAACTTTGGCTACTTTTGTTGAGTTTTGTTTTTGGTGAATTTCAGTTTGAGGATGTA 229
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   481 CCTTAACTTTGGCTACTTTTGTTGAGTTTTGTTTTTGGTGAATTTCAGTTTGAGGATGTA 540

Query:   228 CAATTCATTGGTGGAGAGGTGTTTCGTGGACTGTGTTGATAGCTTCAAA 180
             ||||||||||||||||||||||||||||||||||||||||||||||| |
Sbjct:   541 CAATTCATTGGTGGAGAGGTGTTTCGTGGACTGTGTTGATAGCTTCACA 589


>At5g60040 68418.t06968 DNA-directed RNA polymerase, putative similar to
           SP|P04051 DNA-directed RNA polymerase III largest subunit (EC
           2.7.7.6) {Saccharomyces cerevisiae}; contains InterPro accession
           IPR000722: RNA polymerase, alpha subunit
        Length = 9680

  Minus Strand HSPs:

 Score = 233 (41.0 bits), Expect = 0.044, P = 0.043
 Identities = 137/211 (64%), Positives = 137/211 (64%), Strand = Minus / Plus

Border_2 (68181_RBU)


CAAACTAGGATAAATTATCGCGCGCGGTGTCATCTATGTTACTAGATCGGGAATTGAGCTCGCGGCCGCCATGGCATATGCTAGCATGCATAATTCCTGC
AGAGCTCGCTTGAGCTTGGATCAGATTGTCGTTTCCCGCCTTCAGTTTAAACTATCAGTGCTTCACACGCAAATCTCTGCAGAAACAAGAGGAGACTTGT
GTGATGCGTTGCGCTGAGAAGTTCCTTAAGCATACGATGCGTGTTGGTATGCGGTTTGCTGAGCTCAATCAGAACGCACCAACCCAAGACTGATATAGTC
TGCTTTTTCTGTTTGGTTTTTTCGTACGGTTTACGAGTCCGAAACCAGAATAAACCGGTTGTTAGTTGTTCAGGTTACTACTCCGATACTGTTTTTTGAA
CATCTTGTACTGTGAAATTATTTTGACTGTATCCAGTTCTGATATTGATATTGACTTGTATTTCAATGGAGTCTAGAGAATATCATATCATATCAGTATT
ACATCGACCAGTTCCAAATTTTCAATGTGTAATATTGCTTAACATCTTGAAACTTTGTTACAAAAGAAATCGAGCTCTAATCTAATCTAAAGGTACCTTA
TTTGTTTTAATGATATCGACATTGGTAAGNCNAGTTTAATCAAACCGAAGTTTTGCTCGGAACCGTAGATTTGATTTGTGATCTTCGTTGAAGACGAGAG
AAAGAGTGTAACTCTCCAAGAGAAGGAAGAGAAGAGAAGAGATGAGATGTAGATGAAACAACTTGAGTTCTCATCATGATCGTGTACGAGGTTATTAAAG
NTACAACTTCTACCGTTTGGCAAAACTCACTATGAGTTATTTTTGGGGAGCAGATCATATTCGGNTTGANTG

Start of BLAST Alignment:	NT 536 / Exon 2 of 2
First 15 bp Perfect Match:	NT 536 / Exon 2 of 2

BLAST Details:
Query Sequence: 68181_RBU 
Length: (872 letters) 
Database: Genes from AGI, Total Genome (DNA);
                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

At3g46560 68416.t04698 mitochondrial import inner membran...  1605  9.5e-68   1
At1g23030 68414.t02579 armadillo/beta-catenin repeat fami...   219  0.18      1
At5g24130 68418.t02573 expressed protein                       188  0.993     1
At3g46570 68416.t04699 glycosyl hydrolase family 17 prote...   185  0.997     1


>At3g46560 68416.t04698 mitochondrial import inner membrane translocase (TIM9)
           identical to mitochondrial import inner membrane translocase subunit
           Tim9 [Arabidopsis thaliana] Swiss-Prot:Q9XGX9; contains Pfam domain,
           PF02953: Tim10/DDP family zinc finger
        Length = 902

  Plus Strand HSPs:

 Score = 1605 (246.9 bits), Expect = 9.5e-68, P = 9.5e-68
 Identities = 321/321 (100%), Positives = 321/321 (100%), Strand = Plus / Plus

Query:   160 GCTTCACACGCAAATCTCTGCAGAAACAAGAGGAGACTTGTGTGATGCGTTGCGCTGAGA 219
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   582 GCTTCACACGCAAATCTCTGCAGAAACAAGAGGAGACTTGTGTGATGCGTTGCGCTGAGA 641

Query:   220 AGTTCCTTAAGCATACGATGCGTGTTGGTATGCGGTTTGCTGAGCTCAATCAGAACGCAC 279
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   642 AGTTCCTTAAGCATACGATGCGTGTTGGTATGCGGTTTGCTGAGCTCAATCAGAACGCAC 701

Query:   280 CAACCCAAGACTGATATAGTCTGCTTTTTCTGTTTGGTTTTTTCGTACGGTTTACGAGTC 339
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   702 CAACCCAAGACTGATATAGTCTGCTTTTTCTGTTTGGTTTTTTCGTACGGTTTACGAGTC 761

Query:   340 CGAAACCAGAATAAACCGGTTGTTAGTTGTTCAGGTTACTACTCCGATACTGTTTTTTGA 399
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   762 CGAAACCAGAATAAACCGGTTGTTAGTTGTTCAGGTTACTACTCCGATACTGTTTTTTGA 821

Query:   400 ACATCTTGTACTGTGAAATTATTTTGACTGTATCCAGTTCTGATATTGATATTGACTTGT 459
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct:   822 ACATCTTGTACTGTGAAATTATTTTGACTGTATCCAGTTCTGATATTGATATTGACTTGT 881

Query:   460 ATTTCAATGGAGTCTAGAGAA 480
             |||||||||||||||||||||
Sbjct:   882 ATTTCAATGGAGTCTAGAGAA 902


>At1g23030 68414.t02579 armadillo/beta-catenin repeat family protein / U-box
           domain-containing protein contains Pfam domain, PF00514:
           Armadillo/beta-catenin-like repeats and Pfam, PF04564: U-box domain
        Length = 2416

  Plus Strand HSPs:

 Score = 219 (38.9 bits), Expect = 0.20, P = 0.18
 Identities = 133/213 (62%), Positives = 133/213 (62%), Strand = Plus / Plus